Mobilization and recovery of energy stores in traíra, Hoplias malabaricus Bloch (Teleostei, Erythrinidae) during long-term starvation and after re-feeding

In some neotropical environments, fishes often experience periods of poor food supply, especially due to extreme fluctuations in rainfall regime. The fish species that experience periods of drought such as the traíra Hoplias malabaricus (Bloch 1794), may stand up to long-term food deprivation. In this study, experiments were performed in order to determine the dynamic of utilization of endogenous reserves in this species during starvation. Adult traíra were both fasted for 30–240 days and re-fed for 30 days following 90 and 240 days of fasting. Glycogen and perivisceral fat were primary energy substrates consumed. During the first 30 days, fish consumed hepatic and muscular glycogen, without exhausting these reserves, and used lipids from perivisceral fat. Hepatic lipids were an important energy source during the first 60 days of starvation and perivisceral fat were consumed gradually, being exhausted after 180 days. Protein mobilization was noticeable after 60 days of fasting, and became the major energy source as the lipid reserves were decreased (between 90 and 180 days). Following the longest periods of food deprivation, fish had utilized hepatic glycogen again. Fish re-fed for 30 days after 90 and 240 days of fasting were able to recover hepatic glycogen stores, but not the other energy reserves.     

Hematological indices and oxidative stress biomarkers response to the starvation of Clarias gariepinus

Starvation effects for five weeks on energy reserves, oxidative stress and hematological indices in Nile catfish Clarias gariepinus was studied. The low protein level in starved fish may result from the lowering effect of prolonged starvation on protein synthesis rather than due to its degenerating protein. Moreover, the elevated level of serum amino acids may promote gluconeogenesis in liver. In addition, the lipid depletion in starved fish may be related to the preferential uses of lipids as an energy to starve fish. Also, unchanged glycemic level may introduce a potent evidence for the presence of active gluconeogenesis, depending on both amino and fatty acids precursors. Also, kidney and liver showed disturbances in metabolites associated with oxidative damage such as elevations in total peroxide, carbonyl protein and DNA fragmentation; these may cause dysfunction to these organs after five weeks of starvation. Total peroxide, carbonyl protein and DNA fragmentation were significantly increased in gills, liver and kidney by 29.9, 30.9 and 30.5; 83.6, 84.6 and 53.7; 82.4, 43.3 and 75.7%, respectively. Starvation induced severe anemia and loss of body weight in the fish. However, white muscle did not show any oxidative damage after five weeks of starvation.     

Physiological and metabolic adjustments of Hoplosternum littorale (Teleostei,Callichthyidae) during starvation

All animals face the possibility of limitations in food resources that could ultimately lead to mortality caused by starvation. The primary goal of this study was to characterize the various physiological strategies that allow fish to survive starvation. A multiparametric approach, including morphological biomarkers, blood plasma metabolites, oxidative stress and energy reserves, was used to assess starvation effects on the fish Hoplosternum littorale. Adult specimens were maintained at four experimental groups: control (fed ad libitum), and starved (not fed) fish for 7 and 28 days. Significant changes were observed not only after 28 days, but also after 7 days of starvation. In the shorter period, the hepatosomatic index as well as plasma triglycerides and glucose were significantly lower in starved fish than in the control ones. These results were accompanied by reduced lipid, glycogen and protein reserves in liver and diminished glycogen content in muscle, suggesting the need of these macromolecules as fuel sources. In addition, increased antioxidant enzyme activities were observed in gills, without evidence of oxidative stress in any of the evaluated tissues. Most significant differences were found in 28-days starved fish: total body weight together with the hepatosomatic index was lower when compared to control fish. The plasmatic metabolites tested (glucose, triglyceride, cholesterol and protein), all energy reserves in liver and glycogen content in muscle decreased in 28-days starved fish. Lipid oxidative damage was reported in liver, kidney and brain, and antioxidant enzymes (GST, GR, GPx and CAT) were activated in gills. According to the multivariate analysis, oxidative stress markers and metabolic parameters were key biomarkers that contributed in separating starved from fed fish. Our study allowed an integrated assessment of the fish response to this particular condition.     

The regulation of endogeneous energy stores during starvation and refeeding in the somatic tissues of the golden perch

Adult golden perch Macquaria ambigua were fed to satiety, starved for up to 210 days, or starved for 150 days then fed to satiety for 60 days to investigate the utilization of energy stores in response to food deprivation and re-feeding. Golden perch sequentially mobilize energy from hepatic tissue, extra-hepatic lipid, and finally muscle components in response to food deprivation. The relative size of the liver was significantly reduced by 30 days after the onset of food deprivation due to the simultaneous mobilization of lipid, protein and glycogen reserves. These stores were renewed rapidly within 30 days by satiety feeding. Mobilization of lipid stores in perivisceral fat bodies occurred between 30 and 60 days of food deprivation. These deposits were also renewed upon re-feeding, although not as rapidly as liver reserves. The glycogen content of the epaxial muscle was reduced by the 60th day of food deprivation but subsequently increased indicating the mobilization of other energy reserves. The concentration of muscle lipid decreased after 90 days of food deprivation. The only significant response in body composition observed in the fish fed to satiety throughout the study was an increase in the relative size of the perivisceral fat bodies. The results of this study suggest that golden perch are well adapted to cope with extended periods of food deprivation, storing energy as perivisceral fat when food is readily available and having a clearly sequential process for mobilizing energy when food is scarce which largely protects the integrity of the musculature.     

Plasma Nesfatin-1 Is Not Affected by Long-Term Food Restriction and Does Not Predict Rematuration among Iteroparous Female Rainbow Trout (Oncorhynchus mykiss)

The metabolic peptide hormone nesfatin-1 has been linked to the reproductive axis in fishes. The purpose of this study was to determine how energy availability after spawning affects plasma levels of nesfatin-1, the metabolic peptide hormone ghrelin, and sex steroid hormones in rematuring female rainbow trout (Oncorhynchus mykiss). To limit reproductive maturation, a group of female trout was food-restricted after spawning and compared with a control group that was fed a standard broodstock ration. The experiment was conducted twice, once using two-year-old trout (second-time spawners) and once using three-year-old trout (third-time spawners). During monthly sampling, blood was collected from all fish, and a subset of fish from each treatment was sacrificed for pituitaries. Pituitary follicle-stimulating hormone-beta (fsh-β) mRNA expression was analyzed with q-RT-PCR; plasma hormone levels were quantified by radioimmunoassay (17β-estradiol and ghrelin) and enzyme-linked immunosorbent assay (11-keto-testosterone and nesfatin-1). Although plasma nesfatin-1 levels increased significantly in the months immediately after spawning within both feeding treatments, plasma nesfatin-1 did not differ significantly between the two treatments at any point. Similarly, plasma ghrelin levels did not differ significantly between the two treatments at any point. Food restriction arrested ovarian development by 15–20 weeks after spawning, shown by significantly lower plasma E2 levels among restricted-ration fish. Pituitary fsh-β mRNA levels were higher among control-ration fish than restricted-ration fish starting at 20 weeks, but did not differ significantly between treatment groups until 30 weeks after spawning. Within both treatment groups, plasma 11-KT was elevated immediately after spawning and rapidly decreased to and persisted at low levels; starting between 20 and 25 weeks after spawning, plasma 11-KT was higher among control-ration fish than restricted-ration fish. The results from these experiments do not provide support for plasma nesfatin-1 as a signal for the initiation of reproductive development in rematuring female rainbow trout.     

Host switch and infestation by Ligula intestinalis L. in a silver bream (Blicca bjoerkna L.) population

Sampling of the fish community was carried out for 20 years in the Mirgenbach reservoir, in North-Eastern France. The prevalence and the mean intensity of Ligula intestinalis (Cestoda) were analysed in roach (Rutilus rutilus) and silver bream (Blicca bjoerkna) populations, the main two infected species. The aim of this study was to investigate the host switch from roach to silver bream and the consequences of L. intestinalis infestation in silver bream, which is an unusual host for this parasite as Ligula parasitism in silver bream appears to be rare. We analysed in detail the relationships between parasitism index (PI), gonadosomatic index (GSI), perivisceral fat abundance (PFA) and condition index (CI) in the silver bream population. In 1998, prevalence of L. intestinalis highlighted a clear host switch from roach to silver bream. In the silver bream population, young fish were the most severely infected and the impact of plerocercoids appeared to be different depending on the host sex. In male silver bream, plerocercoids drew energy from fat reserves even if GSI was also slightly impacted. On the contrary, in females energy was diverted from gonad maturation rather than from perivisceral fat reserves. No significant difference was observed in terms of CI in either sex.     

Winter energy allocation and deficit of juvenile walleye pollock <Emphasis Type="Italic">Theragra chalcogramma</Emphasis> in the Doto area,northern Japan

Seasonal energy allocation and deficits of marine juvenile fishes have considerable effects on their survival. To explore the winter survival mechanism of marine fishes with low lipid reserves in their early life, juvenile walleye pollock Theragra chalcogramma were collected along the continental shelf of northern Japan over a 2-year period, and energy allocation and deficit patterns were compared between wild and laboratory-starved fish. Contrary to expectations, wild fish generally continued to accumulate protein mass and concurrently tended to reduce lipid mass from late autumn through winter. The most plausible explanation for the continuous structural growth is that juvenile pollock give priority to reducing mortality risk from size-selective predators under quasi-prey-limited conditions. Exceptionally, inshore small fish reduced both constituents during a winter. The inshore fish consumed 2.5 times more lipid energy than protein energy in November–December, but protein was more important than lipids as a source of energy in December–January and in February–March. However, dependence upon protein reserves was lower for the wild fish than for the laboratory-starved fish, suggesting milder nutritional stress of the wild fish than that observed in the starvation experiment. Moreover, the lipid contents of mortalities in the starvation experiment were mostly <1%, whereas few wild fish had such lipid contents in the field. These results suggest that juvenile pollock are able to avoid both starvation and predation by accumulating protein reserves.     

The effects of long-term food deprivation on respiration and haematology of the neotropical fish Hoplias malabaricus

Growth of adult traíras Hoplias malabaricus ceased and body mass ( M ) decreased during starvation periods of 30, 60, 90, 150, 180 and 240 days. Hepatic reserves were mobilized in fish starved for 30 days, but liver mass of fish starved for longer periods was not significantly different from those starved for 30 days. Perivisceral fat bodies were consumed gradually, being completely exhausted after 240 days of food deprivation. Length of starvation was associated with a significant decrease in the oxygen uptake ( V o₂). In spite of this reduction, the respiratory frequency ( f _R) was kept nearly constant during the starvation periods. The haematocrit and the number of red blood cells decreased after 150 and 240 days of starvation, respectively. These parameters did not recover after refeeding (after 90 and 240 days of starvation). This hypometabolic state in response to food deprivation contributed to energy conservation during these periods. Traíras can survive food deprivation for periods of up to 180 days without reductions in metabolism and when they do become hypometabolic, normal metabolic rates are rapidly restored upon refeeding.     

Nutritional condition of Anguilla anguilla starved at various salinities during the elver phase

The effects of food deprivation and environmental salinity (<1, 10 and 20) on survival, fish morphology, organization of the digestive system and body lipid reserves in European eel Anguilla anguilla during the transition from glass eel to elver, were evaluated. Fasted elvers kept in fresh water were able to withstand starvation for >60 days, while those in brackish environments (salinity 10 and 20) reached the level of irreversible starvation at 37 and 35 days, respectively. The high level of lipid reserves contained in liver inclusions and the abdominal cavity (perivisceral deposits) in elvers might explain their long resistance to starvation and differences in fasting tolerance under different salinities. Fasting resulted in a significant reduction of the elvers' condition factor and body depth. There were severe histopathological changes in the digestive system and musculature, such as the alteration of the liver organization, and hepatic glycogen and lipid content, shrinkage of enterocytes and reduction of their height, pancreas degeneration, autolysis of the oesophageal and intestinal mucosa and disarrangement of myofibrils and degeneration of trunk musculature. Degeneration of the oesophageal and intestinal mucosa as a consequence of fasting might have impaired digestive and osmoregulatory functions in feed‐deprived fish, directly affecting the tolerance to starvation and survival. Length of food deprivation was associated with a significant increase in mortality, coefficient of variation, cannibalism and point of no return at high salinities. Mortality was dependent on food deprivation and salinity concentrations. Environmental salinity directly influenced the ability of elvers to withstand starvation; once glass eels metamorphosed into elvers, they tolerated starvation better in fresh water than in brackish environments.     

Biochemical composition,condition index,and energy value of Ostrea peulchana (D'Orbigny): relationships with the reproductive cycle

Seasonal changes in body weight, biochemical composition, energy value, and condition index of Ostrea puelchana (D'Orbigny, 1846) from Laguna Punta Cero (Chubut, Argentina), were studied between February 1983 and April 1984. These values were determined for the soft tissues of the whole animal and, separately, from the adductor muscle, digestive gland-gonad complex, and a fraction containing all remaining organs. The main muscle components were proteins whereas glycogen and lipids were stored in the digestive gland-gonad complex. Weight and energy reserves decreased in winter during the beginning of the reproductive cycle and starvation period. In spring, there was a rapid growth of the gonad coincident with an increase in glycogen, lipid, and protein contents. In summer the glycogen content decreased with spawning, and proteins showed the highest values. The glycogen index appears to be suitable as an indicator of the physiological state. During the post-spawning period, in the autumn, the animals attained their best condition in quantity of flesh with a shigh energy value.     

Reproductive patterns of blacktail comber (Serranus atricauda,Serranidae) from south-west Portugal seamounts

The knowledge of the reproductive strategy is essential for the comprehensive understanding of the population dynamics of any fish species. This study intends to define the spawning season, verify the type of fecundity regulation and estimate relative annual fecundity for Serranus atricauda inhabiting the south-west of Portugal seamounts (Gorringe, Ampère and Lion). Monthly samples, between September 2010 and December 2011, were obtained from commercial vessels. This species is synchronously hermaphroditic, and mature individuals were found all year round, with gonadosomatic index peak from February to September. No clear relation between hepatosomatic index and Fulton’s condition factor and gonadal development was found, and therefore, it is probable that reproductive success depends more on environmental conditions and food availability during the spawning season than on body energy reserves. No gap exists between pre-vitellogenic and vitellogenic oocytes and the oocyte size-frequency distribution during the main spawning season show no dominant cohort progressing through time. The number of standing stock of advanced vitellogenic oocytes showed an increase trend along the spawning season decreasing only at its end and the relative intensity of α-atresia was always low throughout this period with an increase towards the end. All these criteria corroborate the indeterminate fecundity type assigned to S. atricauda in previous studies. Mean relative annual fecundity estimated was 5,655 ± 4,321 oocytes per gram of gutted weight.     

Spatial and Temporal Relations between Fluvial and Allacustrine Yellowstone Cutthroat Trout, Oncorhynchus clarki bouvieri, Spawning in the Yellowstone River, Outlet Stream of Yellowstone Lake

Yellowstone cutthroat trout (YCT), Oncorhynchus clarki bouvieri, that spawn in the outlet of Yellowstone Lake show two potamodromous migration patterns, fluvial and allacustrine. The main purpose of this study was to determine whether those fluvial and allacustrine YCT represent reproductively isolated stocks. Redd surveys indicated spawning occurred during about 5 consecutive weeks between late May and mid-July 1993–1995. Lake fish (N=6), defined as radiotagged YCT that entered Yellowstone Lake after the spawning period (i.e. allacustrine pattern), were found in the river between the lake outlet (river kilometer [Rkm] 0) and Rkm 20.0 during spawning. Probable lake fish (N=28; tagged YCT that were last detected near the lake outlet) were found between Rkm 0 and Rkm 22.5 during spawning. River fish (N=4; tagged YCT that remained in the river when annual tracking concluded in fall, i.e. fluvial pattern) were found between Rkm 1.1 and Rkm 18.0 during spawning. Fidelity to spawning areas used between consecutive years was suggested by one of five lake fish and the single river fish for which data were available. Spatial overlap in spawning and a lack of temporal separation between the life-history types during spawning suggested that fluvial and allacustrine YCT were not reproductively isolated. Radiotagging, as well as visual observations made annually from boats during April and May, indicated fluvial YCT overwintered downstream from Rkm 14 and were few, probably on the order of 10% of all YCT that spawned in the Yellowstone River.     

Effect of short-term starvation on hepatopancreas and plasma energy reserves of the Pacific white shrimp (Litopenaeus vannamei)

Crustaceans are forced to fast during molting. Several physiological, metabolic and behavioral changes have been associated with starvation. Although some of these changes have been well studied, knowledge of the dynamics of fuel reserves during the molting process is limited. To understand the effects of short-term hunger stress on energy reserves, intermolt shrimp Litopenaeus vannamei were starved up to five days. This period corresponds to the normal time that juvenile shrimp starve during molting, since they can not eat. Glucose, glycogen, total soluble protein, total lipids, sterols, and acylglycerides were measured in plasma and hepatopancreas. The same metabolic substrates were measured in organisms that were fed after 96 h of starvation. It is widely accepted that protein is the main energy reserve used by shrimp to deal with starvation. However, under short-term starvation a rapid decrease of plasma and hepatopancreas glucose and an important decrease in hepatopancreatic glycogen were detected. Additionally, acylglycerides content in hepatopancreas decreased significantly at later times, while protein in plasma and hepatopancreas remained fairly constant during the experiment. This study may help understand some aspects of the nutrition physiology of the Pacific white shrimp related to its biology.     

Thermal heterogeneity,migration, and consequences for spawning potential of female bull trout in a river–reservoir system

The likelihood that fish will initiate spawning, spawn successfully, or skip spawning in a given year is conditioned in part on availability of energy reserves. We evaluated the consequences of spatial heterogeneity in thermal conditions on the energy accumulation and spawning potential of migratory bull trout (Salvelinus confluentus) in a regulated river–reservoir system. Based on existing data, we identified a portfolio of thermal exposures and migratory patterns and then estimated their influence on energy reserves of female bull trout with a bioenergetics model. Spawning by females was assumed to be possible if postspawning energy reserves equaled or exceeded 4 kJ/g. Given this assumption, results suggested up to 70% of the simulated fish could spawn each year. Fish that moved seasonally between a cold river segment and a warmer reservoir downstream had a greater growth rate and higher propensity to spawn in a given year (range: 40%–70%) compared with fish that resided solely in the cold river segment (25%–40%). On average, fish that spawned lost 30% of their energy content relative to their prespawn energy. In contrast, fish that skipped spawning accumulated, on average, 16% energy gains that could be used toward future gamete production. Skipped spawning occurred when water temperatures were relatively low or high, and if upstream migration occurred relatively late (mid‐July or later) or early (early‐May or earlier). Overall, our modeling effort suggests the configuration of thermal exposures, and the ability of bull trout to exploit this spatially and temporally variable thermal conditions can strongly influence energy reserves and likelihood of successful spawning.     

Seasonal changes in serum lipid composition of the plaice, Pleuronectes platessa L.

Seasonal changes in the major lipid classes of plaice serum were examined in fish caught off the north-east coast of Scotland. Both male and female plaice were sampled monthly between June 1983 and May 1984. Total lipid, total cholesterol, free cholesterol, triglycerides, phospholipids and non-esterified fatty acids were measured using commercial kits validated against standard reference methods. There were no significant differences in lipid levels between male and female fish and, although there were real differences between months for each lipid class, there was no regular pattern over the 12-month period. All the lipid class values, however, reached a maximum in September, which could be related to the increase in feeding after spawning. The smaller peak in February could be associated with the mobilization of lipid reserves for gonadal development and to overcome the effect of starvation during spawning.     

Digestive enzyme activity of the Japanese grenadier anchovy Coilia nasus during spawning migration: influence of the migration distance and the water temperature

In this study, we investigated the activity levels of two major digestive enzymes (pepsin and lipase) in the commercially important Japanese grenadier anchovy Coilia nasus during its upstream migration to analyse the digestive physiological responses to starvation and to analyse the influence of the water temperature on enzyme activity. Water temperature had a significant effect on pepsin activity, while long-term starvation resulted in a significant decrease in pepsin activity. As starvation continued, however, a slight increase in pepsin activity between the Wuhu (440 river km) and Anqing (620 river km) regions may indicate that C. nasus had refeeding behaviour due to its large expenditure of energy reserves. In contrast, lipase activity was not significantly affected by the water temperature but the effect of fasting increased as much as 13% of lipase activity from the Chongming region (20 river km) to Anqing region, suggesting that the stored lipids of grenadier anchovy were mobilised to meet energy requirements of upstream migration activity and gonad development. Lipid mobilisation activated lipoprotein lipase (LPL; proteins with lipase activity) to hydrolyse triacylglycerides (TAG), which is the first step of lipid assimilation and obtained energy from fatty acids under fasting conditions. Therefore, the increased lipase activity is attributed mainly to the lipase that is involved in endogenous lipid hydrolysis. Grenadier anchovy appears to adapt to long-term starvation during migration and the increased lipase activity may indicate a crucial effect on lipid metabolism. This study demonstrated that distinct alterations occur in pepsin and lipase activities during the spawning migration of grenadier anchovy due to exogenous nutrition and endogenous metabolism. Furthermore, it provides a basis for further research on the digestive physiology and energy metabolism in this species.     

Use of radio telemetry and electrofishing to assess spawning by transplanted Atlantic salmon

The river Ingdalselva, which drains to the Trondheims fjord, has no local salmon population due to an impassable waterfall 500 m upstream from the outlet. In the period 1994–97, a total of 31 mature Atlantic salmon (19 females and 12 males) from the rivers Orkla (1994–96) and Vigda (1997) were radio-tagged and released in the river Ingdalselva before spawning. The main goal of the project was to find out whether the fish would stay and spawn in the river, and if the observations during the spawning period could be used to indicate where spawning had taken place. Some fish left the river shortly after release, but 77% of the fish stayed in the river during the spawning period in October. Most of the females (74%) spawned in the river, including multi-sea winter salmon of approximately 10 kg. Some fish remained at the site of the release, while others migrated downstream to hiding places where they stayed until spawning. Long distance upstream migrations were not observed. The observations of the fish during the spawning period were used to identify spawning areas. Electrofishing for salmon fry in the spawning areas the following year in all cases produced fry, and in two of the years salmon fry were also found in areas where no spawning activity had been registered. Radio-tracking was an efficient method for determining whether transplanted salmon would remain in a `new'n river during the spawning season and for locating spawning areas, particularly when the fish were tracked daily.     

Effect of starvation and refeeding on digestive enzyme activities in juvenile roach, Rutilus rutilus caspicus

We evaluated the effects of starvation and refeeding on digestive enzyme activities in juvenile roach, Rutilus rutilus caspicus. Fish were divided into four feeding groups (mean mass 1.68 ± 0.12 g). The control group was fed to satiation twice a day throughout the experiment with formulated diet (SFK). The other three groups were deprived of feed for 1(S1), 2(S2), and 3(S3) weeks, respectively, and then fed to satiation during the refeeding period. The results showed that trypsin specific activity was not affected significantly either by starvation or refeeding, in all experimental groups. Chymotrypsin specific activity did not change significantly in S1 fish during the experimental period. In S2 and S3 fish no significant changes were observed during the starvation period. Upon refeeding, the activity increased in S2 fish, while it decreased in S3 fish. Amylase specific activity decreased significantly during the starvation period in all experimental groups. Upon refeeding, the activity increased. Alkaline phosphatase specific activity did not change significantly during the experiment period in S3 fish, while it showed significant changes during the starvation and refeeding period in the S1 and S2 fish. Starvation also had a significant effect on the structure of the intestine.     

Effects of starvation and feeding on tissue Nα -acetylhistidine levels in Nile tilapia Oreochromis niloticus

The effects of long-term starvation and feeding on Nα, -acetylhistidine (NAcH) levels in skeletal muscle, brain and lens of Nile tilapia Oreochromis niloticus were examined. A drastic decrease in NAcH level of skeletal muscle during starvation was observed. This compound disappeared from skeletal muscle of the fish after 8 weeks of starvation, and was completely restored to the pre-starvation level (4–5 μmol/g muscle) by the following 8 weeks of feeding. The activities of N-acetylhistidine deacetylase (NAcHDE) and histidine acetyltransferase (HISAT) were very weak, but detectable in skeletal muscle of the species. It is supposed that the change of the NAcH level in skeletal muscle during starvation and feeding appears as a result of the degradation by NAcHDE and of the synthesis by HISAT.     

Constraints of body size and swimming velocity on the ability of juvenile rainbow trout to endure periods without food

The hypothesis that body size and swimming velocity affect proximate body composition, wet mass and size‐selective mortality of fasted fish was evaluated using small (107 mm mean total length, L _T) and medium (168 mm mean L _T) juvenile rainbow trout Oncorhynchus mykiss that were sedentary or swimming ( c . 1 or 2 body length s⁻¹) and fasted for 147 days. The initial amount of energy reserves in the bodies of fish varied with L _T. Initially having less lipid mass and relatively higher mass‐specific metabolic rates caused small rainbow trout that were sedentary to die of starvation sooner and more frequently than medium‐length fish that were sedentary. Swimming at 2 body length s⁻¹ slightly increased the rate of lipid catabolism relative to 1 body length s⁻¹, but did not increase the occurrence of mortality among medium fish. Death from starvation occurred when fish had <3·2% lipid remaining in their bodies. Juvenile rainbow trout endured long periods without food, but their ability to resist death from starvation was limited by their length and initial lipid reserves.