Nutritional supplementation improves ovulation and pregnancy rates in female goats managed under natural grazing conditions and exposed to the male effect

Two experiments were conducted to determine if nutritional supplementation improved ovulation and pregnancy rates in female goats managed under grazing conditions and submitted to the male effect. In Experiment 1, one group of does did not receive nutritional supplementation, while the other group was supplemented daily for 7 days starting at the time when the males were introduced to the females. The ovulation rate at the second male-induced ovulation was greater (P < 0.05) in supplemented (2.0 ± 0.1) than in non-supplemented (1.6 ± 0.1) does. For Experiment 2, female goats were supplemented for 0, 7, 14 or 28 days, starting 9 days following buck introduction. The proportion of does that were pregnant in the group supplemented for 28 days was greater (P < 0.05) than in the non-supplemented group, but did not differ from 14-day and the 7-day supplemented groups. The proportion of pregnant does was greater (P < 0.05) in the group supplemented for 14 days compared to the group supplemented for 7 days and the non-supplemented group. These latter two groups did not differ (P > 0.05). In conclusion, feed supplementation for 7 days, starting at the time when males were introduced increased ovulation rate and feed supplementation for 14 or 28 days starting 9 days after males were introduced improved pregnancy rates in goats managed under grazing conditions and exposed to males.     

Maintaining contact with bucks does not induce refractoriness to the male effect in seasonally anestrous female goats

A study was performed to determine if complete separation between sexes is a pre-requisite to stimulate sexual activity of anovulatory female goats by male exposure in mid-anestrus when reproductive activity is greatly inhibited. The response to the male was studied in anovulatory goats which had either been in contact with bucks before the stimulation (n = 22; CONT), or totally isolated from bucks (n = 22; ISOL). At the time of male exposure, the bucks from the CONT groups were removed and females of both groups (CONT and ISOL) were divided into two subgroups (n = 11) exposed to novel bucks (n = 1 per subgroup), either in sexual rest (sexually inactive; SI) or in which sexual activity had been induced by a photoperiodic treatment (sexually active; SA). The sexual behavior of the SA bucks during the first 5 days of stimulation was greater than that of SI bucks (P < 0.01). Regardless of whether females had been (82%) or not (91%) exposed to bucks before teasing, most goats stimulated by SA males displayed estrous behavior and ovulation in the first 15 days following stimulation, whereas no female from the two subgroups exposed to SI bucks showed estrous behavior and only one of them ovulated (P < 0.02). These results indicate that previous isolation of seasonally anovulatory goats from bucks is not required to stimulate their reproductive activity by the male effect, if sexually active bucks are used.     

Nutritional supplementation improves the sexual response of bucks exposed to long days in semi-extensive management and their ability to stimulate reproduction in goats

In confined management systems, well-nourished bucks rendered sexually active by exposure to long days are efficient in fertilizing out-of-season goats. However, underfeeding is common in semi-extensive management systems and may reduce the reproductive efficiency of bucks. The objective of the present study was to determine whether nutritional supplementation improved the sexual activity of bucks submitted to long days in semi-extensive management systems and their ability to stimulate the reproduction of goats in semi-extensive or confined conditions. In experiment 1, three groups of bucks were placed in different flocks and grazed daily with females for 7 h. Each day after grazing, males were separated from females and moved into open pens. One group did not receive any treatment (control group; n = 6). Two other groups were submitted to artificially long days from 15 November to 15 January. From 16 January, one group did not receive nutritional supplementation (long-day group; n = 5), whereas bucks from the other group each received 600 g of a commercial concentrate (long-day + supplementation group; n = 5). The fourth group was kept in confined conditions, exposed to long days and fed alfalfa hay (long-day confined group; n = 6). On 26 March, anovulatory goats from other flocks were assigned to four groups (n = 27 each) and confined separately in open pens. Three bucks of each group were housed with the females. Pregnancy rates were greater in the goats housed with the long-day group than those housed with the control group (P < 0.01). However, pregnancy rates did not differ between the long-day confined group (89%) and long-day + supplementation group (70%; P = 0.09), but these rates were greater than those from the long-day (37%) and control groups (0%; P < 0.05). In experiment 2, two groups of males (n = 3 each) were incorporated into two flocks under semi-extensive management and grazed daily with females for 7 h. One group of males did not receive any treatment (control group). The other group was submitted to long days and nutritional supplementation as in experiment 1 (long-day + supplementation group). Males remained with females during the whole study. The pregnancy rate was greater in the goats joined by males of the long-day + supplementation group (78%) than in those from the control group (0%; P < 0.001). We conclude that long days and nutritional supplementation improve the ability of bucks kept in semi-extensive management to stimulate reproduction of out-of-season goats in confined or semi-extensive management systems.     

Male effect in seasonally anovulatory lactating goats depends on the presence of sexually active bucks,but not estrous females

A study was conducted in subtropical northern Mexico (26 degrees N) to determine whether the presence of estrous females can improve the response of seasonally anovulatory goats to the introduction of bucks in the group. The induction of estrous activity was studied in three groups of anovulatory lactating goats during seasonal anestrus. These females were of the Mexican Creole breed. In the control group (sexually inactive (SI), n = 20), two control (SI) bucks exposed to normal seasonal daylength variations were used. In the second group (SI + E, n = 20 + 3), two control males were also used, but in addition, three females of the group were in estrus at the time of male introduction. In the third group (sexually active, SA + E, n = 19 + 4), anovulatory females were exposed to two bucks made sexually active by exposure to 2.5 months of long days (16L:8D) followed by two subcutaneous 18 mg melatonin implants, and four estrous females were also present when introducing the bucks. In all groups, males were introduced on 15 March and estrous detection was conducted twice daily for 15 days. The sexual activity of the bucks was observed from 08:00 to 10:00 h during the first five days of exposure to females. More females displayed estrous behavior in the first 15 days following the introduction of the males in the SA + E group (18/19) as compared with the SI or SI + E groups (2/20 and 0/20, respectively; P < 0.001). No difference was observed between the two latter groups. Thirteen females of SA + E group showed a second estrus between days 6 and 11 (short estrous cycle duration: 5.4 +/- 0.4 days). By contrast, in the SI group none showed a second estrus. The sexual behavior of the males in the SA + E group was greater as compared with that of the males in SI and SI + E groups (over 80% of the total sexual activity recorded in the three groups; P < 0.001). By contrast, no differences were found between SI and SI + E males. These results indicate that the presence of estrous females alone at the time of buck introduction is not sufficient to induce an adequate stimulation of seasonally inactive males. The use of sexually active bucks is necessary to induce reproductive activity in anovulatory females, whereas preparation of the bucks with long days followed by melatonin implants allows them to gain such a capacity.     

Influence of vitamins A, D3 and E status on post-mortem meat quality in steers under winter housing or pasture finishing systems

We investigated the influence of Swedish recommended vitamins A, D3 and E supplementation levels on muscle tenderness and fatty acid (FA) composition under indoor or outdoor finishing programmes. Swedish Red breed steer calves were divided into vitamin supplemented (n = 12) and non-supplemented (n = 15) groups while on pasture prior to the finishing period. This trial began at the beginning of the winter housing period during which the steers were fed a 55 : 45 dry matter barley : grass silage diet indoors. The indoor finished group was comprised of vitamin supplemented (n = 6) and non-supplemented (n = 8) steers slaughtered after about 155 days on feed. Vitamin supplemented steers were provided with 100 g mineral supplement providing 400 000 IU vitamin A, 100 000 IU D3 and 3000 IU E daily as recommended for Swedish production practices. In spring, outdoor finished vitamin supplemented (n = 6) and non-supplemented (n = 7) steers grazed semi-natural grassland for an additional 120 days before slaughter. During pasture, vitamin supplemented steers had free-choice access to a mineral supplement containing vitamins A, D3 and E. The mineral supplement for the non-supplemented steers did not contain vitamins A, D3 and E and was provided at the same amount as the vitamin supplemented steers. Shear force values were similar between vitamin supplemented and non-supplemented steers after ageing 2, 7 and 14 days within indoor and outdoor finishing programmes. The shear force values had decreased by 14 days of ageing within all programmes. The μ- and m-calpain activity did not differ between vitamin supplemented and non-supplemented steers for either the indoor or outdoor finishing programmes. The calpastatin activity was higher for the indoor, vitamin supplemented steers. Indoor finished vitamin supplemented steers had a greater proportion of C18:1c-9 and total monounsaturated fatty acids, whereas the non-supplemented steers had a greater proportion of total saturated fatty acids. We concluded that the meat quality from steers not receiving vitamin supplementation was similar to that of steers receiving vitamins A, D3 and E supplementation at Swedish recommended levels under indoor and outdoor finishing programmes.     

The reproductive response to the male effect of 7- or 10-month-old female goats is improved when photostimulated males are used

The exposure of adult, female, Mediterranean goats during anoestrus to males with induced sexual activity via photostimulation, induces a very high percentage of ovulations. The present work examines the ability of photostimulated bucks to improve the male effect-induced reproductive response of young does over that induced by non-stimulated bucks. A 2×2 factorial experiment was designed, consisting of doe age and buck photoperiod treatments. During seasonal anoestrus, 41 does aged 7 (n=19) or 10 (n=22) months were subjected to the male effect on 10 April; half of each group was exposed to males rendered sexually active by prior exposure to 3 months of long days (16 h of light/day) from 31 October (PHOTO bucks), and half to males maintained under the natural photoperiod (CONTROL bucks). Oestrous activity was recorded daily by direct visual observation of the marks left by male-worn marking harnesses over the 32 days following the bringing of the sexes together (introduction). Doe body weight and body condition were determined weekly. Ovulation was detected by measuring plasma progesterone concentrations twice per week over the 3 weeks after introduction. The ovulation rate was assessed by transrectal ultrasonography. Fecundity, fertility, prolificacy and productivity were also determined. The interaction doe age × buck photoperiod treatment had no effect on any outcome. The percentage of females showing ovulation or oestrus was higher in the does exposed to PHOTO bucks (85% v. 43% for those exposed to CONTROL bucks) they also showed higher fertility (75% v. 43%) and productivity (1.05±0.17 v. 0.57±0.16 kids born per doe serviced) (all P values at least P<0.05). The 10-month-old group showed higher percentage of females showing ovulation, oestrus, fertility and productivity than the 7-month-old does after the male effect (females showing ovulation: 82% v. 42%; showing oestrus: 73% v. 42%; fertility: 73% v. 42% and productivity: 1.09±0.17 v. 0.47±0.14 goat kids born per doe serviced; respectively, all P values at least P<0.05). The present results show that the use of photostimulated males improves the reproductive performance of 7- and 10-month-old does, and may contribute towards increasing their productivity and lifetime reproductive performance.     

Mating behavior of bucks and does in goat operations under range conditions

The objectives of this study were to characterize the mating capacity of bucks under range condition and assess the effect of mating frequency on flock fertility. Two adjacent flocks of crossbred goats (Criolloxdairy breeds; n=70 does, 5 bucks and 141 does, 4 bucks) were used in this study. The mating period was 16 and 20 days for each flock, respectively, in January and February, 1996. Mating activity of bucks and does was recorded day and night during the first 11 days of the breeding period. The combined data of both flocks showed a strong relationship between number of goats in estrus and the average services of bucks (r=0.95; P<0.01). With an excess of estrous does, bucks on average copulated 9.1 times daily during the first 11 days of the mating period. Considering both flocks combined, does copulated on average 4+/-1.8 times through the estrus period, and they did so with an average of 2.2+/-1.3 different bucks. Sexual activity of bucks was greatest from sunset to midday. Number of services from different bucks did not affect pregnancy rate (81.4% for goats serviced by 1-2 bucks and 77.8% for goats serviced by 3-4 different bucks; chi(2)=0.25; P7 services, respectively; chi(2)=0.67; P0.05). Mean+/-S.D. daily liveweight loss of bucks during the entire mating period was 547+/-197 g. It was concluded that, with buck percentages higher than 3%, the number of ejaculations of bucks is linearly and positively related to number of females in estrus. Also, these findings indicate that neither number of copulations nor number of services from different bucks affected kidding rates.     

Lack of sexual experience does not reduce the responses of LH, estrus or fertility in anestrous goats exposed to sexually active males

We investigated whether LH secretion, estrous behavior and fertility would differ between sexually inexperienced and experienced anestrous goats exposed to the males. Male goats were rendered sexually active during the reproductive rest season by exposure to 2.5 months of artificial long days. Two groups of anovulatory sexually inexperienced and sexually experienced does were exposed to males during 15 days (n = 20 per group). LH pulsatility was determined every 15 min from 4 h before to 8 h after introducing males (Day 0). Estrous behavior was recorded twice daily. Pregnancy rates were determined on Day 50. Fertility was determined at parturition. Male sexual behavior was registered on days 1 and 2 during 1 h. Before introducing the males, the number of LH pulses did not differ between groups. After introduction of the males, all females increased their LH pulsatility, but the number of pulses did not differ between sexually inexperienced and experienced goats. The proportion of females displaying estrous behavior with a high pregnancy rate and fertility did not differ between inexperienced and experienced goats. The sexual behavior of the males did not differ significantly between those interacting with sexually inexperienced or experienced goats. We conclude that goats can show substantial endocrine and reproductive responses to males, even in the absence of previous sexual experience, when sexually active bucks are used.     

No previous isolation of female goats is required for novel males to induce a male effect,especially if direct physical contact is established

Goat does supposedly need to be separated from bucks before male stimuli can induce reproductive activity, ovulation, and estrous. The present study examined the reproductive response (ovulation and estrous) of does to “novel” bucks in direct contact with them and when separated from them by a fence. One hundred fourteen does were distributed into three groups: (1) ISOL group (N = 31): females that had been completely isolated from males for 46 days, and thereafter placed in direct contact with “novel” males during the breeding period; (2) NOTISOL-CONTACT group (N = 29): females that had been in contact with “familiar” vasectomized males for 46 days and thereafter placed in direct contact with novel males during the breeding period; (3) NOTISOL-NO CONTACT group (N = 54): females that were in contact with familiar vasectomized males for 46 days and thereafter introduced to novel males, but separated by a fence, during the breeding period. All the males were treated with melatonin to ensure that they were sexually active. Estrous activity was recorded daily by direct visual observation of the marks left by marking harnesses worn by the males. Ovulation was confirmed via the plasma progesterone concentration (measured in weekly blood samples). The ovulation rate was assessed by transrectal ultrasonography. Fecundity, fertility, prolificacy, and productivity were also determined for the ISOL and NOTISOL-CONTACT groups (naturally, in the NOTISOL-NO CONTACT group, no pregnancies were possible). The introduction of novel males induced the same ovarian response in each group (P > 0.05), but the percentage of females that showed estrous and ovulation was lower in the NOTISOL-NO CONTACT group (P < 0.05). No differences were seen between the ISOL and NOTISOL-CONTACT groups (P > 0.05) in terms of fecundity, fertility, prolificacy, and productivity. These results show that the introduction of novel males to females already in contact with familiar males induces ovarian activity similar to that observed in the classical male effect (ISOL group) and that this response leads to a significantly higher estrus activity only when direct physical contact between the females and the novel males is established.     

Impact of cow strain and concentrate supplementation on grazing behaviour,milk yield and metabolic state of dairy cows in an organic pasture-based feeding system

As ruminants are able to digest fibre efficiently and assuming that competition for feed v. food use would intensify in the future, cereals and other field crops should primarily be destined to cover the dietary needs of humans and monogastric animals such as poultry and pigs. Farming systems with a reduced or absent concentrate supplementation, as postulated by organic agriculture associations, require adapted dairy cows. The aim of this experiment was to examine the impact of concentrate supplementation on milk production, grazing and rumination behaviour, feed intake, physical activity and blood traits with two Holstein-Friesian cow strains and to conclude the consequences for sustainable and organic farming. The experiment was a cross-over study and took place on an organic farm in Switzerland. In all, 12 Swiss Holstein-Friesian (HCH) cows and 12 New Zealand Holstein-Friesian (HNZ) cows, which were paired according to lactation number, days in milk and age for primiparous cows, were used. All cows grazed full time and were supplemented either with 6 kg/day of a commercial, organic cereal-grain mix or received no supplement. After an adaptation period of 21 days, a measurement period of 7 days followed, where milk yield and composition, pasture dry matter intake estimated with the n-alkane double-indicator technique, physical activity based on pedometer measurements, grazing behaviour recorded by automatic jaw movement recorder and blood samples were investigated. Non-supplemented cows had a lower milk yield and supplemented HCH cows produced more milk than supplemented HNZ cows. Grazing time and physical activity were greater for non-supplemented cows. Supplementation had no effect on rumination behaviour, but HNZ cows spent longer ruminating compared with HCH cows. Pasture dry matter intake decreased with the concentrate supplementation. Results of blood analysis did not indicate a strong negative energy balance for either non-supplemented or supplemented cows. Minor differences between cow strains in this short-term study indicated that both cow strains are equally suited for an organic pasture-based production system with no concentrate supplementation. Many factors such as milk yield potential, animal welfare and health, efficiency, grazing behaviour and social aspects influence the decision to supplement grazing dairy cows with concentrates.     

Positive correlation between the body weight of anestrous goats and their response to the male effect with sexually active bucks

In the present study, we analyzed the results of two years of response to the male effect in seasonally anestrous goats to investigate whether the activation of female reproductive activity by the male effect is related to the body weight of the females. Seventy-nine adult female Mexican mixed breed goats were used. The anestrous females were exposed during 15 days to sexually active males, and were classified into three categories according to their mean body weight +/-SD (42 +/- 9 kg) (Light: < or = 33 kg, n = 19; Medium: 34-50 kg, n = 46; Heavy: > or = 51 kg, n = 14). More than 98% of the goats from the Medium and Heavy groups showed at least one estrus behavior within the first 15 days following the introduction of the bucks, versus only 63% of the females from the Light group (P < 0.01). The interval between the introduction of the males and the onset of estrus behavior was longer in the females of the Light and Medium groups (4.2 +/- 0.8 and 3.3 +/- 0.3 days) than in the females of the Heavy group (2.0 +/- 0.2 days; P < 0.03). Also, body weight was negatively correlated with latency to first estrus (Spearman r = -0.57; P < 0.001). These results are in agreement with the hypothesis that the ability of anestrous goats to respond to the male effect is positively influenced by their body weight.     

Four hours of daily contact with sexually active males is sufficient to induce fertile ovulation in anestrous goats

The study was conducted on two consecutive years to determine whether ovulatory activity can be induced in anovulatory goats by exposing them to sexually active bucks for 4, 8, 12 or 16 h per day during 15 consecutive days. In experiment 1, females remained continuously in the experimental pens where they were in contact with males. One group remained isolated from males (controls) and four other groups were exposed to sexually active males for 4, 8, 12 or 16 h per day. In experiment 2, females were taken away to “resting” pens free of male odours between the periods of contact with bucks. They were allocated to 5 groups as in experiment 1. Ovulations were determined by progesterone plasma levels and transrectal ultrasonography. Pregnancy was determined by abdominal ultrasonography. In both experiments, more than 90% of females exposed to the bucks had at least one ovulation during the whole experiment whereas only 11 or 0% (experiments 1 and 2, respectively) did so in the control group (P < 0.001). Furthermore, the proportion of females ovulating did not differ among groups depending on duration of contact with bucks (P > 0.05). In both experiments, pregnancy rates were not affected by the daily duration of contact with males (P > 0.05). To conclude, 4 h of daily contact with sexually active males is sufficient to stimulate ovulatory activity in anovulatory goats and this effect is not due to the presence of olfactory cues from the males remaining in the pens.     

Influence of the type of energy supply on lh secretion, follicular growth and response to estrus synchronization treatment in feed-restricted suckler beef cows

The present experiment aimed to compare the efficiency of supplementation (+17.5 MJ Net Energy/d starting 47 +/- 4 days after calving) with concentrate (CS, maize grain, n = 10) or with forage (FS, maize silage, n = 10) in estrus-synchronized (Norgestomet implant 10 days inserted 60 +/- 4 days postpartum + PMSG at implant removal) beef cows previously restricted (47 MJ Net Energy/d, 785 g CP/d, 70% of requirements). The type of diet had no significant effect on basal LH concentrations (CS: 0.18 +/- 0.12 vs FS: 0.11+/- 0.02 ng/mL), LH pulse frequency (CS : 0.7 +/- 0.3 vs FS: 0.8 +/- 0.2 pulse/10 h), LH pulse amplitude (CS: 0.55 +/- 0.50 vs FS : 0.62 +/- 0.50 ng/mL) or estradiol (E2) concentrations (CS: 3.3 +/- 0.8 vs FS: 4.6+ /- 0.8 pg/mL) 13 days after the beginning of energy supplementation. No differences between CS and FS cows were observed for the number of small, medium and large follicles nor on the size of the largest follicle from 11 days before implant insertion to implant removal (IR). After IR, an LH surge was observed in 2 of the CS and 4 of the FS cows. The type of energy supplementation had no significant effect on LH (CS: 0.16 +/- 0.06 ng/mL vs FS 0.48 +/- 0.06 ng/mL; P > 0.05) or on estradiol concentrations (CS : 7.8 +/- 0.2 vs FS : 8.9 +/- 0.2 pg/mL, P > 0.10) measured hourly from 29 to 49 h after IR. Cows that ovulated after IR tended to have higher E2 concentrations than cows that did not ovulate (9.4 +/- 0.2 vs 6.3 +/- 0.2 pg/mL, P = 0.08). Similar ovulation and pregnancy rates were observed in CS and FS cows (CS: 6/10 vs FS: 7/10 and CS: 6/10 vs FS: 5/10 respectively, P > 0.05). To conclude, energy supplementation with forage was as effective as energy supplementation with concentrate to influence follicular growth, ovulation and pregnancy percentage after estrus synchronization treatment in diet-restricted beef cows.     

Effect of presence of the male on initiation of estrous cycle activity of goats

Serum progesterone concentrations and behavioral estrus were determined in two groups of 17 mixed breed dairy does at the beginning of the breeding season. The treatment group was pastured adjacent to two mature bucks while two teaser bucks ran with the group. The control group was pastured without exposure to bucks. Goats were observed for estrus daily for 35 days and samples of jugular blood were collected every other day for radioimmunoassay of progesterone. Signs of estrus were observed in 16 of 17 does in the treatment group within a mean +/- S.E. of 5.5 +/- 1.3 days after introduction of the bucks. Thirteen does demonstrated a progesterone profile characteristic of a normal estrous cycle with peak progesterone concentrations of 5.9 +/- 0.5 ng/ml. Signs of behavioral estrus were not observed in the control group. One control doe demonstrated a progesterone profile characteristic of a normal estrous cycle attaining a peak progesterone concentration of 3.9 ng/ml. Progesterone concentrations in the remaining 16 control does were at or near the lower limits of sensitivity of the assay for the duration of the experiment. Fifteen of the control does exhibited estrus within 7 +/- 1.5 days after exposure to bucks at the end of the experiment. These results clearly demonstrated a profound influence of the male on estrous cycle activity during the beginning of the breeding season.     

Reproduction of French Angora rabbits: ovulation in the female, semen production in the male]

Ovulation rate and semen quality in French Angora rabbits were investigated to determine the potential of improving breeding practices in this breed. The proportion of does ovulating and their ovulation rate were studied in 40 females, as well as sexual behaviour and semen quality in 8 males. The experiments took place in autumn 1987 and were repeated in winter. Three groups of does were injected with 25, 50 IU hCG or 0.8 microgram GnRH (groups 1, 2 and 3, respectively). Another group was mated and served as a control (group 4). Hormonal treatments improved the proportion of does which ovulated (95, 90, 74 and 28% in groups 1-4, respectively; P less than 0.05) but did not change their ovulation rate (10.9 +/- 0.7, 10.7 +/- 0.7, 11.3 +/- 0.8 and 8.9 +/- 1.3 corpora lutea per ovulating female, groups 1-4, respectively; m +/- SEM, NS). In males, two ejaculates were collected twice weekly for 3.5 weeks from 8 bucks. Ejaculates obtained in March were better than those collected in November (volume: 0.33 vs 0.23 ml, P less than 0.01; raw motility: 5.2 vs 3.9, P less than 0.01; individual motility: 2.7 vs 2.1, P less than 0.05; number of living spermatozoa per ejaculate: 71 vs 28 x 10(6), P less than 0.01, respectively). These results suggest that artificial insemination may be utilized to improve reproductive performance in French Angora rabbits.     

Rumen-protected choline and vitamin E supplementation in periparturient dairy goats: effects on milk production and folate, vitamin B12 and vitamin E status

We investigated the effects of rumen-protected choline (RPC) and vitamin E (VITE) administration on milk production and status of folate, vitamin B12 and vitamin E during the periparturient period of dairy goats. Forty-eight Saanen multiparous goats were selected for the 72-day experiment, being moved to a maternity pen 30 days before expected parturition and assigned to one of the four experimental groups: control (CTR), no choline or vitamin E supplementation; choline (RPC), supplemented with 4 g/day choline chloride in rumen-protected form; vitamin E (VITE), supplemented with 200 IU/day vitamin E in rumen-protected form; and choline and vitamin E (RPCE), supplemented with 4 g/day RPC chloride and 200 IU/day vitamin E. Supplements were administered individually before the morning feed to ensure complete consumption, starting 30 days before kidding and continuing for 35 days after. During the experiment, milk yield and 4% fat-corrected milk (FCM) yield were, respectively, 210 and 350 g/day higher in RPC-supplemented goats than in non-supplemented goats. Milk fat concentration and fat yield were also increased by RPC treatment. Milk yield and composition were unaffected by vitamin E supplementation. There were no significant interactions between RPC and VITE for any of the variables measured. Plasma metabolites did not differ between treatments before and after kidding except that plasma folate at parturition was higher in RPC-supplemented goats. Neither choline nor vitamin E affected vitamin B12 plasma concentrations, while a time effect was evident after the second week of lactation, when B12 levels in each treatment group started to increase. Vitamin E administration resulted in plasma α-tocopherol levels that were 2 to 2.5 times higher than in non-supplemented goats. Overall, these results suggest that greater choline availability can improve milk production and methyl group metabolism in transition dairy goats.     

Male reproductive condition is the limiting factor of efficiency in the male effect during seasonal anestrus in female goats

Two experiments were conducted to determine whether the failure of males to induce sexual activity in goats during seasonal anestrus is due to unresponsiveness of females to male stimulus or insufficient stimulation from males. In the first study, one group of males (sexually inactive, SI; n = 4) was kept under natural photoperiod while the other (sexually active, SA; n = 4) was subjected to 2.5 mo of long days (16L:8D) and received 2 s.c. implants of melatonin. Two mo later, 2 different flocks of anovulatory goats previously separated from bucks were exposed to either SI (n = 34) or SA (n = 40) bucks. Progesterone assays and estrous behavior were used to determine ovarian and behavioral responses of the females to teasing. Of the goats exposed to SI males, only 2 ovulated, and none showed estrous behavior during the 35 days of the study. In contrast, all females (40 of 40) in contact with SA males ovulated and showed at least one estrous behavior during the first 11 days following male introduction (P < 0.001). Overall, 38 of 40 females stimulated with SA bucks were diagnosed pregnant at Day 35, according to progesterone assay (versus 0 in SI-treated group: P < 0.001). To control for a possible difference of responsiveness between flocks, the experiment was repeated 1 yr later using a single flock of goats divided into 2 groups. Again, over the first 14 days, 1 of 33 goats showed estrous behavior in the SI-treated group versus 27 of 33 in the SA-treated group (P < 0.001). Therefore, treating bucks with long days and melatonin increased their teasing capacity to induce sexual activity in females during anestrus. These results indicate that the absence of response to teasing at this time of the year is not due to female unresponsiveness, but to insufficient stimulation from the male.     

Season determines timing of first ovulation in rhesus monkeys (Macaca mulatta) housed outdoors

In order to determine the relative importance of age and season on the occurrence of first ovulation in rhesus monkeys, the timing of puberty in spring-born females (Group S, N = 13) was compared to that of fall (N = 3) and winter-born (N = 5) females (Group W). All females were housed outdoors and were studied from 12 months of age through first ovulation. Menarche occurred at a similar age but significantly earlier in the year for Group W (31.2 +/- 0.7 months; 25 August +/- 19.5 days) than for Group S females (31.2 +/- 0.7 months; 14 November +/- 17.1 days). First ovulation, as assessed from twice weekly serum progesterone determinations, occurred exclusively in the fall or winter in a bimodal age distribution for all females. For Group W females, 6/8 ovulated during the 3rd year at 35.8 +/- 0.7 months while 2/8 ovulated during the 4th year at 45.3 +/- 0.1 months. In contrast, only 3/13 Group S females ovulated during the 3rd year and at a significantly younger age of 31.4 +/- 0.4 months compared to Group W. The remaining Group S females (10/13) ovulated the following autumn at 43.2 +/- 0.2 months, significantly younger than the later ovulating Group W females. In addition to this pattern of first ovulation, serum concentrations of prolactin varied seasonally, rather than with age, in both groups of females with higher levels in the summer and low levels in the winter.(ABSTRACT TRUNCATED AT 250 WORDS)     

Male influence on oestrous cycles in female woolly opossum (Caluromys philander)

Plasma progesterone concentrations and the occurrence of oestrous cycles were studied in isolated woolly opossums subsequently subjected to male influences during a 40-day period. Pairing (N = 48) or exposure to male urine (N = 15) resulted in all females exhibiting oestrous during the stimulation phase, providing evidence that the activation of ovarian activity in the woolly opossum involves pheromonal cues from males. The latency of occurrence of oestrous in stimulated females depended upon their sexual state before male stimulation. In anoestrous females, the mean latency was 20.7 +/- 0.9 days (N = 35), a value which agrees with the duration of the follicular phase. In females which first entered oestrous before male stimulation, the latency of induced oestrous was inversely correlated to the date of occurrence of the previous oestrous. The inter-oestrous interval was normal (38.1 +/- 1 days, N = 5) when females were in oestrous at the beginning of male stimulation. In contrast, the inter-oestrous interval was significantly shortened (28.7 +/- 2 days, N = 7) or lengthened (51.1 +/- 1.7 days, N = 16) depending on whether females were in the luteal or follicular phases at the beginning of male stimulation. During pairing several females became pregnant and gave birth 24 +/- 0.9 days (N = 13) after copulation. In the woolly opossum, the response to male influences involves mechanisms similar to those observed in eutherians and results in enhancement and synchronization of oestrous cycles in females. Pheromonal interactions could play an important role in synchronizing oestrous cycles in wild females during the dry season, a period when animals regroup to feed on spatially localized food resources.     

Effects of live yeast on the performance, nutrient digestibility, gastrointestinal microbiota and concentration of volatile fatty acids in weanling pigs

Two experiments were conducted to evaluate the effects of live yeast supplementation on performance, nutrient digestibility, enteric microbial populations and volatile fatty acid (VFA) concentration of weanling pigs, receiving diets supplemented with aureomycin and elevated doses of CuSO4. In experiment 1, 90 crossbred pigs (7.20 +/- 0.44 kg, 28 d of age) were randomly allotted to one of five dietary treatments containing either 0, 4.0 x 10(6), 9.0 x 10(6), 2.6 x 10(7), or 5.1 x 10(7) cfu Saccharomyces cerevisiae per gram with six replicate pens per treatment and three pigs per pen. BWG and feed intake increased quadratically during days 1-14 and days 1-28 as live yeast levels increased (p < 0.01). Pigs fed the diet containing 2.6 x 10(7) cfu yeast per gram had the highest BWG and feed intake among the treatments. In experiment 2, 48 crossbred pigs (7.64 +/- 0.72 kg, 28 d of age) were fed diets containing live yeast at 0 or 3.2 x 10(7) cfu of S. cerevisiae per g with six replicate pens per treatment and four pigs per pen. The yeast supplementation improved BWG and feed intake during days 1-14 (p < 0.01) and days 1-28 (p < 0.05). Treatment differences were not observed in any of the bacterial populations, yeast numbers or VFA concentrations, at any of the sites of the gastrointestinal tract tested. Total tract nutrient digestibility was also not different between treatments. Overall, dietary supplementation of live yeast had a positive effect on BWG and feed intake of weanling pigs, receiving diets supplemented with aureomycin and elevated doses of CuSO4. The improvement in BWG appears to be partly related to an increase in feed intake. The mechanism of yeast improving feed intake of piglets needs to be explored.