Longidorus grandis n. sp. and L. paralongicaudatus n. sp. (Nematoda: Longidoridae), Two Parthenogenetic Species from Arkansas

Two new parthenogenetic species of Longidorus were found in Arkansas. Longidorus grandis n. sp. is characterized by its body (5.80-8.24 mm), slightly offset head, head width 20-27 µm, odontostyle 86-100 µm, guide ring 26-35 µm posterior to the anterior end, short conoid to mammiliform tail. Longidorus grandis n. sp. is similar to L. vineacola Sturhan &Weischer, 1964; L. lusitanicus Macara, 1985; L. edmundsi Hunt &Siddiqi, 1977; L. kuiperi Brinkman, Loof &Barbez, 1987; L. balticus Brzeski, Peneva &Brown, 2000; L. closelongatus Stoyanov, 1964; and L. seinhorsti Peneva, Loof &Brown, 1998. Longidorus paralongicaudatus n. sp. is characterized by its body length (2.60-5.00 µm), anteriorly flattened and offset head region 13-18 µm wide, odontostyle length 92-127 µm, guide ring 21-30 µm posterior to the anterior end, tail elongate-conical, and c'' = 1.2-2.6. Longidorus paralongicaudatus n. sp. most closely resembles L. longicaudatus Siddiqi, 1962; L. socialis Singh &Khan, 1996; L. juvenilis Dalmasso, 1969; and L. curvatus Khan, 1986.     

Morphological and Molecular Characterization of Longidorus americanum n. sp. (Nematoda: Longidoridae), a Needle Nematode Parasitizing Pine in Georgia

We describe and illustrate a new needle nematode, Longidorus americanum n. sp., associated with patches of severely stunted and chlorotic loblolly pine, (Pinus taeda L.) seedlings in seedbeds at the Flint River Nursery (Byromville, GA). It is characterized by having females with a body length of 5.4-9.0 mm; lip region slightly swollen, anteriorly flattened, giving the anterior end a truncate appearance; long odontostyle (124-165 µm); vulva at 44%-52% of body length; and tail conoid, bluntly rounded to almost hemispherical. Males are rare but present, and in general shorter than females. The new species is morphologically similar to L. biformis, L. paravineacola, L. saginus, and L. tarjani but differs from these species either by the body, odontostyle and total stylet length, or by head and tail shape. Sequence data from the D2-D3 region of the 28S rDNA distinguishes this new species from other Longidorus species. Phylogenetic relationships of Longidorus americanum n. sp. with other longidorids based on analysis of this DNA fragment are presented. Additional information regarding the distribution of this species within the region is required.     

Description of Pratylenchoides camachoi n. sp. (Tylenchida: Pratylenchidae) from Spain

Pratylenchoides camachoi n. sp. from rosemary (Rosmarinus officinalis L.) in Sierra De Cazorla in southeastern Spain is described and illustrated. Pratylenchoides camachoi n. sp. is a bisexual species characterized by a cylindroid, sometimes clavate-shaped, male tail with a uniquely shaped smooth, rounded terminus. Females are characterized by a short esophageal overlap and six lines in the lateral field, which reduce to four in the tail region; not areolated except outer bands in the esophageal and tail regions.     

Marine Nematodes of Bothnian Bay,Finland: Ethmolaimus hailuotoensis n. sp. (Ethmolaimidae: Nemata)

Ethmolaimus hailuotoensis n. sp. from Bothnian Bay, northern Baltic Sea, is described and illustrated. Ethmolaimus hailuotoensis n. sp. has a strongly sclerotized luminal lining of the terminal esophageal bulb and esophagus. The most characteristic feature is a lateral series of large concentric, sclerotized rings with a fine, internal longitudinal oval sclerotization. In addition, E. hailuotoensis n. sp. is distinguished from other Ethmolaimus species by its smaller body size, fewer number of precloacal supplements (7-8), conical tail, and the position of amphid posterior to the buccal cavity. Females of E. hailuotoensis n. sp. studied were hermaphroditic. Ethmolaimus hailuotoensis n. sp. coexisted with E. pratensis. Ethmolaimus pratensis from Bothnian Bay has a characteristic row of sublateral hypodermal glands, a character not before seen in Ethmolaimidae.     

Three New Species of Nothacrobeles (Nemata: Cephalobidae) from the Mojave Desert, California

Three new species of Nothacrobeles are described from localities in the Mojave Desert, southern California. Nothacrobeles triniglarus n. sp. is characterized by the presence of a long post-vulval sac and three tubular adoral projections. Both N. spatulatus n. sp. and N. nanocorpus n. sp. are smaller than any other known species within the genus. Nothacrobeles spatulatus n. sp. has labial probolae that are short and spatulate without a basal ridge, whereas those of N. nanocorpus n. sp. are flattened and plate-like. Furthermore, N. nanocorpus n. sp. is unique by its extremely short esophageal corpus (less than 25 µm long in adult females) and the small size of its guard processes. An emended diagnosis of the genus is given to accommodate distinctive characteristics of these new species. A table comparing the 11 valid species of Nothacrobeles is presented.     

Heterorhabditidoides rugaoensis n. sp. (Rhabditida: Rhabditidae), a Novel Highly Pathogenic Entomopathogenic Nematode Member of Rhabditidae

A novel entomopathogenic nematode species, Heterorhabditidoides rugaoensis n. sp. RG081015, collected from Rugao, China, is described. The new species is morphologically very similar to H. chongmingensis but can be distinguished from it on the basis of some morphological characteristics, combined with molecular data and a cross-hybridization test. Males of the new species can be recognized on the basis of body length averaging 1396.2 μm; lateral field with one ridge; metastome isoglottoid with one hemispherical swellings comprised of two to three well-developed warts; asymmetric spicules; peloderan bursa. In IJs, EP = 134.5 μm; ES = 149.3 μm; tail length = 82.5 μm; and a = 20.5. Hermaphroditic females have four to five lateral ridges. The 18S rDNA and ITS sequences of the two nematodes share 99% and 98% identity, respectively. Phylogenetic trees of 18S rDNA and ITS indicate that the new species is most closely related to H. chongmingensis; thus, the two nematodes belong to the same genus. Failure of cross-hybridization between them indicates that nematode strain RG081015 is a novel species and is described herein as H. rugaoensis n. sp. The LC₅₀ of the novel species against Galleria mellonella were 24.35 IJs / ml within 48 hours of infection. Morphological characteristics, genetic similarity analyses, and phylogenetic relationships provide strong evidence that some species of Oscheius/Insectivora-group should be reassigned to the genus Heterorhabditidoides.     

Description of Bakernema dauniense n. sp. (Nematoda: Criconematidae) from Italy

Bakernema dauniense n. sp., a bisexual species from the rhizosphere of Pinus halepensis Mill. in Italy, is described and illustrated. Primary differentiating characteristics of the female are body annules bearing short, membranous projections 1.4-2.2 μm long, an anterior vulval lip slightly overlapping the posterior, and a linearly arcuate vagina. The juvenile differs markedly from adults by having annules with a beaded margin, which lack membranous projections. Generic and specific relationships of B. dauniense n. sp. are discussed. This occurrence is the first for a species of Bakernema in Europe.     

Three New Species of Brevitobrilus (Nematoda) with a Discussion on Relationships within the Genus

Three new species of the genus BrevitobriusTsalolikhin, 1981 are described. Brevitobrilus glandulatus n. sp. is characterized by conspicuous sphincter between pars dilatata and uterus; two pairs of vaginal glands; spicules having elliptical capitula with small proximal stiffening piece; proximally-arcuate gubernaculum; S3 and S4 smaller than other supplements; S6 out of spicular range and 57-60 micropapillae. Brevitobrilus dimorphicus n. sp. is diagnosed by sexual dimorphism in labial sensilla and amphids; thick-walled rectum with a diverticulum protruding into intestinal lumen and males with boat-shaped spicules and S6 occasionally slightly smaller than other supplements. Brevitobrilus allahabadensis n. sp. possesses large amphids of 28-33% of corresponding labial diameter in both sexes; vagina and uterus with muscular, plicate walls; well developed sphincter between vas deferens and ejaculatory duct; capitulate spicules with sloping ventral and angular dorsal walls; S3, S4 and S6 smaller than other supplements, S6 close to cloaca and 28-37 micropapillae. The relationships of the species of genus Brevitobrilus have been assessed using morphological characters subjected to parsimony and a non cladistic key to identification of species is given.     

Description of Afenestrata koreana n. sp. (Nematoda: Heteroderinae), a Parasite, of Bamboo in Korea

Afenestrata koreana n. sp. collected from roots of bamboo (Phyllostachys pubescens) in Gyeongnam Province in the southern part of the Korean peninsula is described and illustrated. Its primary differentiating characteristics are a globose to subspherical body in adults with a prominent neck and terminal cone, thick cuticle, terminal vulva, and deep vagina. Fenestra, bullae, and underbridge are absent. The anus is on the immediate posterior side of the cone. Superficial small tubercules cover all the terminal cone area. The new species differs markedly from the other two known species in the genus, specifically in having three incisures in the lateral field of juveniles and a shorter stylet length in juveniles and adults. The male is unknown.     

Longidorus ferrisi n. sp. from California Citrus

In October 1999, the authors received fixed specimens of a species of Longidorus from Howard Ferris found about the roots of a citrus tree in Oakville, Napa County, CA. After determining it to be new a species, we requested additional specimens. The samples contained roughly equal numbers of males and females. Longidorus ferrisi n. sp. is most similar to L. elongatus, but can be distinguished by a greater c-ratio (111-187 vs 73-141), a lesser c′ (0.7-1.1 vs 1.0-1.3), a more offset head, a more posterior guide ring (35-40 vs 30-33 μm), the presence of sperm in the uterus in mature females, and the approximate 1:1 ratio of females to males. Other similar species include L. artemisiae, L. crassus, L. glycines, and L. milanis. Longidorus ferrisi n. sp. differs from L. artemisiae by a lesser a-ratio (74-102 vs 109-155), a lesser c′ value (0.7-1.1 vs 1.0-1.6), a more posterior guide ring (35-40 vs 27-34 μm), a longer odontostyle (91-108 vs 84-98 μm), a wider lip region (16-19 vs 14-17 μm), wider mid-body (53-69 vs 41-52 μm), and longer spicules (57-65 vs 39-49 μm). The new species differs substantially from L. crassus by its lip shape and the presence of males, and differs from L. glycines by a shorter body (4.33-5.97 vs 6.14-8.31 mm), a lesser c′ value (0.7-1.1 vs 0.9-1.4), a narrower lip region (16-19 vs 20-23 μm), wider mid-body (53-69 vs 39-57 μm), longer spicules (53-69 vs 45-53 μm), and fewer supplements (7-11 vs 11-17). Longidorus ferrisi n. sp. differs from L. milanis by a longer body (4.33-5.97vs 3.00-4.90 mm), a greater c value (111-187 vs 86-130), a wider mid-body (53-69 vs 43-56 μm), a different head shape, and longer spicules (53-69 vs 41-54 μm). The nuclear 18S ribosomal DNA sequence of this species revealed that this species is unique with respect to all sequenced Longidorus species.     

Onychiurid species from Wanda Mountains in China,with descriptions of two new species (Collembola,Onychiuridae)

A checklist of onychiurid species from the Wanda Mountains in China is presented. Eighteen species belonging to twelve genera have been found, including two new species. Bionychiurus qinglongensissp. n. can be easily distinguished from other known species of the genus by the absence of pseudocelli on Th. I tergum and fewer number of vesicles in postantennal organ. Onychiurus heilongjiangensissp. n. is diagnosed by pseudocellar formulae as 32/133/33352 dorsally and 3/011/31120 ventrally, parapseudocellar formula as 0/000/111001+1^m, ratio of anal spine/unguis as 0.6, unguiculus without inner basal lamella, and male ventral organ absent.     

Review of Chinese Oligaphorurini (Collembola, Onychiuridae) with descriptions of two new Palaearctic species

A checklist of Chinese Oligaphorurini is given. Two new Chinese species, Micraphorura changbaiensissp. n. and Oligaphorura pseudomontanasp. n., are described from Changbai Mountain Range. Micraphorura changbaiensis sp. n. has the same dorsal pseudocelli formula and number of papillae in Ant. III sensory organ as Micraphorura uralica, but they can be easily distinguished by number of chaetae in Ant. III sensory organ, ventral pseudocelli formula, ventral parapseudocelli formula, number of pseudocelli on subcoxa 1 of legs I-III, dorsal axial chaeta on Abd. V and number of chaetae on tibiotarsi. Oligaphorura pseudomontana sp. n. is very similar to the species Oligaphorura montana having an increased number of pseudocelli on body dorsally, well marked base of antenna with 1 pseudocellus and 3 pseudocelli outside, subcoxa 1 of legs I-III with 1 pseudocellus each, dorsally S-chaetae formula as 11/011/22211 from head to Abd. V, S-microchaeta present on Th. II-III, claw without inner teeth and with 1+1 lateral teeth, and unguiculus with basal lamella; but they can be separated easily by the number of pseudocelli on Abd. V and VI terga, parapseudocelli on the body, number of chaetae on Th. I tergum, and number of chaetae on tibiotarsi. A key to Chinese species of Oligaphorurini is provided in the present paper.     

Meloidogyne morocciensis n. sp. (Meloidogyninae), a Root-knot Nematode from Morocco

Meloidogyne morocciensis n. sp. is described from specimens parasitic on peach rootstock from Morocco. This species exhibits a combination of morphological characters similar to M. arenaria, M. incognita, and M. javanica. The perineal pattern of females is oval to squarish with a moderately high to high dorsal arch, and widely spaced, smooth striae; lateral lines are absent. The stylet, 16.5 μm long, has transversely ovoid, set-off knobs. Males have a set-off, annulated head region. The large, rounded labial disc is distinctly demarcated from the crescent-shaped medial lips; lateral lips are absent. The robust stylet, 24.6 μm long, has large, rounded knobs that taper slightly posteriorly. Mean second-stage juvenile (J2) length is 401 μm. The set-offhead region has incomplete annulations; the lip structures are dumbbell shaped. The stylet, 12.3 μm long, has rounded knobs that slope posteriorly. The J2 tail, 52.6 μm long, has irregularly sized annules in the posterior region and ends in a bluntly rounded tip. Tomato, tobacco, pepper, and watermelon are good hosts; cotton and peanut are not hosts. Meloidogyne morocciensis n. sp. reproduces by mitotic parthenogenesis and has a somatic chromosome number of 47-49. Its esterase phenotype is identical with the three-banded phenotype (A3) of M. arenaria.