共查询到20条相似文献,搜索用时 15 毫秒
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Jie Wu Sol Kim Man Sup Kwak Jang Bin Jeong Hyun Jin Min Ho-Geun Yoon Jin-Hyun Ahn Jeon-Soo Shin 《The Journal of biological chemistry》2014,289(29):20000-20011
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The mechanisms of PML-nuclear body formation 总被引:1,自引:0,他引:1
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Lin X Sun B Liang M Liang YY Gast A Hildebrand J Brunicardi FC Melchior F Feng XH 《Molecular cell》2003,11(5):1389-1396
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Jakobs A Koehnke J Himstedt F Funk M Korn B Gaestel M Niedenthal R 《Nature methods》2007,4(3):245-250
Although small ubiquitin-like modifier (SUMO) is conjugated to proteins involved in diverse cellular processes, the functional analysis of SUMOylated proteins is often hampered by low levels of specific SUMOylated proteins in the cell. Here we describe a SUMO-conjugating enzyme (Ubc9) fusion-directed SUMOylation (UFDS) system, which allows efficient and selective in vivo SUMOylation of proteins. Although SUMOylation of overexpressed p53 and STAT1 was difficult to detect in HEK293 cells, up to 40% of p53 and STAT1 were conjugated with endogenous SUMO when fused to Ubc9. We verified the specificity of UFDS using SUMOylation-site mutants and showed that the method is not dependent on SUMO ligases. Using UFDS we demonstrated that SUMOylation of STAT1 inhibits its phosphorylation at Tyr701 and discovered p53 multi-SUMOylation in vivo. We propose that UFDS will be useful for the analysis of function of SUMOylation in protein interactions, subcellular localization as well as enzymatic activity. 相似文献
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