原子荧光和电感耦合等离子体质谱法在硒形态分析中的应用

硒是人体必需的微量元素之一,具有抗肿瘤、防衰老、防辐射和增强机体免疫力等多种功能。近年来,随着人们生活水平的不断提高,各种各样的富硒产品及富硒保健品走进了我们的生活,硒的形态测定越来越受到人们广泛的关注。本文对目前主要检测硒形态的两种方法—原子荧光法(AFS)和电感耦合等离子质体谱法(ICP-MS)在食品、水果蔬菜、富硒保健品、生物样品等方面的应用进行了综述。     

富硒酵母中硒赋态的研究

硒是人体必需的一种微量元素,参与合成硒代半胱氨酸、硒代甲硫氨酸以及多种硒代蛋白(酶),具有抗肿瘤、抗氧化、增强人体免疫等多种生物学活性,与人体的健康有着密切关系.硒以不同的形式存在于自然界中,大致可分为无机硒和有机硒两种,其生物活性与毒性也各有不同.富硒酵母作为补充硒元素的主要形式之一,具有生物利用度高、食用安全、毒性低等优点.研究富硒酵母中的硒的赋态,对合理摄取硒元素,促进人体健康具有重要意义,因此成为近年来研究的热点.     

富硒产品中硒的形态分析及化学评分模式的建立

采用微波水解、HPLC-HG-AFS法测定了硒蛋白粉、硒蛋白片、肽粉、富硒原料等19种硒产品中的总硒、硒代氨基酸和亚硒酸根离子[Se(IV)],分析了硒代氨基酸、Se(IV)和其他形态硒占总硒的百分比及不同形态硒代氨基酸的组成比例。以此为依据,将硒产品分为硒蛋白型、单一硒代氨基酸型、其它形态硒型及有机无机硒混合型。根据DBS42/002-2014规定建立了富有机硒产品评分模式,其中18种为富有机硒产品;根据适硒地区母乳中硒代氨基酸的组成比例提出了硒代氨基酸的化学评分模式,评分结果显示13种以蛋白态硒为主的硒产品中硒代氨基酸的组成比例均与母乳相差甚远,不利于人体平衡吸收利用,其中10种SeMet含量远远超过人体所需,SeCys_2为限制硒代氨基酸。该评分模式的建立对硒产品的开发具有指导意义。     

恩施州天然硒资源特征及其开发利用研究进展

硒是人和动物所必需的微量元素,也是恩施的优势特色资源。从恩施州的硒资源特征、开发利用情况以及对硒研究的问题和建议两方面,探讨了恩施硒资源的研究现状,并对其未来对硒资源的开发利用方向进行了展望,以期为后续的硒相关研究以及富硒产品的产业开发提供参考和指导。     

富硒食品的研究进展与展望

介绍了富硒食品中硒元素的优点,总结了富硒产品的分类、国内外富硒食品的研究现状以及硒元素的检测方法,并对富硒食品的前景进行了展望。     

中国典型高硒区硒的环境地球化学研究进展

硒是人体和动物必需的微量元素,但其营养性的阈值范围较窄,已引起了不同领域研究者的广泛关注。综述了近20年来我国典型高硒区——湖北恩施富硒岩石与土壤中硒的分布与形态、硒的赋存状态、硒的迁移转化规律、硒的生物可利用性与人体健康风险、微生物与硒相互作用等硒的环境地球化学过程与循环规律,以及硒的同位素地球化学等方面的重要研究进展,并对硒的环境地球化学未来的研究方向进行了展望。     

灵芝富硒研究进展

对灵芝的富硒和耐硒特性,硒对灵芝生长代谢的影响,富硒灵芝中硒的赋存形态和含量分布以及富硒灵芝药理活性等方面的研究进展作了概述,并对灵芝富硒研究的发展趋势和前景进行了探讨。     

富硒益生菌的功效研究进展

硒是人体必需的微量元素,对人体健康有重要作用。益生菌能够将硒元素转化为有机硒,降低硒的毒性,同时硒又提高了益生菌的生物活性,富硒益生菌具备了硒和益生菌的双重功效。本文主要综述了近年来富硒益生菌的功效,如抗氧化、抑制有害菌、调节肠道菌群、抗癌等。     

基于生态位理论的富硒土壤资源开发利用适宜性评价及分区方法

实施乡村振兴战略,产业兴旺是关键。立足区域富硒土壤资源禀赋优势,发展富硒农业特色经济,是振兴乡村产业的一条有效途径。以多维超体积生态位理论为指导,以区域富硒土壤资源禀赋和富硒农业发展要求为基础,从土壤自身条件、土地利用和生态保护3个方面明确了富硒土壤资源开发利用的主导因素、区位因素、限制因素;选择土壤硒含量、土壤有机质含量、地形坡度、土地利用类型、土地综合污染指数、生态保护建设等6个关键因子,构建了富硒土壤资源开发利用生态位适宜性评价指标体系;利用生态位适宜性评价相关模型,探讨了生态位适宜性单一指数和综合指数的计算方法;结合土壤利用改良分区、土壤利用功能分区、富硒农业生产分区等,分析了区域富硒土壤资源开发利用适宜性分区问题。研究可为富硒土壤资源开发利用适宜性评价提供科学可行的思路与方法,对于促进区域富硒土壤资源科学合理利用、实现富硒农业产业科学发展具有重要的指导意义和现实意义。     

富硒食用菌的研究进展

富硒食用菌是一类有价值的药用/功能性食品.从食用菌的富硒特性、富硒食用菌的营养成分、药理作用及生产技术等方面进行了总结.     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.