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1.
ELLIS R. H.; COVELL S.; ROBERTS E. H.; SUMMERFIELD R. J. 《Journal of experimental botany》1986,37(10):1503-1515
Positive linear relationships were shown between constant temperaturesand the rates of progress of germination to different percentiles,G, for single populations of each of five genotypes of chickpea(Cicer anetinum L.). The base temperature, Tb, at which therate of germination is zero, was 0·0°C for all germinationpercentiles of all genotypes. The optimum temperature, To(G),at which rate of germination is most rapid, varied between thefive genotypes and also between percentiles within at leastone population. Over the sub-optimal temperature range, i.e.from Tb to To(G), the distribution of thermal times within eachpopulation was normal. Consequently a single equation was appliedto describe the influence of sub-optimal temperatures on rateof germination of all seeds within each population of each genotype.The precision with which optimum temperature, Tb(G), could bedefined varied between populations. In each of three genotypesthere was a negative linear relationship between temperatureabove Tb(G) and rate of germination. For all seeds within anyof these three populations thermal time at supra-optimal temperatureswas constant. Variation in the time taken to germinate at supra-optimaltemperatures was a consequence of normal variation in the ceilingtemperature, To(G)the temperature at or above which rateof progress to germination percentile G is zero. A new approachto defining the response of seed germination rate to temperatureis proposed for use in germplasm screening programmes. In two populations final percentage germination was influencedby temperature. The optimum constant temperature for maximumfinal germination was between 10°C and 15°C in thesepopulations; approximately 15°C cooler than the optimumtemperature for rate of germination. It is suggested that laboratorytests of chickpea germination should be carried out at temperaturesbetween 10°C and 15°C. Key words: Chickpea, seed germination rate, temperature 相似文献
2.
The Effects of Priming and Ageing on Seed Vigour in Tomato 总被引:1,自引:0,他引:1
A comparison was made of the effects of seed priming or ageingtreatments on the performance of tomato (Lycopersicon esculentumMill. cv. UC204C) seeds according to a number of indices ofseed vigour. A single lot of tomato seeds was primed in 120mol m3 K2HPO4 + 150 mol m3 KNO3 for 5 d at 20?C, or aged at 13% moisture content (dry weight basis) and 50?C for 6 d. Germination percentage (>98%) was unaffectedby priming and reduced to 85% by ageing. X-ray photographs andlongitudinal sections revealed the formation of free space surroundingthe embryo in dry primed seeds, which was not evident in controlor aged seeds. Priming increased the rate of germination atall temperatures above the base temperature (Tb), while ageingdecreased it. Tb was unaffected by priming and only slightlyincreased by ageing. The variation in individual times to germinationwas approximately doubled in both primed and aged seed comparedto the control, based upon the slopes of probit germinationpercentage versus log thermal time curves. Root growth aftergermination tests and seedling growth in both greenhouse andfield tests were not influenced by either priming or ageing.The conductivity test was found to be unreliable as a vigourtest for tomato seeds. The results identify several indiceswhich can be used to quantify seed vigour in tomato. They alsoillustrate that seed priming can enhance seed performance accordingto some criteria, while having no effect or decreasing qualityaccording to other criteria. Seed vigour can apparently be separatedinto various components which can be independently influencedby seed enhancement treatments. Key words: Tomato, seed germination rate, seed priming, seed vigour 相似文献
3.
Effects of Priming and Endosperm Integrity on Seed Germination Rates of Tomato Genotypes: I. GERMINATION AT SUBOPTIMAL TEMPERATURE 总被引:2,自引:0,他引:2
DAHAL PEETAMBAR; BRADFORD KENT J.; JONES RICHARD A. 《Journal of experimental botany》1990,41(11):1431-1439
The bases of differences in germination rates (GRg = inverseof time to germination [tg] of percentage g) among three cold/salt-toleranttomato (Lycopersicon esculentum Mill.) accessions (PI 341988,PI 120256, and PI 174263) and one cold/salt-sensitive tomatocultivar (T5) were investigated. The effects of seed priming(6 d imbibition in aerated 1.2 MPa polyethylene glycolsolution at 20 ?C followed by redrying) and of removing theendosperm/testa cap covering the radicle on the temperaturesensitivity of GRg, and the interaction of these treatmentswith genotypes, were also examined. GRg decreased linearly withdecreasing temperature for all genotypes and seed treatments.The minimum or base temperatures for germination (Tb) variedby 1 ?C among the tomato lines, so genotypic differences inGRg were due to differing thermal time requirements for germination.The mean thermal time requirement for germination of T5 seeds was 22% and 19% greater than that of PI 341988 andPI 120256 seeds, respectively, but only 9% greater than thatof PI 174263 seeds. Seed priming did not lower Tb of any genotype,but significantly reduced by 24, 49, 41, and 49% in T5, PI 341988, PI 120256, and PI 174263, respectively,indicating that priming increased the rate at which the seedsprogressed towards germination when T>Tb, but did not lowerthe minimum temperature at which germination could occur. Primingincreased the GRg of T5 seeds to equal or exceed those of control(non-primed) seeds of the cold/salt-tolerant genotypes at anyT>Tb, but the PI lines exhibited an even greater responseto priming. Times to germination within each seed lot were normallydistributed on a logarithmic scale. Priming increased the variancein tg within a seed lot when compared to control seeds. However,the variation in thermal time for germination between the 10thand 90th percentiles of the seed population (T(1090))was relatively unaffected by priming due to the reduction in in primed seeds. Removing the endosperm cap and testa opposite the radicle tip decreased almost 6-fold and and reduced Tb by 5 ?C in T5 and PI 341988,implicating processes in the endosperm/testa as the limitingfactors in germination at suboptimal temperatures. Key words: Lycopersicon esculentum Mill., tomato, genetic variation, seed priming, thermal time, germination rate 相似文献
4.
The role of temperature in the seed germination of two species of the Solanum nigrum complex 总被引:2,自引:0,他引:2
The germination behaviour of S. nigrum L. and S. physalifoliumRusby var. nitidibacatum (Bitter) Edmonds is compared, basedon temperature requirements during imbibition. Three seed lotsof S. nigrum had base temperatures (Tb) between 7.5C and 10C,showing a lower Tb when the period of freezing days, duringwhich each population was collected, was reduced. S. physalifoliumhas a higher value for Tb at constant temperatures (21C) thatcan be interpreted as a dormancy constraint. This constraintis released by alternating temperatures at amplitudes exceeding5C and with the high temperature above 21C by apparently reducingTb to 12.5C. This implies that for S. physaiifolium temperature has a dualeffect on germination. It is the driving force for changes indormancy, but germination also depends on the temperature. Therole of temperature for S. nigrum is simpler: each populationcollected showed differences in the thermal time required forgermination that could be related to the temperature regimenof the original environment. Key words: Dormancy, S. nigrum, S. physalifolium, thermal time 相似文献
5.
Ellis, R. H., Simon, G. and Covell, S. 1987. The influence oftemperature on seed germination rate in grain legumes. III.A comparison of five faba bean genotypes at constant temperaturesusing a new screening method.J. exp. Bot. 38: 10331043. A screening procedure which requires information on the progressof germination at only four temperatures was able to definethe response of the rate of seed germination to sub- and supra-optimaltemperatures for whole seed populations of each of five fababean (Vicia faba L.) genotypes. In one population of the cultivarSutton the models for sub- and supra-optimal temperatures derivedfrom the screen satisfactorily explained observations from anearlier separate investigation at a wider range of temperatures.Two discrete groups of genotypes were identified. Within eachgroup the base temperature Tb did not differ significantly:for the landraces Lebanese Local Large and Syrian Local Largethe value was estimated to be 7·5°C and forthe landrace Lebanese Local Small and the cultivars Sutton andAquadulce it was 4·0°C. The optimum temperaturefor the 50th percentile [To(50), at which temperature the rateof germination is maximal] also varied between these two groupsof genotypes, being 20·521·5°C forthe first group and 24·526·0°C forthe second. In several temperature regimes some of the viableseeds within a seed population failed to germinate. Nevertheless,even at temperatures where a substantial proportion of the seedsfailed to germinate the models defined by the screening methodpredicted the germination times of those seeds which did germinate. Key words: Faba bean, seed gemination rate, temperature 相似文献
6.
Effects of Temperature and Water Potential on Germination, Radicle Elongation and Emergence of Mungbean 总被引:2,自引:1,他引:1
Controlled environment experiments were performed to determinethe effects of temperature and water potential on germination,radicle elongation and emergence of mungbean (Vigna radiata(L.) Wilczek cv. IPB-M79-17-79). The effects of a range of constant temperatures (1545°C) and water potentials (0 to 2.2 MPa) on germinationand radicle elongation rates were studied using an osmoticumtechnique, in which seeds were held against a semi-permeablemembrane sac containing a polyethylene glycol solution. Linearrelationships were established between median germination time(Gt50) and water potential at different temperatures, and betweenreciprocal Gt50 (germination rate) and temperature at differentwater potentials. Germination occurred at potentials as lowas 2.2 MPa at favourable temperatures (3040 °C),but was fastest at 40 °C when water was not limiting, withan estimated base temperature (Tb) of about 10 °C. Subsequentradicle elongation, however, was restricted to a slightly narrowertemperature range and was fastest at 35 °C. The conceptof thermal time was used to develop an equation to model thecombined effects of water potential and temperature on germination.Predictions made using this model were compared with the actualgermination obtained in a related series of experiments in columnsof soil. Some differences observed suggested the additionalimportance of the seed/soil/water contact zone in influencingseed germination in soil. Seedling emergence appeared to reflectfurther the radicle elongation results by occurring within anarrower range of temperatures and water potentials than germination.Emergence had an estimated Tb of 12.6 °C and was fastestat 35 °C. A soil matric potential of not less than about0.5 MPa at sowing was required to obtain 50% or moreseedling emergence. Key words: Germination, temperature, water potential 相似文献
7.
Common bean (Phaseolus vulgaris L.) is grown over a wide rangeof environments, including sites with low or high soil temperaturesat sowing time. To describe the temperature responseof seedgermination, 20 bean genotypes were evaluated using a rolledpaper towel system with 11 constant temperatures ranging from12 to 34 °C. Germination response was characterized by fittingcumulative counts using a maximum-likelihood analysis. Rateof germination increased from abase temperature (Tb) typicallynear 8 °C to an optimal development temperature (To) of29 to 34 °C. Tb did not differ among common bean genotypes.Mesoamerican germplasm showed slightlyhigher To than Andeangermplasm, but there was large variation in To within each ofthe two gene pools. The single accession of tepary bean (P.acutifolius) evaluated appeared to be the mosttolerant to highgermination temperatures. Key words: Common bean, seed germination rate, temperature 相似文献
8.
The onset of germination of faba bean seeds at constant temperaturewas progressively delayed as that temperature diverged froman optimum of 25.5 ?C. At temperatures below 10 ?C, or above28 ?C, the maximum germination percentage fell to below 90%.There was no germination at 39 ?C. Positive and negative linearrelationships were established between the constant temperaturesand the rates of progress of germination to different percentiles,at sub-optimal and supra-optimal temperatures, respectively.Like germination rates, base temperature (Tb) declined from3.71 to 0.83 ?C as the percentile value increased from10% to 80%. Caution was urged in extrapolating beyond the experimentaldata set. Differences in the ceiling temperature (Tc) with percentilecould not be discerned. Cumulative germination progress curves at each temperature weremodelled by the Weibull, logistic, and cumulative normal distributionfunctions. Cardinal temperatures (Tb and Tc) calculated fromthese data reasonably approximated the actual data. The Weibullfunction demonstrated a good approximation at all percentilelevels, while the logistic and cumulative normal distributionfunctions, as a result of their inherent symmetry, deviatedat the extreme percentiles. It was concluded that the Weibullfunction not only accurately modelled cumulative germinationbut could also be used in the calculation of cardinal temperatures. Key words: Seed germination rate, cardinal temperatures, faba bean, Weibull function, probit and logic scales 相似文献
9.
Alternating Temperatures and Rate of Seed Germination in Lentil 总被引:2,自引:2,他引:0
The effect of alternating temperatures on the times taken byseeds of lentil (Lens culinaris Medikus) to germinate was investigatedusing a two-way temperature-gradient plate. Between 5 and 25°C,warmer temperatures increased the rate of germination. Variationamong the individual seeds in the times required for germinationat different constant temperatures within this range were describedwell by a log-normal distribution of thermal times, accumulatedabove a base temperature of 1·5°C. Even with amplitudesas great as 20°C, no effect of alternation per se on thethermal time required for germination was detectedwhetherthe cool temperature was applied for 8 or 16 h d-1. Similarly,in alternating temperature regimes where the minimum temperatureof the diurnal cycle was between 0°C and the base temperature,the thermal times required for germination (where no thermaltime accrued during the periods when temperature was below Tb)were in close agreement with those values provided by the modeldetermined at warmer constant temperatures. However, where theminimum temperature applied was < 0°C the germinationof all but the earliest germinators was delayed beyond modelpredictions, and more so where the sub-zero minimum temperaturewas applied for 16 rather than 8 h d-1. The results, therefore,contradict the view that alternation in temperature per se reducesthe thermal time required for seed germination. Rather, rateof germination responds instantaneously to current temperature,but prolonged exposure to sub-zero temperatures can result indamage sufficient to delay germination when seeds are returnedto regimes warmer than the base temperature.Copyright 1994,1999 Academic Press Lens culinaris Medikus, lentil, seed germination, alternating temperatures, thermal time, temperature-gradient plate 相似文献
10.
Seed priming (imbibition in water or osmotic solutions followedby redrying) generally accelerates germination rates upon subsequentre-imbibition, but the response to priming treatments can varyboth within and among seed lots. Seed maturity could influenceresponsiveness to priming, perhaps explaining variable primingeffects among developmentally heterogeneous seed lots. In thecurrent study, muskmelon (Cucumis melo L.) seeds at two stagesof development, maturing (40 d after anthesis (DAA)) and fullymature (60 DAA), were primed in 0?3 M KNO3 for 48 h at 30 ?C,dried, and imbibed in polyethylene glycol 8000 solutions of0 to 1?2 MPa at 15, 20, 25, and 30 ?C. Germination sensitivitiesto temperature and water potential () were quantified as indicatorsof the influence of seed maturity and priming on seed vigour.Germination percentages of 40 and 60 DAA control seeds weresimilar in water at 30 ?C, but the mean germination rate (inverseof time to germination) of 40 DAA seeds was 50% less than thatof 60 DAA seeds. Germination percentages and rates of both 40and 60 DAA seeds decreased at temperatures below 25 ?C. Reductionsin also delayed and inhibited germination, with the 40 DAAseeds being more sensitive to low than the 60 DAA seeds. Primingsignificantly improved the performance of 40 DAA seeds at lowtemperatures and reduced , but had less effect on 60 DAA seeds.Priming lowered both the minimum temperature (Tb) and the minimum (b) at which germination occurred. Overall, priming of 40 DAAseeds improved their germination performance under stress conditionsto equal or exceed that of control 60 DAA seeds, while 60 DAAseeds exhibited only modest improvements due to priming. Asthe osmotic environment inside mature fruits approximates thatof a priming solution, muskmelon seeds may be primedin situ during the late stage of development after maximum dryweight accumulation. Key words: Cucumis melo L., seed priming, germination, vigour, development, temperature 相似文献
11.
Hydrothermal time analysis of tomato seed germination responses to priming treatments 总被引:5,自引:1,他引:4
Controlled hydration of seeds followed by drying (seed priming) is used to
break dormancy, speed germination, and improve uniformity of radicle
emergence. To date, empirical trials are used to predict optimal priming
conditions for a given seed lot. Since priming is based upon seed water
relations, it was hypothesized that the sensitivity of germination to
reduced water potential before priming might be mechanistically related to,
and therefore predictive of, priming responsiveness. Analyses of
germination of 13 tomato (Lycopersicon esculentum
Mill.) seed lots at two temperatures (15C
and 20C) and three water potentials (0,
-0.28 and -0.43 MPa) showed that seed lot germination responses could be
quantitatively characterized by parameters derived from thermal time,
hydrotime, and hydrothermal time models
(R20.73-0.99). Six of the seed
lots were primed at two temperatures (15°C and 20°C) and
three water potentials (-1.0, -1.5 and -2.0 MPa) for various durations,
dried, and their subsequent germination rates analysed according to
hydropriming time and hydrothermal priming time models. The responses of
germination rates to priming were characterized by hydropriming time
(HP) and hydrothermal priming time (HTP) constants and
the minimum water potential (min) and temperature
(Tmin) for achieving a priming effect. The values of
min and Tmin varied relatively little among
tomato seed lots, and the generalized values of min=2.39 MPa and
Tmin=9.10°C accounted for 74% (15°C),
57% (20°C), and 62% (across both temperatures) of the increase in
germination rates following priming. Nonetheless, while the hydrothermal
time models described germination patterns both before and after priming,
there was relatively little predictive relationship between
them. 相似文献
12.
The Influence of Temperature and Soil Water Deficit on the Development and Morphology of Groundnut (Arachis hypogaea L.) 总被引:2,自引:0,他引:2
The rate/temperature relation of several developmental processesin groundnut was examined in a suite of temperature-controlledglasshouses maintained at mean air temperatures of 19, 22, 25,28 and 31 °C. The sensitivity of the various processes tosoil water deficit was also examined. When the relation between rate and temperature was linear, measurementswere analysed in terms of thermal time (°Cd) and an extrapolatedbase temperature (Tb) at which the rate was zero. Tb was conservative(10 °C) for leaf appearance, branching, flowering, peggingand podding. A higher value of Tb for seedling emergence (16°C) was probably an artifact caused by soil pathogens. Leafappearance and branching were more sensitive to soil water deficitthan the other processes examined. Key words: Temperature, Soil water deficit, Development, Groundnut 相似文献
13.
Using flow cytometric analyses of the nuclear DNA content, westudied the effects of various conditions of osmopriming onthe activation of the cell cycle in embryo root tips of tomato(Lycopersicon esculentumElko) seeds. In dry untreatedseeds, 90.7% of the nuclei revealed 2C signals. Priming of seedsin polyethylene glycol-8000 (PEG) improved the germination rateof seeds transferred onto water at 15 °C. This was associatedwith an increase in 4C signals when priming was carried outat -1.0 and -1.5 MPa. Priming at -2.0 MPa enhanced subsequentgermination but had no effect on DNA replication. For temperaturesduring priming up to 25 °C, a positive linear correlationexisted between the efficiency of the treatment, evaluated bythe reciprocal of time to obtain 50% germination at 15 °C,and the frequency of 4C nuclei or the 4C/2C values. Such a correlationdid not exist when priming was performed at higher temperatures.At least 5% oxygen in the atmosphere was required during primingfor the induction of DNA replication and for the enhancementof subsequent germination. In the presence of 5x10-4M and 10-3MNaN3during priming, most of the cells were maintained with 2CDNA levels and the treatment had no stimulatory effect on germination.The results show a positive linear relationship between thefrequency of 4C DNA nuclei or the 4C/2C ratio and the improvingeffect of priming. However, in suboptimal conditions of priming(-2.0 MPa or temperatures higher than 25 °C), the improvementof seed germination was not associated with the onset of DNAreplication.Copyright 1999 Annals of Botany Company Cell cycle, germination, osmopriming, oxygen, temperature, Lycopersicon esculentum, tomato. 相似文献
14.
Dormant and after-ripened seeds of Agrostemma githago (corn-cockle)were pretreated in polyethylene glycol 400 (PEG) solutions attemperatures which would have allowed germination if the seedshad been imbibed in water, viz. 4?C or 20?C for after-ripenedseeds, and 4?C for dormant seeds. Pretreated seeds germinatedfaster than untreated seeds. The maximum decrease of the T50(time to 50% germination) was 66%. Furthermore, pretreated seedswere capable of germination at supra-optimal temperatures whichotherwise had inhibited germination completely (20?C for dormantseeds and 30?C for after-ripened seeds). The percentage germinationat a supra-optimal temperature was considerably higher whenthe seeds had been primed at a temperature at which they developedmore extension power. The advantageous effects of the osmotic pretreatment were lessthan might be expected when the osmoticum had inhibited onlycell elongation. This was largely, if not fully, due to a generaldetrimental effect of osmotic stress and not to a selectiveinhibition of the processes which occur during the pregerminativephase in preparation for growth. Thus, during priming seedscomplete all or almost all processes which occur in water-imbibedseeds prior to radicle emergence. Key words: Agroatemma githago, dormancy, germination, germination performance, osmotic stress, priming 相似文献
15.
SYNOPSIS. Terrestrial and semi-terrestrial crustaceans are exposedto fluctuations in ambient temperature and conditions that favorevaporative water loss. These environmental stresses alter performancelimits in the laboratory and behavior in the field. The maximalrate of oxygen consumption, maximum aerobic speed, and endurancecapacity are greater at a body temperature (Tb) of 24°Cthan at 15°C or 30°C in the ghost crab, Ocypode quadrata.The total metabolic cost to move at the same relative speedis greater at a Tb of 24°C than at 15°C. Slower aerobickinetics at 15°C result in a smaller relative contributionof oxidative metabolism to total metabolic cost. However, therelative contributions from accelerated glycolysis are similarat both temperatures. When locomotion is intermittent, the totaldistance traveled before fatigue can be similar at Tbs of 15and 24°C but result from different movement and pause durationsat these temperatures. Performance limits of the ghost crabare negatively affected by dehydration and are sensitive torates of water loss. In the laboratory, endurance capacity ofthe fiddler crab, Uca pugilator, is greater at a Tb of 30°Cthan at 25°C. In the field, freely moving fiddler crabswith a Tb of 30°C travel at faster mean preferred speeds,as determined by motion analysis, than crabs at 25°C. Datafor land crabs support and advance general ectothermic modelsfor the effects of temperature and dehydration on locomotorperformance. 相似文献
16.
The germination of pearl millet (Pennisetum typhoides S. &H.) seeds was investigated at constant temperatures between12 ?C and 47 ?C on a thermal gradient plate. The rate of germination increased linearly with temperaturefrom a base Tb to a sharply defined optimum To beyond whichthe rate decreased linearly with temperature, reaching zeroat Tm. The linearity of the response both above and below Toallowed time and temperature to be combined in a thermal timeat which a specified fraction of the seeds germinated. Withinthe population Tb and Tm were constant. 相似文献
17.
The intact dormant seeds of Dioscorea tokoro germinate slowlyif at all between 11-23°C; for full and rapid germinationthey require prior chilling treatment [Okagami and Kawai (1982)Bot. Mag. Tokyo 95: 155]. The germination abilities of zygoticembryos detached from dormant seeds of this species were studiedunder various nutritional and temperature regimes. For germinationof embryos, the minimum nutritional components in Murashigeand Skoog's (1962) medium that were required were sucrose andNO3 or SO24. As the source of carbohydrate forgermination of detached embryos, sucrose, mannose and maltosewere effective; glucose and fructose were less effective; andrhamnose was entirely unable to support germination. Embryos detached from dormant seeds, incubated with the sucroseplus KNO3, germinated more rapidly with increasing temperatureup to 35°C. However, application of sucrose and KNO3 didnot induce germination of intact seeds above 26°C. Therefore,it is very possible that the endosperm exerts an inhibitoryfunction on germination at such high temperatures. When seeds were incubated after a cut was made over a smallpart of the edge of the endosperm in which the radicle of theembryo is encased, germination occurred rapidly but the increasein germination percentage was slight. This result suggests thatthe endosperm suppots part of the germination inhibition bymeans of a mechanical barrier or its impermeability to wateror gases. Physiological features of the endosperm alone or interactionsbetween the embryo and endosperm may contribute significantlyto the characteristics of dormancy of intact seeds of this species. (Received May 30, 1988; Accepted January 11, 1989) 相似文献
18.
Seeds of two lettuce cultivars (Lactuca sativa L., cv. Meikoninginand cv. Grand Rapids) were hermetically stored with constantmoisture contents ranging between 3.6 and 17.9 per cent (freshweight basis) at constant temperatures ranging between 5 and75 °C. The decline with time in percentage germination andpercentage normal seedlings was determined for each storagetreatment. The data were fitted to an equation which containsthe constants: K1, the probit of the initial percentage germinationor normal seedlings; KE, a species constant; CW, the constantof a logarithmic moisture term; CH, the constant of a lineartemperature term and CQ, the constant of a quadratic temperatureterm. Regression analysis of data from storage periods up to5.5 years at temperatures of 575 °C and seed moisturecontents of 3.613.6 per cent yielded the following values:KE= 8.218, CW=4.797±0.163, CH=0.0489±0.0050 andCQ=0.000365±0.000056. Although this equation consistentlyprovided a better fit, simplified equations, assuming eithera log-linear relationship between seed longevity and temperature,or a log-linear relationship between seed longevity and bothmoisture content and temperature, accounted for more than 94per cent of the variation at the restricted temperature rangeof 540 °C. Longevity of the same seed lots at sub-zero temperatures (5,10 and 20 °C) was studied in separate tests.Freezing damage, resulting in abnormal seedlings in the germinationtest, occurred at 20 °C when the moisture contentof the seeds exceeded 12 per cent. No decline in percentagenormal seedlings was observed after a storage period of 18 monthsor longer at 20 °C, provided the seed moisture contentdid not exceed 9.5 per cent. For seeds stored at 5 and10 °C with 9.612.5 per cent moisture content,the observed rate of decline of percentage normal seedlingswas adequately predicted by the viability equation, using theabove values for the constants. This suggests that for low moisturecontents the viability equation can be applied to estimate longevityat sub-zero temperatures. Lettuce, Lactuca sativa (L.), seed longevity, seed storage, viability constants, storage conditions 相似文献
19.
A linear relationship between constant temperatures in the sub-optimaltemperature range and germination rate is shown in both Quercusrobur L and Castanea sativa Mill germinated under nominal darkconditions. The mean base temperature was interpolated for Qrobur as 0 8 ? or 2-4 ?, depending on seed lot provenance, andfor C. sativa as 1 -4? The optimum temperature for germinationin Q. robur was about 20? compared with around 28 ? in C. sativaOver the sub-optimal temperature range the distribution of thermaltimes was log-normal for each population studied their spreadvarying both between Q robur seed lots and between species However,in C. sativa germinated close to the mean base temperature,the distribution in thermal times was reduced Thermal timesto germination were decreased in Q. robur and C sativa by approximately0 3 and 0-5 log-units, respectively, when the pericarp was removed,i.e in the seeds, but the sensitivity of the response remainedrelatively unaltered In both species the germination rate was the same when nominaldark or safe green light conditions were employed during thegermination test. However, at 21 ? Q robur exhibited the highirradiance reaction (HIR) at photon doses above 30mmol m2d1. HIR first affected the germination rate by an inhibitionof radicle extension The sensitivity of the response to thermaltime was reduced as photon dose increased. This photo-inhibitionwas exacerbated at supra-optimal temperatures. In contrast,C. sativa germination rate at 26 ? was little influenced bylight at a photon dose of 752 mmol m2 d1 Key words: Seed germination rate, temperature, thermal time, light, photon dose 相似文献
20.
COVELL S.; ELLIS R. H.; ROBERTS E. H.; SUMMERFIELD R. J. 《Journal of experimental botany》1986,37(5):705-715
For a single seed population of each of four species of grainlegume positive linear relationships were shown between temperatureand rate of germination for different fractions (G) of eachpopulation, from a base temperature, Tb(G), at which germinationrate is zero, to an optimum temperature, To(G) at which germinationrate is maximal. At constant temperatures warmer than To(G)there were negative relations (probably linear) between temperatureand rate of germination to the maximum temperature for germination,Tm(G), Within each population Tb(G) did not differ, but it didvary between species, viz.0.0?C, 0.25?C, 4.and 8.5?C for chickpea(Cicer arietinum L.), lentil (Lens culinaris Medic.), soyabean(Glycine max [ Merr.) and cowpea (Vigna unguiculata [L.] Walp.),respectively. In contrast, To(G) varied both within each populationand also between the four species: 80% of seeds in each populationhad To(G) values within the range 31.8?C to 33.8 ?C, 24.0?Cto 24.4?C, 34.0?C to 34.5?C and 33.2?C to >40?C, respectively.Values of Tm(G) were much more vanable: the 80% population rangewas 48 .0?C to 60.8?C for chickpea, 31.8?C to 34.4?C for lentiland 46.8?C to 55.2?C for soyabean; reliable estimates couldnot be made for cowpea, but the results suggest higher and morevariable values of Tm(G) than in the other three species. Atsub-optimal temperatures the distribution of thermal time forthe different fractions of each population was normal, exceptfor lentil where it was log-normal. A single equation is proposedto describe the influence of sub-optimal temperatures on ratesof germination for whole seed populations. At supra-optimaltemperatures, variation in thermal time for the different fractionsof each population was only slight. The implications of thesefindings for the adaptation of grain legume crops to differentenvironments, and for the screening of germplasm, are discussed. Key words: Seed germination rate, temperature, grain legumes 相似文献