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1.
Alternating Temperatures and Rate of Seed Germination in Lentil   总被引:2,自引:2,他引:0  
Ellis  R. H.; Barrett  S. 《Annals of botany》1994,74(5):519-524
The effect of alternating temperatures on the times taken byseeds of lentil (Lens culinaris Medikus) to germinate was investigatedusing a two-way temperature-gradient plate. Between 5 and 25°C,warmer temperatures increased the rate of germination. Variationamong the individual seeds in the times required for germinationat different constant temperatures within this range were describedwell by a log-normal distribution of thermal times, accumulatedabove a base temperature of 1·5°C. Even with amplitudesas great as 20°C, no effect of alternation per se on thethermal time required for germination was detected—whetherthe cool temperature was applied for 8 or 16 h d-1. Similarly,in alternating temperature regimes where the minimum temperatureof the diurnal cycle was between 0°C and the base temperature,the thermal times required for germination (where no thermaltime accrued during the periods when temperature was below Tb)were in close agreement with those values provided by the modeldetermined at warmer constant temperatures. However, where theminimum temperature applied was < 0°C the germinationof all but the earliest germinators was delayed beyond modelpredictions, and more so where the sub-zero minimum temperaturewas applied for 16 rather than 8 h d-1. The results, therefore,contradict the view that alternation in temperature per se reducesthe thermal time required for seed germination. Rather, rateof germination responds instantaneously to current temperature,but prolonged exposure to sub-zero temperatures can result indamage sufficient to delay germination when seeds are returnedto regimes warmer than the base temperature.Copyright 1994,1999 Academic Press Lens culinaris Medikus, lentil, seed germination, alternating temperatures, thermal time, temperature-gradient plate  相似文献   

2.
Light and temperature control of germination in Agropyron smithii seeds   总被引:2,自引:0,他引:2  
In darkness, A. smithii seeds germinated poorly at constanttemperatures but well at alternating temperatures. Prolongedperiods on the high part of the temperature cycles reduced germination;the higher the temperature the shorter was the period requiredon the high part of the temperature cycles for optimum germination.Continuous, unfiltered, incandescent illumination and intermittentfar red at 15?–25?C alternation also inhibited germination;the inhibitory effects were similar to those caused by the highintensity reaction. Far red inhibited germination when appliedafter 1 and 2 complete 15?–25?C cycles in darkness butnot after 3 cycles. Less than 20% of the seeds were under phytochromecontrol at constant 20?C. When red light was applied directlyafter far red that was applied in intermittent cycles at 15?–25?C,however, 50% of the seeds caused to germinate by the alternatingtemperature were shown to be controlled by the reversible phytochromereaction. The induced high-temperature dormancy was overcome by gibberellicacid (GA3) plus kinetin. The hormonal treatment was much moreeffective than light for breaking dormancy. Inhibition fromprolonged illumination was alleviated or eliminated by GA3+kinetin.The failure of red light to promote good germination at 20?Cwas also overcome with GA3+kinetin; effects of light plus thehormone treatments were more than additive. These data suggestthat optimum alternating temperatures facilitate a proper balanceand interaction of hormones, enzymes, substrates and possiblypreexistent Pfr so that the germination of A. smithii seedscan proceed without benefit of a light treatment. (Received July 7, 1976; )  相似文献   

3.
Seeds of the obligate parasitic plants, Orobanche spp., wereconditioned in water or GA3for 2 or 12 weeks and then stimulatedto germinate by the synthetic stimulant GR24. Temperature treatmentsduring the germination tests comprised 169 different constantand alternating temperature regimes on a two-dimensional gradientplate. Optimum temperatures for germination of seeds of O. aegyptiacaand O. crenata were 18–21 °C and 18 °C, respectively.However, longer conditioning periods slightly lowered the optimain both species, and the maximum germination percentage wasalso reduced due to an induction of secondary dormancy. At agiven mean temperature, more seeds germinated at constant thanat alternating temperatures. Results were analysed in termsof characteristics of alternating temperatures that appearedto control germination, i.e. mean temperature, maximum temperature,amplitude (difference between daily maximum and minimum temperatures)and thermoperiod (the time spent at the maximum temperatureeach day). Final germination was modelled on the basis of therebeing two prerequisites for germination: a minimum mean temperaturewhich must be exceeded and a maximum temperature above whichthe seed will not germinate. These two requirements were assumedto be independent and to be normally distributed in the seedpopulation so that final germination could be described by amultiplicative probability model. Because of the response tomaximum temperature, inhibitory effects were more evident atalternating temperatures. Amplitude and thermoperiod influencedthis effect of maximum temperature. The implications of thedetrimental effect of alternating temperatures for germinationofOrobanche spp. in the field are discussed. Copyright 1999Annals of Botany Company Orobanche aegyptiaca, O. crenata, O. cernua, O. minor, broomrape, seed germination, temperature, germination model, secondary dormancy.  相似文献   

4.
A Model for Germination Responses to Alternating Temperatures   总被引:4,自引:2,他引:2  
When seeds of Chenopodium album are imbibed in 0–01 Mpotassium nitrate solution in the light at constant temperature,percentage germination increases to an optimum at 24 °C,above which it decreases. These relationships are linear ifpercentage germination values are transformed to normal deviates.At supra-optimal temperatures, alternating temperatures havelittle or no effect on this basic relationship. However, atsub-optimal temperatures normal deviate germination increaseslinearly with amplitude at constant mean temperature and theincreases are relatively greater at lower mean temperatures.The effect of amplitude is also greater when more time is spentat the wanner temperature in the diurnal cycle. Seeds of Panicummaximum show very similar responses except that the effect ofamplitude is greater when a shorter time is spent at the warmertemperature in the diurnal cycle. These observations form thebasis of a quantitative model which, at sub- and supra-optimaltemperatures, respectively, accounts for 90 and 75% of the variationin germination of C. album seeds subject to a very wide rangeof thermal environments on a two-dimensional temperature gradientplate. The data presented for P. maximum are less comprehensive,but again the model accounts for 80% of the variation. The relevanceof the same model to two unrelated species from different climatesand of different ecological behaviour suggests that it may begenerally useful in determining optimum temperatures for seedviability testing regimes and ultimately for predicting fieldbehaviour These results allow the producers to improve the productionof homogeneous populations of cyclamen seedlings Chenopodium album L., Panicum maximum Jacq., seed dormancy, germination, alternating temperatures, temperature gradient plate  相似文献   

5.
Probert, R. J., Gajjar, K. H. and Haslam, I. K. 1987. The interactiveeffects of phytochrome, nitrate and thiourea on the germinationresponse to alternating temperatures in seeds of Ranunculussceleratus L.: A quantal approach.—J. exp. Bot. 38: 1012–1025. The interactive effects of phytochrome, potassium nitrate andthiourea on the germination response to alternating temperaturesin achenes (seeds) of Ranunculus sceleratus L. were studied.Using thermogradient bars, high levels of germination were recordedover a broad range of alternating temperatures providing seedsreceived daily irradiations. Reduced germination in temperaturecycles with a relatively long warm phase was related to thelevel of the active form of phytochrome (Pfr). Dose-responseexperiments to red light (R) and temperature shifts showed thatthe actions of Pfr and alternating temperatures were interdependent.Maximum germination was recorded when intermittent pulses ofR were combined with daily 4 h temperature shifts from 16°Cto 26°C. Whilst probit analysis showed that potassium nitrateand thiourea both increased population sensitivity to temperatureshifts, thiourea was a more potent stimulant. Although the effectof both chemicals was dependent on phytochrome photo-equilibriumthe threshold level of Pfr required for thiourea action wasclearly much lower than that required for nitrate action. Thioureapotentiated a response to daily temperature shifts even whenPfr was at a low, normally inhibitory level. These results indicatedifferent mechanisms of action for potassium nitrate and thioureain relation to phytochrome controlled seed germination. Key words: Phytochrome, nitrate, thiourea, alternating temperatures, germination  相似文献   

6.
From a single population of achenes (seeds) of Ranunculus sceleratusL. sub-populations were selected on the basis of the sensitivityof individuals to an increasing number of daily light/temperatureshift cycles. Each cycle comprised a 4 h pulse of red lightfollowed by a 4 h temperature shift from 16 to 31 ?C. Selectionfor low dormancy (NND and ND) and high dormancy (D and DD) populationsresulted in a > 5-fold difference in the number of cyclesrequired for 50% germination. Despite a shift in the mean levelof dormancy the distributions of sensitivity (slopes of dose-responsecurves) were similar in all four selected populations. Differentialeffects of cold stratification on the germination response tolight and alternating temperatures were related to the depthof primary dormancy. The proportion of individuals that respondedpositively to the dormancy breaking effects of cold stratificationfollowed the trend DD < D < ND < NND. In high dormancypopulations (D and DD) the rate and uniformity of germinationof some individuals was reduced by cold stratification, indicatinga dormancy inducing effect. Over the range 2 to 11 ?C the effectivenessof dormancy release or dormancy induction was inversely relatedto temperature. The effects of cold stratification on the expressionof dormancy in R. sceleratus are discussed in relation to areproductive strategy involving winter and summer annual behaviour. Key words: Cold stratification, selection, dormancy, light, alternating temperatures, germination  相似文献   

7.
Germination of spinach (Spinacia oleracea L. var. grabra cv.Nobel) seeds was inhibited at a high temperature (35?C). Effectsof KCN on the respiration of seeds incubated at 20 and 35?Cwere compared in order to investigate the mechanism of inhibitionof seed germination by high temperature. Respiration of germinatingseeds incubated at 20?C was inhibited about 50% by 5 mM. KCNsolution, whereas it hardly inhibited the weak respiration ofthe seeds at 35?C. Germination of seeds was delayed by exogenousKCN. When the KCN solution was renewed daily, germination wascompletely inhibited. Pericarp removal promoted germinationat 35?C, but atypical germination (cotyledons emerging earlierthan a radicle) took up more than half of the total germination.The inhibitory action of KCN on the respiration of seeds wasnot altered by pericarp removal. A KCN addition, even at 20?C,elicited atypical germination in the pericarp-less seeds. Theseresults show that cyanide-sensitive respiration is needed toinduce typical spinach seed germination (root emergence), butis rendered inoperative by high temperatures thus bringing aboutpoor germination and atypical germination. (Received December 1, 1984; Accepted February 8, 1985)  相似文献   

8.
Stotzky , G., and Elsie A. Cox . (Central Research Labs., United Fruit Co., Norwood, Mass.) Seed germination studies in Musa. II. Alternating temperature requirement for the germination of Musa balbisiana. Amer. Jour. Bot. 49(7): 763–770. Illus. 1962.—Alternating temperatures were found to be required for the germination of seeds of Musa balbisiana. The temperature differentials optimal for germination in soil are dependent upon both the high and low temperatures, and range from 8–23 C. Germination is maximal when the seeds are held 6–12 hr at the high (27–35 C) and 12–18 hr at the low (12–18 C) temperatures. Some germination can be induced by short exposures to alternating temperatures followed by constant high temperatures, but continuous exposure to alternating temperatures is necessary for maximum germination. Excised embryos develop better at constant than at alternating temperatures, showing that the mechanisms affected by alternating temperatures reside elsewhere in the seed. Alternating temperatures are also required for germination of mechanically scarified seeds, although the temperature differentials are less than those necessary for intact seeds, indicating that the action of alternating temperatures is not on the permeability of the integuments.  相似文献   

9.
The germination of Lycopus europaeus seeds depends absolutelyon exposure to light and fluctuating temperatures. Studies oftemperature responses were made to establish the minimum fluctuationrequired for a response, the interaction of temperature andexposure time in different parts of the alternating temperaturecycle, and the effects of successive transfers between cyclingtemperature conditions. There was a complex interaction betweenthese three. The minimum fluctuation never fell below 6.5 °Cbut varied up to c. 15 °C according to other test conditions.High temperatures favoured rapid responses, and exposure totemperatures above 20 °C in one or other phase of the temperaturecycle was essential for a full response. No response occurredeither at any temperature under constant conditions, or if onlyone temperature change was given. Under some conditions a singlecycle of alternating temperatures, including two changes oftemperature, promoted high germination rates.  相似文献   

10.
Dormant and after-ripened seeds of Agrostemma githago (corn-cockle)were pretreated in polyethylene glycol 400 (PEG) solutions attemperatures which would have allowed germination if the seedshad been imbibed in water, viz. 4?C or 20?C for after-ripenedseeds, and 4?C for dormant seeds. Pretreated seeds germinatedfaster than untreated seeds. The maximum decrease of the T50(time to 50% germination) was 66%. Furthermore, pretreated seedswere capable of germination at supra-optimal temperatures whichotherwise had inhibited germination completely (20?C for dormantseeds and 30?C for after-ripened seeds). The percentage germinationat a supra-optimal temperature was considerably higher whenthe seeds had been primed at a temperature at which they developedmore extension power. The advantageous effects of the osmotic pretreatment were lessthan might be expected when the osmoticum had inhibited onlycell elongation. This was largely, if not fully, due to a generaldetrimental effect of osmotic stress and not to a selectiveinhibition of the processes which occur during the pregerminativephase in preparation for growth. Thus, during priming seedscomplete all or almost all processes which occur in water-imbibedseeds prior to radicle emergence. Key words: Agroatemma githago, dormancy, germination, germination performance, osmotic stress, priming  相似文献   

11.
Freshly-harvested and dried seeds of Bromus sterilis L. withrespectively 52-54% and less than 10% moisture contents, differedmarkedly in their rates of germination and in their responsesto light. Dried seeds attained full germination in darknessat 15 ?C by 4 d from sowing and were inhibited by the formationof Pfr. In contrast, freshly-harvested seeds required 3-4 weeksto attain maximum germination and were promoted by Pfr. Themodified photoresponse of the seeds was induced only when theseeds were dried in an alternating temperature regime. The resultsare discussed in relation to the importance of desiccation asthe trigger for the transition from the developmental to thegerminative and growth phases of seed maturation of Bromus steriliswhich is well-documented in seeds of other species. Key words: Bromus sterilis, desiccation, light  相似文献   

12.
The germination of pearl millet (Pennisetum typhoides S. &H.) seeds was investigated at constant temperatures between12 ?C and 47 ?C on a thermal gradient plate. The rate of germination increased linearly with temperaturefrom a base Tb to a sharply defined optimum To beyond whichthe rate decreased linearly with temperature, reaching zeroat Tm. The linearity of the response both above and below Toallowed time and temperature to be combined in a thermal timeat which a specified fraction of the seeds germinated. Withinthe population Tb and Tm were constant.  相似文献   

13.
Seed priming (imbibition in water or osmotic solutions followedby redrying) generally accelerates germination rates upon subsequentre-imbibition, but the response to priming treatments can varyboth within and among seed lots. Seed maturity could influenceresponsiveness to priming, perhaps explaining variable primingeffects among developmentally heterogeneous seed lots. In thecurrent study, muskmelon (Cucumis melo L.) seeds at two stagesof development, maturing (40 d after anthesis (DAA)) and fullymature (60 DAA), were primed in 0?3 M KNO3 for 48 h at 30 ?C,dried, and imbibed in polyethylene glycol 8000 solutions of0 to –1?2 MPa at 15, 20, 25, and 30 ?C. Germination sensitivitiesto temperature and water potential () were quantified as indicatorsof the influence of seed maturity and priming on seed vigour.Germination percentages of 40 and 60 DAA control seeds weresimilar in water at 30 ?C, but the mean germination rate (inverseof time to germination) of 40 DAA seeds was 50% less than thatof 60 DAA seeds. Germination percentages and rates of both 40and 60 DAA seeds decreased at temperatures below 25 ?C. Reductionsin also delayed and inhibited germination, with the 40 DAAseeds being more sensitive to low than the 60 DAA seeds. Primingsignificantly improved the performance of 40 DAA seeds at lowtemperatures and reduced , but had less effect on 60 DAA seeds.Priming lowered both the minimum temperature (Tb) and the minimum (b) at which germination occurred. Overall, priming of 40 DAAseeds improved their germination performance under stress conditionsto equal or exceed that of control 60 DAA seeds, while 60 DAAseeds exhibited only modest improvements due to priming. Asthe osmotic environment inside mature fruits approximates thatof a priming solution, muskmelon seeds may be ‘primed’in situ during the late stage of development after maximum dryweight accumulation. Key words: Cucumis melo L., seed priming, germination, vigour, development, temperature  相似文献   

14.
A break on the Arrhenius plot of germination activity in rice seeds   总被引:1,自引:0,他引:1  
The germination activity of rice seeds was estimated at a numberof different temperatures for varieties including both japonicaand indica types. A break was found on the Arrhenius plot ofgermination activity. The breaking temperature was approximately17?C, irrespective of the variety or the germination activityat 25?C. (Received November 21, 1974; )  相似文献   

15.
The germination of cassava seed in response to various constantand alternating temperature regimes within the range 19–40°C was investigated using a two-dimensional temperaturegradient plate. It was found that almost all seeds were incapableof germination unless the temperature for part of the day exceeded30 °C and the mean temperature was at least 24 °C. However,dormant seeds required environments where the temperature forpart of the day exceeded 36 °C, the mean temperature wasat least 33 °C, and the amplitude of the diurnal temperaturealteration was within the range 3–18 °C. Providingthese conditions were met, the times spent at the upper andlower temperatures within a diurnal cycle were not critical.Hermetic storage of the seed for 77 days at 40 °C with 7.9per cent moisture content did not influence the pattern of germinationin response to constant and alternating temperatures. It issuggested that an alternating temperature regime of 30 °Cfor 8 h/38 °C for 16 h applied for a minimum of 21 daysis appropriate for cassava seed viability tests. Manihot esculenta Crantz, cassava, germination, dormancy, temperature  相似文献   

16.
Use of the Weibull Function to Calculate Cardinal Temperatures in Faba Bean   总被引:1,自引:0,他引:1  
The onset of germination of faba bean seeds at constant temperaturewas progressively delayed as that temperature diverged froman optimum of 25.5 ?C. At temperatures below 10 ?C, or above28 ?C, the maximum germination percentage fell to below 90%.There was no germination at 39 ?C. Positive and negative linearrelationships were established between the constant temperaturesand the rates of progress of germination to different percentiles,at sub-optimal and supra-optimal temperatures, respectively.Like germination rates, base temperature (Tb) declined from3.71 to –0.83 ?C as the percentile value increased from10% to 80%. Caution was urged in extrapolating beyond the experimentaldata set. Differences in the ceiling temperature (Tc) with percentilecould not be discerned. Cumulative germination progress curves at each temperature weremodelled by the Weibull, logistic, and cumulative normal distributionfunctions. Cardinal temperatures (Tb and Tc) calculated fromthese data reasonably approximated the actual data. The Weibullfunction demonstrated a good approximation at all percentilelevels, while the logistic and cumulative normal distributionfunctions, as a result of their inherent symmetry, deviatedat the extreme percentiles. It was concluded that the Weibullfunction not only accurately modelled cumulative germinationbut could also be used in the calculation of cardinal temperatures. Key words: Seed germination rate, cardinal temperatures, faba bean, Weibull function, probit and logic scales  相似文献   

17.
A linear relationship between constant temperatures in the sub-optimaltemperature range and germination rate is shown in both Quercusrobur L and Castanea sativa Mill germinated under nominal darkconditions. The mean base temperature was interpolated for Qrobur as 0 8 ? or 2-4 ?, depending on seed lot provenance, andfor C. sativa as 1 -4? The optimum temperature for germinationin Q. robur was about 20? compared with around 28 ? in C. sativaOver the sub-optimal temperature range the distribution of thermaltimes was log-normal for each population studied their spreadvarying both between Q robur seed lots and between species However,in C. sativa germinated close to the mean base temperature,the distribution in thermal times was reduced Thermal timesto germination were decreased in Q. robur and C sativa by approximately0 3 and 0-5 log-units, respectively, when the pericarp was removed,i.e in the seeds, but the sensitivity of the response remainedrelatively unaltered In both species the germination rate was the same when nominaldark or safe green light conditions were employed during thegermination test. However, at 21 ? Q robur exhibited the highirradiance reaction (HIR) at photon doses above 30mmol m–2d–1. HIR first affected the germination rate by an inhibitionof radicle extension The sensitivity of the response to thermaltime was reduced as photon dose increased. This photo-inhibitionwas exacerbated at supra-optimal temperatures. In contrast,C. sativa germination rate at 26 ? was little influenced bylight at a photon dose of 752 mmol m–2 d–1 Key words: Seed germination rate, temperature, thermal time, light, photon dose  相似文献   

18.
Germination of Tagetes minuta L. I. Temperature Effects   总被引:1,自引:0,他引:1  
Initial studies have indicated that Tagetes minuta achenes haveboth a temperature and a light requirement for germination.Temperatures tested were 10, 20, 25, 30 and 35 °C. Germinationwas optimal at 25 °C under white light conditions. Underthese conditions 100 per cent of achenes germinated within 7days of imbibition. There was no germination at 10 or 35 °Ceither in the light or in the dark. Achenes imbibed and incubatedat 35 °C for 4 days showed no visible signs of germinationbut on transfer to 25 °C, 100 per cent of these achenesgerminated within 24 h. Furthermore, achenes given this hightemperature (35 °C) treatment could be dried at 25 °C,re-imbibed at 25 °C and again 100 per cent of achenes germinatedwithin 24 h of re-imbibition. This rapid germination responsefollowing removal from the high temperature regime could alsobe induced by transfer to temperatures of 20 °C or 20 °C(16 h) alternating with 10 °C (8 h). Tagetes minuta L., weed seeds, germination, temperature, light  相似文献   

19.
The effects of alternating temperatures, using liquid nitrogen(–196°C) as the low temperature phase, on Trifolium arvense L.seed quality was investigated. Treatment reduced hardseedednesswith the seeds exhibiting a linear increase in the probit ofgermination with the logarithm of the number of alternatingtemperature cycles. Germination levels equal to ‘chipped’controls were achieved after four cycles or more, dependingon the initial depth of hardseededness, and the response wasunaffected by either the rate of cooling or the rate of warming. Imbibition time was reduced with increasing alternating temperaturecycles. Resealing experiments identified the main area of wateruptake as the lens, and SEM showed that deepening of alreadypresent cracks may be important in removing hardseededness.Pre-treatment of seeds with alternating temperatures predisposesthe seeds to subsequent damage by sulphuric acid treatment,but not by impaction. Stress effects were not restricted to the seed coat surfaceas between 10 and 20 % of seeds treated for 3–15 cyclesdeveloped into abnormal seedlings with detached cotyledons.Seed vigour is also reduced as treatment leads to contractionof the temperature response curve for germination. The resultshave implications for the cryopreservation of papilionoid legumeseeds as a means of genetic conservation. Trifolium arvense, Hare's foot, hardseededness, quantal response, cryopreservation, liquid nitrogen  相似文献   

20.
For a single seed population of each of four species of grainlegume positive linear relationships were shown between temperatureand rate of germination for different fractions (G) of eachpopulation, from a base temperature, Tb(G), at which germinationrate is zero, to an optimum temperature, To(G) at which germinationrate is maximal. At constant temperatures warmer than To(G)there were negative relations (probably linear) between temperatureand rate of germination to the maximum temperature for germination,Tm(G), Within each population Tb(G) did not differ, but it didvary between species, viz.0.0?C, 0.25?C, 4.and 8.5?C for chickpea(Cicer arietinum L.), lentil (Lens culinaris Medic.), soyabean(Glycine max [ Merr.) and cowpea (Vigna unguiculata [L.] Walp.),respectively. In contrast, To(G) varied both within each populationand also between the four species: 80% of seeds in each populationhad To(G) values within the range 31.8?C to 33.8 ?C, 24.0?Cto 24.4?C, 34.0?C to 34.5?C and 33.2?C to >40?C, respectively.Values of Tm(G) were much more vanable: the 80% population rangewas 48 .0?C to 60.8?C for chickpea, 31.8?C to 34.4?C for lentiland 46.8?C to 55.2?C for soyabean; reliable estimates couldnot be made for cowpea, but the results suggest higher and morevariable values of Tm(G) than in the other three species. Atsub-optimal temperatures the distribution of thermal time forthe different fractions of each population was normal, exceptfor lentil where it was log-normal. A single equation is proposedto describe the influence of sub-optimal temperatures on ratesof germination for whole seed populations. At supra-optimaltemperatures, variation in thermal time for the different fractionsof each population was only slight. The implications of thesefindings for the adaptation of grain legume crops to differentenvironments, and for the screening of germplasm, are discussed. Key words: Seed germination rate, temperature, grain legumes  相似文献   

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