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The Arabidopsis root transcriptome by serial analysis of gene expression. Gene identification using the genome sequence 总被引:5,自引:0,他引:5
Fizames C Muños S Cazettes C Nacry P Boucherez J Gaymard F Piquemal D Delorme V Commes T Doumas P Cooke R Marti J Sentenac H Gojon A 《Plant physiology》2004,134(1):67-80
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Migration and proliferation of vascular smooth muscle cells (SMCs) are key events in atherosclerosis. However, little is known about alterations in gene expression upon transition of the quiescent, contractile SMC to the proliferative SMC. We performed serial analysis of gene expression (SAGE) of cultured, human SMCs, either grown under resting circumstances or activated with an atherogenic stimulus. Analysis of tags, representing 47,209 and 47,259 mRNAs from a library of resting and activated SMCs, respectively, identified 105 tags induced and 52 tags repressed greater than fivefold. To evaluate the relevance in SMC biology of unmatched, regulated tags, we performed hierarchical clustering analysis, based on their expression profiles in public SAGE databases, and clustered these novel genes in distinct groups. The regulation in SMCs was confirmed by Northern blotting for representative genes of these groups. Plasminogen activator inhibitor-2 has not been associated with atherosclerosis before and was localized to atherosclerotic lesions. 相似文献
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Transcriptomes for serial analysis of gene expression 总被引:1,自引:0,他引:1
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