共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
3.
TGA1 and G-box binding factors: two distinct classes of Arabidopsis leucine zipper proteins compete for the G-box-like element TGACGTGG. 总被引:17,自引:6,他引:11
下载免费PDF全文
![点击此处可从《The Plant cell》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Regulatory elements containing the sequence ACGT are found in several plant promoters and are recognized by various basic/leucine zipper (bZIP) proteins. The Arabidopsis G-box binding factor 1 (GBF1), initially identified by its ability to bind to the palindromic G-box (CCACGTGG), also interacts with the TGACGT motif if this hexamer sequence is followed by either the dinucleotide GG--as found in the Hex motif of the wheat histone 3 promoter--or GT. Here we describe the isolation of an Arabidopsis bZIP protein, denoted TGA1, that also recognizes ACGT-containing sequences. However, TGA1 differs from members of the GBF family in the spectrum of base pair permutations flanking the ACGT sequence that are required for DNA binding. TGA1 primarily requires a TGACG motif and preferentially binds to those pentamers that are followed by a T residue. We show that although both TGA1 and GBF1 bind to the Hex motif (TGACGTGG), this binding can be distinguished on the basis of their specific DNA-protein contacts. Furthermore, TGA1 also differs from members of the GBF family in that it apparently does not form heterodimers with any member of this family. 相似文献
4.
5.
6.
7.
8.
9.
bZIP transcription factors in Arabidopsis 总被引:28,自引:0,他引:28
Jakoby M Weisshaar B Dröge-Laser W Vicente-Carbajosa J Tiedemann J Kroj T Parcy F;bZIP Research Group 《Trends in plant science》2002,7(3):106-111
10.
11.
Zhou JM Trifa Y Silva H Pontier D Lam E Shah J Klessig DF 《Molecular plant-microbe interactions : MPMI》2000,13(2):191-202
12.
13.
14.
15.
16.
In vitro binding of the tomato bZIP transcriptional activator VSF-1 to a regulatory element that controls xylem-specific gene expression 总被引:13,自引:1,他引:12
Sonia Torres-Schumann Christoph Ringli Daniel Heierli Nikolaus Amrhein Beat Keller 《The Plant journal : for cell and molecular biology》1996,9(3):283-296
The bean grp1.8 gene is specifically expressed in vascular tissue. Monomers and multimers of a 28 bp regulatory element of the grp1.8 promoter ( vs-1 ) specifically activated both the −82 CaMV 35S and the −76/ grp1.8 minimal promoters in vascular tissue of transgenic tobacco plants. vs-1 partially overlaps with a negative regulatory element in the grp1.8 promoter that is necessary for restriction of gene expression to vascular tissue. Nuclear extracts from tobacco and tomato cells contain a factor that binds to vs-1 in vitro . To study the molecular basis of xylem-specific expression mediated by the vs-1 promoter element, a gene was isolated from tomato encoding a protein that binds to vs-1 in vitro . This protein, designated VSF-1, contains a bZIP motif close to the C-terminus. Mutated vs-1 elements were no longer bound by VSF-1 and also failed to activate the minimal −82 CaMV 35S promoter in vivo . Transient expression of VSF-1 in protoplasts stimulated vs-1 dependent activation of the −76/ grp1.8 minimal promoter. Binding studies and use of a polyclonal antiserum against VSF-1 provided further evidence that vs-1 is a potential in vivo target site, as VSF-1 was a part of the observed complex formed between vs-1 and nuclear protein extract. vs-1 does not contain the 5'-ACGT-3' core sequence that is part of known plant bZIP protein binding sites or another palindromic sequence. Based on the unusual binding specificity and a characteristic amino acid sequence in the bZIP domain we propose that VSF-1 and the partially homologous PosF21, a bZIP protein from Arabidopsis , belong to a new family of plant bZIP proteins. 相似文献
17.
18.
19.
20.
Plant protein inhibitors of invertases 总被引:12,自引:0,他引:12