《农业植物病理学》课程网站的建设

在进行区级精品教材—《农业植物病理学》(华南本)的编著工作的同时,开展了《农业植物病理学》课程网站的开发设计,提出了网站的设计思路及网站建设的方法,并就其测试效果及性能进行了分析。     

植物分子育种方法研究进展

综述了各种植物外源遗传物质转移方法,就其原理和各自特点作了概述.并且展望了各种转基因方法的应用前景,提出多种转基因方法联合运用的优势.     

Isopropyl-Alcohol-Paraffin Methods for Plant Tissues

Two methods using isopropyl alcohol for dehydration prior to paraffin infiltration of plant tissues are reported: (1) a modified Rawlins-Takahashi (1947) schedule in which the preliminary dehydration is effected by concentrating glycerol and (2) isopropyl alcohol as the sole agent for dehydration. With the latter method the fixed tissues are dehydrated successfully in 60%, 85%, and 99% isopropyl alcohol. Paraffin infiltration is accomplished by placing the tissues in isopropyl alcohol over solid paraffin in a vial and heating to 56°-58° C. The tissues settle into the melted paraffin as infiltration progresses. Several changes of pure paraffin are then made, with the last change under reduced pressure. The embedded tissues are trimmed and soaked 2-4 hours at 40° C. in either water or a glycerol, acetic acid, 70% alcohol mixture (10:15:75) to reduce static and insure uniform ribboning during microtomy.     

Development of Electronic Barcodes for use in Plant Pathology and Functional Genomics

We have developed a novel ‘electronic barcode’ system that uses radio frequency identification (RFID) tags, cell phones, and portable computers to link phenotypic, environmental, and genomic data. We describe a secure, inexpensive system to record and retrieve data from plant samples. It utilizes RFID tags, computers, PDAs, and cell phones to link, record, and retrieve positional, and functional genomic data. Our results suggest that RFID tags can be used in functional genomic screens to record information that is involved in plant development or disease.     

植物细胞过氧化氢的测定方法

过氧化氢是重要的活性氧之一,激素等发育信号和胁迫刺激可以诱导细胞内H2O2的产生和积累,继而调控植物的气孔运动、生长发育、衰老和逆境应答等诸多生理过程。准确测定植物细胞内H2O2的含量及变化模式是系统研究H2O2信号转导及其生物学功能的一个关键技术。该文以拟南芥为实验材料,介绍了目前植物细胞H2O2的主要测定方法,包括激光共聚焦显微检测、紫外分光光度计检测和DAB组织染色,在此基础上比较分析了上述方法在灵敏度、检测范围、定量、成本以及耗时等方面的差异,为相关研究选择合适的H2O2检测技术提供参考。     

植物MicroRNA的特点与研究方法

MicroRNAs(miRNAs)是一类在植物、动物、单细胞藻类和病毒等中存在的具有调控基因表达作用的内源非编码小RNA(small RNAs).在植物中,miRNAs主要依靠与靶基因之间完全或近乎完全的互补配对切割靶基因或翻译抑制实现其调控功能.主要综述植物miRNA的特点,并介绍miRNA的获得方式、靶基因预测及验证方法.     

高通量植物蛋白质组学研究方法

模式植物拟南芥和水稻的基因组测序,使得大规模、高通量的研究方法在基因组和蛋白质组研究中日趋重要。本文综述双向电泳、质谱、蛋白质微阵列、抗体、酵母双杂交系统以及一些新型高通量方法研究进展及其在植物蛋白质组研究中的应用。