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Gian Luigi Gianfranceschi Donatella Barra Francesco Bossa Sandra Coderoni Mario Paparelli Franco Venanzi Fabio Cicconi Domenico Amici 《Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression》1982,699(2):138-148
Low-molecular-weight peptides are linked to the chromatin DNA of several tissues, from which they can be dissociated by alkaline extraction at pH 9.5. The level of the active peptide fraction ranges between 10 and 35 μg/mg DNA. The removal of peptides from DNA causes a relevant amplification of DNA template capacity for prokaryotic and eukaryotic RNA polymerases. Gel filtration on Sephadex G-25 or BioGel P4 shows that the chromatin peptide fraction from purified DNA migrates as a sharp peak with an elution volume corresponding to a molecular weight of about 1000. The chromatin peptides are further purified by Sephadex G-10 and high-performance liquid chromatography. Four active fractions are isolated, one of which shows very high inhibition activity on the RNA synthesis in vitro. The amino acid analysis and the inhibition mechanism of the purified peptides are reported. 相似文献
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G L Gianfranceschi D Barra F Bossa S Coderoni M Paparelli F Venanzi F Cicconi D Amici 《Biochimica et biophysica acta》1982,699(2):138-148
Low-molecular-weight peptides are linked to the chromatin DNA of several tissues, from which they can be dissociated by alkaline extraction at pH 9.5. The level of the active peptide fraction ranges between 10 and 35 micrograms/mg DNA. The removal of peptides from DNA causes a relevant amplification of DNA template capacity for prokaryotic and eukaryotic RNA polymerases. Gel filtration on Sephadex G-25 or BioGel P4 shows that the chromatin peptide fraction from purified DNA migrates as a sharp peak with an elution volume corresponding to a molecular weight of about 1000. The chromatin peptides are further purified by Sephadex G-10 and high-performance liquid chromatography. Four active fractions are isolated, one of which shows very high inhibition activity on the RNA synthesis in vitro. The amino acid analysis and the inhibition mechanism of the purified peptides are reported. 相似文献
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Li G Ruan X Auerbach RK Sandhu KS Zheng M Wang P Poh HM Goh Y Lim J Zhang J Sim HS Peh SQ Mulawadi FH Ong CT Orlov YL Hong S Zhang Z Landt S Raha D Euskirchen G Wei CL Ge W Wang H Davis C Fisher-Aylor KI Mortazavi A Gerstein M Gingeras T Wold B Sun Y Fullwood MJ Cheung E Liu E Sung WK Snyder M Ruan Y 《Cell》2012,148(1-2):84-98
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