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1.
Yang Wang Zhanfeng Xia Zhanwen Liu Chuanxing Wan Xiaoxia Luo Lili Zhang 《Antonie van Leeuwenhoek》2018,111(10):1807-1814
A novel actinobacterial strain, designated TRM SA0054T, was isolated from the roots of Sophora alopecuroides grown in Alar, Xinjiang, north-west China, and characterised by a polyphasic taxonomic approach. Phylogenetic analysis showed that strain TRM SA0054T has 16S rRNA gene sequence similarity of 98.22% with Streptomyces barkulensis RC 1831T. Whole cell hydrolysates of strain TRM SA0054T were found to contain ll-diaminopimelic acid as the diagnostic diamino acid and ribose, glucose and xylose as the major whole cell sugars. The major fatty acids were identified as iso-C16:0, iso-C16:1 G, anteiso-C17:0, anteiso-C15:0, C16:0, anteiso-C17:1 ω9c. The main menaquinones were determined to be MK-8 (H4), MK-9 (H6) and MK-9 (H8). The polar lipids were identified as diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified lipid. The G?+?C content of the genomic DNA was determined to be 73.04%. Strain TRM SA0054T has a relatively low DNA–DNA relatedness value with Streptomyces barkulensis RC 1831T as determined by calculating the average nucleotide identity value (ANI?=?84.1%). Based on the phenotypic, chemotaxonomic and phylogenetic data, it is concluded that strain TRM SA0054T should be designated as a novel species of the genus Streptomyces, for which the name Streptomyces carminius sp. nov. is proposed, with type strain TRM SA0054T (=?CCTCC AA 2016041T?=?KCTC39903T). 相似文献
2.
Liying Jin Junwei Zhao Shanwen Jiang Yang Zhao Xiaoyang Han Xiaowei Guo Xiangjing Wang Wensheng Xiang 《Antonie van Leeuwenhoek》2018,111(11):2079-2086
Two Gram-stain positive, aerobic actinomycete strains, designated NEAU-JGR1T and NEAU-JGC41, were isolated from soil collected from Fairy Lake Botanical Garden in Shenzhen, Guangdong Province, south of China. The 16S rRNA gene sequences analysis showed that the two strains exhibited 99.5% 16S rRNA gene sequence similarity with each other and were closely related to Promicromonospora thailandica JCM 17130T (99.4, 99.3%) and Promicromonospora citrea DSM 43110T (99.2, 99.2%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two strains clustered together and formed a cluster with P. thailandica JCM 17130T and P. citrea DSM 43110T. Both strains were observed to contain MK-9(H4) and MK-9(H2) as predominant menaquinones. Their whole cell sugar profiles were found to main contained rhamnose, ribose, glucose and galactose. The phospholipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycophosphatidylinositol, phosphatidylinositol mannoside, an unidentified glycolipid and an unidentified phospholipid. The predominant cellular fatty acids for the two strains were identified as anteiso-C15:0, iso-C15:0 and anteiso-C17:0. The DNA–DNA hybridization value between strains NEAU-JGR1T and NEAU-JGC41 was 85.1?±?0.3%, and the values between the two strains and their close phylogenetic relatives were well below 70%, supporting the conclusion that they represent a distinct genomic species. An array of phenotypic characteristics also differentiated the isolates from closely related species. On the basis of the genetic and phenotypic properties, strains NEAU-JGR1T and NEAU-JGC41 can be classified as representatives of a novel species of the genus Promicromonospora, for which the name Promicromonospora viridis sp. nov., is proposed. The type strain is NEAU-JGR1T (=?DSM 105536T?=?CGMCC 4.7473T). 相似文献
3.
Adel Aouiche Noureddine Bouras Salim Mokrane Abdelghani Zitouni Peter Schumann Cathrin Spröer Nasserdine Sabaou Hans-Peter Klenk 《Antonie van Leeuwenhoek》2015,107(1):291-296
A novel actinomycete strain, designated PAL84, was isolated from a Saharan soil sample collected from Béni-Isguen, Ghardaïa (South of Algeria). This strain was studied for its taxonomic position using a polyphasic approach and was identified as a member of the genus Actinokineospora. Phylogenetic analysis showed that strain PAL84 had 16S rRNA gene sequence similarities with members of the genus Actinokineospora ranging from 96.2 % (Actinokineospora inagensis DSM 44258T) to 97.8 % (Actinokineospora baliensis NBRC 104211T). The strain was observed to produce pinkish-purple aerial mycelium and purplish red substrate mycelium, which fragmented readily into chains of non-motile elements. The optimum growth temperature and pH were found to be 25–30 °C and 5.0–7.0, respectively. The cell-wall hydrolysate of strain PAL84 was found to contain meso-diaminopimelic acid and the diagnostic whole-cell sugars were identified as arabinose and galactose. The predominant menaquinone was identified as MK-9 (H4). The major fatty acids were found to be iso-C16:0, iso-C15:0, iso-C16:1 H and iso-C16:0 2OH. The diagnostic phospholipid detected was phosphatidylethanolamine. The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinokineospora, for which the name Actinokineospora mzabensis sp. nov. is proposed, with the type strain PAL84T (=DSM 45961T = CECT 8578T). 相似文献
4.
Junwei Zhao Dongmei Li Hao Jiang Liyuan Han Shanwen Jiang Xiaowei Guo Xiangjing Wang Wensheng Xiang 《Antonie van Leeuwenhoek》2018,111(12):2249-2256
A novel actinomycete, designated strain NEAU-LA29T, was isolated from soil collected from Xianglu Mountain and subjected to a polyphasic taxonomic study. Based on a polyphasic taxonomic approach comprising chemotaxonomic, phylogenetic, morphological and physiological characterisation, the isolate has been affiliated to the genus Streptomyces. 16S rRNA gene sequence analysis showed that the isolate is closely related to Streptomyces vastus JCM4524T (98.8% identity) and Streptomyces cinereus DSM43033T (97.9%). However, multilocus sequence analysis based on five other house-keeping genes (atpD, gyrB, rpoB, recA and trpB) and low DNA–DNA relatedness values enabled the strain to be differentiated from these closely related species of the genus Streptomyces. Thus, strain NEAU-LA29T is concluded to represent a novel species of the genus Streptomyces, for which the name Streptomyces xiangluensis sp. nov. is proposed. The type strain is NEAU-LA29T (=?CGMCC 4.7466T?=?DSM 105786T). 相似文献
5.
Yue Shen Tianyu Sun Shanwen Jiang Shan Mu Dongmei Li Xiaowei Guo Jianli Zhang Junwei Zhao Wensheng Xiang 《Antonie van Leeuwenhoek》2018,111(12):2403-2412
A novel Gram-stain positive, spore-forming, aerobic actinomycete, designated strain NEAU-QTH3-11T, was isolated from muddy soil collected from a stream in Qitaihe, Heilongjiang Province, northeast China and characterised using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-QTH3-11T belongs to the genus Streptomyces and is closely related to Streptomyces rhizosphaerihabitans NBRC 109807T (99.38%) and Streptomyces mirabilis JCM 4791T (99.03%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain formed a cluster with S. rhizosphaerihabitans NBRC 109807T and Streptomyces siamensis NBRC 108799T (98.62%). The menaquinones were identified as MK-9(H8), MK-9(H6) and MK-9(H4). The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as anteiso-C15:0, iso-C16:0, C16:0 and C15:0. However, multilocus sequence analysis based on five house-keeping genes (atpD, gyrB, rpoB, recA and trpB), low DNA-DNA hybridization results and some phenotypic, physiological and biochemical properties could differentiate the strain from its close relatives in the genus Streptomyces. Therefore, strain NEAU-QTH3-11T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces lutosisoli sp. nov. is proposed, with NEAU-QTH3-11T (=DSM 42165T=CGMCC 4.7198T) as the type strain. 相似文献
6.
An actinomycete strain, designated YIM 60475T, was isolated from the roots of Maytenus austroyunnanensis and was characterized by using a polyphasic approach. The strain was determined to belong to the genus Streptomyces, based on its phenotypic and phylogenetic characteristics. The strain produced spiral spore chains on aerial mycelium. The
cell wall contained ll-diaminopimelic acid. Whole-cell hydrolysates contained galactose, glucose, and xylose. The phospholipid was type II. The
DNA G+C content of the type strain was 73.3 mol%. DNA–DNA hybridization and comparison of physiological and chemical characteristics
suggested that strain YIM 60475T is a new Streptomyces species, for which the name Streptomyces mayteni sp. nov. is proposed. The type strain is YIM 60475T (=CCTCC AA 207005T = KCTC 19383T).
Hua-Hong Chen and Sheng Qin contributed equally to this work. 相似文献
7.
An actinomycete strain, which was designated 172115T, was isolated from mangrove soil in Shenzhen, China. Strain 172115T fell within the genus Streptomyces in the 16S rRNA gene tree and could be grouped into this genus based on its chemotaxonomic and morphological data. The strain
shared the highest 16S rRNA gene sequence similarities with Streptomyces lanatus NBRC 12787T (AB184845) (98.29%) and Streptomyces lucensis NBRC 13056T (AB184280) (98.26%). The DNA–DNA hybridization values between strain 172115T and the two most closely related type strains were low enough to justify the assignment of the strain to a novel species.
On the basis of these phenotypic, phylogenetic and chemotaxonomic characteristics, 172115T represents a novel species of the genus Streptomyces, for which, the name Streptomyces shenzhenensis sp. nov. is proposed for strain 172115T (=CCTCC AA 2011001T=DSM 42034T). 相似文献
8.
9.
Guan TW Wu N Xia ZF Ruan JS Zhang XP Huang Y Zhang LL 《Extremophiles : life under extreme conditions》2011,15(3):373-378
A novel actinomycete strain, designated TRM 40133T, was isolated from a hypersaline habitat of Tarim basin in Xinjiang Province, north-west China. Its taxonomic status was
determined using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of the strain
showed that it formed a well-seperated sub-branch within the radiation of the genus Saccharopolyspora. The highest levels of 16S rRNA gene sequence similarity was found between the strain TRM 40133T and Saccharopolyspora qijiaojingensis YIM 91168T (96.5%). The chemotaxonomic characteristics of the isolate are typical for the genus Saccharopolyspora. It contained meso-DAP as the diagnostic diamino acid. Whole cell hydrolysate contained arabinose, xylose, ribose and glucose. The diagnostic
phospholipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and two unknown phospholipids. The main
menaquinone was MK-9(H6) and MK-9(H4). No mycolic acid was detected. The predominant cellular fatty acids were iso-C16:0 and anteiso-C17:0. The G+C content of the genomic DNA was 68.2 mol%. In addition, the strain TRM 40133T had a phenotypic profile that readily distinguished it from the recognized representatives of the genus Saccharopolyspora. The strain TRM 40133T therefore represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora lacisalsi sp. nov. is proposed. The type strain is TRM 40133T (=KCTC 19987T =CCTCC AA 2010012T). 相似文献
10.
Csotonyi JT Stackebrandt E Swiderski J Schumann P Yurkov V 《Archives of microbiology》2011,193(8):573-582
A strain EG19T of aerobic bacteria able to form pleomorphic cells was isolated from a brine spring runoff stream in the west central region
of the province of Manitoba, Canada. The pale pinkish purple strain contained bacteriochlorophyll a incorporated into light-harvesting I and reaction center complexes. Its inability to grow under anaerobic illuminated conditions
prompted designation as a member of the functional group known as aerobic anoxygenic phototrophic bacteria. Phylogenetic analysis
of the 16S rRNA gene sequence revealed that it belonged to the Gammaproteobacteria, forming a distinct branch of phototrophs distantly related to most described aerobic anoxygenic phototrophs, quite marginally
related (95.6%) both to the only other described gammaproteobacterial aerobic phototroph, Congregibacter litoralis, and also to nonphototrophs in the genus Haliea (95.1–96.1%). Physiological tests demonstrated tolerance profiles to salinity (0–18% NaCl), pH (7–12), and temperature (7–40°C)
consistent with survival in a shallow hypersaline stream on the exposed, vegetation-depleted salt playa of its native East
German Creek. Phylogenetic data and phenotypic properties such as pigment composition, morphology, and physiology support
the proposal of the novel genus and species Chromocurvus halotolerans gen. nov., sp. nov., with EG19T (=DSM 23344T, =VKM B-2659T) as the type strain. 相似文献
11.
Taxonomic studies were performed on three strains isolated from Cheonho reservoir in Cheonan, Korea. The isolates were Gram-negative,
aerobic, rod-shaped, non-motile, catalase-positive, and oxidase-positive. Colonies on solid media were cream-yellow, smooth,
shiny, and circular. Phylogenetic analysis of the 16S rRNA gene sequences revealed that these strains belong to the genus
Flavobacterium. The strains shared 98.6–99.4% sequence similarity with each other and showed less than 97% similarity with members of the
genus Flavobacterium with validly published names. The DNA-DNA hybridization results confirmed the separate genomic status of strains ARSA-42T, ARSA-103T, and ARSA-108T. The isolates contained menaqui-none-6 as the predominant menaquinone and iso-C15:0, iso-C15:0 3-OH, iso-Ci15:1 G, and iso-C16:0 3-OH as the major fatty acids. The genomic DNA G+C content of the isolates were 31.4–33.2 mol%. According to the phenotypic
and genotypic data, these organisms are classified as representative of three novel species in the genus Flavobacterium, and the name Flavobacterium koreense sp. nov. (strain ARSA-42T =KCTC 23182T =JCM 17066T =KACC 14969T), Flavobacterium chungnamense sp. nov. (strain ARSA-103T =KCTC 23183T =JCM 17068T =KACC 14971T), and Flavobacterium cheonanense sp. nov. (strain ARSA-108T =KCTC 23184T =JCM 17069T =KACC 14972) are proposed. 相似文献
12.
Syed DG Agasar D Kim CJ Li WJ Lee JC Park DJ Xu LH Tian XP Jiang CL 《Antonie van Leeuwenhoek》2007,92(4):391-397
A Gram-positive, nonmotile, moderately halophilic, alkali and thermotolerant strain designated DAS 165(T), was isolated from a dry land soil sample from the Gulbarga region, Karnataka province, India. The isolate produced yellow substrate mycelia and gray aerial mycelia on most tested media. Strain DAS 165(T) showed growth in the presence of 5 to 7% NaCl and at 45 degrees C. The DNA G + C content was 69.7%. 16S rRNA gene sequence analysis together with these characteristics consistently assigned strain DAS 165(T) to the genus Streptomyces. The 16S rRNA gene sequence analysis revealed that strain DAS 165(T) was most closely related to S. tendae ATCC 19812(T) (D 63873) with a sequence similarity of 99.6% (three nucleotide differences out of 1,517). Strain DAS 165(T) formed a distinct clade based on analysis of the almost complete sequence and 120-nucleotide variable gamma region of the 16S rRNA gene. Despite the high sequence similarity, strain DAS 165(T) was phenotypically different from S. tendae ATCC 19812(T). DNA-DNA hybridization between these strains was 47% showing that strain DAS 165(T) is a distinct genomic species. Phenetic and genetic results support the classification of strain DAS 165(T) as a new species, for which the name S. tritolerans is proposed, with strain DAS 165(T) as the type strain (=DSM 41899(T )= CCTCCAA 206013(T)). 相似文献
13.
During the course of our research on new actinobacterial sources, a novel actinomycete strain YIM 63101T was isolated from the surface-sterilized roots of Artemisia annua L. collected from Xishuangbanna, Yunnan province, south-west China and characterized by using a polyphasic approach. The
strain formed well-differentiated aerial and substrate mycelia. Phylogenetic analysis based on 16S rRNA gene sequences showed
that strain YIM 63101T belongs to the genus Pseudonocardia, with highest similarity to “Pseudonocardia artemisiae YIM 63587T” (99.4%). Sequence similarities between strain YIM 63101T and the other Pseudonocardia species ranged from 97.0 (Pseudonocardia saturnea IMSNU 20052T) to 94.0% (Pseudonocardia compacta IMSNU 20111T). The chemotaxonomic characteristics, such as cell wall diaminopimelic acid, whole-cell sugars, fatty acid components and
the major menaquinones suggested that the organism belonged to the genus Pseudonocardia. The G + C content of the genomic DNA was 69.4 mol%. Based on comparative analysis of physiological, biochemical and chemotaxonomic
data, including low DNA–DNA hybridization results, it is proposed that strain YIM 63101T represents a novel species of the genus Pseudonocardia, named Pseudonocardia bannaensis sp. nov. The type strain is YIM 63101T (= CCTCC AA 208077 T = DSM 45300T). 相似文献
14.
A strain YIM 33098T (= CCTCC AA001027T = DSM 41831T) was isolated from a forest soil sample collected from Nanning in Guangxi Province, China, in the course of screening for producers of new drug lead compounds. This strain was identified by using a polyphasic approach. The results showed that it should be assigned to the genus Streptomyces. An almost complete 16S rRNA gene sequence of the strain was determined and compared with those of representative Streptomyces species. Strain YIM 33098T was clustered in the same subclade with Streptomyces tendae ATCC19812T and Streptomyces eurythermus ATCC14975T. Similarities of strain YIM 33098T with the two strains were 97.35% and 97.42%, respectively. Based on the phenotypic and genotypic evidence, it is therefore proposed that strain YIM 33098T should be classified in the genus Streptomyces as a new species under the name of Streptomyces nanningensis sp. nov. 相似文献
15.
Miao Q Qin S Bian GK Yuan B Xing K Zhang YJ Li Q Tang SK Li WJ Jiang JH 《Antonie van Leeuwenhoek》2011,100(3):333-339
A novel actinomycete, designated KLBMP 1221T, was isolated from the surface-sterilized seeds of an oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China and was characterized taxonomically by using a polyphasic approach.
Phylogenetic analyses based on 16S rRNA gene sequence showed that this strain formed a distinct phyletic line within the radiation
of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1221T was most closely related to Amycolatopsis eurytherma NT202T (98.9%), Amycolatopsis tucumanensis ABOT (98.8%), Amycolatopsis thermoflava N1165T (98.6%) and Amycolatopsis methanolica IMSNU 20055T (98.5%). Strain KLBMP 1221T had morphological and chemotaxonomic properties that were consistent with its classification in the genus Amycolatopsis. However, DNA–DNA relatedness data and phenotypic differences clearly distinguished the isolate from its closest relatives.
Based on the combined genotypic and phenotypic evidence, it is proposed that strain KLBMP 1221T be classified as representative of a novel species for which the name Amycolatopsis
endophytica sp. nov. is proposed. The type strain is KLBMP 1221T (= KCTC 19776T = CCTCC AA 2010003T). 相似文献
16.
An aerobic, non-motile, catalase-positive, Gram-stain positive actinomycete designated YIM 63233T was isolated from the surface-sterilized leaves of Artemisia annua L. and characterized using a polyphasic taxonomic approach. Optimal growth occurred at 20–28°C, pH 6.0–7.0 and in the presence
of 0–3% (w/v) NaCl. 16S rRNA gene sequence-based phylogenetic analysis showed that strain YIM 63233T clustered with species of the genus Pseudonocardia, displaying ≥1.2% sequence divergence with recognized species of this genus (from 98.8 to 94.0%). Relatively low levels of
DNA–DNA relatedness were found between strain YIM 63233T and Pseudonocardia petroleophila IMSNU 22072T, which supported the classification of strain YIM 63233T within a novel species of the genus Pseudonocardia. The G + C content of genomic DNA was 72.0 mol%. Strain YIM 63233T possessed chemotaxonomic markers that were consistent with classification in the genus Pseudonocardia, i.e. the predominant fatty acids were iso-C16:0 (32.27%), C16:0 10-methyl (8.73%) and C17:1ω8c (8.30%), whilst the predominant
menaquinone was MK-8(H4). The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid. The major cell wall sugars were glucose, arabinose, galactose, mannose and rhamnose. The results of
physiological and biochemical tests and DNA–DNA hybridization allowed the phenotypic and genotypic differentiation of strain
YIM 63233T from its closest phylogenetic neighbours. Therefore, the new isolate YIM 63233T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia serianimatus sp. nov. is proposed. The type strain is YIM 63233T (=DSM 45302T = CCTCC AA 208079T). 相似文献
17.
A halotolerant actinomycete strain, designated XHU 5031T, was isolated from a salt lake in Xinjiang Province, northwest China. Its taxonomic status was determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was most closely related to Myceligenerans xiligouense DSM 15700T (98.4 %), Myceligenerans halotolerans XJEEM 11063T (98.0 %) and Myceligenerans crystallogenes DSM 17134T (97.5 %). However, it had relatively low values for DNA–DNA relatedness with the above strains (46.2, 39.4 and 36.5 %, respectively). The peptidoglycan type was A4α. This organism contained glucose, mannose and galactose as the major whole cell sugars. The predominant menaquinone was MK-9(H4). The major fatty acids were iso-C15:0, anteiso-C15:0 and iso-C16:0. The polar lipids detected were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), one unknown phospholipid (PL) and two unknown glycolipids (GL). The G+C content of genomic DNA was 71.2 mol %. Phenotypic data clearly distinguished the isolate from its closest relatives. The combined genotypic and phenotypic data indicated that the isolate XHU 5031T represented a novel species of the genus Myceligenerans. The proposed name for this organism is Myceligenerans salitolerans sp. nov., with type strain XHU 5031T (=KCTC 29128T = CCTCC AB 2012908T). 相似文献
18.
Yang Ji Wei-Xun Chunyu En-Yuan Li Zhang-Gui Ding Min Yin Yu-Rong Zhao Yun Wang Shu-Kun Tang 《Antonie van Leeuwenhoek》2018,111(1):27-34
A novel aerobic, Gram-positive, non-motile halotolerant actinobacterium, designated strain YIM 96448T, was isolated from a saline soil sample collected from the south bank of Aiding Lake in Xinjiang Province, northwest China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 96448T is closely related to Phytoactinopolyspora endophytica EGI 60009T (98.0%) and Phytoactinopolyspora alkaliphila EGI 80629T (97.0%). Optimum growth of the strain was observed at 28-37 °C, pH 7–9 and in the presence of 5–8% NaCl (w/v). The respiratory quinone of strain YIM 96448T was determined to be MK-9(H4). The major fatty acids (>10%) were identified as anteiso-C15:0, iso-C16:0, anteiso-C17:0 and summed feature 4 (iso-C17:1 I and/or anteiso-C17:1B). The polar lipid profile was found to consist of diphosphatidylglycerol, an unidentified aminophospholipid, phosphatidylglycerol, two phosphatidylinositol mannosides, three unidentified phosphoglycolipids and four unidentified phospholipids. The DNA G+C content was determined to be 68.7 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain YIM 96448T should be classified as a novel species in the genus Phytoactinopolyspora, for which the name Phytoactinopolyspora halotolerans sp. nov. is proposed. The type strain is YIM 96448T (=CCTCC AB 2017055T = KCTC 39924T). 相似文献
19.
A Gram-positive, rod-shaped, motile and spore-forming bacterium, designated ZLD-8T, was isolated from a desert soil sample collected from Xinjiang Province in north-west China, and subjected to a polyphasic
taxonomic analysis. This isolate grew optimally at 30°C and pH 7.0. It grew with 0–4% NaCl (optimum, 0–1%). Comparative 16S
rRNA gene sequence analysis showed that strain ZLD-8T was closely related to members of the genus Bacillus, exhibiting the highest 16S rRNA gene sequence similarity to Bacillus kribbensis DSM 17871T (98.0%). The levels of 16S rRNA gene sequence similarity with respect to other Bacillus species with validly published names were less than 96.3%. The DNA G + C content of strain ZLD-8T was 40.1 mol%. The strain contained MK-7 as the predominant menaquinone. The diagnostic diamino acid in the cell-wall peptidoglycan
was meso-diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
The major fatty acids (>5% of total fatty acids) were anteiso-C15:0 (39.56%), iso-C14:0 (25.69%), C16:1 ω7c alcohol (10.13%) and iso-C15:0 (5.27%). These chemotaxonomic results supported the affiliation of strain ZLD-8T to the genus Bacillus. However, low DNA–DNA relatedness values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation
of strain ZLD-8T from recognized Bacillus species. On the basis of the polyphasic evidence presented, strain ZLD-8T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus
deserti sp. nov. is proposed. The type strain is ZLD-8T (=CCTCC AB 207173T = KCTC 13246T). 相似文献