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1.
A comparison of whole-fish polychlorinated biphenyl (PCB) and total mercury (Hg) concentrations in mature males with those in mature females may provide insights into sex differences in behavior, metabolism, and other physiological processes. In eight species of fish, we observed that males exceeded females in whole-fish PCB concentration by 17 to 43 %. Based on results from hypothesis testing, we concluded that these sex differences were most likely primarily driven by a higher rate of energy expenditure, stemming from higher resting metabolic rate (or standard metabolic rate (SMR)) and higher swimming activity, in males compared with females. A higher rate of energy expenditure led to a higher rate of food consumption, which, in turn, resulted in a higher rate of PCB accumulation. For two fish species, the growth dilution effect also made a substantial contribution to the sex difference in PCB concentrations, although the higher energy expenditure rate for males was still the primary driver. Hg concentration data were available for five of the eight species. For four of these five species, the ratio of PCB concentration in males to PCB concentration in females was substantially greater than the ratio of Hg concentration in males to Hg concentration in females. In sea lamprey (Petromyzon marinus), a very primitive fish, the two ratios were nearly identical. The most plausible explanation for this pattern was that certain androgens, such as testosterone and 11-ketotestosterone, enhanced Hg-elimination rate in males. In contrast, long-term elimination of PCBs is negligible for both sexes. According to this explanation, males not only ingest Hg at a higher rate than females but also eliminate Hg at a higher rate than females, in fish species other than sea lamprey. Male sea lamprey do not possess either of the above-specified androgens. These apparent sex differences in SMRs, activities, and Hg-elimination rates in teleost fishes may also apply, to some degree, to higher vertebrates including humans. Our synthesis findings will be useful in (1) developing sex-specific bioenergetics models for fish, (2) developing sex-specific risk assessment models for exposure of humans and wildlife to contaminants, and (3) refining Hg mass balance models for fish and higher vertebrates. 相似文献
2.
P. G. H. EVANS 《Mammal Review》1982,12(4):187-206
(1) Seabird-cetacean associations involve many genera.
(2) Some seabirds/cetaceans associate with more species than others, e.g. minke and pilot whales, Common dolphins and porpoises amongst cetaceans, and gannet & kittiwake amongst seabirds. Killer whale, sperm whale, shag and cormorant have not been recorded in associations, in the N.E. Atlantic.
(3) All observed associations would be predicted on the basis of the diets of the associated species.
(4) Most associations are probably opportunistic or incidental, as a result of concentration of shared prey.
(5) Some species, however, may associate more regularly, e.g. minke & pilot whales, Common dolphin and porpoise amongst cetaceans and gannet amongst seabirds. It is not possible to say which derives benefit from the association, but on theoretical grounds it is most likely to be the seabird.
(6) Minke and pilot whales may drive towards the surface food normally out of reach of seabirds, particularly Procellariformes. Common dolphins feed by herding fast-moving fish shoals into tight groups which will be a more concentrated food source for diving sea-birds, such as gannets, and make food more accessible to other species. Porpoises may achieve the same result with inshore shoals of sprats and sand eels, particularly for auks and kittiwakes, and terns. 相似文献
(2) Some seabirds/cetaceans associate with more species than others, e.g. minke and pilot whales, Common dolphins and porpoises amongst cetaceans, and gannet & kittiwake amongst seabirds. Killer whale, sperm whale, shag and cormorant have not been recorded in associations, in the N.E. Atlantic.
(3) All observed associations would be predicted on the basis of the diets of the associated species.
(4) Most associations are probably opportunistic or incidental, as a result of concentration of shared prey.
(5) Some species, however, may associate more regularly, e.g. minke & pilot whales, Common dolphin and porpoise amongst cetaceans and gannet amongst seabirds. It is not possible to say which derives benefit from the association, but on theoretical grounds it is most likely to be the seabird.
(6) Minke and pilot whales may drive towards the surface food normally out of reach of seabirds, particularly Procellariformes. Common dolphins feed by herding fast-moving fish shoals into tight groups which will be a more concentrated food source for diving sea-birds, such as gannets, and make food more accessible to other species. Porpoises may achieve the same result with inshore shoals of sprats and sand eels, particularly for auks and kittiwakes, and terns. 相似文献
3.
D. Lamb 《Plant and Soil》1977,47(2):495-508
Summary Relationships beween nutrient concentrations and the growth of the fast growing tropical eucalypt E. deglupta were examined at two sites in Papua New Guinea. At the Gogol Valley site a predominantly linear relationship was found between growth and foliar N over the range 0.68–2.04 per cent N. At the Kerevat site the relationship between growth and foliar nutrients was less strongly developed (foliar N varied from 1.77 to 3.36 per cent). The combined data could be described by a second degree polynomial. Height=11.05N – 2.03N2 – 3.52P – 4.46.This accounted for 72 per cent of the variation in height. Based on this relationship a tentative critical foliar N concentration (corresponding with 90 per cent of maximum height) of 2.1 per cent is proposed. re]19760128 相似文献
4.
LEONARD A. SMOCK 《Freshwater Biology》1983,13(4):313-321
SUMMARY.
- 1 The influence of organism size on whole-body metal concentrations of eight metals was examined in aquatic insects in field and laboratory studies. Information on the partitioning of metals between adsorbed, absorbed and gut content material was also obtained.
- 2 For Co, Cr, Fe, Sb and Sc, an exponential decrease in concentration with increasing organism size was observed, indicating surface adsorption as an important mode of metal accumulation.
- 3 No, or only a slight, concentration-size relationship was found for K, Mn and Na. This is expected for metals with high absorption efficiencies and a low capacity to be adsorbed.
- 4 Clearing of the gut of the mayfly Stenonema modestum (Ephemeroptera: Heptageniidae) did not significantly after the concentration size relationship for any of the metals studied.
- 5 Studies of Cr uptake with Stenacron interpunctatum (Ephemeroptera: Heptageniidae) indicate that the concentration-size relationship is not affected by variability in the concentration of available Cr.
- 6 About 52% of the whole-body burden of Cr in S. interpunctatum was associated with gut material while at least 33% was adsorbed to the organism's exoskeleton and at most 15% was internally absorbed.
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C. Leifert†‡ W.M. Waites† Helen Camotta‡ J.R. Nicholas†‡ 《Journal of applied microbiology》1989,67(4):363-370
Lactobacillus plantarum was isolated from in vitro plant cultures of Hemerocallis Stella d'ora, Catherine Woodbury and Stafford. Infected cultures deteriorated rapidly during three subcultures (15 weeks) when grown in vitro , showing chlorotic white shoots and grey calli. Weaned plants developed normally when transferred to the soil. The drop in multiplication rate of plant cultures coincided with a decrease in pH of the growth media. Uninfected plants of Hemerocallis Stella d'ora showed the same symptoms after inoculation with L. plantarum. Lactobacillus plantarum was reisolated from inoculated plant cultures that showed symptoms, thus fulfilling Koch's postulates. In plants inoculated with L. plantarum both the amount of dl-lactic acid formed and the number of plants showing symptoms increased with increasing numbers of bacteria in the inoculum, wheras plant multiplication rate and pH decreased. These effects could be reproduced by adding dl-lactic acid to the multiplication medium of plants free from L. plantarum , suggesting that the bacterial production of lactic acid was responsible for the changes in infected plants. 相似文献
8.
Mechanical factors modulate the morphogenesis and regeneration of mesenchymally derived tissues via processes mediated by the extracellular matrix (ECM). In distraction osteogenesis, large volumes of new bone are created through discrete applications of tensile displacement across an osteotomy gap. Although many studies have characterized the matrix, cellular and molecular biology of distraction osteogenesis, little is known about relationships between these biological phenomena and the local physical cues generated by distraction. Accordingly, the goal of this study was to characterize the local physical environment created within the osteotomy gap during long bone distraction osteogenesis. Using a computational approach, we quantified spatial and temporal profiles of three previously identified mechanical stimuli for tissue differentiation-pressure, tensile strain and fluid flow-as well as another candidate stimulus-tissue dilatation (volumetric strain). Whereas pressure and fluid velocity throughout the regenerate decayed to less than 31% of initial values within 20 min following distraction, tissue dilatation increased with time, reaching steady state values as high as 43% strain. This dilatation created large reductions and large gradients in cell and ECM densities. When combined with previous findings regarding the effects of strain and of cell and ECM densities on cell migration, proliferation and differentiation, these results indicate two mechanisms by which tissue dilatation may be a key stimulus for bone regeneration: (1) stretching of cells and (2) altering cell and ECM densities. These results are used to suggest experiments that can provide a more mechanistic understanding of the role of tissue dilatation in bone regeneration. 相似文献
9.
It has been previously shown that triglycerides can be specifically hydrolyzed by lipase from Rhizopus arrhizus in the presence of hog liver esterase and sodium dodecyl sulfate. The glycerol produced can then be measured by sequential reactions with glycerokinase, pyruvate kinase, and lactate dehydrogenase: glycerol and ATP are converted to glycerol-3-phosphate and ADP by glycerokinase; the ADP reacts with phosphoenolpyruvate and pyruvate kinase to yield pyruvate; the pyruvate is converted to lactate with lactate dehydrogenase, and the cofactor NAD+ is simultaneously reduced to NADH. This report describes procedures by which either the disappearance of NADH or the appearance of NAD+ was determined fluorometrically, with 10- to 100-fold greater sensitivity than by spectrophotometry. In addition, enzymatic cycling of NAD+ was used to increase the sensitivity of the assay over 1000-fold, and thereby provided accurate measurement of less than 1 ng of triglyceride. Results obtained from the three fluorometric methods were highly correlated with an automated periodate oxidation method using serum samples and lipid extracts of muscle tissue. 相似文献
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The metabolism and toxicity of formaldehyde (CH2O) in isolated rat hepatocytes was found to be dependent upon the intracellular concentration of glutathione (GSH). Using hepatocytes depleted of GSH by treatment with diethyl maleate (DEM), the rate of CH2O (5.0 mM) disappearance was significantly decreased. Formaldehyde decreased the concentration of GSH in hepatocytes, probably by the extrusion of the CH2O-GSH adduct, S-hydroxymethylglutathione. Formaldehyde toxicity was potentiated in cells pretreated with 1.0 mM DEM as measured by the loss of membrane integrity (NADH stimulation of lactate dehydrogenase (LDH) activity) and an increase in lipid peroxidation (formation of thiobarbituric acid-reactive compounds). This potentiation of toxicity was both CH2O concentration-dependent and time-dependent. There was an excellent correlation between the increase in lipid peroxidation and the decrease in cell viability. L-Methionine (1.0 mM) both protected the cells from toxicity caused by the combination of 8.0 mM CH2O and 1.0 mM DEM and increased the cellular GSH concentration. The antioxidants, ascorbate, butylated hydroxytoluene (BHT) and alpha-tocopherol (10, 25 and 125 microM), all exhibited dose-dependent protection against toxicity produced by 8.0 mM CH2O and 1.0 mM DEM. At toxic concentrations of CH2O (10.0-13.0 mM), administered by itself, lipid peroxidation did not increase concomitantly with the decrease in cell viability and the addition of antioxidants (125 microM) did not influence CH2O toxicity. These results suggest that CH2O toxicity in GSH-depleted hepatocytes may be mediated by free radicals as a result of the effect of CH2O on a critical cellular pool of GSH. However, cells with normal concentrations of GSH are damaged by CH2O by a different mechanism. 相似文献
12.
Díaz C López F Henríquez P Rodríguez E Serra-MaJem L 《Biological trace element research》2001,80(1):43-51
Serum manganese (Mn) concentrations of 368 individuals 6–75 yr of age (179 males and 189 females) living in the Canary Islands
were determined by graphite furnace atomic absorption spectrometry. The mean manganese concentration was 1.06 ± 0.62 μg/L,
ranging between 0.19 and 3.33 μg/L. Most of the analyzed samples (63.3% of the samples) fall within the reference interval
(0.54–1.76 μg/L) for apparently healthy people. Individuals from Fuerteventura presented with mean Mn concentrations significantly
higher than individuals from the rest of the islands. This could be attributed to differences in the Mn content of soil and/or
differences in dietary habits of the population. Serum Mn concentration did not vary with gender, and individuals younger
than 18 yr old had the highest mean Mn concentration, compared to the rest of the age groups considered. No relation to socioeconomic
status, educational level, and tobacco or alcohol consumption was found. However, the serum Mn concentration tended to decrease
when increasing the consumption of wine or beer. Sportsmen presented significantly higher serum Mn concentrations than the
rest. 相似文献
13.
Isenegger DA Taylor PW Mullins K McGregor GR Barlass M Hutchinson JF 《Plant cell reports》2003,21(8):814-820
An aberrant random amplified polymorphic DNA (RAPD) marker in genomic DNA of tissue culture plantlets was frequently observed during a comparison of DNA fingerprints derived from potato germplasm grown in tissue culture and the field. The RAPD marker was cloned, sequenced and determined to be of bacterial origin. A bacterial contaminant was isolated from the tissue culture plants and identified as a Bacillus pumilus. A set of sequence characterised amplified region (SCAR) primers were designed from the sequence of the cloned fragment and tested for the specific detection of B. pumilus. Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) were also used to generate B. pumilus profiles specific to our isolate in order to test and confirm the sequence homology of amplified markers generated from a range of DNA samples isolated from tissue culture plants and pure isolates of B. pumilus-like bacteria. 相似文献
14.
Light and electron microscopic techniques were employed to examine the kidneys of three species of pelagic seabird which had high tissue cadmium and mercury levels. Direct examination and comparison with the kidneys of cadmium and mercury dosed starlings showed that the seabirds had patchy nephrotoxic kidney lesions. It was suggested that these lesions were due to natural exposure of the animals to high levels of metals, particularly cadmium through their diet. Pathological features included necrosis and degeneration of the proximal tubular epithelium together with the direct observation of obstruction of the more distal neprhon segments by necrotic cellular debris. Abnormalities of the glomerular podocytes and Bowman's capsule cells were also observed. There was a high degree of similarity between the type and distribution of kidney lesions in the seabirds and the laboratory metal dosed starlings. The significance of this kidney damage and its possible effects on the birds survival prospects are discussed. 相似文献
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Phytoplankton abundance fluctuated throughout a 9 month study period in two 1.62 ha channel catfish (Ictalurus punctatus) production ponds. Summer phytoplankton communities in both ponds were dominated by blue-green algae (Cyanophyta). During
periods of rapidly decreasing phytoplankton abundance, total ammonia-nitrogen concentrations increased but a decrease in pH
during these same periods moderated the concentrations of unionized ammonia-nitrogen. Although unionized ammonia-nitrogen
concentrations did not reach levels considered acutely toxic to fish, extended periods of moderately high unionized ammonia-nitrogen
concentrations (>O.15 mg/1) occurred in the late fall months and may have serious implications for fish health. 相似文献
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18.
In early pregnancy, the O(2) gradient between the maternal circulation and the gestational sac tissues modulates trophoblast biological functions. The aim was to evaluate if placental partial pressure of oxygen (PaO(2)) modulates in vivo synthesis of specific placental proteins inside the first trimester gestational sac. Matched samples of peripheral venous blood, blood from the placental bed (PB), coelomic fluid (CF) and placental tissue were obtained in 37 normal pregnancies at 6-12 weeks gestation. PaO(2) was measured in PB and CF using an IRMA blood gas monitor. Inhibin A, activin A, sEng, PlGF, sFlt-1 and free VEGF concentrations were measured in all samples. HSP 70 was measured in placental extracts. ANOVA showed approximately 60% increase in PB PaO(2) (P = 0.02) between after 10 weeks gestation. Unpaired Student's T-test between two groups (6-9 weeks vs 9-12 weeks) shows a significant increase in MS Activin A (P = 0.001), CF activin A (P<0.001), MS P1GF (P = 0.001), CF PlGF (P<0.001), MS sFLT-1 (P = 0.03), CF sFLT-1 (P = 0.01), HSP 70 in placental extracts (P = 0.04) and a significant decrease in PB inhibin A levels (P<0.001) and PB sFLT-1 (P = 0.02) . Multiple correlation analysis showed a significant negative correlation between PB inhibin A levels and gestation (r = -0.45, P<0.05) and PB PaO(2) (r = -0.5, P = 0.008) and also between sFLT-1 and PB PaO(2) (P = 0.03). There was a positive correlation (P<0.01) between PlGF, sEng and VEGF levels in the placental extracts. Our results indicate a direct relationship in the early intrauterine PaO(2) in vivo and inhibin A and sFLT-1 concentrations confirming our hypothesis that specific placental proteins are regulated by intrauterine O(2) tension. 相似文献
19.
Introduction
While blood parasites are common in many birds in the wild, some groups seem to be much less affected. Seabirds, in particular, have often been reported free from blood parasites, even in the presence of potential vectors.Results
From a literature review of hemosporidian prevalence in seabirds, we collated a dataset of 60 species, in which at least 15 individuals had been examined. These data were included in phylogenetically controlled statistical analyses of hemosporidian prevalence in relation to ecological and life-history parameters. Haemoproteus parasites were common in frigatebirds and gulls, while Hepatozoon occurred in albatrosses and storm petrels, and Plasmodium mainly in penguins. The prevalence of Haemoproteus showed a geographical signal, being lower in species with distribution towards polar environments. Interspecific differences in Plasmodium prevalence were explained by variables that relate to the exposure to parasites, suggesting that prevalence is higher in burrow nesters with long fledgling periods. Measures of Plasmodium, but not Haemoproteus prevalences were influenced by the method, with PCR-based data resulting in higher prevalence estimates.Conclusions
Our analyses suggest that, as in other avian taxa, phylogenetic, ecological and life-history parameters determine the prevalence of hemosporidian parasites in seabirds. We discuss how these relationships should be further explored in future studies.20.
A C Beynen A H Terpstra 《Comparative biochemistry and physiology. B, Comparative biochemistry》1983,76(4):737-740
In humans, calves and rabbits very strong correlations were found between the concentrations of serum total cholesterol and phospholipids; in these species more than 70% of the variance in the level of serum phospholipids is accounted for by the variance in the serum cholesterol concentration. The regression coefficients in the equations relating the concentration of serum cholesterol to that of phospholipids were almost identical in humans and rabbits, but differed from those observed in calves and chickens. It is suggested that the rabbit is a suitable model animal for studying the relation between cholesterol and phospholipids in human serum. 相似文献