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Ertan Kucuksayan Aysegul Cort Mujgan Timur Evrim Ozdemir Suleyman Gultekin Yucel Prof. Dr. Tomris Ozben 《Journal of cellular biochemistry》2013,114(7):1685-1694
Antioxidants may prevent apoptosis of cancer cells via inhibiting reactive oxygen species (ROS). However, to date no study has been carried out to elucidate the effects of strong antioxidant N‐acetylcysteine (NAC) on Bleomycin induced apoptosis in human testicular cancer (NTERA‐2, NT2) cells. For this reason, we studied the effects of Bleomycin and NAC alone and in combination on apoptotic signaling pathways in NT2 cell line. We determined the cytotoxic effect of bleomycin on NT2 cells and measured apoptosis markers such as Caspase‐3, ‐8, ‐9 activities and Bcl‐2, Bax, Cyt‐c, Annexin V‐FTIC and PI levels in NT2 cells incubated with different agents for 24 h. Early apoptosis was determined using FACS assay. We found half of the lethal dose (LD50) of Bleomycin on NT2 cell viability as 400, 100, and 20 µg/ml after incubations for 24, 48, and 72 h, respectively. Incubation with bleomycin (LD50) and H2O2 for 24 h increased Caspase‐3, ‐8, ‐9 activities, Cyt‐c and Bax levels and decreased Bcl‐2 levels. The concurrent incubation of NT2 cells with bleomycin/H2O2 and NAC (5 mM) for 24 h abolished bleomycin/H2O2‐dependent increases in Caspase‐3, ‐8, ‐9 activities, Bax and Cyt‐c levels and bleomycin/H2O2‐dependent decrease in Bcl‐2 level. Our results indicate that bleomycin/H2O2 induce apoptosis in NT2 cells by activating mitochondrial pathway of apoptosis, while NAC diminishes bleomycin/H2O2 induced apoptosis. We conclude that NAC has antagonistic effects on Bleomycin‐induced apoptosis in NT2 cells and causes resistance to apoptosis which is not a desired effect in eliminating cancer cells. J. Cell. Biochem. 114: 1685–1694, 2013. © 2013 Wiley Periodicals, Inc. 相似文献
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Anti‐Inflammatory and Anti‐Oxidant Effects of p‐Chloro‐phenyl‐selenoesterol on TNBS‐Induced Inflammatory Bowel Disease in Mice
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Micheli Stéfani Zarzecki Vandreza Cardoso Bortolotto Márcia Rósula Poetini Stífani Machado Araujo Mariane Trindade de Paula Silvane Souza Roman Cristiano Spiazzi Franciele Weber Santos Cibin Oscar Endrigo Dorneles Rodrigues Cristiano Ricardo Jesse Marina Prigol 《Journal of cellular biochemistry》2017,118(4):709-717
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Krishnaveni Mohareer Sudhir Sahdev Seyed E. Hasnain 《Journal of cellular biochemistry》2013,114(4):899-907
p53 protein, the central molecule of the apoptosis pathway, is mutated in 50% of the human cancers. Of late, p53 homologues have been identified from different invertebrates including Drosophila melanogaster, Caenorhabditis elegans, Squid, and Clams. We report the identification of a p53‐like protein in Spodoptera frugiperda (Sf9) insect cells, which is activated during oxidative stress, caused by exposure to UV‐B or H2O2, and binds to p53 consensus DNA binding motifs as well as other p53 cognate motifs. Sf9 p53 motif‐binding protein is similar to murine and Drosophila p53 in terms of molecular size, which is around 50–60 kDa, as evident from UV cross‐linking, and displays DNA binding characteristics similar to both insect and vertebrate p53 as seen from electrophoretic mobility shift assays. The N‐terminal sequencing of the purified Sf9 p53 motif‐binding protein reveals extensive homology to the pro‐apoptotic FK‐506 binding protein (FKBP‐46), earlier identified in Sf9 cells as a factor which interacts with murine casein kinase. FKBP, an evolutionarily conserved protein of mammalian origin functions as a pro‐apoptotic factor. Identification of FKBP‐46 as a novel p53 motif‐binding protein in insect cells adds a new facet to our understanding of the mechanisms of apoptosis under oxidative stress in the absence of a typical p53 homologue. J. Cell. Biochem. 114: 899–907, 2013. © 2012 Wiley Periodicals, Inc. 相似文献
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Annamaria Cimini Roberta Gentile Francesco Angelucci Elisabetta Benedetti Giuseppina Pitari Antonio Giordano Rodolfo Ippoliti 《Journal of cellular biochemistry》2013,114(3):708-715
Peroxiredoxins are ubiquitous proteins that recently attracted major interests in view of the strict correlation observed in several cell lines and/or tissues between different levels of their expression and the increased capacity of cells to survive in different pathophysiological conditions. They are recently considered as the most important enzymes regulating the concentration of hydroperoxides inside the cells. Most of neurodisorders such as Parkinson, Huntington, Alzheimer's diseases, and ischemic injury are characterized by conditions of oxidative stress inside cells. In these pathophysiological conditions, a strict correlation between cell survival and Prx expression has been found. In CNS all the Prx isoforms are present though with different expression pattern depending on cell phenotype. Interestingly, neurons treated with amyloid beta peptide (Aβ), showed an overexpression of PrxI. In this study, the neuroprotective effect of PrxI after Aβ exposure and the underlying mechanisms by which PrxI expression counteracts cell death was investigated in a well established human AD in vitro model. Taking advantage on cells transfected by a construct where human PrxI is fused with a Green fluorescent protein (GFP) at the C‐terminus, we report some events at the basis of cell survival after Aβ injury, suggesting possible new signal cascades dealing with the antiapoptotic effect of PrxI. The results obtained indicated a protective role for PrxI in counteracting Aβ injury by increasing cell viability, preserving neurites, and decreasing cell death. J. Cell. Biochem. 114: 708–715, 2013. © 2012 Wiley Periodicals, Inc. 相似文献
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Recombinant Mitochondrial Manganese Containing Superoxide Dismutase Protects Against Ochratoxin A‐Induced Nephrotoxicity
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Roberto Ciarcia Sara Damiano Caterina Squillacioti Nicola Mirabella Ugo Pagnini Alessia Florio Lorella Severino Giovambattista Capasso Antonella Borrelli Aldo Mancini Silvia Boffo Gaetano Romano Antonio Giordano Salvatore Florio 《Journal of cellular biochemistry》2016,117(6):1352-1358
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Resveratrol Ameliorates Palmitate‐Induced Inflammation in Skeletal Muscle Cells by Attenuating Oxidative Stress and JNK/NF‐κB Pathway in a SIRT1‐Independent Mechanism
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Asie Sadeghi Shadi Sadat Seyyed Ebrahimi Abolfazl Golestani Reza Meshkani 《Journal of cellular biochemistry》2017,118(9):2654-2663
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Anti‐Inflammatory and Antioxidant Actions of Copaiba Oil Are Related to Liver Cell Modifications in Arthritic Rats
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Cristiane V. Castro Ghizoni Ana P. Arssufi Ames Osmar A. Lameira Ciomar A. Bersani Amado Anacharis B. Sá Nakanishi Lívia Bracht Maria R. Marçal Natali Rosane M. Peralta Adelar Bracht Jurandir F. Comar 《Journal of cellular biochemistry》2017,118(10):3409-3423
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