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1.
Serological identification and molecular characterization of B (A) 02 subtype
in patients and blood donors from Eastern China
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Wen Wu Yecui Zhang Xingying Guo Peng Wu Zhibo Zhang Xiangyan Huang 《Asia-Pacific Journal of Blood Types and Genes》2017,1(1):55-59
This study was designed to identify the rare?type?ABO?blood?groups, B(A) 02, from Eastern China. Three samples with discordant serological results during routine blood type identification and four samples from one sample’ family were selected. All of them were detected by serological method. The exon 6 and 7 of the ABO genes were amplified by PCR and sequenced. They were typed as AsubB by serology and as BO by genotype. In AsubB samples, nt 700C>G mutation was detected in B gene, which was previously defined as B(A)02 alleles. In these seven samples, six showed B(A)02/O01 and one showed B(A)02/O02.B(A)02 allele was found to be more common in this study than B(A)04 which is considered to be more frequent than B(A)02. The careful identification of rare blood types is important for the safety of clinical blood transfusion. 相似文献
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Björklund M Ranta E Kaitala V Bach LA Lundberg P 《Bulletin of mathematical biology》2011,73(7):1666-1681
The probability of, and time to, fixation of a mutation in a population has traditionally been studied by the classic Wright–Fisher
model where population size is constant. Recent theoretical expansions have covered fluctuating populations in various ways
but have not incorporated models of how the environment fluctuates in combination with different levels of density-compensation
affecting fecundity. We tested the hypothesis that the probability of, and time to, fixation of neutral, advantageous and
deleterious mutations is dependent on how the environment fluctuates over time, and on the level of density-compensation.
We found that fixation probabilities and times were dependent on the pattern of autocorrelation of carrying capacity over
time and interacted with density-compensation. The pattern found was most pronounced at small population sizes. The patterns
differed greatly depending on whether the mutation was neutral, advantageous, or disadvantageous. The results indicate that
the degree of mismatch between carrying capacity and population size is a key factor, rather than population size per se,
and that effective population sizes can be very low also when the census population size is far above the carrying capacity.
This study highlights the need for explicit population dynamic models and models for environmental fluctuations for the understanding
of the dynamics of genes in populations. 相似文献
3.
Miao H Dykes C Demeter LM Cavenaugh J Park SY Perelson AS Wu H 《Bulletin of mathematical biology》2008,70(6):1749-1771
Growth competition assays have been developed to quantify the relative fitness of HIV-1 mutants. In this article, we develop
mathematical models to describe viral/cellular dynamic interactions in the assay system from which the competitive fitness
indices or parameters are defined. In our previous HIV-viral fitness experiments, the concentration of uninfected target cells
was assumed to be constant (Wu et al. 2006). But this may not be true in some experiments. In addition, dual infection may frequently occur in viral fitness experiments
and may not be ignorable. Here, we relax these two assumptions and extend our earlier viral fitness model (Wu et al. 2006). The resulting models then become nonlinear ODE systems for which closed-form solutions are not achievable. In the new model,
the viral relative fitness is a function of time since it depends on the target cell concentration. First, we studied the
structure identifiability of the nonlinear ODE models. The identifiability analysis showed that all parameters in the proposed
models are identifiable from the flow-cytometry-based experimental data that we collected. We then employed a global optimization
approach (the differential evolution algorithm) to directly estimate the kinetic parameters as well as the relative fitness
index in the nonlinear ODE models using nonlinear least square regression based on the experimental data. Practical identifiability
was investigated via Monte Carlo simulations. 相似文献
4.
Willem H. Koppenol 《Journal of biological inorganic chemistry》2013,18(7):865-866
Wegerich et al. (J. Biol. Inorg. Chem. 18:429–440, 2013), working with singly modified human cytochromes c, claim to have found a new mechanism for the reduction of iron(III) cytochrome c by superoxide. I show that electron transfer by way of the solvent-accessible haem edge—a mechanism not considered by Wegerich et al.—is still the correct mechanism. Furthermore, several deficiencies in this work preclude any comparisons with other publications on this topic. 相似文献
5.
Haensch KT 《Plant cell reports》2004,23(4):211-217
The possibility of inducing somatic embryogenesis in petiole cultures of two cultivars of Pelargonium × hortorum and of one cultivar of Pelargonium × domesticum using thidiazuron (TDZ) was investigated. Petioles were cultivated on a modified Murashige and Skoog medium with different concentrations and application periods of TDZ. Regeneration was achieved with all TDZ treatments for all cultivars and was highly variable. Shoots of different shapes and somatic embryo-like structures were observed. Histological examination revealed that no somatic embryos were formed, and regenerants had to be classified as shoots and shoot-like or leaf-like structures. The importance of these results on the classification of regeneration induced by TDZ in these species and on the propagation of these pelargoniums is discussed. 相似文献
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Andreasen V 《Bulletin of mathematical biology》2011,73(10):2305-2321
We study the final size equation for an epidemic in a subdivided population with general mixing patterns among subgroups.
The equation is determined by a matrix with the same spectrum as the next generation matrix and it exhibits a threshold controlled
by the common dominant eigenvalue, the basic reproduction number R0{\mathcal{R}_{0}}: There is a unique positive solution giving the size of the epidemic if and only if R0{\mathcal{R}_{0}} exceeds unity. When mixing heterogeneities arise only from variation in contact rates and proportionate mixing, the final
size of the epidemic in a heterogeneously mixing population is always smaller than that in a homogeneously mixing population
with the same basic reproduction number R0{\mathcal{R}_{0}}. For other mixing patterns, the relation may be reversed. 相似文献
8.
Christopher D. Gregory John D. Pound 《Apoptosis : an international journal on programmed cell death》2010,15(9):1029-1049
The apoptosis program of physiological cell death elicits a range of non-phlogistic homeostatic mechanisms—“recognition, response and removal”—that regulate the microenvironments of normal and diseased tissues via multiple modalities operating over short and long distances. The molecular mechanisms mediate intercellular signaling through direct contact with neighboring cells, release of soluble factors and production of membrane-delimited fragments (apoptotic bodies, blebs and microparticles) that allow for interaction with host cells over long distances. These processes effect the selective recruitment of mononuclear phagocytes and the specific activation of both phagocytic and non-phagocytic cells. While much evidence is available concerning the mechanisms underlying the recognition and responses of phagocytes that culminate in the engulfment and removal of apoptotic cell bodies, relatively little is yet known about the non-phagocytic cellular responses to the apoptosis program. These responses regulate inflammatory and immune cell activation as well as cell fate decisions of proliferation, differentiation and death. Here, we review current knowledge of these processes, considering especially how apoptotic cells condition the microenvironments of normal and malignant tissues. We also discuss how apoptotic cells that persist in the absence of phagocytic clearance exert inhibitory effects over their viable neighbors, paying particular attention to the specific case of cell cultures and highlighting how new cell-corpse-clearance devices—Dead-Cert® Nanoparticles—can significantly improve the efficacy of cell cultures through effective removal of non-viable cells in the absence of phagocytes in vitro. 相似文献
9.
The analysis of data usage in a large set of real traces from a high-energy physics collaboration revealed the existence of
an emergent grouping of files that we coined “filecules”. This paper presents the benefits of using this file grouping for
prestaging data and compares it with previously proposed file grouping techniques along a range of performance metrics. Our
experiments with real workloads demonstrate that filecule grouping is a reliable and useful abstraction for data management
in science Grids; that preserving time locality for data prestaging is highly recommended; that job reordering with respect
to data availability has significant impact on throughput; and finally, that a relatively short history of traces is a good
predictor for filecule grouping. Our experimental results provide lessons for workload modeling and suggest design guidelines
for data management in data-intensive resource-sharing environments.
相似文献
Gabriele GarzoglioEmail: |
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Bifidobacterium adolescentis Int-57 (INT57), isolated from human feces, secretes an amylase. We have shot-gun cloned, sequence analyzed and expressed
the gene encoding this amylase in B. longum. The sequenced 2477 bp fragment was homologous to other extracellular amylases. The encoded protein was predicted to be composed
of 595 amino acids with a molecular weight of 64 kDa, and was designated AmyB. Highly conserved amylase domains were found
in AmyB. The signal sequence and cleavage site was predicted by sequence analysis. AmyB was subcloned into pBES2, a novel E. coli–Bifidobacterium shuttle vector, to construct pYBamy59. Subsequently, B. longum, with no apparent amylase activity, was transformed with pYBamy59. More than 90% of the amylase activity was detected in
the culture broth. This approach may open the way for the development of more efficient expression and secretion systems for
Bifidobacterium.
Both authors contributed equally
Received 17 June 2005; Revisions requested 13 July 2005 and 26 September 2005; Revisions received 12 September 2005 and 8
November 2005; Accepted 11 November 2005 相似文献
13.
MLIV (mucolipidosis type?IV) is a neurodegenerative lysosomal storage disorder caused by mutations in MCOLN1, a gene that encodes TRPML1 (mucolipin-1), a member of the TRPML (transient receptor potential mucolipin) cation channels. Two additional homologues are TRPML2 and TRPML3 comprising the TRPML subgroup in the TRP superfamily. The three proteins play apparently key roles along the endocytosis process, and thus their cellular localization varies among the different group members. Thus TRPML1 is localized exclusively to late endosomes and lysosomes, TRPML2 is primarily located in the recycling clathrin-independent GPI (glycosylphosphatidylinositol)-anchored proteins and early endosomes, and TRPML3 is primarily located in early endosomes. Apparently, all three proteins' main physiological function underlies Ca(2+) channelling, regulating the endocytosis process. Recent findings also indicate that the three TRPML proteins form heteromeric complexes at least in some of their cellular content. The physiological role of these complexes in lysosomal function remains to be elucidated, as well as their effect on the pathophysiology of MLIV. Another open question is whether any one of the TRPMLs bears additional function in channel activity. 相似文献
14.
Arguments are presented for the merit of integrating specimen databases into the practice of revisionary systematics. Work flows, data connections, data outputs, and data standardization are enumerated as critical aspects of such integration. Background information is provided on the use of "barcodes" as unique specimen identifiers and on methods for efficient data capture. Examples are provided on how to achieve efficient workflows and data standardization, as well as data outputs and data integration. 相似文献
15.
Albumin, the major circulating protein in blood, can undergo increased glycation in diabetes. One of the main properties of this plasma protein is its strong affinity to bind many therapeutic drugs, including warfarin and ketoprofen. In this study, we investigated whether or not there were any significant changes related to in vitro or in vivo glycation in the structural properties and the binding of human albumin to both therapeutic drugs. Structural parameters, including redox state and ketoamine contents of in vitro and in vivo glycated purified albumins, were investigated in parallel with their affinity for warfarin and ketoprofen. High-performance liquid chromatography was used to determine the free drug concentrations and dissociation constants according to the Scatchard method. An alternative method based on fluorescence spectroscopy was also used to assess drug-binding properties. Oxidation and glycation levels were found to be enhanced in albumin purified from diabetic patients or glycated with glucose or methylglyoxal, after determination of their ketoamine, free thiol, amino group and carbonyl contents. In parallel, significant impairments in the binding affinity of in vitro and in vivo glycated albumin, as indicated by the higher dissociation constant values and confirmed by higher free drug fractions, were observed. To a lesser extent, this alteration also significantly affected diabetic albumin affinity, indicated by a lower static quenching in fluorescence spectroscopy. This work provides useful information supporting in vivo diabetic albumin could be the best model of glycation for monitoring diabetic physiopathology and should be valuable to know if glycation of albumin could contribute to variability in drugs response during diabetes. 相似文献
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Many studies of the evolution of life-history traits assume that the underlying population dynamical attractor is stable point
equilibrium. However, evolutionary outcomes can change significantly in different circumstances. We present an analysis based
on adaptive dynamics of a discrete-time demographic model involving a trade-off whose shape is also an important determinant
of evolutionary behaviour. We derive an explicit expression for the fitness in the cyclic region and consequently present
an adaptive dynamic analysis which is algebraic. We do this fully in the region of 2-cycles and (using a symbolic package)
almost fully for 4-cycles. Simulations illustrate and verify our results. With equilibrium population dynamics, trade-offs
with accelerating costs produce a continuously stable strategy (CSS) whereas trade-offs with decelerating costs produce a
non-ES repellor. The transition to 2-cycles produces a discontinuous change: the appearance of an intermediate region in which
branching points occur. The size of this region decreases as we move through the region of 2-cycles. There is a further discontinuous
fall in the size of the branching region during the transition to 4-cycles. We extend our results numerically and with simulations
to higher-period cycles and chaos. Simulations show that chaotic population dynamics can evolve from equilibrium and vice-versa. 相似文献
18.
Kannan Rama Devi Ramanathan Srinivasan Arunachalam Kannappan Sivasubramanian Santhakumari Murugan Bhuvaneswari Periyannan Rajasekar 《Biofouling》2016,32(10):1171-1183
Rosmarinic acid (RA) was assessed for its quorum sensing inhibitory (QSI) potential against Aeromonas hydrophila strains AH 1, AH 12 and MTCC 1739. The pathogenic strains of A. hydrophila were isolated from infected zebrafish and identified through biochemical analysis and amplification of a species-specific gene (rpsL). The biofilm inhibitory concentration (BIC) of RA against A. hydrophila strains was found to be 750 μg ml?1. At this concentration, RA reduced the QS mediated hemolysin, lipase and elastase production in A. hydrophila. In FT-IR analysis, RA treated A. hydrophila cells showed a reduction in cellular components. Gene expression analysis confirmed the down-regulation of virulence genes such as ahh1, aerA, lip and ahyB. A. hydrophila infected zebrafish upon treatment with RA showed increased survival rates. Thus, the present study demonstrates the use of RA as a plausible phytotherapeutic compound to control QS mediated biofilm formation and virulence factor production in A. hydrophila. 相似文献
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Nerve conduits filled with longitudinal aligned filaments have demonstrated a better regenerative outcome for bridging large
peripheral nerve gaps than hollow nerve conduits. In the present study, we investigated the in vitro and in vitro cellular
behavior of Schwann cells on polyglycolic acid (PGA) filaments by immunocyto/histochemistry and light/electron microscopy.
After 1-3-week culture of rat dorsal root ganglia (DRGs) onto PGA filaments, Schwann cells from rat DRGs adhered to and migrated
along PGA filaments. Twenty-four rats received implantation of chitosan conduits inserted with PGA filaments to bridge 10-mm-long
sciatic nerve gaps. At 1, 2, 3 and 4 weeks post-implantation (n = 6, each time point), Schwann cells were found to migrate
along PGA filaments and form cell columns resembling bands of Büngner. These results suggest that PGA filaments may play a
contact guidance role in Schwann cell migration and thus serve as a promising conduit-filling material to facilitate peripheral
nerve repair. 相似文献