Coccidian Parasites (Apicomplexa: Eimeriidae) from Insectivores. II. Six New Species from Japanese Shrew Moles (Talpidae)1

ABSTRACT. Thirty-eight of 51 (74.5%) shrew moles collected in Japan were infected with from one to four species of Eimeria and/or Isospora including six of six Dymecodon pilirostris and 32 of 45 (71.1%) Urotrichus talpoides. Four eimerians and two isosporans were identified and all are described as new species. Sporulated oocysts of Eimeria amorphospora n. sp. were subspheroid/ellipsoid, 21.1 × 17.9(18-25 × 16-21) μm. Sporocysts were amorphous, gelatinoid envelopes 20.3 × 7.5 (17–24 × 7–9) μm. Sporozoites were enclosed together within a membrane in each sporocyst. This species was found in 9 of 45 (20%) U. talpoides. Sporulated oocysts of Eimeria gonocilia n. sp. were subspheroid/ellipsoid, 28.8 × 24.4 (25–30 × 21–28) μm; a highly ornate outer oocyst wall gave the appearance of a ciliated ball. Sporocysts ovoid, pointed at both ends, were 17.0 × 9.0 (15–19 × 7–11) μm; this species was found in 4 of 45 (8.9%) U. talpoides. Sporulated oocysts of Eimeria talpoidei n. sp. were asymmetrical ovoid, 20.6 × 13.3 (18–23 × 12–15) μm, with sporocysts lacrimiform, 12.0 × 5.8 (10–14 × 5–7) μm. This species was found in 7 of 45 (15.6%) U. talpoides. Sporulated oocysts of Eimeria honshuensis n. sp. were ellipsoid, 15.5 × 11.4 (13–18 × 10–13) μm, with sporocysts ovoid, 9.1 × 5.2 (8–10 × 4–6) μm. This species was found in 10 of 45 (22.2%) U. talpoides and in 5 of 6 (83.3%) D. pilirostris. Sporulated oocysts of Isospora dymecodi n. sp. were subspheroid/ellipsoid, 15.8 × 12.6 (13–17 × 11–13) μm, with sporocysts ellipsoid, 10.9 × 6.9 (10–13 × 6–8). This species was found in six of six D. pilirostris. Sporulated oocysts of Isospora urotrichi n. sp. were spheroid/subspheroid, 13.4 × 12.4 (11–16 × 9–14) μm, with sporocysts ovoid, 9.2 × 6.3 (8–11 × 5–7) μm. This species was found in 27 of 45 (60%) U. talpoides. Only 14 of 38 (36.8%) infected hosts (one D. pilirostris, 13 U. talpoides) were seen to be naturally infected with only one coccidian species when sampled.     

Coccidian Parasites (Apicomplexa: Eimeriidae) from Insectivores. VI. Six New Species from the Eastern Mole,Scalopus aquaticus1

ABSTRACT Thirteen eastern moles, Scalopus aquaticus, collected in West Texas were examined for coccidian oocysts; 11 (85%) were infected and eight (73%) of these had multiple infections representing two or more species. One cyclosporan, three eimerians, and two isosporans were studied and all are described as new species. Sporulated oocysts of Cyclospora megacephali n. sp. were subspheroidal, 18.5 × 15.7 (14–21 × 12–18) μm; they had sporocysts pointed at one end with Stieda bodies nearly as wide as the sporocysts themselves, and were 15.0 × 7.2 (11–17 × 6–9) μm; C. megacephali was found in four (31%) hosts. Sporulated oocysts of Eimeria scalopi n. sp. were spheroidal to subspheroidal, 13.6 × 12.6 (11–17 × 11–15) μm with sporocysts lemon-shaped, 8.7 × 5.5 (7–10 × 4–7) μm; it was found in six (46%) hosts. Sporulated oocysts of Eimeria aquatici n. sp. were asymmetrically ellipsoidal, 17.0 × 10.6 (14–20 × 9–14) μm, with sporocysts elongately ovoidal, 9.0 × 5.2 (8–11 × 4–6) μm; it was found in two (15%) hosts. Sporulated oocysts of Eimeria motleiensis n. sp. were subspheroidal, 17.0 × 15.3 (15–20 × 13–18) μm with sporocysts ovoidal, 10.7 × 6.8 (10–13 × 6–8) μm; it was found in seven (54%) hosts. Sporulated oocysts of Isospora motleiensis n. sp. were spheroidal to subspheroidal, 13.6 × 12.0 (10–17 × 8–15) μm with sporocysts broadly ovoidal, 9.5 × 6.7 (7–11 × 4–8) μm; it was found in nine (69%) hosts. Sporulated oocysts of Isospora aquatici n. sp. were subspheroidal, 20.9 × 18.4 (15–24 × 13–21) μm with sporocysts ellipsoidal, 11.8 × 9.0 (9–14 × 7–11) μm; it was found in two (15%) hosts.     

Coccidian Parasites (Apicomplexa: Eimeriidae) from Insectivores. IV. Four New Species in Talpa europaea from England1

ABSTRACT. Moles from England were examined for coccidian oocysts and all 64 Talpa europaea were infected; of 64 infected hosts, 56 (88%) had multiple infections representing two to six coccidian species when examined. Oocysts in 31 of the 64 samples remained unsporulated. Three eimerians and one isosporan were studied from the 33 fecal samples that had sporulated oocysts and these are described as new species; Cyclospora talpae Pellérdy & Tanyi, 1968, and Isospora sofiae (Golemansky, 1978) Levine & Ivens, 1979, are redescribed; and Cyclospora sp., similar to C. talpae, is discussed. Sporulated oocysts of C. talpae are ellipsoidal, 14.3 × 9.6 (12–19 × 6–13) μm with sporocysts ovoid, 9.4 × 5.7 (6–13 × 4–8) μm; it was found in 21 of the 33 (63.6%) sporulated samples. Sporulated oocysts of Cyclospora sp. are subspheroidal to ellipsoidal, 12.5 × 8.9 (10–14 × 6–12) μm with sporocysts ovoid, 8.6 × 5.3 (6–10 × 4–6) μm; it was found in 21 of the 33 (63.6%) sporulated samples. Sporulated oocysts of Eimeria avonensis n. sp. are elongate-ellipsoidal, 15.0 × 9.6 (13–20 × 7–12) μm with sporocysts ovoid, 6.6 × 3.6 (5–9 × 3–7) μm; it was found in 15 of the 33 (45.5%) sporulated samples. Sporulated oocysts of Eimeria berea n. sp. are subspheroidal, 12.1 × 10.5 (10–15 × 8–14) μm with sporocysts ovoid, 6.3 × 3.9 (5–10 × 2–5) μm; it was found in 8 of the 33 (24.2%) sporulated samples. Sporulated oocysts of Eimeria globula n. sp. are spheroidal, 20.9 × 19.9 (19–24 × 17–21) μm with sporocysts elongate-ovoid, 11.5 × 6.9 (9–16 × 6–9) μm; it was found in 3 of the 33 (9.1%) sporulated samples. Sporulated oocysts of Isospora sporopointaea n. sp. are subellipsoidal to ellipsoidal, 17.1 × 11.4 (13–21 × 8–14) μm with sporocysts ellipsoidal with both ends pointed, 11.9 × 5.9 (9–16 × 4–8) μm; it was found in 27 of the 33 (81.8%) sporulated samples. Sporulated oocysts of I. sofiae are spheroidal to subspheroidal, 12.2 × 11.0 (9–16 × 8–15) μm with sporocysts ovoid, 9.1 × 5.2 (6–13 × 3–8) μm; it was found in 25 of the 33 (75.8%) sporulated samples. To date, the coccidian parasites of talpids include two cyclosporans, 12 eimerians, and six isosporans, exclusive of the four new species described here.     

Eimeria beldingii n. sp. and Other Coccidia (Apicomplexa) of the Ground Squirrel Spermophilus beldingi1

Fecal samples of 36 ground squirrels, Spermophilus beldingi, from Tioga Pass (elev. ca. 3315 m) in the Sierra Nevada, California, yielded oocysts of Eimeria beckeri in nine squirrels, E. citelli in four squirrels, E. beldingii n. sp. in two squirrels, and degenerated, unidentifiable oocysts in ten squirrels. Eimeria beldingii n. sp. oocysts are ellipsoidal, 30–34 × 24–30 (mean 32 × 26) μm with a two-layered, rough, striated wall, without a micropyle or residuum, with polar granules; they contain ellipsoidal or ovoid sporocysts 11–15 × 9–12 (mean 13 × 10) μm with a Stieda body and residuum.     

Eimeria ladronensis n. sp. and E. albigulae (Apicomplexa: Eimeriidae) from the Woodrat,Neotoma albigula (Rodentia: Cricetidae)1

Of 50 white-throated woodrats (Neotoma albigula) collected from Socorro Co., New Mexico, 21 (42%) had eimerian oocysts in their feces when examined. Of the 21 Neotoma found positive for Eimeria, 19 (90%) harbored a single eimerian species at time of examination. Eimeria albigulae Levine, Ivens & Kruidenier, 1957, was found in 18 (86%), and E. ladronensis n. sp. was found in five (24%) infected woodrats. Sporulated oocysts of E. ladronensis are ellipsoidal, 19–25 × 13–15 (21.4 ± 1.3 × 14.1 ± 1.1) μm, have a smooth wall and one or two polar granules, but lack a micropyle and an oocyst residuum. Sporocysts are tapered at one end, 7–10 × 6–7 (8.5 ± 0.7 × 6.5 ± 0.3) μm, and have a Stieda body and sporocyst residuum, but no substieda body. Prepatent periods for E. albigulae and E. ladronensis n. sp. are 5–6 and 8–9 days, respectively; patent periods are 7–18 and approximately 11 days, respectively.     

Eimeria tahamensis N. Sp. (Apicomplexa: Eimeriidae) from the Arabian Quail (Coturnix delegorguei arabica)1

Eimeria tahamensis n. sp. is described from the harlequin quail (Coturnix delegorguei arabica) from Tahama, Saudi Arabia. The sporulated oocysts of E. tahamensis n. sp. are ellipsoid, 36.5–42 × 25.5–29 (41.2 ± 1.34 × 28.4 ± 0.81) μm, with a thick two-layered wall and one polar granule but without a micropyle or an oocyst residuum. The sporocysts are ovoid, 14–16 × 9–11.5 (15.3 ± 0.7 × 10.8 ± 0.64) μm, with a knob-like Stieda body and sporocyst residuum, but without a substiedal body. The sporozoites are often located transversely at the two ends of the sporocysts. The host bird belongs to the order Galliformes.     

Two New Species of Eimeria from Peacocks (Pavo cristatus) in Saudi Arabia1

Fifteen fecal samples from peacocks (Pavo cristatus) in Saudi Arabia contained oocysts of Eimeria riyadhae n. sp. in two peacocks and oocysts of E. arabica n. sp. in one peacock. Sporulated oocysts of Eimeria riyadhae are ellipsoidal, 27–30.5 times 20.5–25 (28.8 ± 1.3 × 22.4 ± 1.6) μm, with a two-layered wall and bilobed polar body, but without a micropyle or residuum. The sporocysts are ovoid, 11–14.5 × 6.5–8 (13.2 ± 1.2 × 7.2 ± 0.6) μm with a thick, knob-like Stieda body and a residuum. Sporulated oocysts of Eimena arabica are spheroidal, 17.5–21.5 × 17.5–21.5 (19.2 ± 1.6 × 19.2 ± 1.6) μm, with a two-layered wall and two refractile polar bodies, but without a micropyle or residuum. The sporocysts are elongate ovoid, 9.5–12 × 4–6.5 (11.2 ± 0.9 × 5.5 ± 0.88), with a small crescent-shaped Stieda body. The host bird belongs to the order Galliformis.     

Coccidia of Brazilian Mammals: Eimeria corticulata N. Sp. (Apicomplexa: Eimeriidae) from the Anteater Tamandua tetradactyla (Xenarthra: Myrmecophagidae) and Eimeria zygodontomyis N. Sp. from the Cane Mouse Zygodontomys lasiurus (Rodentia: Cricetidae)

Feces from a specimen of Tamandua tetradactyla (Linn.) from Portel, Para State, north Brazil, contained two different coccidial oocysts; one identified as Eimeria tamanduae Lainson 1968, and the other as a new species, described here as Eimeria corticulata n. sp. Oocysts of E. corticulata are ellipsoidal, 37.4 × 30.4 (31.2–43.7 × 23.7–35.0) μm, shape index (length/width) 1.2 (1.0–1.5). Oocyst wall 2.5–3.7 μm thick and composed of two layers; an outer thick, brown-yellow one with radial striations, and a thin inner smooth one: no visible micropyle. Oocyst residuum a large globule of about 10.7 × 10.3 μm, usually accompanied by a number of smaller attached globules. Sporocysts ellipsoidal, 21.0 × 11.0 (20.0–22.5 × 10.0–12.5) μm, with a conspicuous Stieda body: shape index 1.9 (1.6–2.2). Sporocyst residuum a small number of scattered granules: sporozoites 18.7 × 5.0 μm, with a large posterior refractile body. Eimeria zygodontomyis n. sp. is described in feces from Zygodontomys lasiurus (Lund) from the Serra dos Carajas, Para. Oocysts ellipsoidal to cylindrical, 16.5 × 12.0 (13.7–18.7 × 11.2–12.3) μm, shape index 1.4 (1.2–1.5). Wall colorless, smooth, single-layered and about 0.6 μm thick: no micropyle. No oocyst residuum, but a polar granule of about 1.8 × 1.0 μm is sometimes present. Sporocysts ellipsoidal, 8.4 × 5.5(7.5–8.7 × 5.0–6.2) μm, shape index 1.5 (1.4–1.7), with a thin colorless wall and a delicate Stieda body. Sporozoites enclose a compact residuum of about 2.5 × 3.7 μm.     

Coccidia (Apicomplexa: Eimeriidae) from Sciurid Rodents (Eutamias,Sciurus, Tamiasciurus spp.) from the Western United States and Northern Mexico with Descriptions of Two New Species1

ABSTRACT. Since May 1979, 190 rodents in the family Sciuridae, representing three genera and nine species, have been collected in the western United States and northern Mexico and examined for coccidia; 71 (37%) had coccidian oocysts in their feces. These included 2 of 12 (17%) Eutamias canipes; 7 of 12 (58%) E. dorsalis; 18 of 50 (36%) E. merriami; 33 of 96 (34%) E. obscurus; 3 of 4 (75%) E. townsendii; 3 of 9 (33%) Sciurus aberti; 1 of 1 S. griseus; 1 of 1 Tamiasciurus hudsonicus mogollonensis; and 3 of 5 (60%) T. mearnsi. The following coccidians were identified from infected rodents: Eimeria cochisensis n. sp. and Eimeria dorsalis n. sp. from E. canipes; E. cochisensis, E. dorsalis, and E. tamiasciuri from E. dorsalis; E. dorsalis and E. tamiasciuri from E. merriami; E. cochisensis, E. dorsalis, E. tamiasciuri, and E. wisconsinensis from E. obscurus; E. cochisensis and E. dorsalis from E. townsendii; E. ontarioensis and E. tamiasciuri from S. aberti; E. tamiasciuri from S. griseus; E. tamiasciuri and E. toddi from T. h. mogollonensis; and E. tamiasciuri from T. mearnsi. Sporulated oocysts of Eimeria dorsalis n. sp. were ovoid, 21.9 × 16.8 (17–24 × 14–20) μm with sporocysts ovoid, 11.5 × 6.9 (10–14 × 6–8) μm. Sporulated oocysts of Eimeria cochisensis n. sp. were spheroid to subspheroid, 16.7 × 15.3 (15–18 × 14–17) μm, with sporocysts ovoid, 8.4 × 5.6 (6–11 × 4–7) μm. Fifty-five of 71 (77%) infected hosts had oocysts of only one eimerian species in their feces at the time they were examined. One eimerian, E. tamiasciuri, was found in seven of nine host species in three genera. A list is provided of all eimerians (22, including the species described here) that have been described in the literature from Eutamias, Sciurus, and Tamiasciurus spp.     

Ascogregarina saraviae n. sp. (Apicomplexa: Lecudinidae) in Lutzomyia lichyi (Diptera: Psychodidae)

Ascogregarina saraviae n. sp. (Apicomplexa: Lecudinidae) is described from wild-caught Lutzomyia lichyi (Diptera: Psychodidae) females. Gametocysts adhered to the hemocoel side of the genital accessory gland walls and oocysts were injected into their lumina. Sporulated oocysts were ellipsoidal, 12.4 x 5.8 (11.6-13.1 x 5.6-5.9) micrometers, contained eight sporozoites and a refractile residuum. The elongate form of A. saraviae n. sp. oocysts, and their more delicate walls, clearly distinguish them from oocysts of A. chagasi (Adler & Mayrink, 1961).     

Two New Species of Isospora from Indonesian Birds1

ABSTRACT. The following species are described from Indonesian birds: Isospora paddae n. sp. with oocysts 41.5–45.5 × 40.3–41.5 (44 ± 1.15 × 41.2 ± 0.38) and sporocysts 22.8–24.5 × 14.7–17 (24 ± 0.55 × 16.2 ± 0.81) from the Java sparrow, Padda oryzivora, and Isospora indonesianensis n. sp. with oocysts 39.3–43.6 × 37–40.8 (41.8 ± 1.3 × 39.6 ± 1.25) and sporocysts 25.6–28.4 × 15.2–18.5 (27.1 ± 1.05 × 16.8 ± 1.22) from the chestnut Munia, Lonchura malacca (L.). The host birds belong to the order Passerorida.     

Eimeria clethrionomyis sp. n., Eimeria gallatii sp. n., Eimeria pileata sp. n. and Eimeria marconii sp. n. from the Red-Backed Vole Clethrionomys gapperi Vigors,from Pennsylvania§

SYNOPSIS Four new eimerian species are described from red-backed voles. Clethrionomys gapperi in Pennsylvania. Sporulated oocysts of Eimeria clethrionomyis sp. n. are ellipsoidal, 18.8 (16.5–21.5) × 14.9 (14.0–16.5) with elongate, ovoid sporocysts, 10.6 (9.5–12.0) × 6.1 (5.5–7.0). The oocyst wall is smooth, with 2 layers, and thins, with terminal cap at one or both ends. Polar granules, dark Stieda body and sporocyst residuum are present. The occyst residuum is absent. Sporulated oocysts of Eimeria gallatii sp. n. are ellipsoidal, 27.7 (21–32) × 19.3 (17–24) with ovoid sporocysts, 13.5 (12–15) × 8.8 (8–10). The oocyst wall is smooth, 2-layered, with a micropyle and thin wall at the end opposite the micropyle. Polar granules. Stieda body and sporocyst residuum are present. The oocyst residuum is atypical, of cobwebby material. Sporulated oocysts of Eimeria pileata sp. n. are subspherical to spherical, 25.2 (20.5–29.5) × 22.5(19.5–25.5) with ellipsoidal sporocysts, 13.4(10.5–15.0) × 8.4 (7.5–9.5). The oocyst wall is rough, pitted, striated, 2-layered, with no micropyle. Polar granules, oocyst and sporocyst residuum. Stieda body and stiedal cap are present. Sporulated oocysts of Eimeria marconii sp. n. are ellipsoidal, 13.0 (10.5–15.0) × 10.6 (9.5–12.0) with elongate, ovoid sporocysts, 7.7 (7.0–8.5) × 4.2 (3.0–4.5). The oocyst wall is smooth, single-layered, with no micropyle. Polar granules, dark Stieda body and sporocyst residuum are present. There is no oocyst residuum.     

Caryospora corallae n. sp. (Apicomplexa: Sporozoea, Eimeriidae) from the Emerald Tree Boa Corallus caninus (Serpentes: Boidae)

Oocysts of Caryospora corallae n. sp. were isolated from the feces of three Emerald Tree Boas Corallus caninus. The spherical oocysts of C. corallae averaged 22.4 μn (range 18.7 to 24.6) in diameter and were lacking a micropyle and oocyst residuum; a polar granule was present. The ovoid sporocysts measured 19.1(17.6-20.0) × 13.1(11.7-14.0) μm and a sporocyst residuum and a Stieda body were present. The oocyst wall was approximately 1 μm thick. The sporulation was completed in about 5–6 days at 23 ± 2°C. This is the first report of the genus Caryospora from Corallus caninus a member of the Boidae.     

Goussia girellae N. Sp. (Apicomplexa: Eimeriorina) in the Opaleye,Girella nigricans1

ABSTRACT Goussia girellae n. sp. is described from the opaleye fish, Girella nigricans. Merogonic stages were observed in the apices of intestinal epithelial cells, in the lamina propria, and in extra-intestinal sites including liver, gills, and spleen. Gamonts were observed in the intestinal epithelial cells. Only unsporulated oocysts were detected in the intestine, and sporulation occurred when feces containing oocysts were incubated for 48 h in seawater at 21°C. Oocysts are elongated (24.8 × 14.7 μm) with a wall about 200 nm thick and have no residuum, micropyle, or polar granule. Sporocysts are ellipsoid (8.5 × 4.5 μm), have a thin two-layered wall approximately 30 nm thick, and consist of two valves joined by a suture. Although moribund opaleye were also infected with Gyrodactylus sp., Cryptobia sp., Cardicola sp., and epitheliocystis organisms (chlamydia), all fish were heavily infected with G. girellae and morbidity was thus attributed to the coccidium.     

Aggregata polibraxiona n. sp. (Apicomplexa: Aggregatidae) from Octopus bimaculatus Verrill, 1883 (Mollusca: Cephalopoda) from the Gulf of California,Mexico

The Apicomplexa Aggregata spp. are intracellular parasites of cephalopods that infect the intestinal tract of commercially important species such as Octopus bimaculatus, which sustains the octopus fishery in Baja California (B.C.), Mexico. In this study, Aggregata polibraxiona n. sp. was described from the cecum of O. bimaculatus collected from Bahia de Los Angeles, B. C. Light and electron microscopy revealed that oocysts and sporocysts were spherical to ovoid in shape. Sporulated oocysts (293–835 × 177–688 μm) contained 135–674 sporocysts (12–24 × 11–22 μm). The sporocyst wall was covered by tubular projections (0.55–2.19 μm in length) bifurcated in the top, unevenly distributed, covered by a thin membrane. Each sporocyst contains 11–13 sporozoites (16–26 × 1.20–3 μm). Three partial sequences of the 18S rDNA gene were obtained, and two phylogenetic approaches were performed according to Bayesian inference and Maximum Likelihood. In both phylogenetic reconstructions, the sequences of A. polibraxiona n. sp. were recovered as a monophyletic group within the genus Aggregata and placed as a sister group to Aggregata octopiana Lineage II. Aggregata polibraxiona n. sp. is the first Apicomplexa described from a cephalopod host from Mexico and extends the geographical range of Apicomplexa infecting cephalopods.     

Eimeria melogale n. sp. (Apicomplexa: Eimeriidae) in the Javan ferret-badger (Melogale orientalis)

The Javan ferret-badger Melogale orientalis (Carnivora: Mustelidae: Helictidinae) is a small carnivore endemic to Indonesia. In the family Mustelidae, 10 Eimeria, 12 Cystoisopora, one Isospora, and one Hammondia species are known, but no eimeriid coccidia has been yet described in the subfamily Helictinidae (ferret badgers). Coproscopic examination of Javan ferret-badgers imported into the Czech Republic revealed the presence of coccidian oocysts. Sporulated oocysts differ from other Eimeria known in the family Mustelidae by their small size (12.4–16.1 × 10.4–13.4 μm) and ovoidal shape. Morphological data and phylogenetic analyses of 18S rRNA and COI genes indicated a new species of Eimeria found in faecal samples of Javan ferret badgers. The species is described as E. melogale n. sp.     

Eimeria diminuta sp. n. from the Ceylon Jungle Fowl,Callus lafayettei*

SYNOPSIS. Eimeria diminuta sp. n. is described from the Ceylon jungle fowl Gallus lafayettei. Its sporulated oocysts are 16.5 (16–17.5) × 14.5 (13.5 × 15.5) μm. The species produces patent infections in domestic fowl.     

Four New Species of Isospora from the Small Tree Finch (Camarhynchus parvulus) from the Galapagos Islands1

ABSTRACT. Four isosporan species are described from the small tree finch, Camarhynchus parvulus from Isabela Island on the Galapagos Archipelago. Isospora exigua n. sp. oocysts subspheroidal, one-layered, smooth, yellow-brown color, 20.4 × 20.1 (20-23 × 18-23) μm, with no micropyle, residuum, or polar body. Sporocysts ovoidal, 14 × 9.5 (13-15 × 8-10) μm, with small Stieda and substieda bodies and irregular-shaped residuum. Isospora rotunda n. sp. oocysts subspheroidal, single-layered, smooth, yellow-brown wall with large polar body and no micropyle or residuum, 20.9 × 20.8 (20-24 × 19-23) μm. Sporocysts ovoidal, 15 × 9.7 (13-16 × 9-10) μm with knob-like Stieda bodies, prominent substieda bodies and round residuum. Isospora fragmenta n. sp. oocysts subspheroidal with no micropyle or residuum but with many splinter-like polar granules and a smooth, colorless, single-layered wall, 25.3 × 24.2 (24-27 × 23-25) μm. Sporocysts piriform 15.4 × 11.5 (14-17 × 11-12) μm with knob-like Stieda bodies, prominent substieda bodies, and irregular-shaped residuum. Isospora temeraria n. sp. oocysts ellipsoidal with one polar body, no micropyle or residuum, and wall of a single layer, smooth, yellow-brown color, 25.4 × 21.1 (21-30 × 17-23) μm. Sporocysts piriform, 15 × 10 (14-15 × 9-11) μm with knob-like Stieda bodies, prominent substieda bodies, and a round residuum. One woodpecker finch, Cactospiza pallida, was found to be infected with I. exigua, and a warbler finch, Certhidea olivacea was infected with I. fragmenta.     

Eimeria malabaricas sp. n. and Eimeria bandipurensis from the South Indian Tree Squirrel,Funambulus tristriatus*

Synopsis. Oocysts of Eimeria malabaricas sp. n. and Eimeria bandipurensis from the South Indian tree squirrel Funambulus tristriatus collected in Kerala, India, are described. Sporulated oocysts of E. malabaricas were ellipsoid to subspherical measuring 39.8 (35–45) × 32.1 (29–37) m?m, with a thick (2.5–3.0 m?m), 2-layered wall. The outer layer was yellow-brown, striated, and rough. There was no micropyle. but a polar granule was present in 34% of oocysts. The sporocysts were ovoid, 16.0 (14.0–18.0) × 11.2 (11.0–12.0) m?m, with a Stieda body and a granular residuum. Excysted sporozoites were 21.8 (19.0–23.0) × 3.4 (3.0–4.0) m?m, with a large refractile body. The sporulated oocysts of E. bandipurensis are redescribed.     

Eimeria indianensis sp. n. and an Isospora sp. from the Opossum Didelphis virginiana (Kerr)*

Two of 15 road-killed opossums examined for coccidia were found to be infected with a hitherto undescribed species of Eimeria, herein named Eimeria indianensis . The oocysts were spherical (63%) or slightly subspherical (37%) with a double-layered wall. The outer layer was ～1.5 μm thick, yellowish, striated, and appeared rough and pitted on the surface. A micropyle was absent. The spherical oocysts were 16.3 (13–18) μm in diameter; the subspherical ones, 17.6 (15–18) × 16.4 (14–17) μm. The sporocysts measured 9.1 (8–10) × 6.2 (6–7) μm and contained a granular residuum. The sporozoites were elongate, measuring 13.4 (13–15) × 1.8 (1.6-2.0) μm; no refractile globules were seen. The prepatent period was 10 days and the patent period ranged from 9–15 days. A few oocysts of an Isospora sp. were present in one opossum. It was not possible to confirm whether they were specifically of the opossum or of spurious origin.