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1.
The aim of this work was to discover why pea (Pisum sativum L.) embryos recessive at the r locus (rr) have a higher lipid content than embryos dominant at this locus (RR). The r locus is a gene encoding starch-branching enzyme, rr embryos have a much lower activity of this enzyme than RR embryos, and hence a reduced rate of starch synthesis. The higher lipid content of rr embryos must be a consequence of this. We suggest that neither differences in the availability of substrate for lipid synthesis as a consequence of different rates of starch synthesis, nor differences in the capacity of the pathway for malonyl-CoA synthesis, account for the different lipid contents of RR and rr embryos. Lipid contents of the two sorts of embryo first diverge at a much later stage in development than divergence in starch content. Amounts of pyruvate and acetate, and activities of enzymes that convert triose phosphate to malonyl CoA are the same in the two sorts of embryo. Most of the lipid in developing embryos is polar, structural lipid, and polar lipid accounts for a large proportion of the difference in lipid content between the two sorts of embryo. This difference in structural-lipid content reflects considerable structural differences between the two sorts of embryo and is presumably the consequence of differences in rates of lipid turnover.Abbreviations DW
dry weight
- FW
fresh weight
- FAME
fatty-acid methyl esters
This work was supported by a grant-in-aid from the Agricultural and Food Research Council to the John Innes Institute. We are very grateful to Alan Jones for his valuable advice on lipid analysis and to Dr. Kay Denyer (Advanced Technologies, Cambridge, UK) for valuable discussions. We thank Dr. Cliff Hedley for the gift of the seed of the peas used in this work. 相似文献
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Evidence that the rb Locus Alters the Starch Content of Developing Pea Embryos through an Effect on ADP Glucose Pyrophosphorylase 总被引:4,自引:0,他引:4
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The aim of this work was to discover whether the rb locus of peas (Pisum sativum L.) affects seed starch content through action on an enzyme of starch synthesis in the developing embryo. The phenotypic effects of this locus are like those of the better characterised, unlinked r locus, which affects seed starch content through action on starch-branching enzyme. Embryos recessive at one or both of these loci (RRrbrb, rrRbRb, rrrbrb) have lower starch contents from an early stage of development than embryos dominant at these loci (RRRbRb). Maximum catalytic activities of enzymes of the pathway from sucrose to starch (sucrose synthase EC 2.4.1.13, UDP glucose pyrophosphorylase EC 2.7.7.9, ADP glucose pyrophosphorylase EC 2.7.7.27, ADP glucose-starch synthase EC 2.4.1.21, starch-branching enzyme EC 2.4.1.18) were compared in developing embryos of three lines of rbrb peas and four lines of RbRb peas. The only consistent difference between the two sorts of embryo was in the activity of ADP glucose pyrophosphorylase, which was at least tenfold lower in rbrb than in RbRb embryos. The activity in rbrb embryos was in most cases less than the estimated rate of starch synthesis of RRRbRb embryos. We conclude that the effect of the rb locus on the starch content of pea seeds is mediated through an alteration in the activity of ADP glucose pyrophosphorylase in the developing embryo. 相似文献
5.
Starch branching enzymes belonging to distinct enzyme families are differentially expressed during pea embryo development 总被引:23,自引:0,他引:23
Rachel A. Burton J. Derek Bewley Alison M. Smith Madan K. Bhattacharyya Helma Tatge Steve Ring Vicky Bull William D.O. Hamilton Cathie Martin 《The Plant journal : for cell and molecular biology》1995,7(1):3-15
cDNA clones for two isoforms of starch branching enzyme (SBEI and SBEII) have been isolated from pea embryos and sequenced. The deduced amino acid sequences of pea SBEI and SBEII are closely related to starch branching enzymes of maize, rice, potato and cassava and a number of glycogen branching enzymes from yeast, mammals and several prokaryotic species. In comparison with SBEI, the deduced amino acid sequence of SBEII lacks a flexible domain at the N-terminus of the mature protein. This domain is also present in maize SBEII and rice SBEIII and resembles one previously reported for pea granule-bound starch synthase II (GBSSII). However, in each case it is missing from the other isoform of SBE from the same species. On the basis of this structural feature (which exists in some isoforms from both monocots and dicots) and other differences in sequence, SBEs from plants may be divided into two distinct enzyme families. There is strong evidence from our own and other work that the amylopectin products of the enzymes from these two families are qualitatively different. Pea SBEI and SBEII are differentially expressed during embryo development. SBEI is relatively highly expressed in young embryos whilst maximum expression of SBEII occurs in older embryos. The differential expression of isoforms which have distinct catalytic properties means that the contribution of each SBE isoform to starch biosynthesis changes during embryo development. Qualitative measurement of amylopectin from developing and maturing embryos confirms that the nature of amylopectin changes during pea embryo development and that this correlates with the differential expression of SBE isoforms. 相似文献
6.
Mutations at the
rug4
locus alter the carbon and nitrogen metabolism of pea plants through an effect on sucrose synthase 总被引:5,自引:2,他引:3
Josephine Craig Paul Barratt Helma Tatge Annabelle Déjardin Linda Handley Christopher D. Gardner Lorraine Barber Trevor Wang Cliff Hedley Cathie Martin Alison M. Smith 《The Plant journal : for cell and molecular biology》1999,17(4):353-362
The biochemical and molecular basis of the wrinkled-seeded phenotype of rug4 mutants of pea ( Pisum sativum L.) has been investigated. Mutant embryos have reduced starch contents and only 5% of the sucrose synthase activity of wild-type embryos during development. Activities of other enzymes involved in the conversion of sucrose to starch are unaffected. A gene encoding an isoform of sucrose synthase expressed in the embryo co-segregates with the rug4 locus, and one of the three mutant alleles has been show to carry a point mutation in this gene that converts a highly conserved arginine residue to a lysine residue. It is highly likely that the reduced starch content of the mutant embryo is a direct consequence of the loss of sucrose synthase activity. The mutations reduce the activity of sucrose synthase in the testa and the leaf by 50% or less, but activity in Rhizobium -infected root nodules is reduced by 85%. Although the nodules of mutant plants contain metabolically active bacteroids, the N content and δ15N values of these plants in the field indicate that, unlike wild-type plants, they derive little of their N from N2 fixation via Rhizobium . Sucrose synthase thus appears to be essential for the supply of carbon for bacteroid metabolism and/or ammonia assimilation during nitrogen assimilation. 相似文献
7.
The rb Mutation of Peas Causes Structural and Regulatory Changes in ADP Glucose Pyrophosphorylase from Developing Embryos 总被引:7,自引:3,他引:4
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A mutation at the rb locus of pea (Pisum sativum L.) alters the shape, reduces the starch content, and increases the lipid and sucrose contents of the seed. These effects are probably all consequences of a reduction of up to 40-fold in the maximum catalytic activity of ADP glucose pyrophosphorylase in the developing embryo of the mutant relative to the wild type. We have investigated how the mutation brings about this reduction in activity. The purified enzyme from mutant embryos has a specific activity about 10-fold lower than that from wild-type embryos, and it is much more sensitive to the effectors inorganic phosphate and 3-phosphoglycerate than the wild-type enzyme. Both wild-type and mutant enzymes consist of polypeptides of around 50 kilodaltons. One of the polypeptides of the purified wild-type enzyme is missing from the mutant enzyme. We deduce that in the wild-type embryo this protein may interact with other subunits to confer a high specific activity and a low susceptibility to effectors on the enzyme. 相似文献
8.
WANG T. L.; COOK S. K.; FRANCIS R. J.; AMBROSE M. J.; HEDLEY C. L. 《Journal of experimental botany》1987,38(11):1921-1932
The abscisic acid (ABA) content of wrinkled (rr) pea seed tissueshas been quantified during development using multiple-ion-monitoringcombined gas chromatography-mass spectrometry and a deuteratedinternal standard. The level of ABA in the embryo generallyincreased with increasing cotyledon fresh weight while thatin the testa showed a distinct maximum at the time of maximumendosperm volume and the slowing in the growth of the testa.Pods contained relatively little ABA on a fresh weight basis.The total seed ABA content showed a biphasic distribution, thefirst maximum following the maximum growth rate of the testaand the second that of the embryo. The biphasic distributionof ABA in the pea seed was confirmed using a second pea genotype,near-isogenic to the first except for the r locus, and by theanalysis of individual seeds using a radioimmunoassay for ABA.The first maximum was composed mainly of a testa component andthe second mainly of an embryo component. When plants were grownin different environments, wrinkled seeds were found to containslightly more ABA than round (RR) but this was only significantlate in development. Immature seeds were capable of metabolizing17'-deoxy ABA to ABA, as determined by incorporation of either3H or 2H, and the metabolite was present mainly in the testa.The production of ABA in pea seeds is discussed in relationto the development of the different seed tissues. Key words: Abscisic acid, peas, seed development 相似文献
9.
Isoforms of starch synthase (EC 2.4.1.21) in pea (Pisum sativum L.) leaves have been identified and compared with those in developing pea embryos. Purification and immunoprecipitation experiments
show that most of the soluble starch synthase activity of the leaf is contributed by a novel isoform (SSIII) that is antigenically
related to the major soluble isoform of the potato tuber. The major soluble isoform of the embryo (SSII) is also present in
the leaf, but contributes only 15% of the soluble activity. Study of the leaf starch of lam mutant peas, which lack the abundant granule-bound isoform responsible for amylose synthesis in the embryo (GBSSI), indicates
that GBSSI is not responsible for the synthesis of amylose-like material in the leaf. Leaves appear to contain a novel granule-bound
isoform, antigenically related to GBSSI. The implications of the results for understanding of the role of isoforms of starch
synthase are discussed.
Received: 13 March 1997 / Accepted: 13 May 1997 相似文献
10.
A mutation at the rb gene, lowering ADPGPPase activity, affects storage product metabolism of pea seed coats 总被引:1,自引:0,他引:1
Rochat Christine; Wuillme Sylvie; Boutin Jean-Pierre; Hedley Cliff L. 《Journal of experimental botany》1995,46(4):415-421
Seed coat development was studied on two nearisogenic linesof peas (Pisum sativum L.): RbRb (wild type, round seed) andrbrb (wrinkled seed). A mutation at the rb locus modifies thedry seed shape and reduces the starch content of the embryo.This mutation is now known to affect the activity of ADPGlucosepyrophosphorylase, a key enzyme in the starch biosynthetic pathway.We have investigated the effects of the rb mutation on seedcoat development and found that the mutation reduces the growthrate and starch content in this organ. However, experimentson the kinetics of 14C-sucrose loading showed that starch synthesisfrom unloaded sucrose occurred in the seed coat for both mutantand wild-type lines. In addition, the sucrose concentrationwas increased and amino acid concentration decreased such thatthe nutritional balance of the embryos was affected. However,osmolality of the seed coat cells was not affected, suggestinga regulatory process which allows the maintenance of the importof assimilates in the seeds of either line. Key words: ADPGlucose pyrophosphorylase, seed coat, seed development, starch metabolism, wrinkled seed 相似文献
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In maize, three isoforms of starch-branching enzyme, SBEI, SBEIIa, and SBEIIb, are encoded by the Sbe1a, Sbe2a, and Amylose extender (Ae) genes, respectively. The objective of this research was to explore the effects of null mutations in the Sbe1a and Ae genes alone and in combination in wx background on kernel characteristics and on the morphology and physical behavior of endosperm starch granules. Differences in kernel morphology and weight, starch accumulation, starch granule size and size distribution, starch microstructure, and thermal properties were observed between the ae wx and sbe1a ae wx plants but not between the sbe1a wx mutants when compared to wx. Starch from sbe1a ae wx plants exhibited a larger granule size with a wider gelatinization temperature range and a lower endotherm enthalpy than ae wx. Microscopy shows weaker iodine staining in sbe1a ae wx starch granules. X-ray diffraction revealed A-type crystallinity in wx and sbe1a wx starches and B-type in sbe1a ae wx and ae wx. This study suggests that, while the SBEIIb isoform plays a dominant role in maize endosperm starch synthesis, SBEI also plays a role, which is only observable in the presence of the ae mutation. 相似文献
15.
Changes in carbohydrate metabolism and assimilate export in starch-excess mutants of Arabidopsis 总被引:6,自引:0,他引:6
The aim of this work was to investigate the effects on carbohydrate metabolism of a reduction in the capacity to degrade leaf starch in Arabidopsis. The major roles of leaf starch are to provide carbon for sucrose synthesis, respiration and, in developing leaves, for biosynthesis and growth. Wild-type plants were compared with plants of a starch-excess mutant line (sex4) deficient in a chloroplastic isoform of endoamylase. This mutant has a reduced capacity for starch degradation, leading to an imbalance between starch synthesis and degradation and the gradual accretion of starch as the leaves age. During the night the conversion of starch into sucrose in the mutant is impaired; the leaves of the mutant contained less sucrose than those of the wild type and there was less movement of 14C-label from starch to sucrose in radio-labelling experiments. Furthermore, the rate of assimilate export to the roots during the night was reduced in the mutant compared with the wild type. During the day however, photosynthetic partitioning was altered in the mutant, with less photosynthate partitioned into starch and more into sugars. Although the sucrose content of the leaves of the mutant was similar to the wild type during the day, the rate of export of sucrose to the roots was increased more than two-fold. The changes in carbohydrate metabolism in the mutant leaves during the day compensate partly for its reduced capacity to synthesize sucrose from starch during the night. 相似文献
16.
Contribution of sucrose synthase, ADP-glucose pyrophosphorylase and starch synthase to starch synthesis in developing pea seeds 总被引:11,自引:0,他引:11
Using genetic variability existing amongst nine pea genotypes (Pisum sativum L.), the biochemical basis of sink strength in developing pea seeds was investigated. Sink strength was considered to be reflected by the rate of starch synthesis (RSS) in the embryo, and sink activity in the seed was reflected by the relative rate of starch synthesis (RRSS). These rates were compared to the activities of three enzymes of the starch biosynthetic pathway [sucrose synthase (Sus), ADP-glucose pyrophosphorylase and starch synthase] at three developmental stages during seed filling (25, 50 and 75% of the dry seed weight). Complete sets of data collected during seed filling for the nine genotypes showed that, for all enzyme activities (expressed on a protein basis), only Sus in the embryo and seed coat was linearly and significantly correlated to RRSS. The contribution of the three enzyme activities to the variability in RSS and RRSS was evaluated by multiple regression analysis for the first two developmental stages. Only Sus activity in the embryo could explain, at least in part, the significant variability observed for both the RSS and the RRSS at each developmental stage. We conclude that Sus activity is a reliable marker of sink activity in developing pea seeds. 相似文献
17.
Isolation of a cDNA encoding a granule-bound 152-kilodalton starch-branching enzyme in wheat 总被引:5,自引:0,他引:5
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Screening of a wheat (Triticum aestivum) cDNA library for starch-branching enzyme I (SBEI) genes combined with 5'-rapid amplification of cDNA ends resulted in isolation of a 4,563-bp composite cDNA, Sbe1c. Based on sequence alignment to characterized SBEI cDNA clones isolated from plants, the SBEIc predicted from the cDNA sequence was produced with a transit peptide directing the polypeptide into plastids. Furthermore, the predicted mature form of SBEIc was much larger (152 kD) than previously characterized plant SBEI (80-100 kD) and contained a partial duplication of SBEI sequences. The first SBEI domain showed high amino acid similarity to a 74-kD wheat SBEI-like protein that is inactive as a branching enzyme when expressed in Escherichia coli. The second SBEI domain on SBEIc was identical in sequence to a functional 87-kD SBEI produced in the wheat endosperm. Immunoblot analysis of proteins produced in developing wheat kernels demonstrated that the 152-kD SBEIc was, in contrast to the 87- to 88-kD SBEI, preferentially associated with the starch granules. Proteins similar in size and recognized by wheat SBEI antibodies were also present in Triticum monococcum, Triticum tauschii, and Triticum turgidum subsp. durum. 相似文献
18.
An Analysis of Seed Development in Pisum sativum II. The Effect of the r-Locus on the Growth and Development of the Seed 总被引:1,自引:0,他引:1
The r-locus is one of the few genetic loci known to affect thestorage product composition and the morphology of pea (Pisumsativum) seeds. Lines which are near-isogenic except for ther-locus have been developed to study the effects of this locuson seed development. The plant phenotypes of these lines werevery similar except for those characteristics previously attributedto the r-locus. The seed development of the two lines followedsimilar patterns until the endosperm was absorbed by the embryo.The fresh weight of the rr line then increased more rapidlydue to the overall effect of a higher rate of water uptake anda lower rate of dry weight increase of the rr embryos comparedwith embryos homozygous for the R-allele. The effect of the r-locus on the relationship between embryofresh weight and dry weight suggests that the alleles may beaffecting the osmotic regulation of the developing embryo. Pisum sativum, pea, seed development, r-locus, genetic variation, growth analysis 相似文献
19.
Alison M. Smith 《Planta》1988,175(2):270-279
In order to determine whether round-and wrinkled-seeded peas (Pisum sativum L.) differ in the activity and properties of starch-branching enzyme (1,4--D-glucan, 1,4--D-glucan-6-glycosyl transferase; EC 2.4.1.18) in their developing embryos, essentially isogenic lines of peas, differing only at the r (rugosus) locus that confers the round (RR, Rr) or wrinkled (rr) phenotype, were studied. Activity of the enzyme rises rapidly from an early stage of development in embryos of round peas, but only at later stages in embryos of wrinkled peas. The purified enzyme from mature embryos of round peas can be resolved into two isoforms that differ in molecular weight and in their ability to branch amylose. The purified enzyme from mature embryos of wrinkled peas is a single protein with the same molecular weight and branching properties as one of the isoforms from embryos of round peas. The difference in activity of starch-branching enzyme between embryos of round and wrinkled peas is likely to be due to the absence from embryos of wrinkled peas of one of the isoforms occurring in embryos of round peas.Abbreviations DEAE
diethylaminoethyl
- FW
fresh weight
- kDa
kilodalton
- SDS
sodium dodecyl sulfate 相似文献
20.
Mutations that reduced the rate of starch synthesis in pea (Pisum sativum L.) embryos through effects on enzymes on the pathway from sucrose to adenosine 5′-diphosphoglucose (ADPglucose) also led
to a reduction in the amylose content of the starch of developing embryos. Evidence is presented that this relationship between
rate of synthesis and the composition of starch is due to the fact that amylopectin-synthesising isoforms of starch synthase
have higher affinities for ADPglucose than the amylose-synthesising isoform. First, developing mutant embryos (rb, rug3 and rug4 mutants) displayed both reduced amylose contents in their starches and reduced ADPglucose contents relative to wild-type
embryos. Second, incubation of detached, wild-type embryos for 6 h at high and low glucose concentrations resulted in differences
in both ADPglucose content and the relative rates of amylose and amylopectin synthesis. At 0.25 M glucose both ADPglucose
content and the proportion of synthesised starch that was amylose were about twice as great as at 25 μM glucose. Third, S
0.5 values for soluble (amylopectin-synthesising) starch synthases in developing embryos were several-fold lower than that for
granule-bound (amylose synthesising) starch synthase. Estimates of the expected amylose contents of the starch of the mutant
embryos, based on the reduction in their ADPglucose contents and on the S
0.5 values of the starch synthases, were very similar to the measured amylose contents. The implications of these results for
the determination of starch composition are discussed.
Received: 6 February 1999 / Accepted: 22 May 1999 相似文献