共查询到20条相似文献,搜索用时 31 毫秒
1.
A. J. Worland V. Korzun M. S. Röder M. W. Ganal C. N. Law 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(8):1110-1120
Wheat microsatellite WMS 261 whose 192-bp allele has been shown to be diagnostic for the commercially important dwarfing
gene Rht8 was used to screen over 100 wheat varieties to determine the worldwide spread of Rht8. The results showed Rht8 to be widespread in southern European wheats and to be present in many central European wheats including the Russian varieties
‘Avrora’, ‘Bezostaya’ and ‘Kavkaz’. Rht8 appears to be of importance to South European wheats as alternative giberellic acid (GA)-insensitive dwarfing genes do not
appear to be adapted to this environment. The very successful semi-dwarf varieties bred by CIMMYT, Mexico, for distribution
worldwide have been thought to carry Rht8 combined with GA-insensitive dwarfing genes. Additional height reduction would have been obtained from pleiotropic effects
of the photoperiod-response gene Ppd1 that is essential to the adaptability of varieties bred for growing under short-winter days in tropical and sub-tropical
areas. The microsatellite analysis showed that CIMMYT wheats lack Rht8 and carry a WMS 261 allelic variant of 165 bp that has been associated with promoting height. This presumably has adaptive
significance in partly counteracting the effects of other dwarfing genes and preventing the plants being too short. Most UK,
German and French wheats carry an allelic variant at the WMS 261 locus with 174 bp. This could be selected because of linkage
with the recessive photoperiod-sensitive ppd1 allele that is thought to offer adaptive significance northern European wheats.
Received: 17 October 1997 / Accepted: 12 November 1997 相似文献
2.
The use of codominant microsatellite molecular markers allows one to study the inheritance and distribution of alleles linked to important agronomic characters. A microsatellite locus WMS261 tightly linked to a dwarfing gene Rht8 was analyzed in wheat cultivars and selection material of the Institute of Plant Breeding and Genetics. PCR screening of common wheat cultivars produced in the southern Ukraine showed the prevalence of a 192-bp allele at locus WMS261 that indicates adaptive significance of a corresponding allele of the Rht8 gene in the southern regions. 相似文献
3.
Allele Distribution at Locus WMS261Marking the Dwarfing Gene Rht8in Common Wheat Cultivars of Southern Ukraine 总被引:1,自引:0,他引:1
The use of codominant microsatellite molecular markers allows one to study the inheritance and distribution of alleles linked to important agronomic characters. A microsatellite locus WMS261tightly linked to a dwarfing geneRht8was analyzed in wheat cultivars and selection material of the Institute of Plant Breeding and Genetics. PCR screening of common wheat cultivars produced in the southern Ukraine showed the prevalence of a 192-bp allele at locus WMS261that indicates adaptive significance of a corresponding allele of the Rht8gene in the southern regions. 相似文献
4.
J.-H. Lee Y. Yen K. Arumuganathan P. S. Baenziger 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1300-1304
Two complete, independently maintained sets of 21 monosomic wheat lines derived from cv. ‘Chinese Spring’ were analyzed for
their DNA content at the G1 stage with flow cytometry. The DNA content of individual chromosomes was estimated by subtracting
the value of a monosomic line from that of euploid wheat. Our data show that the estimated 2C DNA of individual wheat chromosomes
in 21 monosomics at the G1 stage ranges from about 0.58 pg in chromosome 1D to approximately 1.12 pg in chromosome 3A. The
A genome (2C=6.15 pg) seems to contain more DNA than the B (2C=6.09 pg) and D (2C=5.05 pg) genomes. Analysis of variance showed
significant differences (α=0.01) in DNA content both among homoeologous groups and among genomes. Our estimates of interphase
DNA content of wheat chromosomes from monosomic lines were poorly correlated to the chromosome sizes at metaphase (r=0.622, P≤0.01). This poor correlation might be due to differential coiling among chromosomes during cell division, possible bias of
fluorochrome binding to heterochromatin, or heterogeneity among monosomic lines. Finally, flow cytometry may aid but cannot
replace cytological checks in aneuploid maintenance.
Received: 21 January 1997 / Accepted: 23 June 1997 相似文献
5.
Resistance to frost, winterhardiness, yield and its components were investigated in recombinant-inbred lines F5 Odesskaya 16/Bezostaya 1 and 61 winter wheat varieties differing on the alleles of Rht8 gene. The absence of influence of gene Rht8 different alleles on the tested traits was shown. The high frequency of distribution of alleles Rht8c in modern wheat varieties developed in Plant Breeding and Genetics Institute is a result of high frequency of use of this allele in genetic pool after 1959. 相似文献
6.
H. Buerstmayr M. Lemmens G. Fedak P. Ruckenbauer Communicated B. S. Gill 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(1):76-85
Fusarium head blight (FHB or scab) caused by Fusarium spp. is a widespread disease of cereals causing yield and quality losses and contaminating cereal products with mycotoxins. The
breeding of resistant varieties is the method of choice for controlling the disease. Unfortunately, the genetic basis of scab
resistance is still poorly understood. We present the results of a back-cross reciprocal monosomic analysis of FHB resistance
using the highly resistant Hungarian winter wheat line ‘U-136.1’ and the highly susceptible cultivar ‘Hobbit-sib’. Resistance
testing was performed in a field trial artificially inoculated with a Fusarium culmorum conidial suspension. Five hemizygous families containing ‘U-136.1’ chromosomes 6B, 5A, 6D, 1B, and 4B had a visually reduced
spread of infection compared to lines having the ‘Hobbit-sib’ chromosome. Chromosome 2B from ‘U-136.1’ had an increased spread
of infection. The critical chromosomes controlling seed weight were 6D, 3B, 5A, and 6B while those controlling deoxynivalenol
(DON) content were homoeologous groups 2 and 6, although the latter effects were not significant due to a high coefficient
of variation. Results from this and other studies show that chromosomes 6D, 6B, 5A, 4D, and 7A have frequently been associated
with scab resistance in a number of wheat cultivars. Research groups now attempting to map scab resistance in wheat using
markers should pay special attention to the above-mentioned chromosomes.
Received: 31 March 1998 / Accepted: 14 July 1998 相似文献
7.
Isabelle Bertin Lesley Fish Tracie N. Foote Emilie Knight John Snape Graham Moore 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(8):1371-1381
Breeders can force sexual hybridisation between wheat and related grass species to produce interspecific hybrids containing
a dihaploid set of wheat and related chromosomes. This facilitates the introgression of desirable genes into wheat from the
secondary gene pool. However, most elite European wheat varieties carry genes that suppress crossability, making the transfer
of novel traits from exotic germplasm into elite wheat varieties difficult or impossible. Previous studies have identified
at least five crossability loci in wheat. Here, the crossability locus with the largest effect, Kr1 on chromosome arm 5BL, was fine-mapped by developing a series of recombinant substitution lines in which the genome of the
normally non-crossable wheat variety ‘Hobbit sib’ carries a recombinant 5BL chromosome arm containing segments from the crossable
variety ‘Chinese Spring’. These recombinant lines were scored for their ability to cross with rye over four seasons. Analysis
revealed at least two regions on 5BL affecting crossability, including the Kr1 locus. However, the ability to set seed is highly dependent on prevailing environmental conditions. Typically, even crossable
wheat lines exhibit little or no seed set when crossed with rye in winter, but show up to 90% seed set from similar crosses
made in summer. By recombining different combinations of the two regions affecting crossability, wheat lines that consistently
exhibit up to 50% seed set, whether crossed in the UK winter or summer conditions, were generated, thus creating a very important
tool for increasing the efficiency of alien wheat transfer programmes. 相似文献
8.
H. Zhang J. Jia M. D. Gale K. M. Devos 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):69-75
A comparative genetic map of Aegilops umbellulata with wheat was constructed using RFLP probes that detect homoeoloci previously mapped in hexaploid bread wheat. All seven
Ae. umbellulata chromosomes display one or more rearrangements relative to wheat. These structural changes are consistent with the sub-terminal
morphology of chromosomes 2 U, 3 U, 6 U and 7 U. Comparison of the chromosomal locations assigned by mapping and those obtained
by hybridization to wheat/Ae. umbellulata single chromosome addition lines verified the composition of the added Ae. umbellulata chromosomes and indicated that no further cytological rearrangements had taken place during the production of the alien-wheat
aneuploid lines. Relationships between Ae. umbellulata and wheat chromosomes were confirmed, based on homoeology of the centromeric regions, for 1 U, 2 U, 3 U, 5 U and 7 U. However,
homoeology of the centromeric regions of 4 U with wheat group-6 chromosomes and of 6 U with wheat group-4 chromosomes was
also confirmed, suggesting that a re-naming of these chromosomes may be pertinent. The consequences of the rearrangements
of the Ae. umbellulata genome relative to wheat for gene introgression are discussed.
Received: 10 July 1997 / Accepted: 19 September 1997 相似文献
9.
T. Burnouf J. A. Bietz 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1985,70(6):610-619
Summary Glutenin subunits from nullisomic-tetrasomic and ditelocentric lines of the hexaploid wheat variety ‘Chinese Spring’ (CS)
and from substitution lines of the durum wheat variety ‘Langdon’ were fractionated by reversed-phase high-performance liquid
chromatography (RP-HPLC) at 70 °C using a gradient of acetonitrile in the presence of 0.1% trifluoroacetic acid. Nineteen
subunits were detected in CS. The presence and amounts of four early-eluted subunits were found, through aneuploid analysis,
to be controlled by the long arms of chromosomes 1D (1DL) (peaks 1–2) and 1B (1BL) (peaks 3–4). Sodium dodecyl sulfate-polyacrylamide
gel electrophoresis showed that these four subunits are the high molecular weight subunits of glutenin, which elute in the
order 1Dy, 1Dx, 1By, and 1Bx. Similar amounts of 1DL subunits were present (6.3 and 8.8% of total glutenin), but 1BL subunits
differed more in abundance (5.4 and 9.5%, respectively). Results indicate that most late-eluting CS glutenin subunits were
coded by structural genes on the short arms of homoeologous group 1 chromosomes: 6 by 1DS, 5 by 1AS, and 4 by 1BS. Glutenin
of tetraploid ‘Langdon’ durum wheat separated into nine major subunits: 6 were coded by genes on 1B chromosomes, and 3 on
1A chromosomes. Gene locations for glutenin subunits in the tetraploid durum varieties ‘Edmore’ and ‘Kharkovskaya-5’ are also
given. These results should make RP-HPLC a powerful tool for qualitative and quantitative genetic studies of wheat glutenin.
The mention of firm names or trade products does not imply that they are endorsed or recommended by the U.S. Department of
Agriculture over other firms or similar products not mentioned
Stationed at the Northern Regional Research Center, Peoria. 相似文献
10.
Common loci underlie field resistance to soybean sudden death syndrome in Forrest,Pyramid, Essex,and Douglas 总被引:8,自引:0,他引:8
Njiti VN Meksem K Iqbal MJ Johnson JE Kassem MA Zobrist KF Kilo VY Lightfoot DA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(2-3):294-300
Soybean [Glycine max (L.) Merr.] sudden death syndrome (SDS) caused by Fusarium solani f. sp. glycines results in severe yield losses. Resistant cultivars offer the most-effective protection against yield losses but resistant
cultivars such as ’Forrest’ and ’Pyramid’ vary in the nature of their response to SDS. Loci underlying SDS resistance in ’Essex’ × Forrest
are well defined. Our objectives were to identify and characterize loci and alleles that underlie field resistance to SDS
in Pyramid×’Douglas’. SDS disease incidence and disease severity were determined in replicated field trials in six environments
over 4 years. One hundred and twelve polymorphic DNA markers were compared with SDS disease response among 90 recombinant
inbred lines from the cross Pyramid×Douglas. Two quantitative trait loci (QTLs) for resistance to SDS derived their beneficial
alleles from Pyramid, identified on linkage group G by BARC-Satt163 (261-bp allele, P=0.0005, R2=16.0%) and linkage group N by BARC-Satt080 (230-bp allele, P=0.0009, R2=15.6%). Beneficial alleles of both QTLs were previously identified in Forrest. A QTL for re- sistance to SDS on linkage group
C2 identified by BARC-Satt307 (292-bp allele, P=0.0008, R2=13.6%) derived the beneficial allele from Douglas. A beneficial allele of this QTL was previously identified in Essex. Recombinant
inbred lines that carry the beneficial alleles for all three QTLs for resistance to SDS were significantly (P≤0.05) more resistant than other recombinant inbred lines . Among these recombinant inbred lines resistance to SDS was environmentally
stable. Therefore, gene pyramiding will be an effective method for developing cultivars with stable resistance to SDS.
Received: 20 October 1999 / Accepted: 22 May 2001 相似文献
11.
Kuchel H Williams KJ Langridge P Eagles HA Jefferies SP 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(8):1029-1041
Grain yield forms one of the key economic drivers behind a successful wheat (Triticum aestivum L.) cropping enterprise and is consequently a major target for wheat breeding programmes. However, due to its complex nature,
little is known regarding the genetic control of grain yield. A doubled-haploid population, comprising 182 individuals, produced
from a cross between two cultivars ‘Trident’ and ‘Molineux’, was used to construct a linkage map based largely on microsatellite
molecular makers. ‘Trident’ represents a lineage of wheat varieties from southern Australia that has achieved consistently
high relative grain yield across a range of environments. In comparison, ‘Molineux’ would be rated as a variety with low to
moderate grain yield. The doubled-haploid population was grown from 2002 to 2005 in replicated field experiments at a range
of environments across the southern Australian wheat belt. In total, grain yield data were recorded for the population at
18 site-year combinations. Grain yield components were also measured at three of these environments. Many loci previously
found to be involved in the control of plant height, rust resistance and ear-emergence were found to influence grain yield
and grain yield components in this population. An additional nine QTL, apparently unrelated to these traits, were also associated
with grain yield. A QTL associated with grain yield on chromosome 1B, with no significant relationship with plant height,
ear-emergence or rust resistance, was detected (LOD ≥2) at eight of the 18 environments. The mean yield, across 18 environments,
of individuals carrying the ‘Molineux’ allele at the 1B locus was 4.8% higher than the mean grain yield of those lines carrying
the ‘Trident’ allele at this locus. Another QTL identified on chromosome 4D was also associated with overall gain yield at
six of the 18 environments. Of the nine grain yield QTL not shown to be associated with plant height, phenology or rust resistance,
two were located near QTL associated with grain yield components. A third QTL, associated with grain yield components at each
of the environments used for testing, was located on chromosome 7D. However, this QTL was not associated with grain yield
at any of the environments. The implications of these findings on marker-assisted selection for grain yield are discussed. 相似文献
12.
G. D. Parker K. J. Chalmers A. J. Rathjen P. Langridge 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):238-245
An RFLP map constructed using 150 single seed descent (SSD) lines from a cross between two hexaploid wheat varieties (‘Schomburgk’בYarralinka’)
was used to identify loci controlling flour colour. Flour colour data were obtained from field trials conducted over two seasons
at different sites. The estimated heritability of this trait was calculated as 0.67. Two regions identified in the preliminary
analysis on chromosomes 3A and 7A, accounted for 13% and 60% of the genetic variation respectively. A detailed analysis of
the major locus on 7A was conducted through fine mapping of AFLP markers identified using bulked segregant analysis (BSA).
Seven additional markers were identified by the BSA and mapped to the region of the 7A locus. The applicability of these markers
to identify wheat lines with enhanced flour colour is discussed.
Received: 30 September 1997 / Accepted: 4 February 1998 相似文献
13.
V. Ivandic S. Malyshev V. Korzun A. Graner A. Börner 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(5):728-731
We report the genetic mapping of Dwf2, a dominant gibberellic acid (GA3)-insensitive dwarfing gene which has been previously described to cause a very short growth habit in barley (Hordeum vulgare) mutant ‘93/B694’. Using RFLP and microsatellite markers we performed segregation analysis in an F2 population comprising 86 individuals developed from a cross of ‘93/B694’ (Dwf2) with ‘Bonus M2’ (dwf2). Dwf2 was mapped on the short arm of barley chromosome 4H proximal to microsatellite marker XhvOle (5.7 cM) and distal to RFLP marker Xmwg2299 (18.3 cM). The genetic localization of the Dwf2 gene at a homoeologous position to the multiallelic Rht-B1 and Rht-D1 loci in wheat suggests synteny of GA-insensitive dwarfing genes within the Triticeae. Moreover, the extremely prostrate growth habit exhibited in barley ‘93/B694’ (Dwf2) resembles that of wheat plants carrying the genes Rht-B1c (Rht3) or Rht-D1c (Rht10).
Received: 1 July 1998 / Accepted: 17 September 1998 相似文献
14.
J. Wang K. D. Liu C. G. Xu X. H. Li Q. Zhang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(3):407-412
Wide-compatibility varieties (WCVs) are a special class of rice germplasm that is able to produce fertile hybrids when crossed
to both indica and japonica rice varieties. WCVs may differ greatly in their spectrum and level of compatibility. The objective
of this study was to determine the genetic basis of wide-compatibility conferred by ‘Dular’, a landrace variety from India
that has demonstrated a high level of wide-compatibility in previous studies with a broad range of indica and japonica varieties.
A three-way cross (‘Balilla/Dular//Nanjing 11’) was made and the resulting F1 population evaluated in the field for spikelet fertility. A total of 235 plants from this population was assayed individually
for restriction fragment length polymorphisms (RFLPs) at 159 marker loci covering the entire rice genome at regular intervals.
Quantitative trait locus (QTL) analysis identified 5 loci, located on chromosomes 1, 3, 5, 6 and 8, as having significant
effects on hybrid fertility, which jointly explained 55.5% of the fertility variation in this population. The QTL on chromosome
5 ( f5) showed the largest effect on hybrid fertility, followed by those on chromosomes 6 ( f6), 3 ( f3) and 1 ( f1), with the one on chromosome 8 ( f8) having the smallest effect. Genotypes each composed of an allele from ‘Dular’ and an allele from ‘Nanjing 11’ at four ( f3, f5, f6 and f8) of the five QTLs contributed to the increase of fertility in the population. In contrast, the genotype composed of alleles
from ‘Balilla’ and ‘Nanjing 11’ at the fifth locus ( f1) was in the direction of increasing fertility. Analysis of variance using marker genotypes at the five QTLs as the groups
detected two interactions involving four of the five loci, a 2-locus interaction between f5 and f8 and a 3-locus interaction among f3, f5 and f6. The level of hybrid fertility is the result of complex interactions among these loci. The implication of the present findings
in the utilization of the wide-compatibility of ‘Dular’ in rice breeding programs is also discussed.
Received: 21 October 1997 / Accepted: 30 December 1997 相似文献
15.
In the south part of Ukraine the haplotype of Rht8c and Ppd-D1 genes is widely distributed among modern bread wheat varieties. During the time of scientific breeding program it has been selected as one of the most important adaptive complexes for plants of this region. The genetic distance between the Rht8 and Ppd-D1 genes was clarified. 相似文献
16.
Sardesai N Nemacheck JA Subramanyam S Williams CE 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(6):1167-1173
H32 is a newly identified gene that confers resistance to the highly pervasive Biotype L of the Hessian fly [ Mayetiola destructor (Say)]. The gene was identified in a synthetic amphihexaploid wheat, W-7984, that was constructed from the durum ‘Altar 84’ and Aegilops tauschii. This synthetic wheat is one of the parents of the marker-rich ITMI population, which consists of 150 recombinant inbred lines (RILs) derived by single-seed descent from a cross with ‘Opata 85’. Linkage analysis of the H32 locus in the ITMI population placed the gene between flanking microsatellite (SSR) markers, Xgwm3 and Xcfd223, at distances of 3.7 and 1.7 cM, respectively, on the long arm of chromosome 3D. The Xgwm3 primers amplified codominant SSR alleles, a 72 bp fragment linked in coupling to the resistance allele and an 84 bp fragment linked in repulsion. Primers for the SSR Xcfd223 amplified a 153 bp fragment from the resistant Synthetic parent and a 183 bp fragment from the susceptible Opata line. Deletion mapping of the flanking Xgwm3 and Xcfd223 markers located them within the 3DL-3 deletion on the distal 19% of the long arm of chromosome 3D. This location is at least 20 cM proximal to the reported 3DL location of H24, a gene that confers resistance to Biotype D of the Hessian fly. Tight linkage of the markers will provide a means of detecting H32 presence in marker-assisted selection and gene pyramiding as an effective strategy for extending durability of deployed resistance. 相似文献
17.
Uttam Kumar Arun K. Joshi Sundeep Kumar Ramesh Chand Marion S. Röder 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(4):783-792
Spot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat in warm and humid wheat growing regions of the world. The development of disease resistant
cultivars is considered as the most effective control strategy for spot blotch. An intervarietal mapping population in the
form of recombinant inbred lines (RILs) was developed from a cross ‘Yangmai 6’ (a Chinese source of resistance) × ‘Sonalika’
(a spot blotch susceptible cultivar). The 139 single seed descent (SSD) derived F6, F7, F8 lines of ‘Yangmai 6’ × ‘Sonalika’ were evaluated for resistance to spot blotch in three blocks in each of the 3 years. Joint
and/or single year analysis by composite interval mapping (CIM) and likelihood of odd ratio (LOD) >2.2, identified four quantitative
trait loci (QTL) on the chromosomes 2AL, 2BS, 5BL and 6DL. These QTLs were designated as QSb.bhu-2A, QSb.bhu-2B, QSb.bhu-5B and QSb.bhu-6D, respectively. A total of 63.10% of phenotypic variation was explained by these QTLs based on the mean over years. Two QTLs
on chromosomes 2B and 5B with major effects were consistent over 3 years. All QTL alleles for resistance were derived from
the resistant parent ‘Yangmai 6’. 相似文献
18.
Resistance to frost and winter hardiness, as well as crop capacity and its components, were studied in the recombinant-inbred F5 Odesskaya and 16/Bezostayal lines and also in 61 lines of winter wheat carrying different alleles of the Rht8 gene. We observed no influence of different alleles of the Rht8 gene on the traits under consideration. The preponderance of the Rht8c allele in modern wheat lines developed at the Plant Breeding and Genetics Institute is therefore a result of its being the most frequently used allele in the genetic pool since 1959. 相似文献
19.
Marielle Merlino Philippe Leroy Christophe Chambon Gérard Branlard 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(7):1321-1337
Albumins and globulins of wheat endosperm represent 20% of total kernel protein. They are soluble proteins, mainly enzymes
and proteins involved in cell functions. Two-dimensional gel immobiline electrophoresis (2DE) (pH 4-7) × SDS-Page revealed
around 2,250 spots. Ninety percent of the spots were common between the very distantly related cultivars ‘Opata 85’ and ‘Synthetic
W7984’, the two parents of the International Triticeae Mapping Initiative (ITMI) progeny. ‘Opata’ had 130 specific spots while
‘Synthetic’ had 96. 2DE and image analysis of the soluble proteins present in 112 recombinant inbred lines of the F9-mapped
ITMI progeny enabled 120 unbiased segregating spots to be mapped on 21 wheat (Triticum aestivum L. em. Thell) chromosomes. After trypsic digestion, mapped spots were subjected to MALDI-Tof or tandem mass spectrometry for
protein identification by database mining. Among the ‘Opata’ and ‘Synthetic’ spots identified, many enzymes have already been
mapped in the barley and rice genomes. Multigene families of Heat Shock Proteins, beta-amylases, UDP-glucose pyrophosphorylases,
peroxydases and thioredoxins were successfully identified. Although other proteins remain to be identified, some differences
were found in the number of segregating proteins involved in response to stress: 11 proteins found in the modern selected
cultivar ‘Opata 85’ as compared to 4 in the new hexaploid `Synthetic W7984’. In addition, ‘Opata’ and ‘Synthetic’ differed
in the number of proteins involved in protein folding (2 and 10, respectively). The usefulness of the mapped enzymes for future
research on seed composition and characteristics is discussed.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
20.
The application of wheat microsatellites to identify disomic Triticum aestivum-Aegilops markgrafii addition lines 总被引:7,自引:0,他引:7
A. Peil V. Korzun V. Schubert E. Schumann W. E. Weber M. S. Röder 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):138-146
We describe the use of wheat microsatellites for the discrimination of Aegilops markgrafii chromosomes. Twenty out of eighty eight wheat microsatellites (WMS) tested were able to distinguish Triticum aestivum-Ae. markgrafii addition lines. Six, three, three, one and six of 18 WMS can be used as markers for single Ae. markgrafii chromosomes B, C, D, F and G, respectively. Addition line A is not available but additional bands, appearing only in Ae. markgrafii and the T. aestivum-Ae. markgrafii amphiploid and not in any of the available addition lines, indicate that three WMS detect markers for Ae. markgrafii chromosomes A. Addition line E could not be detected by any of the WMS markers applied, although the 20 WMS represented all
the homologous groups of wheat. All three WMS located on the short arm of group-2 chromosomes were located on Ae. markgrafii chromosome B; three of four WMS, located on the long arm of wheat group-2 chromosomes, were specific to Ae. markgrafii chromosome G and three of four WMS, specific to group-5 chromosomes, were markers for Ae. markgrafii chromosome C, indicating the homoeology of these wheat chromosome arms with the respective Ae. markgrafii chromosomes.
Received: 29 May 1997 / Accepted: 10 September 1997 相似文献