Effect of exogenous testosterone propionate & progesterone on testicular ascorbic acid & cholesterol in relation to spermatogenesis.

The effect of exogenous testosterone propionate (TP) and progesterone on testicular ascorbic acid and cholesterol in relation to spermatogenesis was investigated in rat, mouse, guinea pig, pigeon, and toad. TP and progesterone were injected at a dose of 4 mg/100 gm body weight. The results revealed a significant increase (p less than .001 or p less than .05) in testicular ascorbic acid and cholesterol levels after treatment in all species of animals except the toad where the cholesterol level decreased after progesterone treatment. Histologically, these hormones inhibited spermatogenesis in all species studied.     

Amperometric determination of serum total cholesterol with nanoparticles of cholesterol esterase and cholesterol oxidase

We describe the preparation of glutaraldehyde cross-linked and functionalized cholesterol esterase nanoparticles (ChENPs) and cholesterol oxidase nanoparticles (ChOxNPs) aggregates and their co-immobilization onto Au electrode for improved amperometric determination of serum total cholesterol. Transmission electron microscope (TEM) images of ChENPs and ChOxNPs showed their spherical shape and average size of 35.40 and 56.97 nm, respectively. Scanning electron microscope (SEM) studies of Au electrode confirmed the co-immobilization of enzyme nanoparticles (ENPs). The biosensor exhibited optimal response at pH 5.5 and 40 °C within 5 s when polarized at +0.25 V versus Ag/AgCl. The working/linear range of the biosensor was 10–700 mg/dl for cholesterol. The sensor showed high sensitivity and measured total cholesterol as low as 0.1 mg/dl. The biosensor was evaluated and employed for total cholesterol determination in sera of apparently healthy and diseased persons. The analytical recovery of added cholesterol was 90%, whereas the within-batch and between-batch coefficients of variation (CVs) were less than 2% and less than 3%. There was a good correlation (r = 0.99) between serum cholesterol values as measured by the standard enzymic colorimetric method and the current method. The initial activity of ENPs/working electrode was reduced by 50% during its regular use (200 times) over a period of 60 days when stored dry at 4 °C.