Fibronectin Regulates Wnt7a Signaling and Satellite Cell Expansion

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Highlights? Fzd7 and Sdc4 form a coreceptor complex in satellite cells ? FN stimulates Wnt7a activity through the Fzd7/Sdc4 coreceptor complex ? Activating satellite cells remodel their niche with FN ? FN regulates the satellite cell pool during muscle regeneration     

Stalled fork rescue via dormant replication origins in unchallenged S phase promotes proper chromosome segregation and tumor suppression

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Highlights? An unstable MCM2-7 complex results in a loss of dormant origins in Mcm4^Chaos3 cells ? A loss of dormant origins impairs stalled fork recovery in unchallenged S phase ? A loss of dormant origins increases replication intermediates in prophase ? Replication intermediates in M phase are a likely cause of chromosome instability     

Characterization and In Vivo Pharmacological Rescue of a Wnt2-Gata6 Pathway Required for Cardiac Inflow Tract Development

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Highlights? Wnt2 is required for atrial and inflow tract morphogenesis ? Wnt2 regulates expansion of secondary heart field progenitors ? Defects in Wnt2^?/? mutants can be rescued using Wnt signaling agonists ? Wnt2 cooperates with Gata6 to regulate cardiac inflow tract development     

Trachea-Derived Dpp Controls Adult Midgut Homeostasis in Drosophila

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Highlights? Trachea-derived Dpp/Bmp is required for midgut homeostasis ? Dpp expressed in tracheal cells reach intestinal cells through visceral muscles ? Dpp signaling is activated in enterocytes (ECs) in the Drosophila adult midgut ? Dpp signaling stabilizes ECs to restrict intestinal stem cell proliferation     

Tissue engineering 2.0: guiding self-organization during pluripotent stem cell differentiation

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Highlights? Self-organization is a property of developmental systems and pluripotent stem cells. ? Tissue engineering approaches can be applied to control self-organization in vitro. ? Maturation of pluripotent stem cells in vitro may be promoted by self-organization. ? Subtle, dynamic signals are provided to pluripotent cells through self-organization.     

Tmem64 Modulates Calcium Signaling during RANKL-Mediated Osteoclast Differentiation

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Highlights? Tmem64-deficient mice show increased bone volume ? Tmem64 deficiency reduces [Ca²⁺]_i oscillation in response to RANKL stimulation ? Tmem64 interacts with SERCA2 ? Tmem64 positively regulates osteoclast formation via SERCA2/Ca²⁺ signaling     

Identification of Distinct Ligands for the C-type Lectin Receptors Mincle and Dectin-2 in the Pathogenic Fungus Malassezia

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Highlights? Mincle and Dectin-2 cooperatively recognize Malassezia through distinct ligands ? The Mincle ligands in Malassezia are glucosyl- and mannosylglycolipids ? The Dectin-2 ligand in Malassezia is an O-linked mannobiose-rich glycoprotein ? Cytokine response to the respective ligands was impaired in Mincle^?/? and Dectin-2^?/? DCs     

Macrophage autophagy plays a protective role in advanced atherosclerosis

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Highlights? Proapoptotic oxidative stress induces autophagy in macrophages (M?s) ? Inhibition of autophagy enhances oxidative stress and apoptosis in M?s ? Atheromata of M?-Atg5^?/?Ldlr^?/? mice have increased apoptosis and plaque necrosis ? Apoptotic Atg5^?/? M?s are poorly recognized by phagocytes in vitro and in atheromata     

The metabolic profile of tumors depends on both the responsible genetic lesion and tissue type

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Highlights? MYC and MET affect glucose and glutamine metabolism differently in the liver ? Tissue context affects the outcome of metabolic reprogramming by MYC ? MYC overexpression sensitizes cells to inhibition of Gls1 glutaminase     

MicroRNA-133 Controls Brown Adipose Determination in Skeletal Muscle Satellite Cells by Targeting Prdm16

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Highlights? Satellite cells can differentiate into brown adipocytes ? MicroRNA-133 targets Prdm16 3′UTR and controls brown adipose determination ? MicroRNA-133 antagonism induces active brown adipocytes within regenerating muscle ? MicroRNA-133 antagonism impedes the development of obesity     

Interplay between the Dividing Cell and Its Neighbors Regulates Adherens Junction Formation during Cytokinesis in Epithelial Tissue

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Highlights? During cytokinesis, neighboring cells accumulate MyoII at the edges of the furrow ? MyoII nonautonomously sets the initial geometry of the daughter cell interface ? Neighboring membranes impede adherens junction (AJ) formation until a midbody forms ? Arp2/3-dependent actin accumulation in the dividing cell maintains AJ geometry     

The IRP1-HIF-2α Axis Coordinates Iron and Oxygen Sensing with Erythropoiesis and Iron Absorption

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Highlights? Derepression of HIF-2α mRNA in Irp1^?/? mice causes age-dependent polycythemia ? HIF-2α hyperactivity is observed in multiple tissues of Irp1^?/? mice ? The mRNA regulons of IRP1 and IRP2 are separable in vivo ? The IRP1-HIF-2α axis is a therapeutic target for hematologic or oncologic disorders     

Conformational Dynamics of the Rpt6 ATPase in Proteasome Assembly and Rpn14 Binding

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Highlights? Proteasome ATPase Rpt6 undergoes helix-coil exchange in its C-terminal domain ? Rpt6 G^360,387A has a stabilized four-helix bundle and raised melting temperature ? Assembly chaperone Rpn14 binds selectively to the four-helix bundle Rpt6 conformer ? Rpt6 G^360,387A (rpt6AA) is synthetically defective with an rpn14 null mutation     

bantam miRNA Promotes Systemic Growth by Connecting Insulin Signaling and Ecdysone Production

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Highlights? bantam is required in ecdysone-producing cells to promote body growth ? bantam inhibits ecdysone biosynthesis ? Insulin signaling regulates ecdysone production by repressing bantam activity ? Both the systemic and tissue-autonomous activities of bantam promote organ growth     

EBF2 Determines and Maintains Brown Adipocyte Identity

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Highlights? Ebf2 is selectively expressed in brown relative to white adipocytes and binds to chromatin at brown fat-specific target sites of Pparγ ? Ebf2 expression in white fat or muscle precursor cells recruits Pparγ to its brown fat-specific gene targets and drives a complete program of brown adipocyte differentiation ? Brown adipose cells and tissue from Ebf2-deficient mice display a complete loss of thermogenic characteristics while gaining molecular attributes of white adipose     

A subpopulation of adult skeletal muscle stem cells retains all template DNA strands after cell division

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Highlights? Pax7^Hi quiescent stem cells constitute a metabolically low “dormant” subpopulation ? Both Pax7^Hi and Pax7^Lo quiescent stem cells are serially transplantable ? Only proliferating Pax7^Hi cells segregate old DNA strands to the renewing stem cell ? CO-FISH demonstrates that template DNA strand cosegregation engages all chromosomes     

Generation of Rejuvenated Antigen-Specific T Cells by Reprogramming to Pluripotency and Redifferentiation

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Highlights? Reprogramming of antigen-specific T cells to generate iPSCs (T-iPSCs) ? Redifferentiation of CD8⁺ T cells, with original antigen specificity, from T-iPSCs ? Newly differentiated T cells show high proliferation and elongated telomeres ? T cell antigen-specific cytotoxicity is maintained     

Fertility and Germline Stem Cell Maintenance under Different Diets Requires nhr-114/HNF4 in C. elegans

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Highlights? C. elegans fertility on different diets requires nuclear receptor nhr-114 activity ? Somatic NHR-114 is necessary to maintain germline stem cells under different diets ? NHR-114 might mediate a detoxification response that preserves the germline ? Bacterial tryptophan metabolites may underlie diet-sensitive nhr-114(?) sterility