共查询到20条相似文献,搜索用时 31 毫秒
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RhoA activation promotes transformation and loss of thyroid cell differentiation interfering with thyroid transcription factor-1 activity. 总被引:1,自引:0,他引:1
Diego L Medina Marcos Rivas Patricia Cruz Isabel Barroso Javier Regadera Pilar Santisteban 《Molecular endocrinology (Baltimore, Md.)》2002,16(1):33-44
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Thyroglobulin repression of thyroid transcription factor 1 (TTF-1) gene expression is mediated by decreased DNA binding of nuclear factor I proteins which control constitutive TTF-1 expression 总被引:2,自引:0,他引:2
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Nakazato M Chung HK Ulianich L Grassadonia A Suzuki K Kohn LD 《Molecular and cellular biology》2000,20(22):8499-8512
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A M Musti V M Ursini E V Avvedimento V Zimarino R Di Lauro 《Nucleic acids research》1987,15(20):8149-8166
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Expression and function of the homeodomain-containing protein Hex in thyroid cells 总被引:4,自引:0,他引:4
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Pellizzari L D'Elia A Rustighi A Manfioletti G Tell G Damante G 《Nucleic acids research》2000,28(13):2503-2511
The homeodomain-containing protein Hex (also named Prh) is expressed in primitive endoderm (during the early phases of development), in some endoderm-derived tissues and in endothelial and hematopoietic precursors. Hex expression is extinguished during terminal differentiation of endothelial and hematopoietic cells as well as in adult lung. Previous investigations have demonstrated that Hex is expressed during early thyroid gland development. No information has been reported on Hex expression in adult thyroid gland or on the function of this protein in follicular thyroid cells. These issues represent the focus of the present study. We demonstrate that Hex mRNA is present in rat and human adult thyroid gland as well as in differentiated follicular thyroid cell lines. In FRTL-5 cells TSH reduces Hex expression. In thyroid cell lines transformed by several oncogenes Hex expression is completely abolished. By using co-transfection assays we demonstrate that Hex is a repressor of the thyroglobulin promoter and that it is able to abolish the activating effects of both TTF-1 and Pax8. These data would suggest that Hex may play an important role in thyroid cell differentiation. Protein–DNA interaction experiments indicate that Hex is able to bind sites of the thyroglobulin promoter containing either the core sequence 5′-TAAT-3′ or 5′-CAAG-3′. The DNA binding specificity of the Hex homeodomain, therefore, is more ‘relaxed’ than that observed in the majority of other homeodomains. 相似文献
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Thyroid nuclear factor 1 (TTF-1) contains a homeodomain and displays a novel DNA binding specificity. 总被引:32,自引:3,他引:29
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The cDNA for TTF-1, a thyroid nuclear factor that binds to the promoter of thyroid specific genes, has been cloned. The protein encoded by the cDNA shows binding properties indistinguishable from those of TTF-1 present in nuclear extracts of differentiated rat thyroid cells. The DNA binding domain of TTF-1 is a novel mammalian homeodomain that shows considerable sequence homology to the Drosophila NK-2 homeodomain. TTF-1 mRNA and corresponding binding activity are detected in thyroid and lung. The chromosomal localization of the TTF-1 gene has been determined in humans and mice and corresponds to chromosomes 14 and 12, respectively, demonstrating that the TTF-1 gene is not located within previously described clusters of homeobox-containing genes. 相似文献
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Protein binding domains of the rat thyroglobulin promoter 总被引:2,自引:0,他引:2
M V Ursini A Gallo E Olivetta A M Musti 《Biochemical and biophysical research communications》1989,163(1):481-488
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