共查询到20条相似文献,搜索用时 15 毫秒
1.
Yanliu Yang Gaiyun Zhang Zhilei Sun Man Kit Cheung Cheney Huang 《Antonie van Leeuwenhoek》2014,106(6):1191-1198
A Gram-stain negative, ovoid-rod shaped, strictly aerobic bacterium, strain Y2T, was isolated from a deep-sea sediment of the Western Pacific. Phylogenetic and phenotypic properties of the organism indicated that it belongs to the genus Altererythrobacter. Strain Y2T shares highest 16S rRNA gene sequence similarity of 96.6 % with Erythrobacter jejuensis CNU001T, followed by the type strains of recognized members of the genus Altererythrobacter (94.8–96.5 %). Strain Y2T forms a clade with E. jejuensis CNU001T in the cluster of species of the genus Altererythrobacter. Growth of strain Y2T was observed at 4–40 °C (optimum, 35–37 °C), at pH 6.0–10.0 (optimum, pH 7.0–8.0) and in the presence of 0–5 % (w/v) NaCl (optimum, 2–3 %). The major cellular fatty acids were found to be C17:1 ω6c (41.5 %), summed feature 8 (C18:1 ω7c and/or C18:1 ω6c; 17.2 %), C17:1 ω8c (11.0 %) and C15:0 2OH (8.1 %). The major respiratory quinone was determine to be ubiquinone 10 (Q-10). The polar lipid analysis indicated the presence of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, one sphingoglycolipid, three unidentified phospholipids, two unidentified glycolipids, two unidentified aminolipids and three unknown lipids. The DNA G + C content of the type strain is 60.0 mol %. On the basis of the data from the polyphasic characterization, strain Y2T represents a novel species, for which the name Altererythrobacter oceanensis sp. nov. is proposed. The type strain is Y2T (=CGMCC 1.12752T =LMG 28109T). 相似文献
2.
Mariyam Shahina Asif Hameed Shih-Yao Lin Wei-An Lai Yi-Han Hsu Chiu-Chung Young 《Antonie van Leeuwenhoek》2014,106(5):1031-1040
3.
Qipei Li Yongle Xu Keshao Liu Lanlan Cai Yingnan Fu Jia Sun Rui Zhang 《Current microbiology》2013,66(5):450-455
A Gram-negative, rod-shaped, non-spore-forming aerobic bacterium, motile with a single polar flagellum, strain JLT2005T, was isolated from surface seawater collected from the East China Sea and formed ivory white colonies on a rich organic medium. The strain was positive for catalase, oxidase, and urease. It grew in the presence of 0–12 % (w/v) NaCl (optimum 5 %), at 20–35 °C (optimum 25 °C), or at pH 6–10 (optimum pH 9). The major fatty acids (>10 %) were C18:1ω7c, C19:0ω8c cyclo, C16:0, and C18:0. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, and five unidentified glycolipids. Ubiquinone-10 and Ubiquinone-11 were present as the major quinones. The DNA G+C content was 74.3 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain JLT2005T belongs to the genus Pelagibacterium in the family Hyphomicrobiaceae, class Alphaproteobacteria. The closest neighbors were Pelagibacterium halotolerans B2T (98.7 % similarity) and Pelagibacterium luteolum 1_C16_27T (97.1 % similarity). DNA–DNA relatedness values of strain JLT2005T with P. halotolerans B2T and with P. luteolum 1_C16_27T were 31.6 and 25 %. Evidence from genotypic, chemotaxonomic, and phenotypic data shows that strain JLT2005T represents a novel species of the genus Pelagibacterium, for which the name Pelagibacterium nitratireducens sp. nov is proposed. The type strain is JLT2005T (=CGMCC 1.10829T =JCM 17767T). 相似文献
4.
Zhen Yu Ming Chang Min Wu Guiqin Yang Shungui Zhou Li Zhuang 《Archives of microbiology》2013,195(12):815-822
A Gram-staining-negative, rod-shaped and motile with several polar flagellums bacterium, designated WM-3T, was isolated from a rice paddy soil in South China. Growth occurred with 0–3.0 % (w/v) NaCl (optimum 2.0 %), at pH 5.5–9.0 (optimum pH 7.0) and at 25–42 °C (optimum 30–37 °C) in liquid Reasoner’s 2A medium. Analysis of the 16S rRNA gene and gyrB gene sequences revealed that strain WM-3T was most closely related to the type strains of the species Pseudomonas linyingensis and Pseudomonas sagittaria. Its sequence similarities with P. linyingensis CGMCC 1.10701T and P. sagittaria JCM 18195T were 97.4 and 97.3 %, respectively, for 16S rRNA gene, and were 94.1 and 94.2 %, respectively, for gyrB gene. DNA–DNA hybridization between strain WM-3T and these two type strains showed relatedness of 35.6 and 30.9 %, respectively. G+C content of genomic DNA was 69.4 mol%. The whole-cell fatty acids mainly consisted of C16:0 (30.0 %), C16:1 ω6c and/or C16:1 ω7c (19.3 %) and C18:1 ω6c and/or C18:1 ω7c (16.3 %). The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain WM-3T belongs to genus Pseudomonas but represents a novel species, for which the name Pseudomonas oryzae sp. nov. is proposed. The type strain is WM-3T (=KCTC 32247T =CGMCC 1.12417T). 相似文献
5.
Sooyeon Park Soo-In Kim Chul-Hyung Kang Ji-Min Park Jung-Hoon Yoon 《Antonie van Leeuwenhoek》2014,106(5):927-934
A Gram-stain negative, aerobic, motile and rod-shaped bacterial strain, designated J-MY2T, was isolated from a tidal flat sediment of the South Sea, South Korea. Strain J-MY2T was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain J-MY2T forms a cluster with the type strains of Simiduia species. Strain J-MY2T exhibited 16S rRNA gene sequence similarity values of 97.62–98.77 % to the type strains of four Simiduia species and of <92.95 % sequence similarity to the type strains of the other recognized species. Strain J-MY2T was found to contain Q-8 as the predominant ubiquinone and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:0, C18:1 ω7c and C17:1 ω8c as the major fatty acids. The major polar lipids of strain J-MY2T were identified as phosphatidylethanolamine, phosphatidylglycerol, three unidentified glycolipids and one unidentified lipid. The DNA G+C content of strain J-MY2T was determined to be 54.8 mol% and its mean DNA–DNA relatedness values with the type strains of the four Simiduia species were in the range 21–34 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain J-MY2T is separated from other Simiduia species. On the basis of the data presented, strain J-MY2T is considered to represent a novel species of the genus Simiduia, for which the name Simiduia aestuariiviva sp. nov. is proposed. The type strain is J-MY2T ( = KCTC 42073T = CECT 8571T). 相似文献
6.
Guizhen Li Qiliang Lai Xiupian Liu Fengqin Sun Zongze Shao 《Antonie van Leeuwenhoek》2014,105(5):863-869
A taxonomic study was carried out on strain 22II-S10sT, which was isolated from the surface seawater of the Atlantic Ocean. The bacterium was found to be Gram-negative, oxidase and catalase positive, rod shaped and motile by subpolar flagella. The isolate was capable of gelatine hydrolysis but unable to reduce nitrate to nitrite or degrade Tween 80 or aesculin. Growth was observed at salinities of 0.5–18 % (optimum, 2–12 %), at pH of 3–10 (optimum, 7) and at temperatures of 10–41 °C (optimum 28 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S10sT belongs to the genus Roseivivax, with highest sequence similarity to Roseivivax halodurans JCM 10272T (97.2 %), followed by Roseivivax isoporae LMG 25204T (97.0 %); other species of genus Roseivivax shared 95.2–96.7 % sequence similarity. The DNA–DNA hybridization estimate values between strain 22II-S10sT and the two type strains (R. halodurans JCM 10272T and R. isoporae LMG 25204T) were 22.00 and 21.40 %. The principal fatty acids were identified as Summed Feature 8 (C18:1 ω7c/ω6c) (67.4 %), C18:0 (7.2 %), C19:0 cyclo ω8c (7.1 %), C18:1 ω7c 11-methyl (6.8 %) and C16:0 (5.9 %). The respiratory quinone was determined to be Q-10 (100 %). Phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an aminolipid, a glycolipid and three phospholipids were present. The G+C content of the chromosomal DNA was determined to be 67.5 mol%. The combined genotypic and phenotypic data show that strain 22II-S10sT represents a novel species within the genus Roseivivax, for which the name Roseivivax atlanticus sp. nov. is proposed, with the type strain 22II-S10sT (= MCCC 1A09150T = LMG 27156T). 相似文献
7.
Shih-Yao Lin Asif Hameed Fo-Ting Shen You-Cheng Liu Yi-Han Hsu Mariyam Shahina Wei-An Lai Chiu-Chung Young 《Antonie van Leeuwenhoek》2014,105(6):1149-1162
A taxonomic study was carried out on a novel aerobic bacterial strain (designated CC-LY736T) isolated from a fermentor in Taiwan. Cells of strain CC-LY736T were Gram-stain negative, spiral-shaped and motile by means of a monopolar flagellum. Strain CC-LY736T shared the greatest degree of 16S rRNA gene sequence similarity to Azospirillum irakense DSM 11586T (97.2 %), Rhodocista centenaria JCM 21060T (96.3 %) and Rhodocista pekingensis JCM 11669T (96.1 %). The major fatty acids were C16:0, C16:1 ω5c, C19:0 cyclo ω8c, C18:1 ω7c/C18:1 ω6c, C16:0 3-OH and C18:1 2-OH. The predominant polar lipids included phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidyldimethylethanolamine and two unidentified glycolipids. The common major respiratory quinone was ubiquinone Q-10 and predominant polyamines were sym-homospermidine and putrescine. The DNA G+C content of strain CC-LY736T was 67.6 ± 0.1 mol %. During phylogenetic analysis, strain CC-LY736T formed a unique phyletic lineage associated with Rhodocista species. However, the combination of genetic, chemotaxonomic and physiological data clearly indicated that strain CC-LY736T was a novel representative of the family Rhodospirillaceae. Based on the polyphasic comparison, the name Niveispirillum fermenti gen. nov., sp. nov. is proposed; the type strain of the type species is CC-LY736T (= BCRC 80504T = LMG 27263T). In addition, the reclassifications of Azospirillum irakense as Niveispirillum irakense comb. nov. (type strain KBC1T = ATCC 51182T = BCRC 15764T = CIP 103311T), and Azospirillum amazonense as Nitrospirillum amazonense gen. nov., sp. nov. (type strain Am14T = ATCC 35119T = BCRC 14279T = DSM 3787T) are proposed based on the polyphasic taxonomic data obtained in this study. 相似文献
8.
Yung Mi Lee Chung Yeon Hwang Inae Lee You-Jung Jung Yirang Cho Kiwoon Baek Soon Gyu Hong Ji-Hee Kim Jongsik Chun Hong Kum Lee 《Antonie van Leeuwenhoek》2014,106(3):527-533
A Gram-negative, strictly aerobic, non-motile, rod-shaped and psychrophilic bacterial strain, PAMC 27137T, was isolated from the marine sediment of the Ross Sea, Antarctica. Strain PAMC 27137T was observed to grow at 4–10 °C, at pH 6.5–7.5 and in the presence of 2.5–4.0 % (w/v) sea salts. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PAMC 27137T belongs to the genus Lacinutrix showing the high similarities with Lacinutrix mariniflava JCM 13824T (97.6 %) and Lacinutrix algicola JCM 13825T (97.1 %). Genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strain PAMC 27137T is clearly distinguished from the most closely related Lacinutrix species. The major fatty acids (>5 %) were identified as iso-C15:1 G (19.9 %), iso-C15:0 (19.3 %), iso-C17:0 3-OH (11.3 %), summed feature 9 (C16:0 10-methyl and/or iso-C17:1 ω9c as defined by MIDI, 9.1 %), iso-C15:0 3-OH (7.5 %), and anteiso-C15:1 A (5.8 %). The polar lipids were found to consist of phosphatidylethanolamine, an unidentified aminolipid, an unidentified aminophospholipid, and five unidentified phospholipids. The major respiratory quinone was identified as MK-6. The genomic DNA G+C content was determined to be 32.1 mol%. Based on the data from this polyphasic taxonomic study, strain PAMC 27137T is considered to represent a novel species of the genus Lacinutrix, for which the name Lacinutrix jangbogonensis sp. nov. is proposed. The type strain is PAMC 27137T (=KCTC 32573T=JCM 19883T). 相似文献
9.
A novel pale pink-coloured, strictly aerobic, Gram-stain negative bacterial strain, designated strain KER25-12T, was isolated from a laboratory air-conditioning system in South Korea. Cells were observed to be non-motile cocci showing positive catalase and oxidase reactions. Strain KER25-12T was found to grow at 10–30 °C (optimum, 25–30 °C), at pH 4.0–9.0 (optimum, pH 6.0–7.0) and in the presence of 0–2% (w/v) NaCl (optimum, 0%). Ubiquinone-10 and spermidine were detected as the sole respiratory quinone and the predominant polyamine, respectively. The major fatty acids were identified as summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c), summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), C16:0 and C18:0. The genomic DNA G+C content of strain KER25-12T was determined to be 70.0 mol%. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unidentified aminolipid. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain KER25-12T belongs to the genus Roseomonas and shows high sequence similarity to Roseomonas aerilata 5420S-30T (98.57%), Roseomonas pecuniae N75T (97.44%) and Roseomonas vinacea CPCC 100056T (97.40%). Based on the morphological, physiological, chemotaxonomic and phylogenetic features, strain KER25-12T is concluded to represent a novel species of the genus Roseomonas, for which the name Roseomonas aeriglobus sp. nov. is proposed. The type strain is KER25-12T (= KACC 19282T = JCM 32049T). 相似文献
10.
Yi Li Hong Zhu Qiliang Lai Xueqian Lei Huajun Zhang Zhangran Chen Wei Zheng Yun Tian Hong Xu Tianling Zheng 《Antonie van Leeuwenhoek》2014,106(5):911-918
A Gram-stain negative, short rod-shaped aerobic bacterium with flagella, designated strain Y32T, was isolated from coastal seawater in Xiamen, Fujian Province of China. 16S rRNA gene sequence comparisons showed that strain Y32T is a member of the family Oceanospirillaceae, forming a distinct lineage with species of the genus Litoribacillus. The 16S rRNA gene sequence similarities between strain Y32T and other strains were all less than 94.0 %. Strain Y32T was found to grow optimally at 28 °C, at pH 7.0–8.0 and in the presence of 4–5 % (w/v) NaCl. The major fatty acids were identified as Summed Feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c, 49.4 %), C16:0 (17.7 %), C14:0 (6.9 %) and C18:1 ω9c (5.4 %). The major respiratory quinone was identified as ubiquinone-8 (Q-8). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain Y32T was determined to be 55.6 mol%. According to its morphology, physiology, fatty acid composition, polar lipids composition and 16S rRNA gene sequence data, strain Y32T represents a novel species of a new genus in the family Oceanospirillaceae, for which the name Litoribrevibacter albus gen. nov. sp. nov. is proposed. The type strain of Litoribrevibacter albus is Y32T (=MCCC 1F01211T=NBRC 110071T). 相似文献
11.
Yanfeng Zhang Yong Xu Wei Fang Xiaotang Wang Zemin Fang Yazhong Xiao 《Antonie van Leeuwenhoek》2017,110(2):187-194
A taxonomic study was carried out on a Gram-stain negative, rod-shaped, non-flagellated, and facultatively anaerobic bacterial strain designated as strain SF-16T, which was isolated from an unidentified starfish in Sanya, China. Strain SF-16T was found to be 5.0–7.0 μm long, and oxidase and catalase positive. Cell growth was observed at pH 6.0–8.5 (optimum, 7.0–8.0), temperatures of 10–41 °C (optimum, 25–30 °C), and salinities of 0–12 % (optimum, 3.0–6.0 %). The predominant fatty acids (>20 %) were found to be C18:1 ω7c and/or C18:1 ω6c (summed feature 8). Ubiquinone 10 was identified as the predominant quinone for strain SF-16T. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, two unidentified lipids, and three unidentified phospholipids. The DNA G+C content of the genomic DNA of strain SF-16T was determined to be 63.0 mol%. Phylogenetic analysis based on the 16S rRNA gene showed that strain SF-16T belongs to the genus Pseudoruegeria and is closely related to Pseudoruegeria sabulilitoris GJMS-35T (98.42 % similarity). The ANI value between strain SF-16T and P. sabulilitoris GJMS-35T was found to be 74.98 %, and DNA–DNA hybridization value was 21.1 ± 2.3 % in silico and 57 % in vitro. Based on the low level of the genetic relatedness, phylogenetic and phenotypic data, a novel species Pseudoruegeria marinistellae sp. nov. is proposed. The type strain is SF-16T (=MCCC 1K01155T = KCTC 42910T). 相似文献
12.
Jae-Jin Lee Yeon-Hee Lee Su-Jin Park Seung-Yeol Lee Sangkyu Park Dae Sung Lee In-Kyu Kang Leonid N. Ten Hee-Young Jung 《Antonie van Leeuwenhoek》2017,110(3):407-414
Strain 16F3HT, a Gram-negative, aerobic, non-motile, and oval-shaped bacterium, was isolated from river water collected from the Han River in South Korea. Growth of strain 16F3HT was observed at 10–42 °C (optimum at 25–30 °C), but no growth occurred at 4 °C. The strain is able to grow at pH 4–10 (optimum at pH 7–8) and tolerates up to 4% NaCl (w/v), with optimum growth at 0.5% NaCl. The isolate was found to be resistant to UV irradiation. Based on 16S rRNA gene sequence analysis, it is closely related to ‘Deinococcus seoulensis’ 16F1E (98.8%), Deinococcus aquaticus PB314T (98.1%) and Deinococcus caeni Ho-08T (98.0%). The level of DNA–DNA homology between the novel strain and the three related strains was 57.4, 41.2, and 35.8%, respectively. Chemotaxonomic data revealed that strain 16F3HT possesses MK-8 as the predominant respiratory quinone, an unidentified phosphoglycolipid as the major polar lipid, and C15:1 ω6c and C16:1 ω7c as the major fatty acids. The DNA G + C content was determined to be 65.7 mol%. Based on polyphasic evidence, strain 16F3HT (=KCTC 33794T = JCM 31406T) should be classified as the type strain of a novel Deinococcus species, for which the name Deinococcus knuensis sp. nov. is proposed. 相似文献
13.
T.N.R. Srinivas Y. Vijaya Bhaskar V. Bhumika P. Anil Kumar 《Systematic and applied microbiology》2013
The novel, cream colored, Gram-staining-negative, rod-shaped, motile bacteria, designated strains AK15T and AK18, were isolated from sediment samples collected from Palk Bay, India. Both strains were positive for arginine dihydrolase, lysine decarboxylase, oxidase, nitrate reduction and methyl red test. The major fatty acids were C16:0, C18:1 ω7c, C16:1 ω7c and/or C16:1 ω6c and/or iso-C15:0 2-OH (summed feature 3). Polar lipids content of strains AK15T and AK18 were found to bephosphatidylethanolamine (PE), two unidentified phospholipids (PL1 and PL2) and three unidentified lipids (L1-L3). The 16S rRNA gene sequence analysis indicated strains AK15T and AK18 as the members of the genus Photobacterium and closely related to the type strain Photobacterium jeanii with pair-wise sequence similarity of 96.7%. DNA–DNA hybridization between strain AK15T and AK18 showed a relatedness of 87%. Based on data from the current polyphasic study, strains AK15T and AK18 are proposed as novel species of the genus Photobacterium, for which the name Photobacterium marinum sp. nov. is proposed. The type strain of Photobacterium marinum is AK15T (=MTCC 11066T = DSM 25368T). 相似文献
14.
Xiuhua Bai Qiliang Lai Chunming Dong Fuying Li Zongze Shao 《Antonie van Leeuwenhoek》2014,106(3):449-455
A taxonomic study was carried out on strain D104T, which was isolated from deep-sea subsurface sediment sample from the Arctic Ocean. The bacterium was found to be Gram-negative, oxidase negative and catalase weakly positive, rod shaped, motile by means of polar flagellum. The organism grows between 4 and 37 °C (optimum 25–28 °C) and 0.5–6 % NaCl (optimum 3 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D104T belongs to the genus Marinomonas, with highest sequence similarities of 97.7 % to Marinomonas ushuaiensis DSM 15871T, followed by M. dokdonensis DSW10-10T (96.9 %), M. arenicola KMM 3893T (96.7 %), M. arctica 328T (96.6 %) and other 18 species of the genus Marinomonas (94.4–96.5 %). The average nucleotide identity and estimated DNA–DNA hybridization values between strain D104T and M. ushuaiensis DSM 15871T were 84.24 % and 20.80 ± 2.33 % respectively. The principal fatty acids were C16:0, sum in feature 3 (C16:1 ω7c/C16:1 ω6c), sum in feature 8 (C18:1 ω7c/C18:1 ω6c) and C12:1 3OH. The G + C content of the chromosomal DNA was determined to be 44.8 mol%. The respiratory quinone was found to be Q8 (100 %). Polar lipids include phosphatidylglycerol and phosphatidylethanolamine as major phospholipids and aminolipid and phospholipid as minor components. The results of the genotypic and phenotypic analyses indicate that strain D104T represents a novel species of the genus Marinomonas, for which the name Marinomonas profundimaris sp. nov. is proposed, with the type strain D104T (=MCCC 1A07573T = LMG 27696T). 相似文献
15.
Nupur Bhumika Vaidya Naga Radha Srinivas Tanuku Anil Kumar Pinnaka 《Antonie van Leeuwenhoek》2013,103(2):347-355
A novel marine, Gram-negative, rod-shaped bacterium, designated strain N9T, was isolated from a water sample of the sea shore at Visakhapatnam, Andhra Pradesh (India). Strain N9T was found to be positive for oxidase and catalase activities. The fatty acids were found to be dominated by C16:0, C18:1 ω7c and summed in feature 3 (C16:1 ω7c and/or C16:1 ω6c). Strain N9T was determined to contain Q-10 as the major respiratory quinone and phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, two phospholipids and four unidentified lipids as polar lipids. The DNA G+C content of the strain N9T was found to be 63 mol%. 16S rRNA gene sequence analysis indicated that Rhodobacter sphaeroides, Rhodobacter johrii, Pseudorhodobacter ferrugineus, Rhodobacter azotoformans, Rhodobacter ovatus and Pseudorhodobacter aquimaris were the nearest phylogenetic neighbours, with pair-wise sequence similarities of 95.43, 95.36, 94.24, 95.31, 95.60 and 94.74 %, respectively. Phylogenetic analysis showed that strain N9T formed a distinct branch within the family Rhodobacteraceae and clustered with the clade comprising species of the genus Pseudorhodobacter, together with species of the genera Roseicitreum, Roseinatronobacter, Roseibaca and Rhodobaca. Species of the genus Pseudorhodobacter are phylogenetically close with a 16S rRNA gene sequence dissimilarity of 5.9–7.3 % (92.7–94.1 % similarity). Based on the above-mentioned phenotypic characteristics and on phylogenetic inference, strain N9T is proposed as a representative of a new genus and a novel species of the family Rhodobacteraceae as Albirhodobacter marinus gen. nov., sp. nov. The type strain of Albirhodobacter marinus is N9 (= MTCC 11277T = JCM 17680T). 相似文献
16.
Yan Wang Rui Zhao Shiqi Ji Zhao Li Tong Yu Bingyu Li Xiaochong Shi Xiao-Hua Zhang 《Antonie van Leeuwenhoek》2013,104(3):423-430
A Gram-negative, non-spore-forming, catalase- and oxidase-positive, aerobic, curved-rod shaped bacterium with polar or subpolar flagellum, designated strain JYr2T, was isolated from a sediment sample collected from an amphioxus breeding zone in the coastal region of Qingdao, China. The organism grew optimally at 37 °C, pH 8–9 and in the presence of 3 % (w/v) NaCl or 3–4 % sea salts (w/v; Sigma). Salt was required for growth. The strain contained isoprenoid quinone 8 (Q-8, 100 %) as the predominant isoprenoid quinone and C16:0 (24.1 %) and C16:1 ω7c and/or C16:1 ω6c (35.8 %) as major fatty acids. Phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid (PN) and two aminolipid (AL1, AL2) were the major constituents of the phospholipids. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JYr2T formed a distinct evolutionary lineage within the family Colwelliaceae. It showed <95.0 % sequence similarities to all validly published species in the family Colwelliaceae, except 95.2 % to Thalassomonas viridans DSM 13754T and 95.0 % to Colwellia polaris JCM 537T. The G+C content of the DNA was 42.7 mol%. On the basis of the polyphasic taxonomic study, strain JYr2T (= CGMCC 1.10794T = JCM 17549T) was considered to represent a novel genus and species in Gammaproteobacteria, for which the name Litorilituus sediminis gen. nov. sp. nov. was proposed. 相似文献
17.
A Gram-stain negative, short rod-shaped, non-motile, catalase- and oxidase-positive, aerobic bacterium, designated F14T, was isolated from the Western Pacific Ocean. Phylogenetic and phenotypic properties of the organism supported that it belongs to the genus Paracoccus. The levels of 16S rRNA gene sequences similarity between strain F14T and other type strains of recognized members of the genus Paracoccus were 93.6–96.5 %. Growth of strain F14T was observed at 4–40 °C (optimum, 28–30 °C), pH 6.0–10.0 (optimum, pH 7.0–8.0) and in the presence of 0–7 % (w/v) NaCl (optimum, 1–2 %). The major cellular fatty acid was summed feature 8 (C18:1 ω6c and/or C18:1 ω7c). The major respiratory quinone was ubiquinone-10. The polar lipid pattern indicated the presence of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and three unknown lipids. The DNA G+C content was 61.4 mol%. On the basis of polyphasic characterization, strain F14T represents a novel species, for which the name Paracoccus pacificus sp. nov. is proposed. The type strain is F14T (=CGMCC 1.12755T=LMG 28106T=MCCC 1A09947T). 相似文献
18.
The strain designated as AB21T was isolated from chloroethylenes contaminated soil. Cells are gram-negative, aerobic, non-spore-forming, and motile rods. Phylogenetic analysis based on 16S rRNA gene sequence showed that it belonged to the genus Rhizobium, and was closely related to Rhizobium sullae IS 123T (97.4 %), Rhizobium yanglingense SH 22623T (97.2 %), Rhizobium gallicum R 602spT (97.1 %), Rhizobium alamii GBV 016T (97.0 %), and Rhizobium monogolense USDA 1844T (97.0 %). It showed less than 97 % identity with the remaining Rhizobium species. This novel isolate grew optimally at 25–37 °C (optimum, 30 °C) and pH 6–9 (optimum, pH 8.0). It grew in the presence of 0–4 % (w/v) NaCl, tolerating a 4 % (w/v) NaCl. DNA–DNA hybridization experiment shows less than 53 % binding with closely related Rhizobium. Predominant quinone is ubiquinone (Q-10). The major fatty acids were summed feature 8 (composed of C18:1 ω7c/C18:1 ω6c), C19:0 cyclo ω8c, and C16:0. The G+C molar content is 62.5 mol%. Based on the polyphasic analysis, strain AB21T is referred to be a novel species of the genus Rhizobium for which the name Rhizobium halotolerans sp. nov. is proposed. The type strain is AB21T (=KEMC 224-056T = JCM 17536T). 相似文献
19.
A Gram-stain negative, coccoid- or oval-shaped and non-gliding bacterial strain, designated CDM-17T, was isolated from the zone where the ocean and a freshwater spring meet at Jeju island, South Korea, and subjected to a polyphasic taxonomic study. Strain CDM-17T was observed to form smooth, circular, glistening, slightly convex, light yellowish pink colonies on marine agar, and was found to grow optimally at pH 7.0–8.0, at 30 °C and in the presence of 2–3 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain CDM-17T fell within the clade comprising the Roseivivax species, clustering with the type strain of Roseivivax sediminis with which it exhibited 98.3 % sequence similarity value. Sequence similarities to the type strains of the other recognized Roseivivax species were 94.7–96.8 %. Strain CDM-17T was found to contain Q-10 as the predominant ubiquinone and summed feature 8 (C18:1 ω6c and/or C18:1 ω7c) as the major fatty acid. The major polar lipids were identified as phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid and an unidentified lipid. The DNA G+C content of strain CDM-17T was determined to be 66.2 mol% and its mean DNA–DNA relatedness value with Rsv. sediminis KCTC 23444T was 17.5 ± 2.7 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain CDM-17T is distinguishable from recognized Roseivivax species. On the basis of the data presented, strain CDM-17T is proposed to represent a novel species of the genus Roseivivax, for which the name Roseivivax jejudonensis sp. nov. is proposed. The type strain is CDM-17T (=KCTC 42110T = CECT 8625T). 相似文献
20.
Jong-Sik Jin Keun Chul Lee In-Soon Park Kwang Kyu Kim Jong Seog Ahn Yoshimi Benno Masao Hattori Jung-Sook Lee 《Antonie van Leeuwenhoek》2014,106(3):439-447
A novel actinobacterial strain, designated CAT-2T, was isolated from human faeces as a bacterium capable of dehydroxylating (+)-catechin derivatives. Strain CAT-2T was found to be strictly anaerobic, Gram-positive, non-motile and non-spore-forming coccobacilli. The major fatty acids were identified as C16:0 DMA (dimethy acetal), C16:0, C14:0, anteiso-C15:0 and iso-C14:0. The three predominant menaquinones were identified as MK-6 (menaquinene-6), MMK-6 (monomethylmenaquinone-6) and DMMK-6 (dimethylmenaquinone-6). The polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol and four unidentified glycolipid. The DNA G+C content of strain CAT-2T was 68.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain CAT-2T belongs to the genus Gordonibacter, sharing the highest level of sequence homology with Gordonibacter pamelaeae DSM 19378T (97.3 %). Combined phenotypic, chemotaxonomic and phylogenetic characteristics support the conclusion that the strain CAT-2T represents a novel species, for which the name Gordonibacter faecihominis sp. nov. is proposed. The type strain is CAT-2T (= KCTC 15204T = JCM 16058T). 相似文献