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1.
Journal of Microbiology - Two bacterial strains designated NKC220-2T and NKC851-2 were isolated from commercial kimchi from different areas in Korea. The strains were Gram-positive, aerobic,... 相似文献
2.
Two novel Gram-negative, rod-shaped bacterial strains BT702T and BT704T were isolated from soil collected in Jeongseon (37° 22′ 45″ N, 128° 39′ 53″ E), Gangwon province, South Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT702T and BT704T belong to distinct lineage within the genus Spirosoma (family Cytophagaceae, order Cytophagales, class Cytophagia and phylum Bacteroidetes). The strain BT702T was closely related to Spirosoma flavus 15J11-2T (96.7% 16S rRNA gene similarity) and Spirosoma metallilatum TX0405T (93.3%). The strain BT704T was closely related to Spirosoma koreense 15J8-5T (94.6%), Spirosoma endophyticum DSM 26130T (93.8%) and Spirosoma humi S7-4-1T (93.8%). The genome sizes of type strains BT702T and BT704T are 8,731,341 bp and 8,221,062 bp, respectively. The major cellular fatty acids of strains BT702T and BT704T were C16:1 ω5c and summed feature 3 (C16:1 ω6c/C16:1 ω7c). The strains were found to have the same quinone system, with MK-7 as the major respiratory quinone. The major polar lipids of strain BT702T was identified to be phosphatidylethanolamine (PE), aminophospholipid (APL) and aminolipid (AL), while that of strain BT704T consisted of phosphatidylethanolamine (PE) and aminophospholipid (APL). Based on the polyphasic analysis (phylogenetic, chemotaxonomic and biochemical), strains BT702T and BT704T can be suggested as two new bacterial species within the genus Spirosoma and the proposed names are Spirosoma profusum and Spirosoma validum, respectively. The type strain of Spirosoma profusum is BT702T (=?KACC 22028T?=?NBRC 114859T) and type strain of Spirosoma validum is BT704T (=?KACC 22030T?=?NBRC 114966T). 相似文献
3.
Strain HME6451 T was isolated from Lake Soyang in Korea. Phylogenetic tree based on 16S rRNA gene sequence showed that strain HME6451 T formed a lineage within the genus Pedobacter. The strain HME6451 T was closely related to Pedobacter daechungensis (95.4% sequence similarity), Pedobacter lentus (94.4%), and Pedobacter glucosidilyticus (93.8%). And strain HME6451 T was a Gram-staining-negative, short rod-shaped, strictly aerobic bacterium. The major fatty acids were iso-C 15:0 (41.2%), summed feature 3 (comprising C 16:1 ω7 c and/or C 16:1 ω6 c; 23.1%), and iso-C 17:0-3OH (10.1%). The polar lipids of HME6451 T were consisted of one phosphatidylethanolamine, one unidentified aminolipid, one unidentified phospholipid and four unidentified polar lipids. The DNA G+C content was 36.0 mol%. On the basis of the evidence presented in this study, strain HME6451 T represent a novel species of the genus Pedobacter, for which the name Pedobacter soyangensis sp. nov., is proposed the type strain HME6451 T (=KCTC 23467 T =CECT 7865 T). 相似文献
4.
A Gram-staining positive, facultative aerobic bacterium, designated strain RH-N24 T, was isolated from naked barley in South Korea. Cells of the isolate were observed to be motile rods by means of peritrichous flagella and showed catalase-positive and oxidase-negative reactions. Growth of strain RH-N24 T was observed at 4–40 °C (optimum: 35–37 °C) and at pH 5.0–9.0 (optimum: pH 6.0–7.0). Chemotaxonomic data (major isoprenoid quinone: MK-7; DNA G + C content: 53.5 mol %; cell wall type: A1γ-meso-diaminopimelic acid; major fatty acids: anteiso-CB 15:0 and CB 16:0B) supported the affiliation of the isolate to the genus Paenibacillus. The major cellular polar lipids were identified as phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unidentified polar lipids. Phylogenetic analysis based on 16S rRNA gene sequences also supported the conclusion that strain RH-N24 T belonged to the genus Paenibacillus. Comparative 16S rRNA gene sequence analysis showed that strain RH-N24 T was most closely related to Paenibacillus hunanensis FeL05 T and Paenibacillus illinoisensis NRRL NRS-1356 T with similarities of 94.64 and 94.54 %, respectively. On the basis of phenotypic and molecular properties, strain RH-N24 T represents a novel species within the genus Paenibacillus for which the name Paenibacillus hordei sp. nov. is proposed. The type strain is RH-N24 T (=KACC 15511 T = JCM 17570 T). 相似文献
5.
A Gram-negative, short, rod-shaped bacterium, TSA1 T, was isolated from forest soil collected at Kyonggi University, South Korea. Assessment of 16S rRNA gene sequence similarity indicated that the strain is related to Massilia niastensis 5516S-1 T (98.3%), M. haematophila CCUG 38318 T (97.9%), M. aerilata 5516S-11 T (97.9%), M. tieshanensis TS3 T (97.6%), and M. varians CCUG 3529 T (97.1%). Colonies grown on Reasoner’s 2A agar at 30°C for 2 days were transparent, white, round, smooth, and glossy. The cells grew at 10–42°C (optimum: 25–37°C) and pH 5–9 (optimum: 5–9) and in 0–2% NaCl (optimum: 0–1%). TSA1 T was able to grow on trypticase soy and nutrient agar, but not on Luria-Bertani or MacConkey agar. The strain was catalase- and oxidasepositive and able to degrade starch and casein, but not carboxymethyl cellulose. The predominant quinone of TSA1 T was Q-8, the major fatty acids were summed feature 3 and C 16:0, and the DNA G+C content was 66.7 mol%. Given these findings, we propose that this strain is a novel species of the genus Massilia. We suggest the name Massilia kyonggiensis sp. nov. (type strain, KACC 17471 T =KEMB 9005-031 T =JCM 19189 T). 相似文献
7.
Antonie van Leeuwenhoek - Three Gram-stain-negative, aerobic, circular, convex, red-colored and rod-shaped bacterial strains, designated BT439T, BT662T and BT683T were obtained from soil collected... 相似文献
9.
Archives of Microbiology - Strain MA2T was isolated from a soil sample from Gijang-gun, Busan in Korea. The strain, a Gram-stain-negative aerobic bacterium, is non-motile, ovoid- or rod-shaped,... 相似文献
10.
A Gram-staining-negative, rod-shaped and red-pigmented bacterial strain, HMD3125 T, was isolated from a solar saltern in Jeungdo, Republic of Korea. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMD3125 T formed a lineage within the genus Pontibacter and was similar to Pontibacter salisaro (96.1%) and P. korlensis (95.3%). The major fatty acids of strain HMD3125 T were summed feature 4 (comprising iso-C 17:1 I and/or anteiso-C 17:1 B; 30.4%), iso-C 15:0 (20.4%) and iso-C 17:0 3OH (17.2%). The polar lipid profile of HMD3125 T consisted of the phosphatidylethanolamine, four unidentified polar lipids, unidentified phospholipid, unidentified aminolipid and unidentified aminophospholipid. Strain HMD3125 T contained MK-7 as the predominant menaquinone and sym-homospermidine as the major polyamine. The DNA G+C content of strain HMD3125 T was 45.6 mol%. Strain HMD3125 T assigned as a novel species in the genus Pontibacter, for which the name Pontibacter jeungdoensis sp. nov. is proposed. The type strain is HMD3125 T (=KCTC 23156 T =CECT 7710 T). 相似文献
11.
A Gram-negative, rod-shaped, non-spore-forming and motile bacterial strain TR7-01(T) was isolated from a compost soil in South Korea and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TR7-01(T) belonged to the genus Hydrogenophaga within the class Betaproteobacteria. Strain TR7-01(T) exhibited 16S rRNA gene sequence similarity values of 95.0-98.3% to members of the genus Hydrogenophaga: Hydrogenophaga bisanensis DSM12412(T) (98.3%), Hydrogenophaga flava DSM 619(T) (97.1%), Hydrogenophaga pseudoflava ATCC 33668(T) (96.8%), Hydrogenophaga intermedia S1(T) (96.4%), Hydrogenophaga atypica BSB 41.8(T) (95.8%), Hydrogenophaga defluvii BSB 9.5(T) (95.7%), Hydrogenophaga palleronii CCUG 20334(T) (95.6%), Hydrogenophaga caeni EMB71(T) (95.4%) and Hydrogenophaga taeniospiralis ATCC 49743(T) (95.0%). Chemotaxonomic data revealed that strain TR7-01(T) possesses ubiquinone Q-8, the G+C content was 69.9 mol%, and the predominant fatty acids were 16 : 1 ω7c/15 : 0 iso 2OH, 18 : 1 ω7c/ω9t/ω12t and C(16:0), all of which corroborated our assignment of the strain to the genus Hydrogenophaga. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain TR7-01(T) represents a distinct species. Based on these data, TR7-01(T) (= KCTC 12203(T) = DSM 18117(T)) should be classified as a novel Hydrogenophaga species, for which the name Hydrogenophaga temperata sp. nov. has been proposed. 相似文献
12.
A non-motile, Gram-stain-negative, yellow pigmented, rod-shaped bacterium, strain HMD3159 T, was isolated from a solar saltern in Korea. The major fatty acids were iso-C 15:0 (26.3%), iso-C 17:0 3OH (12.1%), iso-C 16:0 (12.0%), summed feature 3 (comprising C 16:1
ω7 c and/or C 16:1
ω6 c; 11.0%) and summed feature 9 (iso-C17:1 ω9c and/or 10-methyl C 16:0; 10.0%). The major respiratory quinone was MK-6. The DNA G+C content was 40.9 mol%. The phylogenetic tree based on 16S rRNA
gene sequences showed that strain HMD3159 T formed a lineage within the genus Gramella and closely related to Gramella gaetbulicola (95.5% sequence similarity), Gramella portivictoriae (94.9%), Gramella echinicola (94.6%), and Gramella marina (93.6%). On
the basis of the evidence presented in this study, strain HMD3159 T represents a novel species of the genus Gramella, for which the name Gramella jeungdoensis sp. nov., is proposed. The type strain is HMD3159 T (=KCTC 32123 T =CECT 7683 T). 相似文献
13.
A novel bacterium, designated strain 13-2-B6 T, was isolated from seawater adjacent to Songak Mountain on Jeju Island, South Korea. The novel strain was observed to be Gram-negative, aerobic, rod-shaped and motile with a single polar flagellum. On the basis of 16S rRNA gene sequence similarity, strain 13-2-B6 T was determined to be phylogenetically closely related to the type strain of Antarctobacter heliothermus, currently the sole species of the genus Antarctobacter (family Rhodobacteraceae). Sequence similarity between the 16S rRNA genes of strain 13-2-B6 T and A. heliothermus EL-219 T is 96.9 %. Strain 13-2-B6 T was found to grow optimally at 25–30 °C, pH 7.0–8.0 and 3 % (w/v) NaCl. The predominant isoprenoid quinone in strain 13-2-B6 T was identified as ubiquinone Q-10 and the major fatty acids were identified as C 18:1 ω7c and/or C 18:1 ω6c. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unknown aminolipids, two unknown phospholipids, an unknown glycolipid and an unknown lipid were found to be components of the polar lipid profile. The G + C content of strain 13-2-B6 T was determined to be 62 mol %. On the basis of its phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain 13-2-B6 T is considered to represent a novel species of the genus Antarctobacter, for which the name Antarctobacter jejuensis sp. nov. is proposed. The type strain is 13-2-B6 T (=KCTC 42009 T =JCM 19898 T). 相似文献
14.
Evidence from numerical taxonomic analysis and DNA-DNA hybridization supports the proposal of new species in the genera Actinobacillus and Pasteurella. The following new species are proposed: Actinobacillus rossii sp. nov., from the vaginas of postparturient sows; Actinobacillus seminis sp. nov., nom. rev., associated with epididymitis of sheep; Pasteurella bettii sp. nov., associated with human Bartholin gland abscess and finger infections; Pasteurella lymphangitidis sp. nov. (the BLG group), which causes bovine lymphangitis; Pasteurella mairi sp. nov., which causes abortion in sows; and Pasteurella trehalosi sp. nov., formerly biovar T of Pasteurella haemolytica, which causes septicemia in older lambs. 相似文献
15.
Antonie van Leeuwenhoek - 相似文献
16.
Mycoplasmas isolated from the throats of lions were shown to belong to three serotypes, all of which were serologically distinct from the previously recognized Mycoplasma and Acholeplasma spp. Eight mycoplasma colonies were cloned, including one from a leopard (strain LP), and were examined in detail for morphology, growth, and biochemical characteristics. The strains had the following properties: guanine-plus-cytosine contents of 37 mol% (strain LXT [T = type strain]), 28 mol% (strain LL2T), and 27 mol% (strain 3L2T) and a requirement for sterol. Strain 3L2T metabolized glucose, which was not metabolized by strains LXT and LL2T. Arginine and urea were not hydrolyzed. Strain LX (= NCTC 11724) is the type strain of a new species, Mycoplasma simbae; strain LL2 (= NCTC 11725) is the type strain of a second new species, Mycoplasma leopharyngis; and strain 3L2 (= NCTC 11726) is the type strain of a third new species, Mycoplasma leocaptivus. 相似文献
18.
A Gram-positive strain designated as MSL-14T isolated from a soil sample collected from Bigeum Island, Korea, was subjected to polyphasic taxonomy. The isolate was strictly aerobic. Cells were short rods and motile. Optimum growth temperature and pH was 28 degrees and 7.0, respectively. It was characterized chemotaxonomically as having a cell-wall peptidoglycan type based on LL-2,6-diaminopimelic acid and MK-8(H4) as the predominant menaquinone. The major fatty acids were iso-C16:0, C17:1 omega8c, and C18:1 omega9c. The G+C content was 67.6 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain MSL-14T is affiliated to the genus Nocardioides and formed a distinct lineage within the genus. MSL-14T showed highest sequence similarity to Nocardioides aestuarii JCM 12125T, having a similarity of 96.5%. Based on the 16S rRNA gene sequence divergence and phenotypic characteristics, it is proposed that strain MSL-14T should be classified as representing a novel member of the genus Nocardioides, for which we propose the name Nocardioides tritolerans sp. nov. The type strain is strain MSL-14T (=KCTC 19289T= DSM 19320T). 相似文献
19.
Two Gram-negative, nonmotile, coccobacilli, SW-3T and SW-100T, were isolated from sea water of the Yellow Sea in Korea. Strains SW-3T and SW-100T contained ubiquinone-9 (Q-9) as the predominant respiratory lipoquinone and C18:1 omega9c and C16:0 as the major fatty acids. The DNA G+C contents of strains SW-3T and SW-100T were 44.1 mol% and 41.9 mol%, respectively. A neighbor-joining tree based on 16S rRNA gene sequences showed that the two isolates fell within the evolutionary radiation enclosed by the genus Acinetobacter. Strains SW-3T and SW-100T exhibited a 16S rRNA gene similarity value of 95.7% and a mean DNA-DNA relatedness level of 9.2%. Strain SW-3T exhibited 16S rRNA gene sequence similarity levels of 93.5-96.9% to the validly described Acinetobacter species and fifteen Acinetobacter genomic species. Strain SW-100T exhibited 16S rRNA gene sequence similarity levels of less than 97.0% to the other Acinetobacter species except Acinetobacter towneri DSM 14962T (98.0% similarity). Strains SW-3T and SW-100T exhibited mean levels of DNA-DNA relatedness of 7.3-16.7% to the type strains of some phylogenetically related Acinetobacter species. On the basis of phenotypic, phylogenetic, and genetic data, strains SW-3T and SW-100T were classified in the genus Acinetobacter as two distinct novel species, for which the names Acinetobacter marinus sp. nov. (type strain SW-3T=KCTC 12259T=DSM 16312T) and Acinetobacter seohaensis sp. nov. (type strain SW-100T=KCTC 12260T=DSM 16313T) are proposed, respectively. 相似文献
20.
Three strains, YP416 T, YP421 T, and Y422, were isolated from soil samples in Pocheon City, Gyeonggi province, South Korea. The strains belong to two novel yeast species in the genus Mrakia. Molecular phylogenetic analysis showed that the strain YP416 T was closely related to Mrakia niccombsii. Still, it differed by 9 nucleotide substitutions with no gap (1.51%) in the D1/D2 domain of the LSU rRNA gene and 14 nucleotide substitutions with 7 gaps (2.36%) in the ITS region. The strain YP421 T differed from the type strain of the most closely related species, Mrakia aquatica, by 5 nucleotide substitutions with no gap (0.81%) in the D1/D2 domain of the LSU rRNA gene and 9 nucleotide substitutions with one gap (1.43%) in the ITS region. The names Mrakia terrae sp. nov. and Mrakia soli sp. nov. are proposed, with type strains YP416 T (KCTC 27886 T) and YP421 T (KCTC 27890 T), respectively. MycoBank numbers of the strains YP416 T and YP421 T are MB 836844 and MB 836847, respectively. 相似文献
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