共查询到20条相似文献,搜索用时 15 毫秒
1.
Min Fei Na Li Yuguan Ze Jie Liu Xiaolan Gong Yanmei Duan Xiaoyang Zhao Han Wang Fashui Hong 《Biological trace element research》2011,139(1):72-80
In order to study the mechanisms underlying the effects of lanthanoid (Ln) on the liver, ICR mice were injected with LaCl3, CeCl3, and NdCl3 at a dose of 20 mg/kg BW into the abdominal cavity daily for 14 days. We then examined oxidative stress-mediated responses
in the liver. The increase of lipid peroxide in the liver produced by Ln suggested an oxidative attack that was activated
by a reduction of antioxidative defense mechanisms as measured by analyzing the activities of superoxide dismutase, catalase,
and ascorbate peroxidase, as well as antioxidant levels such as glutathione and ascorbic acid, which were greatest in Ce3+ treatment, medium in Nd3+, and least in La3+. Our results also implied that the oxidative stress in the liver caused by Ln likely is Ce3+ > Nd3+ >La3+, but the mechanisms need to be further studied in future. 相似文献
2.
Lentivirus(LV)-mediated small interfering RNA (siRNA) targeting tumor necrosis factor-α (TNF-α) was locally administered into
the air pouch of mice to inhibit inflammation induced by titanium alloy particles. The lentiviral vector expressed green fluorescent
protein (GFP) as a reporter gene. Down-regulation of TNF-α in pouch area was confirmed by real-time PCR and ELISA, resulting
in significantly decreased local inflammatory responses (P < 0.01). This approach was proven safe by localized GFP fluorescence and invariant TNF-α expression in peripheral blood,
liver, spleen, kidney, lung and brain of mouse. In conclusion, locally administered siRNA provides an effective and safe method
for inhibiting particle-induced inflammation. 相似文献
3.
Characterization and expression analysis of KAP7.1, KAP8.2 gene in Liaoning new-breeding cashmere goat hair follicle 总被引:2,自引:0,他引:2
Keratin-associated protein is one of the major structural proteins of the hair, whose content in hair has important effect
on the quality of cashmere. In order to study the relationship between HGTKAP gene expression and cashmere fineness, the quantitative
real-time RT–PCR (qRT–PCR) was firstly used to detect the levels of KAP7.1, KAP8.2 gene expression in the primary and secondary
hair follicles; semi-quantitative RT–PCR was used to detect whether KAP7.1, KAP8.2 gene are expressed in heart, liver, spleen,
lung, kidney tissues; and in situ hybridization(ISH) to detect KAP7.1 gene expression location. qRT–PCR result showed that
the expression of both KAP7.1 and KAP8.2 gene in the secondary hair follicles are significantly higher than that in the primary
follicles, relative quantitative analysis obtained that KAP7.1 was 2.28 times, while KAP8.2 was 2.71 times. Semi-quantitative
RT–PCR results revealed that KAP 7.1 and KAP8.2 mRNA were not detected in the heart, liver, spleen, lung and kidney tissues,
demonstrating that KAP7.1 and KAP8.2 were specially expressed in hair follicles, participating in hair formation. Moreover,
KAP7.1 gene has a strong expression in the cortical layer, inner root sheath of the primary follicles and the cortical layer,
inner root sheath and hair matrix of the secondary hair follicles by ISH analysis. Taken together, the evidence presented
here indicated that in the formation of cashmere and wool, differential expression of these two genes in the primary and secondary
hair follicles may have an important role in regulating the fiber diameter. 相似文献
4.
Xiangli Li Blair U Bradford Frederick Dalldorf Sanna M Goyert Stephen A Stimpson Ronald G Thurman Sergei S Makarov 《Arthritis research & therapy》2004,6(3):R273
Bacterial infections play an important role in the multifactorial etiology of rheumatoid arthritis. The arthropathic properties
of Gram-positive bacteria have been associated with peptidoglycan–polysaccharide complexes (PG-PS), which are major structural
components of bacterial cell walls. There is little agreement as to the identity of cellular receptors that mediate innate
immune responses to PG-PS. A glycosylphosphatidylinositol-linked cell surface protein, CD14, the lipopolysaccharide receptor,
has been proposed as a PG-PS receptor, but contradictory data have been reported. Here, we examined the inflammatory and pathogenic
responses to PG-PS in CD14 knockout mice in order to examine the role for CD14 in PG-PS-induced signaling. We found that PG-PS-induced
responses in vitro, including transient increase in intracellular calcium, activation of nuclear factor-κB, and secretion of the cytokines tumor
necrosis factor-α and interleukin-6, were all strongly inhibited in CD14 knockout macrophages. In vivo, the incidence and severity of PG-PS induced acute polyarthritis were significantly reduced in CD14 knockout mice as compared
with their wild-type counterparts. Consistent with these findings, CD14 knockout mice had significantly inhibited inflammatory
cell infiltration and synovial hyperplasia, and reduced expression of inflammatory cytokines in PG-PS arthritic joints. These
results support an essential role for CD14 in the innate immune responses to PG-PS and indicate an important role for CD14
in PG-PS induced arthropathy. 相似文献
5.
Shashi Sharma Paban Kumar Dash S. R. Santhosh Jyoti Shukla Manmohan Parida P. V. Lakshmana Rao 《Molecular biotechnology》2010,45(1):49-55
Chikungunya is one of the most important emerging arboviral infections of public health significance. Due to lack of a licensed
vaccine, rapid diagnosis plays an important role in early management of patients. In this study, a QC-RT–PCR assay was developed
to quantify Chikungunya virus (CHIKV) RNA by targeting the conserved region of E1 gene. A competitor molecule containing an
internal insertion was generated, which provided a stringent control of the quantification process. The introduction of 10-fold
serially diluted competitor in each reaction was further used to determine sensitivity. The applicability of this assay for
quantification of CHIKV RNA was evaluated with human clinical samples, and the results were compared with real-time quantitative
RT–PCR. The sensitivity of this assay was estimated to be 100 RNA copies per reaction with a dynamic detection range of 102 to 1010 copies. Specificity was confirmed using closely related alpha and flaviviruses. The comparison of QC-RT–PCR result with real-time
RT–PCR revealed 100% concordance for the detection of CHIKV in clinical samples. These findings demonstrated that the reported
assay is convenient, sensitive and accurate method and has the potential usefulness for clinical diagnosis due to simultaneous
detection and quantification of CHIKV in acute-phase serum samples. 相似文献
6.
Ming Jun Deng Xi Zhi Xiao Yan Ming Zhang Xin Hai Wu Lai Hua Zhu Xue Qian Xin Dong Lai Wu 《Molecular biology reports》2011,38(3):1941-1948
With an aim at detecting the ultra-low concentration of avian influenza virus (AIV), a highly sensitive hybrid assay based
on immunology and polymerase chain reaction was developed. The TopYield microtiter plates were coated with ten-fold serial
dilutions of H5N1 subtype AIV ranging from 10 EID50 ml−1~10−4 EID50 ml−1,which was recognized by mouse anti-AIV H5 monoclonal antibody (MAb) that was directly linked with reporter DNA using a heterobifunctional
cross-linker. After extensive washing, the reporter DNA including a BamH I-restriction site was released by a specific enzymatic restriction, then transferred to PCR tubes, amplified, and used
as the signal for detection of AIV. Under the optimized condition, MAb-based immuno-PCR (IPCR) method could measure 100 μl
of AIV H5N1 with 10−4 EID50 ml−1.To evaluate the sensitivity of IPCR, the same concentration and volume of AIV H5N1 were detected by conventional RT–PCR and
sandwich ELISA. The results showed that IPCR had an approximately 1,000-fold improvement over the conventional ELISA, and
a 100-fold enhancement compared with RT–PCR in detection sensitivity. To further evaluate the specificity of IPCR for AIV
H5 subtype, the tracheal swab specimens, taken from chickens which were infected with H9N2, and the allantoic fluid from eggs
inoculated by AIV H3N2, H7N1, H9N2, were detected by IPCR. To mimic clinical samples, pharyngeal–tracheal swab specimens were
collected from healthy chickens and spiked with H5N1, H5N2, H5N3 for analysis by immuno-PCR. The results demonstrated that
IPCR was a highly sensitive and specific assay for AIV H5, and could be applied to clinical detection for low amount of AIV
H5 subtype. This MAb-based immuno-PCR method provided a platform capable of mass screening of clinical samples for AIV H5
subtype and could serve as a model for other immuno-PCR assays. 相似文献
7.
8.
Haiquan Zhao Zhe Cheng Renping Hu Jie Chen Mengmeng Hong Min Zhou Xiaolan Gong Ling Wang Fashui Hong 《Biological trace element research》2011,142(2):174-189
The organ toxicity of lanthanides (Ln) on organisms had been recognized, but very little is known about the oxidative injury
of brain caused by Ln. In order to study the mechanisms underlying the effects of Ln on the brain, ICR mice were injected
with a single 20 mg/kg body weight dose of LaCl3, CeCl3, and NdCl3 into the abdominal cavity daily for 14 days. We then examined the coefficient of the brain, the brain pathological changes
and oxidative stress-mediated responses, and the accumulation of Ln and levels of neurochemicals in the brain. The results
showed that CeCl3 and NdCl3 could induce some neurons to turn inflammatory cells and slight edema but did not observe the brain pathological changes
from LaCl3-treated group. The concentrations of La, Ce, and Nd in the brain were significantly different and ranked in the order of
Ce, Nd, and La. The injury of the brain and oxidative stress occurred as Ln appeared to trigger a cascade of reactions such
as lipid peroxidation, the decreases of the total antioxidation capacity and activities of antioxidative enzymes, the excessive
release of nitric oxide, the increase of glutamic acid, and the downregulated level of acetylcholinesterase activities. Furthermore,
both Ce3+ and Nd3+ exhibited higher oxidative stress and toxicity on brain than La3+, and Ce3+ caused more severe brain injuries and oxidative stress than Nd3+, implying that the differences in the brain injuries caused by Ln might be related to the number of 4f electrons of Ln. 相似文献
9.
Cheng J Li N Cai J Cheng Z Hu R Zhang Q Wan F Sun Q Gui S Sang X Wang L Hong F 《Biological trace element research》2012,145(3):361-368
Due to increasing applications of lanthanides (Ln) in industry and daily life, numerous studies confirmed that Ln exposure may result in organ damages in mice and rats, while very few studies focused on several organs damages simultaneously. In order to compare the toxicity of Ln on organs, mice were exposed to LaCl(3), CeCl(3), and NdCl(3) of a dose of 20 mg/kg body weight for consecutive 60 days, respectively, then histopathological changes of liver, kidney, and heart, and their function were investigated. The results showed that long-term exposure to Ln caused cell necrosis and basophilia of liver, ambiguity of renal tubule architecture, congestion of blood vessel and capillary of kidney, and heart hemorrhage. The histopathological changes of liver, kidney, and heart in mice caused by Ce(3+) was most severe; the effect by Nd(3+) was slighter than Ce(3+) but more severe than La(3+). The assay of serum biochemical parameters suggested that Ln exposure severely impaired the functions of liver, kidney, and myocardium in mice. These findings suggested that long-term exposure to Ln resulted in histopathological changes of liver, kidney, and heart, and their function damages. Therefore, we thought that long-term application of the products containing Ln on human should be cautious. 相似文献
10.
11.
The knowledge of the impact of elevated ground-level O3 below ground the agro-ecosystem is limited. A field experiment in China Ozone Free-Air Concentration Enrichment (FACE-O3) facility on a rice–wheat rotation system was carried out to investigate responses of anoxygenic phototrophic purple bacteria
(AnPPB) to elevated ground-level O3. AnPPB community structures and sizes in paddy soil were monitored by molecular approaches including PCR–DGGE and real-time
quantitative PCR based upon the pufM gene on three typical rice growth stages. Repetitive sequence-based PCR (rep-PCR) in combination with culture-reliant method
was conducted to reveal changes in genotypic diversity. Elevated ground-level O3 statistically reduce AnPPB abundance and percentage in total bacterial community in flooded rice soil via decreasing their
genotypic diversity and metabolic versatility. Concomitantly, their community composition changed after rice anthesis stage
under elevated ground-level O3. Our results from AnPPB potential responses imply that continuously elevated ground-level O3 in the future would eventually harm the health of paddy ecosystem through negative effect on soil microorganisms. 相似文献
12.
13.
B. Cukrowska I. Rosiak E. Klewicka I. Motyl M. Schwarzer Z. Libudzisz H. Kozakova 《Folia microbiologica》2010,55(3):277-280
Heat-inactivated Lactobacillus casei LOCK 0900, L. casei LOCK 0908 and Lactobacillus paracasei LOCK 0919 strains, applied to blood cell cultures obtained from children with atopic dermatitis induced production of anti-allergic
TH1 cytokines (interleukin-12, interleukin-18, interferon-γ, tumor necrosis factor-α) and regulatory transforming growth factor-β1), but did not stimulate pro-allergic interleukin-5. The lactobacilli-mixture remarkably enhanced the TH1 response compared to single strains. This synergistic effect was not observed for transforming growth factor-β1. In contrast, the amount of interleukin-10 was found to be considerably lower when cells were stimulated with lactobacilli-mixture
compared to single strains. The mixture of Lactobacillus strains represents a probiotic bacterial preparation modulating in vitro cytokine profile of allergic children towards anti-allergic TH1 response. 相似文献
14.
Sung-Hwa Sohn Sun Kwang Kim Eunjung Ko Youngseop Lee Hwan-Suck Chung Hyojung Lee Hyunseong Kim Deok-Sang Hwang Sangsoo Nam Hyunsu Bae 《Cellular and molecular neurobiology》2010,30(4):631-640
Previously, we demonstrated that electoracupuncture (EA) suppressed allergic reactions in DNP-KLH immunized mice. In this
study, the mechanisms by which EA induces immunomodulation in the immunized mice were evaluated by genome-wide microarray
analysis. The anti-allergic effects of EA in DNP-KLH immunized mice were confirmed by analyzing antigen specific IgE using
ELISA. Microarray analysis, followed by real time RT–PCR validation, revealed that Th1 and Th17 cytokine-, opioid peptide-,
and anti-apoptosis-related genes were up-regulated upon treatment with EA. In addition, significant decreases in Th2 cytokine-,
MAPK signaling pathway-, and apoptosis-related genes were observed following EA treatment. 相似文献
15.
Hye-Jeong Cho Sungbum Kim Sung-Eun Kwak Tae-Cheon Kang Hee-Sung Kim Hyung-Joo Kwon Yoon-Won Kim Yong-Sun Kim Eun-Kyung Choi Moon Jung Song 《Molecules and cells》2009,27(1):105-111
Gammaherpesvirus infection of the central nervous system (CNS) has been linked to various neurological diseases, including
meningitis, encephalitis, and multiple sclerosis. However, little is known about the interactions between the virus and the
CNS in vitro or in vivo. Murine gammaherpesvirus 68 (MHV-68 or γHV-68) is genetically related and biologically similar to human gammaherpesviruses, thereby providing a tractable animal model
system in which to study both viral pathogenesis and replication. In the present study, we show the successful infection of
cultured neuronal cells, microglia, and astrocytes with MHV-68 to various extents. Upon intracerebroventricular injection
of a recombinant virus (MHV-68/LacZ) into 4–5-week-old and 9–10-week-old mice, the 4–5-week-old mice displayed high mortality
within 5–7 days, while the majority of the 9–10-week-old mice survived until the end of the experimental period. Until a peak
at 3–4 days post-infection, viral DNA replication and gene expression were similar in the brains of both mouse groups, but
only the 9–10-week-old mice were able to subdue viral DNA replication and gene expression after 5 days post-infection. Pro-inflammatory
cytokine mRNAs of tumor necrosis factor-α, interleukin 1β, and interleukin 6 were highly induced in the brains of the 4–5-week-old
mice, suggesting their possible contributions as neurotoxic factors in the agedependent control of MHV-68 replication of the
CNS.
These authors contributed equally to this work. 相似文献
16.
Calcium fructoborate is a boron-based nutritional supplement. Its chemical structure is similar to one of the natural forms
of boron such as bis-manitol, bis-sorbitol, bis-fructose, and bis-sucrose borate complexes found in edible plants. In vitro studies revealed that calcium fructoborate is a superoxide ion
scavenger and anti-inflammatory agent. It may influence macrophage production of inflammatory mediators, can be beneficial
for the suppression of cytokine production, and inhibits progression of endotoxin-associated diseases, as well as the boric
acid and other boron sources. The mechanisms by which calcium fructoborate exerts its beneficial anti-inflammatory effects
are not entirely clear, but some of its molecular biological in vitro activities are understood: inhibition of the superoxide
within the cell; inhibition of the interleukin-1β, interleukin-6, and nitric oxide release in the culture media; and increase
of the tumor necrosis factor-α production. Also, calcium fructoborate has no effects on lipopolysaccharide-induced cyclooxygenase-2
protein express. The studies on animals and humans with a dose range of 1–7 mg calcium fructoborate (0.025–0.175 mg elemental
boron)/kg body weight/day exhibited a good anti-inflammatory activity, and it also seemed to have negligible adverse effect
on humans. 相似文献
17.
Objective
Recently, salusin-β has been reported to have pro-atherosclerotic effects, but salusin-α has anti-atherosclerotic effects. Our previous study has shown that salusin-β but not salusin-α promotes vascular inflammation in apoE-deficient mice. However, the underlying mechanism remains unknown. In this study, we observed the effect of salusins on inflammatory responses and the MAPK-NF-κB signaling pathway in human umbilical vein endothelial cells (HUVECs).Methods and Results
HUVECs were incubated with different concentrations of salusin-α and salusin-β. The levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were determined using enzyme-linked immunosorbent assay (ELISA). The mRNA expressions of vascular cell adhesion molecule-1 (VCAM-1) and monocyte chemoattractant protein-1 (MCP-1) were quantified using quantitative real-time polymerase chain reaction (PCR). The protein expressions of VCAM-1, MCP-1, I-κBα, NF-κB, p-JNK and p-p38 MAPK were measured using western blotting analysis. Our results showed that in HUVECs, salusin-β could up-regulate the levels of IL-6, TNF-α, VCAM-1 and MCP-1, promote I-κBα degradation and NF-κB activation, and increase the phosphorylation of JNK and p38 MAPK. These effects could be inhibited by p38 MAPK inhibitor SB203580 and/or JNK inhibitor SP600125. In contrast, salusin-α could selectively decrease VCAM-1 protein, but did not show any effect on the expressions of VCAM-1 mRNA, TNF-α, IL-6, MCP-1, I-κBα, NF-κB, p-JNK or p-p38 MAPK.Conclusion
Salusin-β was able to promote inflammatory responses in HUVECs via the p38 MAPK-NF-κB and JNK-NF-κB pathways. In contrast, salusin-α failed to show any significant effects on the inflammatory responses in HUVECs. These results provide further insight into the mechanisms behind salusins in vascular inflammation and offer a potential target for the prevention and treatment of atherosclerosis. 相似文献18.
19.
Ting-Chun Weng Chien-Chang Shen Yung-Tsung Chiu Yun-Lian Lin Cheng-Deng Kuo Yi-Tsau Huang 《Journal of biomedical science》2009,16(1):78-13
Activation of hepatic stellate cells (HSCs) plays a crucial role in liver fibrogenesis. armepavine (Arm, C19H23O3N), an active compound from Nelumbo nucifera, has been shown to exert immunosuppressive effects on T lymphocytes and on lupus nephritic mice. The aim of this study was
to investigate whether Arm could exert anti-hepatic fibrogenic effects in vitro and in vivo. A cell line of rat HSCs (HSC-T6) was stimulated with tumor necrosis factor-α (TNF-α) or lipopolysaccharide (LPS) to evaluate
the inhibitory effects of Arm. An in vivo therapeutic study was conducted in bile duct-ligated (BDL) rats. BDL rats were given Arm (3 or 10 mg/kg) by gavage twice
daily for 3 weeks starting from the onset of BDL. Liver sections were taken for fibrosis scoring, immuno-fluorescence staining
and quantitative real-time mRNA measurements. In vitro, Arm (1-10 μM) concentration-dependently attenuated TNF-α- and LPS-stimulated α-SMA protein expression and AP-1 activation
by HSC-T6 cells without adverse cytotoxicity. Arm also suppressed TNF-α-induced collagen collagen deposition, NFκB activation
and MAPK (p38, ERK1/2, and JNK) phosphorylations. In vivo, Arm treatment significantly reduced plasma AST and ALT levels, hepatic α-SMA expression and collagen contents, and fibrosis
scores of BDL rats as compared with vehicle treatment. Moreover, Arm attenuated the mRNA expression levels of col 1α2, TGF-β1, TIMP-1, ICAM-1, iNOS, and IL-6 genes, but up-regulated metallothionein genes. Our study results showed that Arm exerted both in vitro and in vivo antifibrotic effects in rats, possibly through anti-NF-κB activation pathways. 相似文献