共查询到20条相似文献,搜索用时 15 毫秒
1.
Morgenstern B Prohaska SJ Pöhler D Stadler PF 《Algorithms for molecular biology : AMB》2006,1(1):6-12
Background
Automated software tools for multiple alignment often fail to produce biologically meaningful results. In such situations, expert knowledge can help to improve the quality of alignments. 相似文献2.
Background
There have been many algorithms and software programs implemented for the inference of multiple sequence alignments of protein and DNA sequences. The "true" alignment is usually unknown due to the incomplete knowledge of the evolutionary history of the sequences, making it difficult to gauge the relative accuracy of the programs. 相似文献3.
Background
For effective exposition of biological information, especially with regard to analysis of large-scale data types, researchers need immediate access to multiple categorical knowledge bases and need summary information presented to them on collections of genes, as opposed to the typical one gene at a time. 相似文献4.
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Stéphane Descorps-Declère Danielle Ziébelin François Rechenmann Alain Viari 《BMC bioinformatics》2006,7(1):450-13
Background
Genome annotation can be viewed as an incremental, cooperative, data-driven, knowledge-based process that involves multiple methods to predict gene locations and structures. This process might have to be executed more than once and might be subjected to several revisions as the biological (new data) or methodological (new methods) knowledge evolves. In this context, although a lot of annotation platforms already exist, there is still a strong need for computer systems which take in charge, not only the primary annotation, but also the update and advance of the associated knowledge. In this paper, we propose to adopt a blackboard architecture for designing such a system 相似文献7.
8.
Steven Weyers Hans Verstraelen Jan Gerris Stan Monstrey Guido dos Santos Lopes Santiago Bart Saerens Ellen De Backer Geert Claeys Mario Vaneechoutte Rita Verhelst 《BMC microbiology》2009,9(1):102-10
Background
The microflora of the penile skin-lined neovagina in male-to-female transsexuals is a recently created microbial niche which thus far has been characterized only to a very limited extent. Yet the knowledge of this microflora can be considered as essential to the follow-up of transsexual women. The primary objective of this study was to map the neo-vaginal microflora in a group of 50 transsexual women for whom a neovagina was constructed by means of the inverted penile skin flap technique. Secondary objectives were to describe possible correlations of this microflora with multiple patients' characteristics, such as sexual orientation, the incidence of vaginal irritation and malodorous vaginal discharge. 相似文献9.
Background
Analysis of any newly sequenced bacterial genome starts with the identification of protein-coding genes. Despite the accumulation of multiple complete genome sequences, which provide useful comparisons with close relatives among other organisms during the annotation process, accurate gene prediction remains quite difficult. A major reason for this situation is that genes are tightly packed in prokaryotes, resulting in frequent overlap. Thus, detection of translation initiation sites and/or selection of the correct coding regions remain difficult unless appropriate biological knowledge (about the structure of a gene) is imbedded in the approach. 相似文献10.
Background
Meta-analysis methods exist for combining multiple microarray datasets. However, there are a wide range of issues associated with microarray meta-analysis and a limited ability to compare the performance of different meta-analysis methods. 相似文献11.
Background
Previous studies have demonstrated poor knowledge of stroke among patients with established risk factors. This study aims to assess the baseline knowledge, among patients with increased risk for stroke in Oman, of warning symptoms of stroke, impending risk factors, treatment, and sources of information. 相似文献12.
Background
Analysis of variance is a powerful approach to identify differentially expressed genes in a complex experimental design for microarray and macroarray data. The advantage of the anova model is the possibility to evaluate multiple sources of variation in an experiment. 相似文献13.
Background
The simultaneous production of various recombinant proteins in every cell of a culture is often needed for the production of virus-like particles (VLP) or vectors for gene therapy. A common approach for such a purpose is the coinfection of insect cell cultures with different recombinant baculoviruses, each containing one or more recombinant genes. However, scarce information exists regarding kinetics during multiple infections, and to our knowledge, no studies are available on the behavior of the different populations that arise during coinfections. Such information is useful for designing infection strategies that maximize VLP or vector yield. In this work, kinetics of cell populations expressing rotavirus GFPVP2 (infected with bacGFPVP2), VP6 (infected with bacVP6), or both proteins simultaneously (coinfected with both baculoviruses) were followed by flow cytometry. 相似文献14.
Holger Wagner Burkhard Morgenstern Andreas Dress 《Algorithms for molecular biology : AMB》2008,3(1):15
Background
Sequence-based phylogeny reconstruction is a fundamental task in Bioinformatics. Practically all methods for phylogeny reconstruction are based on multiple alignments. The quality and stability of the underlying alignments is therefore crucial for phylogenetic analysis. 相似文献15.
Nelson AE DeVellis RF Renner JB Schwartz TA Conaghan PG Kraus VB Jordan JM 《Arthritis research & therapy》2011,13(5):R176
Introduction
Although osteoarthritis (OA) commonly involves multiple joints, no widely accepted method for quantifying whole-body OA burden exists. Therefore, our aim was to apply factor analytic methods to radiographic OA (rOA) grades across multiple joint sites, representing both presence and severity, to quantify the burden of rOA. 相似文献16.
Prachi Kothiyal Stephanie Cox Jonathan Ebert Ammar Husami Margaret A Kenna John H Greinwald Bruce J Aronow Heidi L Rehm 《BMC biotechnology》2010,10(1):10
Background
Despite current knowledge of mutations in 45 genes that can cause nonsyndromic sensorineural hearing loss (SNHL), no unified clinical test has been developed that can comprehensively detect mutations in multiple genes. We therefore designed Affymetrix resequencing microarrays capable of resequencing 13 genes mutated in SNHL (GJB2, GJB6, CDH23, KCNE1, KCNQ1, MYO7A, OTOF, PDS, MYO6, SLC26A5, TMIE, TMPRSS3, USH1C). We present results from hearing loss arrays developed in two different research facilities and highlight some of the approaches we adopted to enhance the applicability of resequencing arrays in a clinical setting. 相似文献17.
Background
Recent advances in molecular biology techniques provide an opportunity for developing detailed mathematical models of biological processes. An iterative scheme is introduced for model identification using available system knowledge and experimental measurements. 相似文献18.
Zhijun Wang Li Xiang Junjie Shao Alicja Węgrzyn Grzegorz Węgrzyn 《Microbial cell factories》2006,5(1):34-18
Background
Although understanding of physiological interactions between plasmid DNA and its host is important for vector design and host optimization in many biotechnological applications, to our knowledge, global studies on plasmid-host interactions have not been performed to date even for well-characterized plasmids. 相似文献19.
Douglas S Goodin 《BMC neurology》2010,10(1):101
Background
MS-pathogenesis is known to involve both multiple environmental events, and several independent genetic risk-factors. 相似文献20.
Bob?JA?Schijvenaars Barend?Mons Marc?Weeber Martijn?J?Schuemie Erik?M?van Mulligen Hester?M?Wain Jan?A?Kors