共查询到20条相似文献,搜索用时 15 毫秒
1.
Unrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres
Faten Zaibak Paul Bello Jennifer Kozlovski Duncan Crombie Haozhi Ang Mirella Dottori Robert Williamson 《BMC biotechnology》2009,9(1):101-16
Background
Human umbilical cord blood-derived unrestricted somatic stem cells (USSCs), which are capable of multilineage differentiation, are currently under investigation for a number of therapeutic applications. A major obstacle to their clinical use is the fact that in vitro expansion is still dependent upon fetal calf serum, which could be a source of pathogens. In this study, we investigate the capacity of three different stem cell culture media to support USSCs in serum-free conditions; HEScGRO™, PSM and USSC growth mediumACF. Our findings demonstrate that USSCs do not grow in HEScGRO™ or PSM, but we were able to isolate, proliferate and maintain multipotency of three USSC lines in USSC growth mediumACF. 相似文献2.
Patricia Marqués-Gallego Hans den Dulk Claude Backendorf Jaap Brouwer Jan Reedijk Julian F Burke 《BMC biotechnology》2010,10(1):43
Background
The CloneSelect™ Imager system is an image-based visualisation system for cell growth assessment. Traditionally cell proliferation is measured with the colorimetric MTT assay. 相似文献3.
JChristopher Anderson John E Dueber Mariana Leguia Gabriel C Wu Jonathan A Goler Adam P Arkin Jay D Keasling 《Journal of biological engineering》2010,4(1):1
Background
Standard biological parts, such as BioBricks™ parts, provide the foundation for a new engineering discipline that enables the design and construction of synthetic biological systems with a variety of applications in bioenergy, new materials, therapeutics, and environmental remediation. Although the original BioBricks™ assembly standard has found widespread use, it has several shortcomings that limit its range of potential applications. In particular, the system is not suitable for the construction of protein fusions due to an unfavorable scar sequence that encodes an in-frame stop codon. 相似文献4.
Background
Mascot™ is a commonly used protein identification program for MS as well as for tandem MS data. When analyzing huge shotgun proteomics datasets with Mascot™'s native tools, limits of computing resources are easily reached. Up to now no application has been available as open source that is capable of converting the full content of Mascot™ result files from the original MIME format into a database-compatible tabular format, allowing direct import into database management systems and efficient handling of huge datasets analyzed by Mascot™. 相似文献5.
6.
Wenling E Chang Keri Sarver Brandon W Higgs Timothy D Read Nichole ME Nolan Carol E Chapman Kimberly A Bishop-Lilly Shanmuga Sozhamannan 《BMC bioinformatics》2011,12(1):109
Background
OmniLog™ phenotype microarrays (PMs) have the capability to measure and compare the growth responses of biological samples upon exposure to hundreds of growth conditions such as different metabolites and antibiotics over a time course of hours to days. In order to manage the large amount of data produced from the OmniLog™ instrument, PheMaDB (Phenotype Microarray DataBase), a web-based relational database, was designed. PheMaDB enables efficient storage, retrieval and rapid analysis of the OmniLog™ PM data. 相似文献7.
Shuyu Wu Eirini Chouliara Lars Bogø Jensen Anders Dalsgaard 《Acta veterinaria Scandinavica》2008,50(1):38
Background
Screening and enumeration of antimicrobial resistant Escherichia coli directly from samples is needed to identify emerging resistant clones and obtain quantitative data for risk assessment. Aim of this study was to evaluate the performance of 3M™ Petrifilm™ Select E. coli Count Plate (SEC plate) supplemented with antimicrobials to discriminate antimicrobial-resistant and non-resistant E. coli. 相似文献8.
Molla Kazemiha V Azari S Amanzadeh A Bonakdar S Shojaei Moghadam M Habibi Anbouhi M Maleki S Ahmadi N Mousavi T Shokrgozar MA 《Cytotechnology》2011,63(6):609-620
Mycoplasma contamination is a deleterious event for cell culture laboratories. Plasmocin™ is used to prevent and eradicate
mycoplasma infections from cell. In this study, 80 different mammalian cell lines from various sources; human, monkey, mice,
hamster and rat were used to study and evaluate plasmocin™ efficiency and compare it to commonly used antibiotics such as
BM-cyclin, ciprofloxacin and mycoplasma removal agent (MRA). It was shown that mycoplasma infections were eradicated by plasmocin™,
BM-cyclin, ciprofloxacin and MRA in 65%, 66.25%, 20%, and 31.25%, respectively, of infected cell cultures. However, re-infection
with mycoplasmas after the period of 4 months occurred in 10–80% of the studied cell lines. Cell cytotoxicity and culture
death was observed in 25, 17.5 and 10% of the treated cells, for plasmocin™, BM-cyclin and MRA, respectively. In this study,
Plasmocin™ showed strong ability to eradicate mollicutes from our cell lines with minimal percentage of regrowth. However,
due to its high cell cytotoxicity it should be used with caution especially when dealing with expensive or hard-to-obtain
cell lines. Amongst the antibiotics tested, BM-cyclin was shown to remove mycoplasma with the highest efficiency. 相似文献
9.
Yi-Ting Wu Che Yi Lin Ming-Yuan Tsai Yi-Hua Chen Yu-Fen Lu Chang-Jen Huang Chao-Min Cheng Sheng-Ping L Hwang 《Journal of biomedical science》2011,18(1):70
Background
β-Lapachone has antitumor and wound healing-promoting activities. To address the potential influences of various chemicals on heart development of zebrafish embryos, we previously treated zebrafish embryos with chemicals from a Sigma LOPAC1280™ library and found several chemicals including β-lapachone that affected heart morphogenesis. In this study, we further evaluated the effects of β-lapachone on zebrafish embryonic heart development. 相似文献10.
Monika Witusik Sylwester Piaskowski Krystyna Hulas-Bigoszewska Magdalena Zakrzewska Sylwia M Gresner S Ausim Azizi Barbara Krynska Pawel P Liberski Piotr Rieske 《BMC biotechnology》2008,8(1):56
Background
Although extensive research has been performed to control differentiation of neural stem cells – still, the response of those cells to diverse cell culture conditions often appears to be random and difficult to predict. To this end, we strived to obtain stabilized protocol of NHA cells differentiation – allowing for an increase in percentage yield of neuronal cells. 相似文献11.
Leticia Labriola Maria G Peters Karin Krogh Iván Stigliano Letícia F Terra Cecilia Buchanan Marcel CC Machado Elisa Bal de Kier Joffé Lydia Puricelli Mari C Sogayar 《BMC cell biology》2009,10(1):49-16
Background
The in vitro culture of insulinomas provides an attractive tool to study cell proliferation and insulin synthesis and secretion. However, only a few human beta cell lines have been described, with long-term passage resulting in loss of insulin secretion. Therefore, we set out to establish and characterize human insulin-releasing cell lines. 相似文献12.
Monica Andersson Malin Warolén Joakim Nilsson Martin Selander Catharina Sterky Katrin Bergdahl Christina Sörving Stephen R James Magnus Doverskog 《BMC cell biology》2007,8(1):6
Background
Recombinant adenovirus vectors and transfection agents comprising cationic lipids are widely used as gene delivery vehicles for functional expression in cultured cells. Consequently, these tools are utilized to investigate the effects of functional over-expression of proteins on insulin mediated events. However, we have previously reported that cationic lipid reagents cause a state of insulin unresponsiveness in cell cultures. In addition, we have found that cultured cells often do not respond to insulin stimulation following adenovirus treatment. Infection with adenovirus compromises vital functions of the host cell leading to the activation of protein kinases central to insulin signalling, such as protein kinase B/Akt. Therefore, we investigated the effect of adenovirus infection on insulin unresponsiveness by means of Akt activation in cultured cells. Moreover, we investigated the use of baculovirus as a heterologous viral gene delivery vehicle to circumvent these phenomena. Since the finding that baculovirus can efficiently transduce mammalian cells, the applications of this viral system in gene delivery has greatly expanded and one advantage is the virtual absence of cytotoxicity in mammalian cells. 相似文献13.
Eudes de Crecy Stefan Jaronski Benjamin Lyons Thomas J Lyons Nemat O Keyhani 《BMC biotechnology》2009,9(1):74
Background
Filamentous fungi are the most widely used eukaryotic biocatalysts in industrial and chemical applications. Consequently, there is tremendous interest in methodology that can use the power of genetics to develop strains with improved performance. For example, Metarhizium anisopliae is a broad host range entomopathogenic fungus currently under intensive investigation as a biologically based alternative to chemical pesticides. However, it use is limited by the relatively low tolerance of this species to abiotic stresses such as heat, with most strains displaying little to no growth between 35–37°C. In this study, we used a newly developed automated continuous culture method called the Evolugator™, which takes advantage of a natural selection-adaptation strategy, to select for thermotolerant variants of M. anisopliae strain 2575 displaying robust growth at 37°C. 相似文献14.
Sascha Al Dahouk Holger C Scholz Herbert Tomaso Peter Bahn Cornelia Göllner Wolfram Karges Bernd Appel Andreas Hensel Heinrich Neubauer Karsten Nöckler 《BMC microbiology》2010,10(1):269
Background
A commercial biotyping system (Taxa Profile™, Merlin Diagnostika) testing the metabolization of various substrates by bacteria was used to determine if a set of phenotypic features will allow the identification of members of the genus Brucella and their differentiation into species and biovars. 相似文献15.
Andreas M Boehm Stephanie Pütz Daniela Altenhöfer Albert Sickmann Michael Falk 《BMC bioinformatics》2007,8(1):214
Background
Mass spectrometry based quantification of peptides can be performed using the iTRAQ™ reagent in conjunction with mass spectrometry. This technology yields information about the relative abundance of single peptides. A method for the calculation of reliable quantification information is required in order to obtain biologically relevant data at the protein expression level. 相似文献16.
Mark WEJ Fiers Gijs A Kleter Herman Nijland Ad ACM Peijnenburg Jan Peter Nap Roeland CHJ van Ham 《BMC bioinformatics》2004,5(1):133
Background
Novel proteins entering the food chain, for example by genetic modification of plants, have to be tested for allergenicity. Allermatch™ is a webtool for the efficient and standardized prediction of potential allergenicity of proteins and peptides according to the current recommendations of the FAO/WHO Expert Consultation, as outlined in the Codex alimentarius. 相似文献17.
Danielle M Webster Chin Fen Teo Yuhua Sun Dorota Wloga Steven Gay Kimberly D Klonowski Lance Wells Scott T Dougan 《BMC developmental biology》2009,9(1):28-24
Background
The post-translational addition of the monosaccharide O-linked β-N-acetylglucosamine (O-GlcNAc) regulates the activity of a wide variety of nuclear and cytoplasmic proteins. The enzymes O-GlcNAc Transferase (Ogt) and O-GlcNAcase (Oga) catalyze, respectively, the attachment and removal of O-GlcNAc to target proteins. In adult mice, Ogt and Oga attenuate the response to insulin by modifying several components of the signal transduction pathway. Complete loss of ogt function, however, is lethal to mouse embryonic stem cells, suggesting that the enzyme has additional, unstudied roles in development. We have utilized zebrafish as a model to determine role of O-GlcNAc modifications in development. Zebrafish has two ogt genes, encoding six different enzymatic isoforms that are expressed maternally and zygotically. 相似文献18.
Background
Fungal biofilms are more resistant to anti-fungal drugs than organisms in planktonic form. Traditionally, susceptibility of biofilms to anti-fungal agents has been measured using the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxyanilide (XTT) assay, which measures the ability of metabolically active cells to convert tetrazolium dyes into colored formazan derivatives. However, this assay has limitations when applied to high C. albicans cell densities because substrate concentration and solubility are limiting factors in the reaction. Because mature biofilms are composed of high cell density populations we sought to develop a quantitative real-time RT-PCR assay (qRT-PCR) that could accurately assess mature biofilm changes in response to a wide variety of anti-fungal agents, including host immune cells. 相似文献19.
20.
J Theodore Phillips Edward Fox William Grainger Dianne Tuccillo Shifang Liu Aaron Deykin 《BMC neurology》2011,11(1):126