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1.
Oyster (Crassostrea plicatula) is widely distributed in coastal areas of China. We developed and evaluated simple sequence repeat (SSR) markers from expressed
sequence tags (ESTs) of Crassostrea gigas and to amplify EST-SSR in C. plicatula. Characteristics of eight EST-SSR loci were investigated using 37 wild-type C. plicatula individuals. The number of alleles per locus ranged from four to six. The observed heterozygosity (H
O) ranged from 0.1892 to 0.7027 (0.3919 on average) and the expected heterozygosity (H
E) ranged from 0.6068 to 0.7656 (0.7039 on average) (P < 0.05). The average observed heterozygosity was much lower than the average expected heterozygosity. All loci except C15
departed from Hardy-Weinberg equilibrium (P < 0.05) significantly. Contribution of the eight EST-SSR primers presented here will provide necessary and powerful molecular
tools for management and conservation studies on the species of oysters in the future. 相似文献
2.
Shui Ming Zhang Chang Jie Xu Zhong Shan Gao Kun Song Chen Guo Yun Wang 《Conservation Genetics》2009,10(5):1605-1607
Chinese bayberry (Myrica rubra Sieb. & Zucc.) is one of the most important fruit tree crops in southern China. This paper reports the development of microsatellite
primers for Chinese bayberry. By screening the expressed sequence tag database and other sources, 11 polymorphic loci were
generated and primers were designed. Polymorphism of these 11 loci was assessed in 32 cultivars. All the 11 loci are polymorphic
and the number of alleles (A) ranged from 3 to 12, observed heterozygosity (H
O) and expected heterozygosity (H
E) ranged from 0.1250 to 0.9667 and 0.2359 to 0.8790, respectively, polymorphism information content ranged from 0.2285 to
0.8516. These microsatellite loci should be useful in the studies of genetic diversity of Myrica rubra and will provide useful implications for resource conservation. 相似文献
3.
Oyster (Crassostrea gigas) is widely distributed in coastal areas of world. We developed and evaluated simple sequence repeat (SSR) markers from expressed
sequence tags (ESTs) of C. gigas and to amplify EST-SSR in C. gigas. Characteristics of 11 EST-SSR loci were investigated using 45 C. gigas individuals. The number of alleles per locus ranged from two to thirteen. The observed heterozygosity (H
o) ranged from 0.0889 to 0.7333 and the expected heterozygosity (H
e) ranged from 0.0859 to 0.8981. Because of their high level of polymorphism, our 11 single-locus EST-SSR markers will be valuable
tools for research on mating system, population genetics and systemic evolution of oyster in the future. 相似文献
4.
MA. CARMEN A. ABLAN 《Molecular ecology resources》2006,6(1):170-172
Ten polymorphic microsatellites were isolated from the red‐bellied yellow tail fusilier Caesio cuning, a reef‐associated fish which occurs widely in the Indo‐Pacific region. The species is exploited by both small‐scale and commercial fisheries. Fifty individuals from six populations were genotyped using primers that reliably amplified 10 polymorphic loci. The number of alleles per locus ranged from three to 11. Observed heterozygosity (HO) and expected heterozygosity (HE) ranged between 0.400 to 0.880 and 0.337 to 0.843, respectively. These microsatellite loci may be used to study population structure, genetic diversity and connectivity of C. cuning in the range of its distribution. 相似文献
5.
S. Suresh J. -W. Chung G. -T. Cho J. -S. Sung J. -H. Park J. -G. Gwag 《Plant biosystems》2013,147(4):635-644
We assessed the molecular genetic diversity and population structure of Amaranthus species accessions using 11 simple sequence repeat markers. A total of 122 alleles were detected, and the number of alleles per marker (NA) ranged from 6 to 21 with an average of 11.1 alleles. The frequency of major alleles per locus ranged from 0.148 to 0.695, with an average value of 0.496 per marker. The overall polymorphic information content values were 0.436–0.898, with an average value of 0.657. The observed heterozygosity (HO) and expected heterozygosity (HE) ranged from 0.056 to 0.876 and from 0.480 to 0.907, with average values of 0.287 and 0.698, respectively. The average HO (0.240) was lower than the HE and gene flow (Nm), and showed substantial genetic variability among all populations of amaranth accessions. The sample groupings did not strictly follow the geographic affiliations of the accessions. A similar pattern was obtained using model-based structure analysis without grouping by species type. Knowledge of the genetic diversity and population structure of amaranth can be used to select representative genotypes and manage Amaranthus germplasm breeding programs. 相似文献
6.
Jürgen Geist Oswald Rottmann Wolfgang Schrder Ralph Kühn 《Molecular ecology resources》2003,3(3):444-446
Freshwater pearl mussels (Margaritifera margaritifera L.) are among the most critically endangered freshwater invertebrates. We describe the isolation and characterization of the first microsatellite markers for this species, which were obtained by screening 4900 recombinant clones from two genomic libraries. Thirteen loci revealed polymorphisms as demonstrated on 42 tested individuals from four river drainages. Allelic richness ranged from two to 12 alleles and averaged 6.8 alleles per locus with heterozygosity levels varying from 0 to 0.850 for observed heterozygosity (HO) and from 0.174 to 0.850 for expected heterozygosity (HE). Deficiency of heterozygous genotypes was observed in eight of 13 loci. 相似文献
7.
The present study reports isolation and characterization of 11 polymorphic microsatellite markers for Nycticorax nycticorax. A total of 82 alleles were detected with an average of 7.5 alleles per locus. The observed heterozygosity (H
O) ranged from 0.25 to 1.00, and the expected heterozygosity (H
E) ranged from 0.51 to 0.88. Analyses revealed no evidence for Linkage disequilibrium between any two loci, and only one locus
was significantly deviated from HWE with the estimation of exact P values by the Markov chain method (P < 0.001). The 11 loci were successfully amplified in 11 other Ardeidae species. These results demonstrate these markers can
be used for the study of intra- and inter-specific genetic diversity. 相似文献
8.
Eleven polymorphic dinucleotide (GA and CA) microsatellites were isolated and characterized in the mosquito Anopheles sinensis; this species is distributed over the East Asia region and is a primary vector of malaria, particularly in Korea. The number of alleles per locus ranged from 4 to 13. The observed heterozygosity (HO) and expected heterozygosity (HE) varied from 0.30 to 0.89 and from 0.59 to 0.90, respectively. These microsatellites could be useful in studying the evolution of the widely distributed A. sinensis in diverse environments. 相似文献
9.
We have isolated and characterized twelve novel polymorphic microsatellite loci from Scapharca subcrenata to analyse the population structure. The number of observed alleles per locus ranged from 3 to 17. Observed heterozygosity
(H
O) ranged from 0.321 to 0.929. Cross-species amplification was tested successfully in three other bivalve species. These microsatellite
markers will be useful for genetic diversity studies of S. subcrenata and other Lamellibranchia species. 相似文献
10.
Genetic diversity of common carp from two largest Chinese lakes and the Yangtze River revealed by microsatellite markers 总被引:9,自引:1,他引:8
Six polymorphic microsatellites (eight loci) were used to study the genetic diversity and population structure of common carp
from Dongting Lake (DTC), Poyang Lake (PYC), and the Yangtze River (YZC) in China. The gene diversity was high among populations
with values close to 1. The number of alleles per locus ranged from 2 to 11, and the average number of alleles among 3 populations
ranged from 6.5 to 7.9. The mean observed (H
O) and expected (H
E) heterozygosity ranged from 0.4888 to 0.5162 and from 0.7679 to 0.7708, respectively. Significant deviations from Hardy–Weinberg
Equilibrium expectation were found at majority of the loci and in all three populations in which heterozygote deficits were
apparent. The analysis of molecular variance (AMOVA) indicated that the percent of variance among populations and within populations
were 3.03 and 96.97, respectively. The Fst values between populations indicated that there were significant genetic differentiations for the common carp populations
from the Yangtze River and two largest Chinese freshwater lakes. The factors that may result in genetic divergence and significant
reduction of the observed heterozygosity were discussed. 相似文献
11.
Characterization of new microsatellite loci in Pieris amamioshimensis (Ericaceae), a species nearly extinct in the wild
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A set of 11 polymorphic microsatellite markers has been developed and characterized for the critically endangered species Pieris amamioshimensis. Fifty‐nine individuals of an ex‐situ population were used to identify these markers. The total number of alleles for each locus ranged from 3 to 9, with an average of 5.4. The expected heterozygosities (HS) and observed heterozygosities (HO) ranged from 0.47 to 0.77 and 0.22 to 0.88, respectively. In total, four loci exhibited significant deviations from Hardy–Weinberg equilibrium: two loci showed significant heterozygosity excess and the other two loci showed significant heterozygosity deficit. The polymorphism information content (0.43 ≤ PIC ≤ 0.73), the probability of exclusions (PE1 = 0.9565, PE2 = 0.9969 and PE3 = 0.9999) and probabilities for identity (PI = 3.78 × 10?9 and PI‐Sib = 2.35 × 10?4) suggest that these markers are useful for estimating not only genetic diversity but also parentage, for the ex‐situ conservation management of populations. 相似文献
12.
E.L.S. Matos E.J. Oliveira O.N. Jesus J.L.L. Dantas 《The Annals of applied biology》2013,163(2):298-310
We assessed the genetic diversity of 96 papaya accessions by molecular characterisation using microsatellite markers. Fifteen polymorphic primers were selected. Accessions, which were classified as Common, Formosa and Solo according to fruit types, were evaluated for allele frequency, heterozygosity, polymorphism information content (PIC), inbreeding coefficient (f) and the genetic diversity structure. Fifteen primers amplified 68 alleles with an average of 4.53 per locus. PIC values ranged from 0.19 to 0.69. The observed heterozygosity (HO) was low for all selected microsatellites. High f estimates (0.58) and excess of homozygotes indicated inbreeding, mainly caused by the tendency to select hermaphrodite plants for succeeding generations. Analysis of molecular variance showed that most of the variation (98%) resides within subpopulation. The genetic analysis based on Bayesian statistics proved to be sensitive enough to detect relationships among the papaya accessions, grouping them into six clusters, irrespective of their classification types. 相似文献
13.
Freshwater molluscs are globally critically threatened, yet little is known about their genetic diversity. We describe the
isolation and characterization of the first microsatellite markers for the European swan mussel, Anodonta cygnea. Nine loci were polymorphic in a panel of 110 individuals from seven German and Estonian populations. Number of alleles ranged
from 2 to 14 and heterozygosity levels ranged from 0.021 to 0.498 for H
o and from 0.069 to 0.634 for H
e. Deficiency of heterozygous genotypes was observed in six loci. The length of the microsatellite repeat motives was positively
correlated with the allele numbers in the respective loci. 相似文献
14.
L. REX MCALILEY RAYMOND E. WILLIS MICHAEL R. J. FORSTNER TRINA GUERRA LLEWELLYN D. DENSMORE 《Molecular ecology resources》2006,6(3):759-761
We characterize eight polymorphic microsatellite loci for the San Esteban chuckwalla, Sauromalus varius, which are being used as markers in a study of the genetic results from a decade of captive breeding in this endangered species. Observed heterozygosity estimates for S. varius ranged from 0.00 to 0.30, whereas expected heterozygosity estimates ranged from 0.06 to 0.58. 相似文献
15.
The aim of this study was to determine microsatellite polymorphism in ostriches and using it in creation the genetic map of
the ostrich. The polymorphism analysis covered 30 microsatellite markers characteristic of ostrich, for the CAU (China Agricultural
University) group. The material consisted of 150 ostriches (Struthio camelus). The 30 microsatellite loci was examined and a total of 343 alleles was identified. The number of alleles at a single locus ranged from 5 at locus CAU78 to 34 at locus CAU85. The values for the observed heterozygosity Ho ranged from 0.467 (locus CAU78) to 0.993 (locus CAU16), whereas for the expected heterozygosity He - from 0.510 (locus CAU78) to 0.953 (locus CAU85). Analyzing the individual loci, the highest PIC value, more than 0.7 was observed for: loci CAU85 (0.932), CAU64 (0.861) and CAU32, 75 (0.852), respectively. It should be noted, that the microsatellite markers used
in our study were very polymorphic as evidenced by the large number of detected alleles and high rates of heterozygosity,
PIC and PE as well. The analysed microsatellite markers may be used in genetic linkage mapping of ostrich, the construction
of a comparative genetic map with other ratites, such as emu and rhea, and population genetics studies or phylogenetic studies
of these birds. 相似文献
16.
Characterization of microsatellite markers in the mosquito Ochlerotatus caspius (Diptera: Culicidae)
Seven polymorphic microsatellite loci were developed for the mosquito species Ochlerotatus caspius, using an enriched genomic library. The number of alleles per locus varied between two and 11; the expected heterozygosity (HE) ranged from 0.18 to 0.77. These microsatellite primers should prove useful for population genetic studies of this mosquito species. 相似文献
17.
Matthew E. Horning Scott C. Maloney Michael S. Webster 《Molecular ecology resources》2003,3(3):412-413
Here we describe the characterization of six polymorphic microsatellite loci for Lilium philadelphicum (Liliaceae). Polymorphism levels ranged from 7 to 30 alleles per locus with a mean number of 15 alleles per locus. Observed heterozygosity (HO) ranged from 0.17 to 0.82 and did not deviate from Hardy–Weinberg expectations in the three western Montana populations included in the analysis. These loci are proving useful in studying gene flow between populations of this species distributed across its range in North America. 相似文献
18.
LI JIN HELEN JANE TEMPLE JOSEPH IVAN HOFFMAN SHENGJIANG TAN WILLIAM AMOS 《Molecular ecology resources》2006,6(3):862-864
We isolated and characterized six polymorphic microsatellite markers for the white‐breasted thrasher from genomic libraries enriched for (AC)n, (GT)n, (CAAA)n, (TTTC)n, (GAC)n, (CT)n and (TTTG)n microsatellites. The number of alleles per locus ranged from two to seven. Observed heterozygosity (HO) ranged from 0.30 to 0.85. 相似文献
19.
Four new microsatellite primer pairs were developed in tree peony (Paeonia suffruticosa) based on the database mining and other twenty-six primer pairs by fast isolation by AFLP of sequences containing repeats
(FIASCO) method. The polymorphism of each locus was further evaluated in 40 individuals of one population plus 5 tree peony
related species. The number of alleles per locus ranged from 3 to 7 and the expected (He) and observed (Ho) heterozygosity at each locus ranged from 0.42 to 0.78 and 0.28 to 0.59, respectively. These microsatellite markers will
be useful for investigating genetic diversity and studies of population genetic structure of tree peony. 相似文献
20.
Noble crayfish (Astacus astacus) is listed as vulnerable by the IUCN Red List of Threatened Species in Europe. However, very little is known about genetic
diversity and structuring of noble crayfish populations, mainly because of the lack of informative genetic markers. We describe
the isolation and characterization of the first microsatellite markers for this species, which were obtained by screening
4,000 recombinant clones. Eight loci revealed polymorphisms in a panel of 172 individuals from seven populations in Northern
Europe. Number of alleles per locus ranged from two to 10 (average 4.4) and heterozygosity levels among populations varied
from 0 to 0.80 for H
o and from 0 to 0.72 for H
e. 相似文献