野外捕捉,翻过去的一页

去非洲之前.以为捕捉动物就是用麻醉枪.其实当地的捕猎队是拿粉套杆乘粉汽车追.追上之后把绳套甩出去套住动物。斑马被套住后.当地的捕猎队马上跳下车.几个人一起把它拥住。从发现动物到追上.到恋上、装笼.整个过程也就是二三十分钟.可谓速战速决。野外捕捉,翻过去的一页~~     

化石初探——记古生态学野外实习

对第一次出野外采集化石的我而言,2010年10月24日的军庄镇之行无疑成了记忆中难以忘怀的经历。在这次野外之行的几个星期以前,我们就得知“古生态学课”将有外出实习的机会,所以这一天早就成了我和同学们心中期盼的日子。当它真正到来的时候,每个人在心理上都做好了准备,即便遇上了阴雨天气,大家还是兴致勃勃地准时聚集到了约定地点。潘云唐老师分派好地质锤“责任人’之后,我们便搭上了前往北京郊外的大巴。     

记杨老的最后一次野外考察

1975年的春天,北京的气候温暖清新。应重庆市文化局和重庆博物馆的邀请,古脊椎所派出以杨老为团长、张力彬为副团长,业务人员有赵喜进和我的四人代表团参加重庆博物馆第一架恐龙开幕展。同时。杨老要到万县卡氏兽产地和自贡恐龙产地考察,所领导让我具体负责照顾杨老的生活等事情．所以在重庆地区的近两个星期时间里,我与杨老形影不离。     

西夏行--记我的第一次野外考察

八月十五号贾哥、卢姐、师妹卢静和我一行四人踏上了由北京开往宁夏方向的K43次列车,开始了我研究生生涯的第一次野外考察之旅。由于是第一次出野外,所以我和师妹都比较兴奋,一路上不停的向贾哥和卢姐问这问那。我和师妹都师从于我国著名的古鱼类学家朱敏研究员,主要从事古生代     

我要去那遥远的非洲——记伦敦动物园

初中学英语时，非常喜欢zoo这个词，因为简单，而且含义有趣。迪斯尼动画片《Lady and Tramp》里，女主角Lady（一只小狗）被误解她的主要戴上嘴套，男主角Tramp（当然也是一只小狗）英雄救美，带她到动物园去寻求帮助，最后请河狸把嘴套的皮带咬断，皆大欢开赴。     

提倡一种生物学野外实习的新方式

生物学专业野外实习中对动植物标本的过度采集已经对野生生物物种的多样性产生了一定程度的破坏,为此笔者提出一种非采集的野外实习新方式和相应的实习方法与措施。     

野外生存——人类曾经的必修课

<正>野外生存是指人类在非生活环境下,最大限度地维持生命力的行为。关于野外生存起源的问题,许多学者都有不同的见解。目前,在国内外文献记载和网络流行说法中,比较普遍的意见是缘于第二次     

cDNA捕捉法——从大的基因组区域直接分离编码序列

cDNA捕捉法或cDNA直选法是一种以表达为基础的基因分离技术,直接利用目的区域的基因组DNA捕捉cDNA,快速从大的基因组区域分离表达序列。该法已成功地应用于定位克隆和详尽的转录图谱的构建。     

一种提取动物基因组总DNA的野外样品保存方法

为了确定一种方便的野外动物样品保存方法,以新鲜材料作对照,从-20℃冰箱、70%乙醇、含50mmol/L EDTA的70%乙醇、95%乙醇、液氮处理的高原鼠肌肉和肝脏组织中提取基因组总DNA。通过琼脂糖凝胶电泳和紫外分光光度计对提取的基因组总DNA质量进行检测。结果显示:相同处理的肝脏DNA产量大,肌肉组织提取的DNA质量好;各种保存方法提取的DNA降解程度依次为,-20℃冰箱、70%乙醇>含50mmol/L EDTA的70%乙醇、95%乙醇>液氮>新鲜。选择新鲜肌肉和95%酒精处理的肌肉样品提取的总DNA作模板,进行微卫星PCR扩增,均可获得清晰的电泳带。将该方法用于高原鼢鼠,进行线粒体12S rRNA、Cytb和D-loop区测序,结果显示该方法保存的样品与新鲜样品没有差别。因此,在野外用95%乙醇固定肌肉样品是一种可行的样品保存方法。     

野外生物是我的朋友——青少年时期的蕾切尔&#183;卡逊

使她与众不同的，是她自己的决定。在孩提时代，蕾切尔&#183;卡逊就决心要做一名作家。文学的天赋，也许是天才，以及充满进取心的头脑使她产生了这种意愿。但这一切的基础，是极度热切的渴望。     

Unknown and Already Known Thyroid Abnormalities in Primary Hyperparathyroidism

Objective: Primary hyperparathyroidism (PHPT) and thyroid diseases are highly prevalent in the general population, but the putative link between the 2 conditions remains unclear.Methods: A monocentric consecutive series of 434 patients with PHPT was retrospectively evaluated by lab and ultrasonography to look for thyroid abnormalities. Patients were classified in 3 groups: without thyroid abnormalities (group 1, n = 171), with thyroid diseases not previously known (group 2a, n = 69), and thyroid diseases previously known (group 2b, n = 194).Results: In terms of thyroid disease, no significant difference was found between groups 2a and 2b, except for the significantly larger number of patients with toxic nodular goiter in group 2b. PHPT was more frequently symptomatic in group 2a than in group 2b, despite no differences in serum calcium, creatinine, parathyroid hormone (PTH), or 25-hydroxyvitamin D (25OHD) levels.Conclusion: A total of 60% of PHPT patients had a thyroid disease that was unknown prior to PHPT diagnosis in almost one-third of cases. The newly diagnosed and previously known thyroid diseases were similar, both mostly affecting postmenopausal females.Abbreviations: Ab = antibody; aPHPT = asymptomatic PHPT; 25OHD = 25-hydroxyvitamin D; PHPT = primary hyperparathyroidism; PTH = parathyroid hormone; Tg = thyroglobulin; TPO = thyroperoxidase; TSH = thyroid-stimulating hormone; US = ultrasound     

Capture of a dimeric intermediate during transthyretin amyloid formation

Point mutations in the human plasma protein transthyretin are associated with the neurological disorder familial amyloidosis with polyneuropathy type 1. The disease is characterized by amyloid fibril deposits causing damage at the site of deposition. Substitution of two amino acids in the hydrophobic core of transthyretin lead to a mutant that was very prone to form amyloid. In addition, this mutant has also been shown to induce a toxic response on a neuroblastoma cell line. Renaturation of the transthyretin mutant at low temperature facilitated the isolation of an amyloid-forming intermediate state having the apparent size of a dimer. Increasing the temperature effectively enhanced the rate of interconversion from a partly denatured protein to mature amyloid. Using circular dichroism the beta-sheet content of the formed mature fibrils was significantly lower than that of the native fold of transthyretin. Morphology studies using electron microscopy also indicated a temperature-dependent transformation from amorphous aggregates toward mature amyloid fibrils. In addition, 1-anilino-8-naphtalenesulfonate fluorescence studies suggested the loss of the thyroxin-binding channel within both the isolated intermediate and the mature fibrils.     

Collaborative Moments. Expanding the Anthropological Field through Cross-Disciplinary Practice

ABSTRACT

This article explores some implications of cross-disciplinarity, as experienced in practice. Anthropologists are used to fuse different styles of reasoning by integrating the points of view and unspoken certainties of their partners in the field into their analysis. Fieldwork can be seen as an experiment in real time, where insights gained intersubjectively gradually shape up as knowledge through analysis. This line of thought is brought to bear on a discussion of collaboration between anthropologists, archaeologists, and biologists in North West Greenland. Through actual experiences from the field, this article shows how knowledge generated on the edge of one’s familiar disciplinary territory may both expand and intensify the anthropological field. Collaborative moments are seen to make new anthropological insights emerge through the co-presence of several analytical perspectives in the field.