共查询到20条相似文献,搜索用时 31 毫秒
1.
In primary cultures of adult rat hepatocytes, dexamethasone (10?5M) induced tyrosine aminotransferase (TAT) 24 h after its addition. Glucagon (10?7M) alone had no effect, but strongly enhanced the induction by dexamethasone. Glucagon could be replaced by butyryl cyclic-AMP (10?4M), which caused about 20-fold increase in activity. In contrast to many previous reports that insulin induced TAT activity and , it inhibited the inductions of TAT by dexamethasone and dexamethasone plus glucagon 24 h after its addition. However, insulin significantly induced TAT activity in the early pahse, 4 h after its addition. Dose-response curves of the effect of insulin on TAT activity showed reverse relations to activity in early and late phase. These results show that TAT activity is regulated by insulin in a two phase fashion. 相似文献
2.
Brief treatment of hepatoma cells in monolayer culture with concanavalin A causes a decrease in tyrosine aminotransferase specific activity that is thought to be a rapid, reversible inactivation of the enzyme (T.V. Gopalakrishnam and E.B. Thompson 1977 J. Biol. Chem. , 2717–2725). We confirm this decrease, but attribute it to an increased leakage of cellular protein from concanavalin A-treated monolayer cultures during the harvesting procedure. If the cells are washed free of medium and lysed , by freezing and thawing them, or by treating them with buffer containing a nonionic detergent, equal amounts of tyrosine aminotransferase are found in concanavalin A-treated and untreated cells. If cells are harvested by scraping them from the substrate, some tyrosine aminotransferase is lost into the buffer used to collect the cells. Treatment of cells with concanavalin A markedly increases the amount of enzyme lost during this procedure, and results in a low enzyme content in the washed cells. No inactivation occurs, however, because the total amount of tyrosine aminotransferase present in the cell pellet and the wash buffer is equal for treated and untreated cells. 相似文献
3.
4.
A particulate factor of rat liver is described which interconverts three forms of rat liver cytosolic tyrosine aminotransferase with no alteration of enzyme activity. The factor appears to be a heat- and pH-sensitive lysosomal protein. The interconversion process is stimulated by 2.5 mM MgCl2 and 2.5 mM ATP. Asparate aminotransferase multiple forms are also susceptible to interconversion by the lysosomal factor. The properties of the factor explain several anomalous effects of manipulation on the tyrosine aminotransferase forms which have been reported in the literature and implicate the form interconversion in the degradation of tyrosine aminotransferase. 相似文献
5.
6.
7.
Jørgen Vinten 《生物化学与生物物理学报:生物膜》1978,511(2):259-273
The transport of in white fat cells was measured under equilibrium exchange conditions at concentrations up to 50 mM with a previously described method (Vinten, J., Gliemann, J. and Østerlind, K. (1976) J. Biol. Chem. 251, 794–800). Under these conditions the main part of the transport was inhibitable by cytochalasin B. The inhibition was found to be of competitive type with an inhibition constant of about 2.5 · 10?7 M, both in the absence and in the presence of insulin (1μM). The cytochalasin B-insensitive part of the permeability was about 2 · 10?9 cm · s?1, and was not affected by insulin. As calculated from the maximum transport capacity, the half saturation constant and the volume/ surface ratio, the maximum permeability of the fat cell membrane to at 37°C and in the presence of insulin was 4.3 · 10?6 cm · s?1. From the temperature dependence of the maximum transport capacity in the interval 18–37°C and in the presence of insulin, an Arrhenius activation energy of was found. The corresponding value was in the absence of insulin. The half saturating concentration of was about 6 mM in the temperature interval used, and it was not affected by insulin, although this hormone increased the maximum transport capacity about ten-fold to 1.7 mmol · s?1 per 1 intracellular water at 37°C. 相似文献
8.
K Nagaiah P MacDonnell G Guroff 《Biochemical and biophysical research communications》1977,75(4):832-837
The addition of dexamethasone and nerve growth factor to organ cultures of superior cervical ganglia from young rats induces the synthesis of tyrosine hydroxylase. The combination of nerve growth factor and dexamethasone produces a differential rate of tyrosine hydroxylase synthesis which approaches that obtained by the administration of nerve growth factor. 相似文献
9.
10.
11.
M Kuwano H Taniguchi M Ono H Endo Y Ohnish 《Biochemical and biophysical research communications》1977,75(1):156-162
An mutant (JE14373) carrying decreased stability of stable RNA species was found to have altered electrophoretic mobility of a 30S ribosomal protein (S10). Recombinants covering gene (76 min on linkage map by Bachmann, Low and Taylor, 1976 (ref. 1)) obtained from a cross of CSH64 × JE14373, restored normal S10 protein. The size analysis of RNAs labeled for 15 min with [3H]uridine showed 50 to 60 % decrease of 16S RNA in this mutant strain, but almost no decrease of 23S RNA at 10 or 40 min after addition of rifampicin. On the other hand, no change was observed in the stability of both rRNA pieces in its parental PA3092 strain even at 40 min after addition of rifampicin. 相似文献
12.
13.
14.
15.
16.
17.
《Phytochemistry》1986,25(5):1021-1030
18.
19.