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A mouse renin-1 gene promoter fragment, normally inactive in B-cells, becomes a potent promoter in these cells after insertion of the highly conserved decanucleotide (dc/cd sequence) of immunoglobulin heavy and light chain promoters [(1987) EMBO J. 6, 1685-1690]. We observe retarded complexes of the same electrophoretic mobility when the cd-containing renin promoter fragment or an authentic immunoglobulin heavy chain promoter fragment is incubated with a nuclear extract from myeloma cells, suggesting that the renin promoter is activated due to its acquired ability to bind a B-cell-specific positive factor. No retarded complexes are observed with the original renin promoter fragment thus questioning the presence of a repressor as an explanation for its lack of activity in B-cells. 相似文献
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Regulation of the immunoglobulin gene transcription 总被引:2,自引:0,他引:2
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Alison M Clargo Ashley R Hudson Welcome Ndlovu Rebecca J Wootton Louise A Cremin Victoria L O'Dowd Carla R Nowosad Dale O Starkie Sophie P Shaw Joanne E Compson Dominic P White Brendon MacKenzie James R Snowden Laura E Newnham Michael Wright Paul E Stephens Meryn R Griffiths Alastair DG Lawson Daniel J Lightwood 《MABS-AUSTIN》2014,6(1):143-159
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T. Venkat Gopal Tom Polte Patrick Arthur Michael Seidman 《In vitro cellular & developmental biology. Plant》1989,25(12):1147-1154
Summary A hybrid cell line was constructed by fusion of mouse L-cells with an NIH3T3 cell line derivative containing a hybrid gene
consisting of the mouse immunoglobulin kappa (IgK) variable gene promoter linked to theEscherichia coli gpt gene. Such hybrids grew to a much higher density compared to either of the parental cell lines. The utility of this cell
line as a host to express foreign genes was tested by the expression of TGF-β cDNA using the cytomegalovirus promoter. The
vector also contained the human dihydrofolate reductase (DHFR) gene driven by SV40 early promoter, to allow for the amplification
of the transfected gene. Initial transformants, selected at 100 nM methotrexate (MTX), were subsequently selected for resistance to a higher concentration of MTX (2 μM). Such clones expressed an increased level of TGF-β when compared to the initial transformants. Both the initial transformants
and the clones with the amplified DHFR gene produced TGF-β in an acid-activatable precursor form. This mouse hybrid host cell
line also allowed the expression of foreign genes cloned in an eukaryotic expression vector with the mouse IgK variable region
promoter and human growth hormone as the reporter gene, whereas such vectors did not function in CHO cells. The mouse hybrid
cell line was also found to be capable of being used with a broad range of promoters. 相似文献
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