共查询到20条相似文献,搜索用时 15 毫秒
1.
Desjardins CA Champion MD Holder JW Muszewska A Goldberg J Bailão AM Brigido MM Ferreira ME Garcia AM Grynberg M Gujja S Heiman DI Henn MR Kodira CD León-Narváez H Longo LV Ma LJ Malavazi I Matsuo AL Morais FV Pereira M Rodríguez-Brito S Sakthikumar S Salem-Izacc SM Sykes SM Teixeira MM Vallejo MC Walter ME Yandava C Young S Zeng Q Zucker J Felipe MS Goldman GH Haas BJ McEwen JG Nino-Vega G Puccia R San-Blas G Soares CM Birren BW Cuomo CA 《PLoS genetics》2011,7(10):e1002345
Paracoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, a health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, a dimorphic fungus in the order Onygenales, is coupled with a thermally regulated transition from a soil-dwelling filamentous form to a yeast-like pathogenic form. To better understand the genetic basis of growth and pathogenicity in Paracoccidioides, we sequenced the genomes of two strains of Paracoccidioides brasiliensis (Pb03 and Pb18) and one strain of Paracoccidioides lutzii (Pb01). These genomes range in size from 29.1 Mb to 32.9 Mb and encode 7,610 to 8,130 genes. To enable genetic studies, we mapped 94% of the P. brasiliensis Pb18 assembly onto five chromosomes. We characterized gene family content across Onygenales and related fungi, and within Paracoccidioides we found expansions of the fungal-specific kinase family FunK1. Additionally, the Onygenales have lost many genes involved in carbohydrate metabolism and fewer genes involved in protein metabolism, resulting in a higher ratio of proteases to carbohydrate active enzymes in the Onygenales than their relatives. To determine if gene content correlated with growth on different substrates, we screened the non-pathogenic onygenale Uncinocarpus reesii, which has orthologs for 91% of Paracoccidioides metabolic genes, for growth on 190 carbon sources. U. reesii showed growth on a limited range of carbohydrates, primarily basic plant sugars and cell wall components; this suggests that Onygenales, including dimorphic fungi, can degrade cellulosic plant material in the soil. In addition, U. reesii grew on gelatin and a wide range of dipeptides and amino acids, indicating a preference for proteinaceous growth substrates over carbohydrates, which may enable these fungi to also degrade animal biomass. These capabilities for degrading plant and animal substrates suggest a duality in lifestyle that could enable pathogenic species of Onygenales to transfer from soil to animal hosts. 相似文献
2.
Minakawa Tomoko Shumoto Godai Kezuka Chiho Izawa Takeshi Sasaki Kyoko Yamaguchi Sayaka Kamezaki Naoki Yamate Jyoji Konno Toshihiro Sano Ayako Itano Eiko Nakagawa Wada Shinpei Willson Chris Ueda Keiichi 《Mycopathologia》2020,185(6):1013-1020
Mycopathologia - The skin disease paracoccidioidomycosis ceti occurs in several dolphin species globally. Infection by the unculturable fungi Paracoccidioides brasilensis or other Paracoccidioides... 相似文献
3.
Ayaz Ghani Molly Weinberg Nusrat Pathan Ramapriya Vidhun Steven Sieber 《Mycopathologia》2018,183(6):973-977
Paracoccidioides infection is a rare entity in the USA. This dimorphic fungus is found in Central and South America and is thought to be acquired by inhalation through the soil. We report a case of Paracoccidioides brasiliensis infection presenting as a clavicular bone lesion, peripancreatic mass, and various skin lesions. A 35-year-old man with a history significant for Hodgkin lymphoma presented with a left clavicular mass that was suspected clinically and radiologically as recurrent Hodgkin lymphoma. He was not experiencing any associated symptoms and was undergoing chemotherapy treatment for his known Hodgkin disease. On CT imaging, the mass was seen as a lytic bone lesion with an overlying soft tissue mass. This was biopsied and histologically diagnosed as a Paracoccidioides brasiliensis infection with associated necrotizing granulomatous inflammation. Also found on the CT scan was an enlarging peripancreatic mass which on endoscopic biopsy had similar histologic findings. In conclusion, this report presents a rare case of Paracoccidioides brasiliensis infection mimicking recurrent Hodgkin lymphoma. 相似文献
4.
Resumen Cuarenta y cinco muestras de tierra, preferentemente de cafetales a más de 500 m de altitud sobre el nivel del mar, de varias regiones de Venezuela fueron inoculadas por vía endovenosa en ratones.No pudo aislarse elParacoccidioides brasiliensis.Se discuten brevemente las posibles causas de los resultados negativos y se proponen otras téchnicas para seguir intentando la determinación del habitat deParacoccidioides brasiliensis.
Summary Forty five soil samples, most of them from coffee plantations at more than 500 m altitude, from several parts from Venezuela were inoculated intravenously into mice. Paracoccidioides brasilicnsis was not isolated.The authors discuss the possible reasons of the negative results and propose other techniques to apply in the future in order to isolateParacoccidioides brasilicnsis from nature.
Résumé Quarante cinq échantillons de terre furent inoculés par voie intravéneuse — des souris. Les échantillons provenaient principalement de plantations de café — plus de 500 m d'altitude de plusieurs régions du Venezuela.LeParacoccidioides brasiliensis n'a pu etre isolé.Les auteurs discutent les causes éventuelles de cet échec et proposent des méthodes différentes pour reprendre la recherche duParacoccidioides brasiliensis dans la nature.相似文献
5.
Graziella Hanna Pereira Aline Queiroz Santos Miriam Park Patricia Rady Muller Soraia Padua Raquel Ferrari Marchesi Vera Lucia Aldred 《Mycopathologia》2010,170(4):259-261
Paracoccidioides brasiliensis rarely shows bone marrow involvement and its response to treatment with itraconazole in children needs further assessment. We describe here a child with a juvenile disseminated form of paracoccidioidomycosis, which showed reticuloendothelial system involvement and the presence of Paracoccidioides brasiliensis in the bone marrow. The patient showed an effective and rapid response to itraconazole therapy. 相似文献
6.
I Campo-Aasen 《Sabouraudia》1985,23(2):101-105
The succinate dehydrogenase activity of Paracoccidioides brasiliensis was investigated histochemically by electron microscopy. The reaction product of this enzyme was demonstrated in some membranous structures of organelles interpreted as mitochondria. This enzyme shows very active oxido-reduction in Paracoccidioides brasiliensis mitochondria during, 3, 6, and and 9 days of culture. 相似文献
7.
Carrero LL Niño-Vega G Teixeira MM Carvalho MJ Soares CM Pereira M Jesuino RS McEwen JG Mendoza L Taylor JW Felipe MS San-Blas G 《Fungal genetics and biology : FG & B》2008,45(5):605-612
By means of genealogical concordance phylogenetic species recognition (GCPSR), we have investigated coding and non-coding regions from various genes and the ITS sequences of 7 new and 14 known isolates of Paracoccidioides brasiliensis. Such isolates grouped within the three phylogenetic groups recently reported in the genus Paracoccidioides, with one single exception, i.e., Pb01, a strain that has been the subject of intense molecular studies for many years. This isolate clearly separates from all other Paracoccidioides isolates in phylogenetic analyses and greatly increases the genomic variation known in this genus. 相似文献
8.
Ladislao Pollak 《Mycopathologia》1971,45(3-4):217-219
In soil extract agar and in Bennett medium abundant aleuriospores ofParacoccidioides brasiliensis have been observed. The possibility that these spores could be the infecting elements for man is discussed.
Resumen Se han observado abundantes aleuriosporas deParacoccidioides brasiliensis en agar extracto de tierra y en medio de Bennett. Se discute la posibilidad de que éstas esporas puedan ser los elementos que infectan al hombre.相似文献
9.
dos Santos VM Xavier RM Cortes JA Osterne EM de Worisch Ferreira Lopes M 《Mycopathologia》2008,166(3):155-158
Neuroparacoccidioidomycosis is the central nervous system involvement by Paracoccidioides brasiliensis, a condition that may be underdiagnosed and has been scarcely reported. We describe a case of neuroparacoccidioidomycosis in a diabetic male with antecedent of heavy cigarette smoking and alcohol abuse. Thirty years before, he lived in the Amazon area and exerted rural activities in more recent years. The patient's main complaints were headache, visual deficit, hemiparesis, and weight loss. Imaging studies detected changes in the lungs and right adrenal gland, in addition to brain lesions. Paracoccidioides brasiliensis was found in tissue samples collected by the lung and brain biopsies. The patient is under ambulatory surveillance and in use of trimethoprim-sulfamethoxazole. 相似文献
10.
R. G. Baruzzi L. F. Marcopito N. S. Michalany J. Livianu N. R. S. Pinto 《Mycopathologia》1981,74(1):51-54
In 1931 the Brasilian doctor Jorge Lobo examined a patient from the Amazon Basin who had nodular confluent skin lesions over the lumbosacral region and encountered a new fungus pathogenic for man Paracoccidioides loboi. This classification was based on the morphological aspect of the parasite which was very similar to Paracoccidioides brasiliensis the causative agent of a systemic mycosis Paracoccidioidomycosis or South American Blastomycosis. Other authors use the name Loboa loboi. The final determination of the name must await cultivation of the fungus. 相似文献
11.
Ueda Keiichi Nakamura Ichiro Itano Eiko Nakagawa Takemura Kazunori Nakazato Yasutomo Sano Ayako 《Mycopathologia》2017,182(9-10):937-946
Mycopathologia - “Paracoccidioidomycosis ceti” is a rare zoonotic fungal infection affecting dolphins and is endemic worldwide. The causative agents are Paracoccidioides species;... 相似文献
12.
L M Carbonell 《Journal of bacteriology》1969,98(3):1395-1396
The occurrence of a live hypha inside a dead yeast during the transformation from yeast to mycelium of Paracoccidioides brasiliensis is described. 相似文献
13.
14.
A recent species status investigation of the pathogenic fungus Paracoccidioides brasiliensis suggested the existence of three cryptic species. In the present study, the sequences of the PRP8 intein from P. brasiliensis isolates belonging to the three described genetic groups and two unidentified isolates were determined and analyzed in order to check their functionality and usefulness for species identification. All the isolates presented a full-length intein, although the Endonuclease domain seems to be inactive due to substitutions in the second essential aspartic acid residue. Phylogenetic analysis by Maximum-Parsimony, Maximum Likelihood, and Bayesian analysis clearly separated the isolates from the three species and revealed a significant difference between the Pb01 isolate and the remaining ones. The Pb01 isolate does not belong to any of the groups previously described since it presented a high divergence level compared to the three different genetic groups, corroborating some previous studies that suggested this isolate represents a new species of Paracoccidioides. 相似文献
15.
Maluf ML Takahachi G Svidzinski TI Xander P Apitz-Castro R Bersani-Amado CA Cuman RK 《Revista iberoamericana de micología》2008,25(3):163-166
The natural compound ajoene (4,5,9- trithiadodeca-1,6,11-triene 9-oxide) is capable of controlling infection by Paracoccidioides brasiliensis in experimental models. Swiss mice were inoculated with 5.0 x 10e6 cells of the fungus Paracoccidioides brasiliensis Pb18 by intraperitoneal route and treated with ajoene. In weeks 2, 6, 10 and 13 of treatment, levels of anti-Pb antibodies were measured by the ELISA test and the animals were put down and their lungs, livers and spleens removed for histopathological analysis and determination of the number of viable fungus. The results show that experimental murine paracoccidioidomycosis was well established and that ajoene was capable of controlling the evolution of the disease, as it significantly reduced the levels of antibodies from the 10th week of treatment. 相似文献
16.
M?nica Sawan Mendon?a Terezinha S Pera?olli Mário León Silva-Vergara Sílvio C Ribeiro Rafael Faria Oliveira Rinaldo Poncio Mendes Virmondes Rodrigues Jr 《Memórias do Instituto Oswaldo Cruz》2015,110(6):781-785
Paracoccidioidomycosis (PCM) is caused by dimorphic fungi from
theParacoccidioides brasiliensis complex. Previous studies have
demonstrated that the severity of disease is associated with a T-helper 2 immune
response characterised by high interleukin (IL)-4 production. In the present study we
analysed two polymorphisms in the IL-4 gene (-590 C/T and intron-3
microsatellite) in 76 patients with PCM and 73 control subjects from an endemic area.
The production of IL-4 by peripheral blood mononuclear cells after antigen or
phytohaemagglutinin stimulation was determined by ELISA. A significant correlation
was observed between the RP2/RP2 intron-3 genotype and infection with
Paracoccidioides sp. (p = 0.011), whereas the RP1/RP1 genotype
was correlated with resistance. No significant correlation was observed for
the IL-4 promoter polymorphism. Furthermore, the low IL-4
expression observed in the control group compared with patients was associated with
the RP1/RP1 genotype. These results suggest that IL-4polymorphisms
might be associated with the ability of the host to control Paracoccidioides
sp. infection. The relevance of this polymorphism is supported by the
observation that patients with disease produce high levels of IL-4 following mitogen
or antigen stimulation. The IL-4 gene is located in the cytokine
cluster region of chromosome 5 where other polymorphisms have also been
described. 相似文献
17.
Enzymes in glycolysis and the citric acid cycle in the yeast and mycelial forms of Paracoccidioides brasiliensis 总被引:2,自引:2,他引:0
Kanetsuna, Fuminori (Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela), and Luis M. Carbonell. Enzymes in glycolysis and the citric acid cycle in the yeast and mycelial forms of Paracoccidioides brasiliensis. J. Bacteriol. 92:1315-1320. 1966.-Enzymatic activities in glycolysis, the hexose monophosphate shunt, and the citric acid cycle in cell-free extracts of the yeast and mycelial forms of Paracoccidioides brasiliensis were examined comparatively. Both forms have the enzymes of these pathways. Activities of glucose-6-phosphate dehydrogenase and malic dehydrogenase of the mycelial form were higher than those of the yeast form. Another 15 enzymatic activities of the mycelial form were lower than those of the yeast form. The activity of glyceraldehyde-3-phosphate dehydrogenase showed the most marked difference between the two forms, its activity in the mycelial form being about 20% of that in the yeast form. 相似文献
18.
Felipe San-Blas Gioconda San-Blas Joseph Hallak Enrique Merino 《Current microbiology》1983,8(2):85-88
Results of chemical analysis and ultrastructural study of the cell wall of a thermosensitive dimorphic mutant ofParacoccidioides brasiliensis support the involvement of α-1,3-glucan in the process of dimorphism, but do not support a possible role of this glucan as being responsible for the yeast-like morphology of this pathogenic fungus. 相似文献
19.
Pereira LA Báo SN Barbosa MS da Silva JL Felipe MS de Santana JM Mendes-Giannini MJ de Almeida Soares CM 《FEMS yeast research》2007,7(8):1381-1388
Paracoccidioides brasiliensis is an important fungal pathogen. The disease it causes, paracoccidioidomycosis (PCM), ranges from localized pulmonary infection to systemic processes that endanger the life of the patient. Paracoccidioides brasiliensis adhesion to host tissues contributes to its virulence, but we know relatively little about molecules and the molecular mechanisms governing fungal adhesion to mammalian cells. Triosephosphate isomerase (TPI: EC 5.3.1.1) of P. brasiliensis (PbTPI) is a fungal antigen characterized by microsequencing of peptides. The protein, which is predominantly expressed in the yeast parasitic phase, localizes at the cell wall and in the cytoplasmic compartment. TPI and the respective polyclonal antibody produced against this protein inhibited the interaction of P. brasiliensis to in vitro cultured epithelial cells. TPI binds preferentially to laminin, as determined by peptide inhibition assays. Collectively, these results suggest that TPI is required for interactions between P. brasiliensis and extracellular matrix molecules such as laminin and that this interaction may play an important role in the fungal adherence and invasion of host cells. 相似文献