共查询到20条相似文献,搜索用时 15 毫秒
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PCR analysis was used to create DNA markers to the Vrd1 gene. DNA of almost isogenic lines with respect to Vrd genes of the cultivars Mironovskaya 808 and Erytrospermum 604 was used. It was shown that in the monogenic Vrd1 dominant genotypes the product of amplification (280 b.p.) is absent in comparison with the DNA of the vrd recessive and monogenic Vrd2 dominant genotypes. The linkage of the marker with the Vrd1 gene has been determined using DNA analysis of plant population obtained as a result of crossing of Erytrospermum 604 (vrd recessive) and Triple Dirk C (Vrd1vrd2). F2 population segragated in two groups on the character of 280 b.p. amplification product "presence/absence". The segregation significantly coincided to the theoretical one (by ?2 test) with 1:3 expectation. The revealed molecular marker identified homozygous dominant Vrd1 plants only. The DNA-marker to Vrd1 gene is nulle-allelic 280(-). 相似文献
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RNA-binding proteins and post-transcriptional gene regulation 总被引:6,自引:0,他引:6
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A large-scale RNA in vitro selection study systematically identified RNA recognition elements for 205 RNA-binding proteins belonging to families conserved in most eukaryotes. 相似文献
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Identification and mapping of cleistogamy genes in barley 总被引:1,自引:0,他引:1
Turuspekov Y Mano Y Honda I Kawada N Watanabe Y Komatsuda T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(3):480-487
Cleistogamy is a closed type of flowering with ensured self-pollination and an important trait to study evolutionary development in flower organs, reproduction systems, gene flow, and disease control. Still, very limited information is available about the genetic control and regulatory mechanism of this trait in barley. In this work, from the eight crosses between cleistogamous and chasmogamous accessions, five crosses generated chasmogamous F1 plants and their F2 plants segregated as 3 chasmogamous:1 cleistogamous, whereas three crosses generated cleistogamous F1 plants, and their F2 plants segregated as 1 chasmogamous:3 cleistogamous. Although a single gene was responsible for the control of cleistogamy in these two groups of crosses, the direction of dominance was opposite, suggesting two genes, cly1 and Cly2, for the genetic control of cleistogamy in barley. Epistatic type of gene interaction between the two loci was detected. In the analysis of 99 recombinant inbred lines of Azumamugi × Kanto Nakate Gold and doubled haploid lines of Harrington × Mikamo Golden, where in both crosses F1 was chasmogamous, the cly1 locus has been mapped on chromosome 2HL. Using the analysis of the F2 population of Misato Golden and Satsuki Nijo where F1 was cleistogamous, the Cly2 locus was mapped in the same region of chromosome 2HL. Because the cly1 and Cly2 loci were mapped in the same region in these three different mapping populations, it was concluded that the expression of cleistogamy is under the control of two tightly linked genes or different alleles of the same gene. 相似文献
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Transcriptome reprogramming due to the introduction of a barley telosome into bread wheat affects more barley genes than wheat 下载免费PDF全文
Elodie Rey Michael Abrouk Gabriel Keeble‐Gagnère Miroslava Karafiátová Jan Vrána Sandrine Balzergue Ludivine Soubigou‐Taconnat Véronique Brunaud Marie‐Laure Martin‐Magniette Takashi R. Endo Jan Barto? International Wheat Genome Sequencing Consortium Rudi Appels Jaroslav Dole?el 《Plant biotechnology journal》2018,16(10):1767-1777
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Structure, function and regulation of the ABC1 gene product 总被引:17,自引:0,他引:17
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Cloning, restriction endonuclease mapping and post-transcriptional regulation of rpsA, the structural gene for ribosomal protein S1 总被引:17,自引:0,他引:17
Transducing lambda phages have been isolated that carry segments of the Escherichia coli chromosome in the aspC region, 20.5 min on the E. coli map. One of these phages, lambda aspC2, carries rpsA, the structural gene for the ribosomal protein S1. A three kilobase fragment from this phage, cloned into either the plasmid pACYC184 or the plasmid pBR322, was found to express S1. In cells carrying the rpsA gene on the high copy number plasmid pBR322 the rate of rpsA mRNA synthesis was increased 40-fold, whereas the rate of protein S1 synthesis was doubled, in comparison with these rates in an rpsA haploid. 相似文献
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Cris Kuhlemier 《Plant molecular biology》1992,19(1):1-14
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Karen Skriver Robert Leah Frieder Müller-Uri Finn -Lok Olsen John Mundy 《Plant molecular biology》1992,18(3):585-589
We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of Ltp1 showed that it contains an open reading frame of 351 bp interrupted by a single intron of 133 bp. Transient expression assays indicated that 702 bp of the 5 upstream region of Ltp1 is sufficient to direct aleurone-specific expression during late seed development and early germination. 相似文献
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Background and Aims
Aluminium is toxic in acid soils because the soluble Al3+ inhibits root growth. A mechanism of Al3+ tolerance discovered in many plant species involves the release of organic anions from root apices. The Al3+-activated release of citrate from the root apices of Al3+-tolerant genotypes of barley is controlled by a MATE gene named HvAACT1 that encodes a citrate transport protein located on the plasma membrane. The aim of this study was to investigate whether expressing HvAACT1 with a constitutive promoter in barley and wheat can increase citrate efflux and Al3+ tolerance of these important cereal species.Methods HvAACT1
was over-expressed in wheat (Triticum aestivum) and barley (Hordeum vulgare) using the maize ubiquitin promoter. Root apices of transgenic and control lines were analysed for HvAACT1 expression and organic acid efflux. The Al3+ tolerance of transgenic and control lines was assessed in both hydroponic solution and acid soil.Key Results and Conclusions
Increased HvAACT1 expression in both cereal species was associated with increased citrate efflux from root apices and enhanced Al3+ tolerance, thus demonstrating that biotechnology can complement traditional breeding practices to increase the Al3+ tolerance of important crop plants. 相似文献16.
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