共查询到10条相似文献,搜索用时 85 毫秒
1.
七种鲅亚科鱼Ag-NORs的比较研究 总被引:2,自引:0,他引:2
本文研究了红鳍鲅、青稍红鲅、蒙古红鲅、翘嘴红鲅、CAN条、团头鲂和长春鳊等7种鲅亚科鱼类的银染带。结果表明,在中期分裂相中各自的A g-NORs数在4-8个之间。综合分析比较国内已发表的鲤科银染资料,得出多数中国鲤科鱼类的Ag-NORs数为4位于两对sm染色体短臂端部。鲅亚科鱼在进化过程中NORs数目有增加的趋势。
Abstract:The Ag-NORs of 7 species in Abramidinae,namely Culter erythropterus,Erythroculter dabryi,E.ilishaeformis,E.mongolicus,Hemiculter leucisclus,Megalobrama amblycephala and Parabramis pekinensis were studied.The results showed that 4~8 Ag-NORs were observed in their metaphases respectively.Based on the Ag-NORs data of Cyprinid fishes published in China,we suggested that the number of Ag-NORs in major Chinese Cyprinidae is 4,locating on the short arm tips of two pairs smchromosomes.The trend of Ag-NORs number in Abramidinae fishes is towards increasing in course of evolution. 相似文献
2.
应用FISH技术鉴定一个小冰麦易位系 总被引:4,自引:0,他引:4
以生物素(biotin16dUTP)标记的天蓝冰草(Agropyronintermedium(Host)P.B.=Elytrigiaintermedia(Host)Nevski=Thinopyrumintermedium(Host)BarkworthandDewey)染色体组DNA为探针,普通小麦(TriticumaestivumL.)“中国春”DNA为封闭,用荧光原位杂交(FISH)技术对小冰麦33号进行检测。结果表明:在一对染色体的端部显现出绿色荧光,这说明小冰麦33号携带外源基因的冰草染色体片段位于小麦染色体端部,而且易位的染色体片段是较小的。从DNA水平直接证明小冰麦33是一个冰草染色体片段易位到小麦染色体端部的易位系。 相似文献
3.
HAN Fang-Pu HE Meng-Yuan BU Xiu-Ling HUANG Bai-Qu HAO Shui MA You-Zhi XIN Zhi-Yong 《植物学报(英文版)》1998,40(6)
The genomic DNA of wheatgrass (Agropyron intermedium (Host) P.B. = Elytrigia intermedia (Host) Nevski = Thinopyrum intermedium (Host) Barkworth and Dewey) was labelled with biotin-16-dUTP as a probe, and genomic DNA of common wheat ( Triticum aestivum L. ) "Chinese Spring" was used for blocking. Wheat-wheatgrass line 33 was examined by fluorescence in situ hybridization (FISH) technique. The terminal regions of a pair of chromosomes showed green fluorescent signals. It has been concluded that chromosome segrnents containing alien genes of wheatgrass are located at the terminal regions of wheat chromosomes in wheat-wheatgrass line 33, and the translocated segments were small. Wheat-wheatgrass line 33 has been proved to be a translocation line with chromosome segments of wheatgrass translocated to the terminal regions of wheat chromoSomes. 相似文献
4.
除人Y染色体外,本文采用生物素标记的人全部整条染色体特异探针与白眉长臂猿(Hylobates hoolock)有丝分裂中期分裂相进行染色体原位杂交即染色体涂染法以研究人和白眉长臂猿染色体之间的同源性。在白眉长臂猿18对常染色体上检测出了与人22对常染色体同源的59对染色体片段,确定了人和白眉长臂猿之间的精度较高的染色体连锁群。结果表明:自人与白眉长臂猿的祖先分歧以来,大量的染色体间重排(至少发生了39次易位)和染色体内的重排导致了二者核型的差异。根据杂交结果绘制了首份人和白眉长臂猿比较染色体图谱,并结合已有的人和白掌长臂猿(Hylobates lar)(2n=44)和合趾长臂猿(Hylobates syndactylus)(2n=50)的比较染色体图谱对长臂猿属的染色体进化作了初步的探讨。 相似文献
5.
Suspension cell-derived protoplasts of wheat, inactivated with different concentrations (0-2.5mol/L) of IOA, were fused by PEG method with the Haynaldia villosa protoplasts which originated from the calli 4-5d after subculture and were irradiated with 60Co-γ ray. Cell colonies, calli or regenerated plants were obtained from different combinations of fusion. The calli and plants were verified to be hybrids by chromosome counting, isozyme analysis and morphological inspection. 相似文献
6.
The cytogenetics of the backcross generations and self-bred progenies (BC2F1, BC3F1, BC2F2, BC3F2 and BC2F3) of intergenetic hybrid of Triticum aestivum L. x Eremopyrum orientale (kedeb) Jaub. Et Spach were studied. The results showed that the plants BC3F1 (2n = 43 ) isolated from the backcross generations of the plants (2n = 44 ) accounted for 41.09%, but the plants (2n = 44) isolated was only 4. 11%, and the plants BC2F2 (2n = 44) isolated from self-cross generations of the plants BC2F1 (2n = 44) accounted for 13.21%. The number of univalents in pollen mother cells was higher in some plants (BC2F1) and the averages of univalents were negatively related to the backcross seed-setting and self-cross seed-setting with a related coefficient of - 0.6766* and -0.7429* respectively. The results of genomic in situ hybridization (GISH) showed that some plants of BC2Fs (2n=44) contained different number of alien chromosomes. All those indicated that alien chromosomes caused unusual pairing and segregation of wheat homologous chromosomes and also lowed the hereditary stability of wheat chromosomes. 相似文献
7.
宁夏固原地区农村回、汉族近亲结婚的调查 总被引:1,自引:0,他引:1
胡秉平 李晓莹 何玉梅 武红梅 冯晓明 虎淑萍 余晓燕 蔺海东 孙莲 朱明宇 王桂珍 张小兰HU Bing-Ping LI Xiao-Ying HE Yu-Mei WU Hong-Mei Fen Xiao-Ming HU Shu-Ping YU Xiao-Yan LIN Hai-Dong Sun Lian ZHU Ming-Yu WANG Gui-Zeng ZHANG Xiao-Lan 《遗传》1995,17(4):14-14
8.
Comparative study of symmetric and asymmetric somatic hybridization between common wheat and Haynaldia villosa 总被引:1,自引:0,他引:1
Symmetric and asymmetric protoplast fusion between long term cell suspension-derived protoplasts of Triticum aestivum (cv. Jinan 177) and protoplasts of Haynaldia villosa prepared from one-year-old embryogeneric calli was performed by PEG method. In asymmetric fusion, donor calli were treated with gamma ray at a dose of 40, 60, 80 Gy (1.3 Gy/min) respectively and then used to isolate protoplasts. Results of morphological, cytological, biochemical (isozyme) and 5S rDNA spacer sequence analysis revealed that we obtained somatic hybrid lines at high frequency from both symmetric and asymmetric fusion. Hybrid plants were recovered from symmetric and low dose γ-fusion combinations. GISH (genomic in situ hybridization) analysis proved exactly the existence of both parental chromosomes and the common occurrence of several kinds of translocation between them in the hybrid clones regenerated from symmetric and asymmetric fusion. And the elimination of donor DNA in hybrid clones regenerated from asymmetric fusion 相似文献
9.