猕猴属6个种的rDNA变异及其系统进化关系

以人２８Ｓ,１８Ｓ,ｒＤＮＡ为探针,用１５种限制性内切酶构建了猕猴属６个种（Ｍ．ｍｕｌａｔｔａ,Ｍ．ｆａｃｓｉｃｕｌａｒｉｓｆＭ．ａｒｃｔｏｉｄｅｓ,Ｍ．ａｓｓａｍｅｎｓｉｓ．Ｍ．ｔｈｉｂｅｔａｎａ．Ｍ。ｎｅｍｅｓｔｒｉｎａ）和滇金丝猴Ｒｈｉｎｏｐｉｔｈｅｃｕｓｂｉｅｔｉ）,白颊长臂猿（Ｈｙｌｏｂａｔｅｓｌｅｕｃｏｇｅｎｙｓ）核糖体ＤＮＡ重复单位的限制性内切酶图谱,红面猴（Ｍ．ａｒｃｔｏｉｅｓ）与     

集约高产过程中土壤有机质动态初探

集约高产过程中土壤有机质动态初探崔玉亭,韩纯儒（北京农业大学,１０００９４）ＤｙｎａｍｉｃｓｏｆＳｏｉｌＯｒｇａｎｉｃＭａｔｔｅｒ（ＳＯＭ）ｉｎｔｈｅＰｒｏｃｅｓｓｏｆＡｇｒｉｃｕｌｔｕｒａｌＩｎｔｅｎｓｉｆｉｃａｔｉｏｎ￥．ＣｕｉＹｕｔｉｎｇ;ＨａｎＣｈｕｎｒｕ（ＢｅｉｊｉｎｇＡｇｒｉｃｕｌｔｕｒａｌＵｎｉｖｅｒｓｉｔｙ,１０００９４）．ＣｈｉｎｅｓｅＪｏｕｒｎａｌｏｆＥｃｏｌｏｇｙ,１９９３,１２（２）：３７—３８．Ｉｎｔｈｉｓｐａｐｅｒ,ｔｈｅｂａｌａｎｃｅｏｆｓｏｉｌｏｒｇａｎｉｃｍａｔｔｅｒ（ＳＯＭ）ｉｎｔｈｅｐｒｏｃｅｓｓｏｆａｇｒｉｃｕｌｔｕｒａｌｉｎｔｅｎｓｉｆｉｃａｔｉｏｎｉｎＣｈａｎｇｚｈｏｕｒｅｇｉｏｎｉｓｓｔｕｄｉｅｄａｎｄａｍｅｄｉｕｍ－ａｎｄｌｏｎｇ－ｔｅｒｍｐｒｅｄｉｃｔｉｏｎｏｆｉｔｓｄｙｎａｍｉｃｓｉｓｍａｄｅ。Ｔｈｅｒｅ－ｓｕｌｔｓｓｈｏｗｔｈａｔｄｕｒｉｎｇｔｈｉｓｐｒｏｃｅｓｓ,ｔｈｅｂａｌａｎｃｅｏｆＳＯＭｉｓｇｅｔｔｉｎｇｂｅｔｔｅｒ,ａｎｄａｆｔｅｒａｍｅｄｉｕｍｏｒｌｏｎｇｐｅ－ｒｉｏｄ（ｅ．ｇ,１０—２０ｙｅａｔｓ）,ｔｈｅＳＯＭｃｏｎｔｅｎｔｃａｎｇｅｔｔ     

新疆几种昼出性蝴蝶的更正（鳞翅目）

本文订正黄人鑫和村山修一（１９９２）在《蝶と蛾》（４３卷１期）上发表的几个蝴蝶新种和新亚种。１．ＰａｒｎａｓｉｕｓｐｈｏｅｂｕｓｈａｌａｓｉｃｕｓＨｕａｎｇｅｔＭｕｒａｙａｍａ,１９９２为Ｐａｒｎａｓｉｕｓｐｈｏｅｂｕｓｐｈｏｅｂｕｓ（Ｆａｂｒ．１７９３）的新异名。２．ＰａｒｎａｓｉｕｓａｒｉａｄｎｅｊｉａｄｅｎｇｙｕｅｎｓｉｓＨｕａｎｇｅｔＭｕｒａｙａｍａ,１９９２为Ｄｒｉｏｐａ（Ｅｒｙｔｈｒｏｄｉｏｐａ）ａｒｉａｄｎｅａｒｉａｄｎｅ（Ｋｉｎｄｅｒｍａｎｎ,１８５３）的新异名。３．ＰｏｎｔｉａｃａｌｉｄｉｃｅｈａｌａｓｉａＨｕａｎｇｅｔＭｕｒａｙａｍａ,１９９２为Ｓｙｎｃｈｌｏｅｃａｌｉｄｉｃｅｃａｌｉｄｉｃｅ（Ｈüｂｎｅｒ）的新异名。４．ＭｅｌｉｔａｅａｓｃｏｔｏｓｉａｗｅｉｗｕｅｒｉａＨｕａｎｇｅｔＭｕｒａｙａｍａ,１９９２为ＭｅｌｉｔａｅａｓｃｏｔｏｓｉａｂｕｔｌｅｒｉＨｉｇｇｉｎｓ的新异名。     

小麦秸秆的生化他感效应

小麦秸秆的生化他感效应马永清,毛仁钊,刘孟雨,刘小京,张玉铭（中国科学院石家庄农业现代化研究所,０５００２１）ＡｌｌｅｌｏｐａｔｈｉｃＥｆｆｅｃｔｓｏｆＷｈｅａｔＳｔｒａｗ￥ＭａＹｏｎｇｑｉｎｇ;ＭａｏＲｅｎｚｈａｏ;ＬｉｕＭｅｎｙｕ;ＬｉｕＸｉａｏｊｉｎｇ（ＳｈｉｊｉａｚｈｕａｎｇＩｎｓｔｉｔｕｔｅｏｆＡｇｒｉｃｕｌｔｕｒａｌＭｏｄｅｒｎｉｚａｔｉｏｎ,ＡｃａｄｅｍｉａＳｉｎｉｃａ,０５００２１）．ＣｈｉｎｅｓｅＪｏｕｒｎａｌｏｆＥｃｏｌｏｇｙ,１９９３,１２（５）;３６－３８。Ｔｈｅｗｏｒｌｄ－ｗｉｄｅｒｅｓｅａｒｃｈｅｓｏｎａｌｌｅｌｏｐａｔｈｉｃｅｆｆｅｃｔｓｏｆｗｈｅａｔｓｔｒａｗａｒｅｓｕｍｍａｒｉｚｅｄａｎｄｔｈｅｃｕｒｒｅｎｔｓｉｔｕａ－ｔｉｏｎｏｎｉｓｏｌａｔｉｏｎａｎｄｉｄｅｎｔｉｆｉｃａｔｉｏｎｏｆａｌｌｅｌｏｃｈｅｍｉｃａｌｓｆｒｏｍｉｔｉｓｐｒｅｓｅｎｔｅｄ．ｗｈｉｃｈｗｉｌｌｇｉｖｅａｇｕｉｄａｎｃｅｉｎｏｕｒｔｈｅｏｒｅｔｉｃａｌｓｔｕｄｙａｎｄｐｒｏｄｕｃｔｉｖｅｐｒａｃｔｉｃｅ。Ｋｅｙｗｏｒｄｓ：Ａｌｌｅｌｏｐａｔｈｙ,ａｌｌｅｌｏｃｈｅｍｉｃａｌｓ,ｗｈｅａｔｓｔｒａｗ。     

NEW SPECIES OF GENUS MANSONIELLA POPPIUS FROM CHINA (HEMIPTERA: MIRIDAE: BRYOCORINAE)

本文记述了曼盲蝽属ＭａｎｓｏｎｉｅｌａＰｏｐｐｉｕｓ,１９１５的７个新种：环曼盲蝽Ｍ．ａｎｎｕｌａｔａｓｐ．ｎｏｖ．;脊曼盲蝽Ｍ．ｃｒｉｓｔａｔａｓｐ．ｎｏｖ．;狭长曼盲蝽Ｍ．ｅｌｏｎｇａｔａｓｐ．ｎｏｖ．;黄翅曼盲蝽Ｍ．ｆｌａｖａｓｐ．ｎｏｖ．;胡桃曼盲蝽Ｍ．ｊｕｇｌａｎｄｉｓｓｐ．ｎｏｖ．;瑰环曼盲蝽Ｍ．ｒｏｓａｃｅａｓｐ．ｎｏｖ．;赤环曼盲蝽Ｍ．ｒｕｂｉｄａｓｐ．ｎｏｖ．。提出３个新组合：Ｍ．ｃｉｎｎａｍｏｍｉ（ＺｈｅｎｇｅｔＬｉｕ,１９９２）,ｃｏｍｂ．ｎｏｖ．;Ｍ．ｓａｓａｆｒｉ（ＺｈｅｎｇｅｔＬｉｕ,１９９２）,ｃｏｍｂ．ｎｏｖ．;Ｍ．ｗａｎｇｉ（ＺｈｅｎｇｅｔＬｉ,１９９２）,ｃｏｍｂ．ｎｏｖ．,均由ＰａｃｈｙｐｅｌｔｉｓＳｉｇｎｏｒｅｔ属移入本属,模式标本存放于南开大学生物系昆虫标本室     

庐山土壤微生物类群及酶活特性

庐山土壤微生物类群及酶活特性陆杨森,熊金莲,张明（安徽农学院,合肥２３００３６）ＭｉｃｒｏｂｉａｌＧｒｏｕｐｓａｎｄＥｎｚｙｍｅＡｃｔｉｖｉｔｙｉｎＳｏｉｌｏｆＬｕｓｈａｎＭｏｕｎｔａｉｎ￥ＬｕＹａｎｇｓｅｎ;ＸｉｏｎｇＪｉｎｌｉａｎ;ＺｈａｎｇＭｉｎｇ（ＡｎｈｕｉＡｇｒｉｃｕｌｔｕｒａｌＣｏｌｌｅｇｅ,Ｈｅｆｅｉ２３００３６）．ＣｈｉｎｅｓｅＪｏｕｒｎａｌｏｆＥｃｏｌｏｇｙ１９９３,１２（５）：２５－２８。ＴｈｅｔｏｔａｌｎｕｍｂｅｒｏｆｍｉｃｒｏｂｅｓｖａｒｉｅｓｉｎｄｉｆｆｅｒｅｎｔｓｏｉｌｔｙｐｅｓｏｆＬｕｓｈａｎＭｏｕｎｔａｉｎ,ｂｅｉｎｇｔｈｅｈｉｇｈｅｓｔ（１．２×１０￣９ｎｕｍｂｅｒｓｇｄｒｙｓｏｉｌ）ｉｎｍｏｕｎｔａｉｎｏｕｓｙｅｌｌｏｗｂｒｏｗｎｓｏｉｌ．Ｔｈｅｅｃｏｌｏｇｉａｌｄｉｓｔｒｉｂｕｔｉｏｎｏｆｖａｒｉｏｕｓｍｉｃｒｏｂｉａｌｇｒｏｕｐｓｉｎｄｉｆｆｅｒｅｎｔｓｏｉｌｔｙｐｅｓｉｓｓｉｍｌｌａｒ。Ｂａｃｔｅｒｉａｉｓｍｏｒｅｔｈａｎａｃｔｉｎｏｍｙｃｅｓａｎｄｆｕｎｇｉ．Ｎｏｎｉｔｒｉｆｙｉｎｇｂａｃｔｅ－ｒｉａｃａｎｂｅｆｏｕｎｄＩｎｓｏｌｌｓｏｆＬｕｓｈａｎＭｏｕｎｔａｉ     

pp60c-src在血管平滑肌细胞内丝裂原活化蛋白激酶激活中的作用

观察ｐｐ６０ｃ－ｓｒｃ在血管紧张素Ⅱ（ＡｎｇⅡ）诱导血管平滑肌细胞（ＶＳＭＣｓ）内丝裂原活化蛋白激酶（ＭＡＰＫ）激活中的作用,以了解ＡｎｇⅡ促ＶＳＭＣｓ增殖的信号转导过程。将合成的反义ｃ－ｓｒｃ寡脱氧核苷酸（ｏｌｉｇｏｄｅｏｘｙｎｕｃｌｅ－ｏｔｉｄｅｓ,ＯＤＮｓ）以脂质体包裹转染培养的大鼠ＶＳＭＣｓ,用Ｗｅｓｔｅｒｎ印迹测得细胞裂解液中ｐｐ６０ｃ－ｓｒｃ含量明显下降,免疫沉淀方法测得ｐｐ６０ｃ－ｓ     

从线粒体细胞色素b探讨长臀？属三个种分类与进化的关系

本研究测定了长臂Ｂａ属（Ｍｙｓｔａｃｏｌｅｕｃｕｓ Ｇｕｅｎｔｈｅｒ）中的３个种：月斑长臂Ｂａ（Ｍ．ｃｈｉｌｏｐｔｅｒｕｓ Ｆｏｗｌｅｒ）、长臂Ｂａ〔Ｍ．ｍａｒｇｉｎａｔｕｓ（Ｃｕｖｒｉｅｒ ｅｔ Ｖａｌｅｎｃｉｅｎｎｓｅ）〕和细尾长臂Ｂａ（Ｍ．ｌｅｐｔｕｒｕｓ Ｈｕａｎｇ）共２２个个体的线粒体细胞色素ｂ部分基因片段,其序列为４０８ｂｐ,并选用Ｐａｎｇ Ｐｉ（Ｒｈｏｄｅｕｓ ｓｉｎｅｎｓｉｓ Ｃｕ     

International Young Life Scientist Programme

Ａｔｔｈｅ１８ｔｈＩｎｔｅｒｎａｔｉｏｎａｌＣｏｎｇｒｅｓｓｏｆＢｉｏｃｈｅｍｉｓｔｒｙａｎｄＭｏｌｅｃｕｌａｒＢｉｏｌｏｇｙ,Ｂｉｒｍｉｎｇｈａｍ,ＥｎｇｌａｎｄＴｈｅＩｎｔｅｒｎａｔｉｏｎａｌＵｎｉｏｎｏｆＢｉｏｃｈｅｍｉｓｔｒｙａｎｄＭｏｌｅｃｕｌａｒＢｉｏｌｏｇｙ（ＩＵＢＭＢ）,ｔｈｅＦｅｄｅｒａｔｉｏｎｏｆＥｕｒｏｐｅａｎＢｉｏｃｈｅｍｉｃａｌＳｏｃｉｅｔｉｅｓ（ＦＥＢＳ）,ａｎｄｔｈｅＢｉｏｃｈｅｍｉｃａｌＳｏｃｉｅｔｙａｎｎｏｕｎｃｅｔｈｅｓｐｏｎｓｏｒｓｈｉｐｏｆａＳｙｍｐｏｓ…     

一种新的脉冲电泳分子量标记*

在脉冲电泳（ｐｕｌｓｅｄ－ｆｉｅｌｄ　ｇｅｌ　ｅｌｃｔｒｏｐｈｏｒｅｓｉｓ,ＰＦＧＥ）研究中,经常使用的分子量标记有啤酒酵母（Ｓａｃｃｈａｒｏｍｙｃｅｓ　ｃｅｒｅｖｅｓｌａｅ）和粟酒裂殖酵母（Ｓｃｈｉｚｏｓａｃｃｈａｒｏｍｙｃｅｓ　ｐｏｍｂｅ）的染色体完整ＤＮＡ。其中,啤酒酵母（如菌株ＹＮＮ２９５）有１６条染色体,分子量变化范围为０．２５Ｍｂ～２．２Ｍｂ（ＭｃＣｌｕｓｋｅｙｅｌａｌ,１９９０）,适于作为小于２．２Ｍｂ的染色体ＤＮＡ的分子量标记;粟酒裂殖酵母（如菌株９７２ｈ－）有３条染色体,分子量…     

六种藓类植物茎的比较解剖学研究

通过对6种藓类植物,即褶叶青藓(Brachythecium salebrosum(Web.et Mohr.)B.S.G.)、湿地匐灯藓(Plagiomnium acutum(Lindb.)Kop.)、侧枝匐灯藓(Plagiomnium maximoviczii(Lindb.)Kop.)、大凤尾藓(Fissidensnobilis Griff.)、大羽藓(Thuidium cymbifolium(Doz.et Molk.)B.S.G.)和大灰藓(Hypnum plumaeforme Wils.)嫩茎和老茎的石蜡切片和显微观察发现,同一藓类植株的嫩茎和老茎,茎结构稳定,不同种藓类植物茎横切面具有不同特征.植物体茎横切面形状、表层细胞的层数、细胞大小和细胞壁厚薄、皮层细胞大小和形状、中轴的有无以及比例等特征可以作为藓类植物的分科分类依据之一.     

真菌类遗传学分析的知识结构教学

本文以认知结构理论为指导,讨论了真菌类遗传分析与高等动植物遗传分析的内在联系,认为利用这种内在联系进行教学可收到好的效果并说明了作者的具体教学过程。 Abstract:In the paper, the relationship between genetic analysis of Fungi and genetic analysis of high animal and plant was discussed.A good results were obtained when we adopted this method in the teaching.     

Cause of Senescence of Nine Sorts of Flowers

The levels of endogenous phytohormones and respiratory rate in nine sorts of flowers such as Cymbidium faberi Rolfe, Nopalxochia ackermannii Kunth and others were investigated both at full bloom and senescence and meanwhile the effect of exogenous phytohormones on prolonging the blossoms and promoting ethylene production were tested. There is a high content of endogenous ethylene in all the long-lived flowere, about 3–16 folds higer than the short-lived ones. There is a high level of ABA at full blooming flowers of short-lived flowers, in which there is no or only some cytokinins in it, but the ratio of CTK (6BA+zeatin)/ABA is smaller(l.7). The endogenous ABA reached a much higher level at senescence in all nine sorts of flowers, so it is reasonable to consider that it is ABA which plays an important role of regulation in controlling flower's senescence. There is a much higher level of GA3 and zeatin in the long-lived flowers which is not demonstrated in the shortlived ones. The respiratory rate is one of the factors controtling the longevity of flowers, but it does not play a decided role. Application of 6BA and zeatin prolongs distinctly orchid’s longevity, however exogenous IAA through the promotive action on ethylene production, evidently extends the longevity of the flowers of the Nopalxochia ackermannii Kunth.     

Time of detection of recessive genes: effects of system of mating and number of examined individuals

Summary Anthers were cultured from two sets of seven lines of hexaploid wheat (Triticum aestivum L.) with different cytoplasms, the euplasmic nucleus donors, Siete Cerros 66 and Penjamo 62, as well as their six alloplasmic lines derived from wild relative species of the genera Triticum and Aegilops. Significant cytoplasmic and nuclear effects but no cytoplasmic-nuclear interaction were found for embryogenic anther response, with the best performance of Penjamo 62 in Ae. kotschyi cytoplasm. Plant regeneration was not affected significantly by the cytoplasmic background of the lines cultured. The possible genetic implications of the observed cytoplasmic and nuclear influences on the in vitro haploid induction of wheat are discussed.     

龙胆科药用植物化学成分的研究现状

龙胆科植物在我国的分布范围很广,且多数为药用植物,其多数种属的药用植物,至今其化学成分尚未被系统研究。综述了目前龙胆科药用植物的化学成分的研究现状及一般提取方法,对近年来发现的环烯醚萜及裂环烯醚萜类化合物进行了总结,为本科药用植物的更深入研究提供了参考。     

Scales of spatial patterns of distribution of intertidal invertebrates

Few comparative studies of spatial patterns at different scales have examined several species in the same habitat or the same species over a range of habitats. Therefore, variability in patterns among species or among habitats has seldom been documented. This study quantifies spatial patterns of a suite of intertidal snails and a species of barnacle using a range of statistical techniques. Variability in densities was quantified from the scale of adjacent quadrats (over a distance of centimeters) to tens of kilometers. Significant differences in abundances occurred primarily at two spatial scales. Small-scale differences were found at the scales of centimeters or 1–2 m and, for many species on many shores, these accounted for most of the variability in abundances from place to place. These are likely to be determined by behavioural responses to small-scale patches of microhabitat. Large-scale differences in abundance were also found in most species at the scale of hundreds of meters alongshore. These are likely to be due to variation in recruitment (and/or mortality) because of limited dispersal by adults of these species. There was little or no additional variation among shores, separated by tens of kilometers, than was shown among patches of shore separated by hundreds of meters. Identification of the scale(s) at which significant differences in abundance are found focus attention on the processes (and the scales at which these processes operate) that influence patterns of distribution and abundance. Some of the advantages and disadvantages of various procedures are discussed.     

Identification of the site of glycation of human insulin

This study evaluates the nature of glycated human insulin formed following exposure to hyperglycemic conditions in vitro. Glycated insulin was purified by RP-HPLC and its molecular mass (5971.3 Da) determined by plasma desorption mass spectrometry (MS). The difference in mass (163.7 Da) from nonglycated insulin (5807.6 Da) corresponds to a single reduced glucose (glucitol) residue. Following reduction of insulin disulfide bridges, MS confirmed that the B-chain was glycated. Enzymatic digestions with trypsin, endoproteinase Glu-C, and thermolysin, followed by RP-HPLC and identification of fragments by MS, localized glycation to the B-chain (1–5) region. Electrospray tandem MS identified the site of glycation as the B-chain NH₂-terminal Phe¹ residue. This was confirmed by automated Edman degradation with glycated human insulin.