共查询到20条相似文献,搜索用时 31 毫秒
1.
Dominique Langin 《Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids》2010,1801(3):372-376
The role of white and brown adipose tissues in energy metabolism is well established. However, the existence of brown fat in adult humans was until very recently a matter of debate, and the molecular mechanisms underlying brown adipocyte development remained largely unknown. In 2009, several studies brought direct evidence for functional brown adipose tissue in adults. New factors involved in brown fat cell differentiation have been identified. Moreover, work on the origin of fat cells took an unexpected path with the recognition of different populations of brown fat cell precursors according to the anatomical location of the fat depots: a precursor common to skeletal muscle cells and brown adipocytes from brown fat depots, and a progenitor cell common to white adipocytes and brown adipocytes that appear in certain conditions in white fat depots. There is also mounting evidence that mature white adipocytes, including human fat cells, can be converted into brown fat-like adipocytes, and that the typical fatty acid storage phenotype of white adipocyte can be altered towards a fat utilization phenotype. These data open up new opportunities for the development of drugs for obesity and its metabolic and cardiovascular complications. 相似文献
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Maria Jimenez Giorgio Barbatelli Roberta Allevi Saverio Cinti Josiane Seydoux Jean-Paul Giacobino Patrick Muzzin Frédéric Preitner 《European journal of biochemistry》2003,270(4):699-705
White and brown adipocytes are usually located in distinct depots; however, in response to cold, brown adipocytes appear in white fat. This response is mediated by beta-adrenoceptors but there is a controversy about the subtype(s) involved. In the present study, we exposed to cold beta 3-adrenoceptor knockout mice (beta 3KO) on a C57BL/6J genetic background and measured in white adipose tissue the density of multilocular cells and the expression of the brown adipocyte marker uncoupling protein-1 (UCP1). In brown fat of beta 3KO mice, UCP1 expression levels were normal at 24 degrees C as well as after a 10-day cold exposure. Strikingly, under both conditions, in the white fat of beta 3KO mice the levels of UCP1 mRNA and protein as well as the density of multilocular cells were decreased. These results indicate that beta 3-adrenoceptors play a major role in the appearance of brown adipocytes in white fat and suggest that the brown adipocytes present in white fat differ from those in brown fat. 相似文献
3.
A plasmid region encoding the active fragment and the inhibitor protein of colicin E3--CA38 总被引:7,自引:0,他引:7
Thermogenin is the purine-nucleotide binding polypeptide in brown adipose tissue mitochondria (Mr 32 000) which confers upon these mitochondria the ability to produce heat. An enzyme-linked immunosorbent assay (ELISA) has been developed to demonstrate and quantitate the occurrence of thermogenin antigen in small amounts of tissue, and thus to characterize different depots of fat tissue as white or brown. The extreme sensitivity of the method allows determination of thermogenin in samples equivalent to <1 mg tissue. The results indicate that thermogenin seems to be exclusively localised in brown fat mitochondria (as compared to white fat, liver or heart muscle mitochondria), and thermogenin antigen could only be found in brown adipocytes (as compared to white adipocytes). Thus, brown and white adipose tissue are probably ontogenetically different 相似文献
4.
Glyceride and total phosphatide levels of brown and white fat of Citellus lateralis have been followed throughout the year in biopsy samples. Extraction of the glycerides for fatty acid analysis is described. As much as 20% of the phosphatide of brown fat was found in the upper fat layer of a centrifuged tissue homogenate. This phosphatide appeared to be present as a low-density lipoprotein, and may be associated with the lipid globules of the intact cell. Glyceride and phosphatide levels varied considerably in brown fat, and no particular level was consistently observed to be associated with any part of the hibernation cycle. Fatty acid composition of the glycerides also varied widely. The degree of unsaturation was not related to the hibernation cycle, although there appeared to be a differential utilization of fatty acids during a few weeks prior to spring arousal. A decrease in tissue glyceride levels was observed in both brown and white fat during arousal from hibernation at 2°C, the loss from brown fat being double the loss from white fat. 相似文献
5.
Transcriptional control of brown fat determination by PRDM16 总被引:1,自引:0,他引:1
Seale P Kajimura S Yang W Chin S Rohas LM Uldry M Tavernier G Langin D Spiegelman BM 《Cell metabolism》2007,6(1):38-54
6.
J Wu P Boström LM Sparks L Ye JH Choi AH Giang M Khandekar KA Virtanen P Nuutila G Schaart K Huang H Tu WD van Marken Lichtenbelt J Hoeks S Enerbäck P Schrauwen BM Spiegelman 《Cell》2012,150(2):366-376
Brown fat generates heat via the mitochondrial uncoupling protein UCP1, defending against hypothermia and obesity. Recent data suggest that there are two distinct types of brown fat: classical brown fat derived from a myf-5 cellular lineage and UCP1-positive cells that emerge in white fat from a non-myf-5 lineage. Here, we report the isolation of "beige" cells from murine white fat depots. Beige cells resemble white fat cells in having extremely low basal expression of UCP1, but, like classical brown fat, they respond to cyclic AMP stimulation with high UCP1 expression and respiration rates. Beige cells have a gene expression pattern distinct from either white or brown fat and are preferentially sensitive to the polypeptide hormone irisin. Finally, we provide evidence that previously identified brown fat deposits in adult humans are composed of beige adipocytes. These data provide a foundation for studying this mammalian cell type with therapeutic potential. PAPERCLIP: 相似文献
7.
Although the functions of white fat and brown fat are increasingly well understood, their developmental origins remain unclear. A recent study published in Nature (Seale et al., 2008) identifies a population of progenitor cells that gives rise to brown fat and skeletal muscle but not white fat. 相似文献
8.
《生物化学与生物物理学报:疾病的分子基础》2014,1842(3):340-351
The obesity epidemic has intensified efforts to understand the mechanisms controlling adipose tissue development. Adipose tissue is generally classified as white adipose tissue (WAT), the major energy storing tissue, or brown adipose tissue (BAT), which mediates non-shivering thermogenesis. It is hypothesized that brite adipocytes (brown in white) may represent a third adipocyte class. The recent realization that brown fat exist in adult humans suggests increasing brown fat energy expenditure could be a therapeutic strategy to combat obesity. To understand adipose tissue development, several groups are tracing the origins of mature adipocytes back to their adult precursor and embryonic ancestors. From these studies emerged a model that brown adipocytes originate from a precursor shared with skeletal muscle that expresses Myf5-Cre, while all white adipocytes originate from a Myf5-negative precursors. While this provided a rational explanation to why BAT is more metabolically favorable than WAT, recent work indicates the situation is more complex because subsets of white adipocytes also arise from Myf5-Cre expressing precursors. Lineage tracing studies further suggest that the vasculature may provide a niche supporting both brown and white adipocyte progenitors; however, the identity of the adipocyte progenitor cell is under debate. Differences in origin between adipocytes could explain metabolic heterogeneity between depots and/or influence body fat patterning particularly in lipodystrophy disorders. Here, we discuss recent insights into adipose tissue origins highlighting lineage-tracing studies in mice, how variations in metabolism or signaling between lineages could affect body fat distribution, and the questions that remain unresolved. This article is part of a Special Issue entitled: Modulation of Adipose Tissue in Health and Disease. 相似文献
9.
Living in an enriched environment with complex physical and social stimulation leads to improved cognitive and metabolic health. In white fat, enrichment induced the upregulation of the brown fat cell fate determining gene Prdm16, brown fat-specific markers, and genes involved in thermogenesis and β-adrenergic signaling. Moreover, pockets of cells with prototypical brown fat morphology and high UCP1 levels were observed in the white fat of enriched mice associated with resistance to diet-induced obesity. Hypothalamic overexpression of BDNF reproduced the enrichment-associated activation of the brown fat gene program and lean phenotype. Inhibition of BDNF signaling by genetic knockout or dominant-negative trkB reversed this phenotype. Our genetic and pharmacologic data suggest a mechanism whereby induction of hypothalamic BDNF expression in response to environmental stimuli leads to selective sympathoneural modulation of white fat to induce "browning" and increased energy dissipation. 相似文献
10.
A new technique for single-step subcellular fractionation of adipose tissue homogenates by analytical sucrose density gradient centrifugation in a vertical pocket reorientating rotor is described. The density gradient distributions of mitochondrial and peroxisomal marker enzymes in brown and white adipose tissue of control and cold exposed rats are compared. The equilibrium density of brown fat mitochondria was found to be significantly increased compared with white fat mitochondria. GDP binding activity was localized solely to the mitochondria in both control and cold-adapted brown adipose tissue. Brown and white fat mitochondria fractions were isolated by differential centrifugation and the specific activities of various enzymes in the homogenate and mitochondrial preparations determined. The specific activity of creatine kinase in brown adipose tissue was found to be ten-fold higher than in white fat and subcellular fractionation studies showed the activity to have an exclusively cytosolic distribution in both tissues. GDP binding activity and some of the mitochondrial enzymes showed, in brown adipose, a striking increase in total activity in cold adapted rats compared to control animals. For some enzyme activities there was a small increase when expressed per mg tissue or per mg mitochondrial protein. When expressed per mg DNA i.e. per cell, there was a reduced specific activity of the mitochondrial and peroxisomal enzymes in both brown and white adipose tissue on cold adaptation. 相似文献
11.
M Néchad 《Experimental cell research》1983,149(1):119-127
The stroma of mature brown fat has been shown to contain cells which can proliferate and accumulate fat in monolayer cultures, and which have inherent characteristics distinct from those of white fat precursor cells. The purpose of the present investigation was to characterize by electron microscopic analysis these brown fat cells and their subsequent development when they were grown in vitro. By comparison with the existing ultrastructural data on brown fat in situ, it could thus be determined whether or not the precursor cells have the capacity to differentiate in culture. The stromal-vascular fraction isolated from the brown fat of weaned rats was identified as containing adipocyte stem cells, preadipocytes, endothelial cells and a few mature adipocytes. During the first week in culture (i.e., growth phase to confluence), when multilocular fat accumulation occurred, the mitochondria of the preadipocytes developed cristae and matrix granules, as they do in differentiating brown fat in situ. Such granules have been shown to be a sign of intense inner membrane synthetic activity. After confluence, the mitochondria regressed in internal structure and became morphologically more similar to white fat mitochondria. It was concluded that mature brown fat contains precursor cells which can differentiate in vitro. However, this differentiation was incomplete, and the necessity of specific factors for a full mitochondrial development in brown fat is discussed. 相似文献
12.
M J Anderson J C Rauch 《Comparative biochemistry and physiology. A, Comparative physiology》1984,79(2):305-310
Mass and gross composition of white and brown adipose tissues and of skeletal muscle were determined for C. gapperi and for M. pennsylvanicus from monthly samples of a 1-year period. Amounts of brown fat increased throughout autumn to a maximum in late winter and then declined to a minimum in the spring. Gross composition remained relatively constant throughout the year. Changes in white fat showed a trend similar to changes in brown fat. Relatively low mean values for muscle mass during summer were due to a change in the age structure of the vole population. 相似文献
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W H Harris D O Foster B E Nadeau 《Canadian journal of physiology and pharmacology》1985,63(10):1215-1220
This work was undertaken to determine if the thermogenic activity of brown fat decreased with age in young rabbits despite the morphological evidence indicating persistence of the brown adipocytes at 4 weeks of age. Data obtained by infusing five doses of noradrenaline and measuring oxygen consumption were used to construct cumulative dose-response curves for five age groups between 3 and 32 days of age. Blood flow to brown fat and other tissues was measured by the microsphere method at 1 and 3 weeks of age. The noradrenaline-induced increase in oxygen consumption when expressed as a percentage of resting oxygen consumption in millilitres per 100 g of body weight decreased (p less than 0.05) with age. However, the absolute noradrenaline-induced increase in metabolic rate (millilitres per minute) increased with age. Total blood flow to brown fat (millilitres per minute) during noradrenaline infusion was unchanged between 1 and 3 weeks of age, but when the blood flow was expressed in millilitres per minute per gram of tissue flow decreased significantly (p less than 0.05) probably because of infiltration of brown fat with white fat. These data suggest that the amount of brown fat and its thermogenic capacity remain relatively constant between 1 and 3 weeks of age, but as a thermogenic organ, brown fat becomes proportionally less effective with age because of the large increase in body mass. 相似文献
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Leptin, adiponectin, and resistin are key hormones produced by adipose tissue. In the present study, we have examined the effects of acute cold exposure (18 h at 6 degrees C) on the expression of the genes encoding these hormones in both brown and white fat of rats. Acute cold exposure resulted in a significant (p < 0.001) increase in the level of UCP1 and metallothionein-1 mRNAs in brown adipose tissue, indicative of an activation of thermogenesis. Leptin mRNA was decreased (p < 0.001) in brown fat in the cold, and there was also a small but statistically significant (p < 0.05) decrease in adiponectin mRNA; resistin mRNA did not change significantly (p > 0.05). In white fat, the level of leptin mRNA also fell in the cold (p < 0.05), but there was no significant change (p > 0.05) in either adiponectin or resistin mRNA. The serum concentration of adiponectin was unchanged following acute cold exposure. We conclude that while leptin gene expression is inhibited by exposure to cold, there is no major effect on the expression of either the adiponectin or resistin genes in white or brown fat despite the cold-induced stimulation of sympathetic activity and fatty acid flux. Thus, adiponectin and resistin are unlikely to play a key role in the extensive metabolic adaptations to cold. 相似文献
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R Bertin R Portet M Terrier 《Archives internationales de physiologie et de biochimie》1979,87(3):463-470
The variation in the amounts of cyclic AMP and cyclic GMP were studied in white and brown adipose tissues and in the liver of rats during the first week of cold exposure (5 degrees C). In white fat, only a small increase in cAMP was observed on the first day. In brown fat, parallel decreases in cAMP and cGMP contents were induced which might be related to a large mobilization of tissue fatty acids. In the liver, cold exposure barely affected the cAMP content but the level of cGMP was markedly increased. These results are discussed with regard to the respective role of these different tissues in cold-induced energetic substrate mobilization. 相似文献
20.
A comparison between the effects of cold exposure in vivo and of noradrenaline in vitro on the metabolism of the brown fat of new-born rabbits 总被引:4,自引:4,他引:0
1. Exposure of new-born rabbits to the cold leads to an increase in the incorporation of [(14)C]glucose into the glycerol of brown-fat triglyceride, but has no effect on [(14)C]glucose incorporation into triglyceride of white fat or liver. The effect of cold exposure on brown-fat triglyceride is abolished by cutting the cervical sympathetic nerve. 2. Brown fat incorporates very little [(14)C]glucose into triglyceride fatty acids, either in vivo or in vitro. 3. Noradrenaline added to incubations of brown fat from new-born rabbits stimulates O(2) consumption, CO(2) output and incorporation of glucose into triglyceride glycerol. The effects of noradrenaline in vitro are therefore consistent with the hypothesis that noradrenaline mediates the response of the brown fat of new-born rabbits to cold exposure. 4. Glycerokinase is present in the brown fat of new-born rabbits, but its activity is much less than that of the glycerokinase in the brown fat of adult rats. 5. Insulin has no effect on O(2) consumption, CO(2) output or glucose uptake in brown fat of new-born rabbits. 6. It is concluded that the thermogenic response of new-born rabbits to cold exposure is accompanied by a selective acceleration of the triglyceride cycle in brown fat. However, resynthesis of triglyceride would not account for more than 1% of the O(2) consumed in vitro by new-born rabbit brown fat in the presence of noradrenaline if respiration remains coupled to phosphorylation. 相似文献