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1.
Amy J. Horner Marc J. Weissburg Charles D. Derby 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2008,194(3):243-253
The “noses” of diverse taxa are organized into different subsystems whose functions are often not well understood. The “nose”
of decapod crustaceans is organized into two parallel pathways that originate in different populations of antennular sensilla
and project to specific neuropils in the brain—the aesthetasc/olfactory lobe pathway and the non-aesthetasc/lateral antennular
neuropil pathway. In this study, we investigated the role of these pathways in mediating shelter selection of Caribbean spiny
lobsters, Panulirus argus, in response to conspecific urine signals. We compared the behavior of ablated animals and intact controls. Our results show
that control and non-aesthetasc ablated lobsters have a significant overall preference for shelters emanating urine over control
shelters. Thus the non-aesthetasc pathway does not play a critical role in shelter selection. In contrast, spiny lobsters
with aesthetascs ablated did not show a preference for either shelter, suggesting that the aesthetasc/olfactory pathway is
important for processing social odors. Our results show a difference in the function of these dual chemosensory pathways in
responding to social cues, with the aesthetasc/olfactory lobe pathway playing a major role. We discuss our results in the
context of why the noses of many animals contain multiple parallel chemosensory systems. 相似文献
2.
Red swamp crayfish, Procambarus clarkii, presently is an important aquatic commercial species in China. The crayfish is a hot area of research focus, and its genetic improvement is quite urgent for the crayfish aquaculture in China. However, the knowledge of its genomic landscape is limited. In this study, a survey of P. clarkii genome was investigated based on Illumina’s Solexa sequencing platform. Meanwhile, its genome size was estimated using flow cytometry. Interestingly, the genome size estimated is about 8.50 Gb by flow cytometry and 1.86 Gb with genome survey sequencing. Based on the assembled genome sequences, total of 136,962 genes and 152,268 exons were predicted, and the predicted genes ranged from 150 to 12,807 bp in length. The survey sequences could help accelerate the progress of gene discovery involved in genetic diversity and evolutionary analysis, even though it could not successfully applied for estimation of P. clarkii genome size. 相似文献
3.
Light intensity preferences of Procambarus clarkii (Girard, 1852) were evaluated in an indoor fiber glass trough, consisting of five equal length (4 m) zones with light intensities
varying from dark to 1,000 lux (L), situated at the ‘Las Vegas del Guadiana’ fish farm in Extremadura (Spain). Four trials
were performed, with different light zones set in three at sections of the trough, and the fourth providing a control with
one light density (300 L) throughout the trough. Whereas the highest intensity (1,000 L) was preferred in trials 1 and 2,
the dimmest condition (D) was favored in trial 3. Crayfish showed a strong preference to both outside ends of the trough,
and the “end effect” of the trough on position of the crayfish was found to be stronger than the impact of light intensity. 相似文献
4.
Aphanomyces astaci (Saprolegniales, Oomycetes) is classified among the 100 world’s worst invasive species. This species is endemic to North
America and has been introduced into Europe by imports of their hosts, the North American crayfish species. As a consequence,
extensive mass mortalities involved several populations of the European crayfish. Here, we checked its occurrence in four
Italian populations of Procambarus clarkii, the most widespread alien crayfish in Italy. Digital image analyses and image processing techniques were used to select
micro-melanized areas in the subabdominal cuticle of 2–10 crayfish per population. All the selected areas tested positive
for A. astaci ITS nrDNA specific primers; moreover, the obtained sequences clearly corresponded to A. astaci, thus revealing that P. clarkii is an active carrier of this oomycete in Italy. Decisions are to be urgently made to control the spread of both P. clarkii and A. astaci for the conservation of the indigenous crayfish biodiversity. 相似文献
5.
This study examines the expression of the photopigment gene in the developing retina of the freshwater crayfish Procambarus clarkii(Crustacea, Malacostraca, Decapoda). Both sense and anti-sense RNA probes were used for in situ hybridization (ISH) of whole embryos collected at various stages during development. A characteristic of retinal development is the formation of screening pigment in the retinular cells of the retinal ommatidia. This pigmentation is seen as a band that begins at the lateral side of the retinal field and progresses medially. At hatching the retina is approximately 50% pigmented. ISH of whole embryos shows that expression of the photopigment gene by the retinular cells correlates with the extent of the screening pigment band in the retina and with the presence of rhabdoms within the ommatidia. Sections taken through embryos after being hybridized indicate that staining is localized in the cytoplasm of the retinular cells and in the axonal region below the basement membrane. No staining reaction was seen in the rhabdoms of older ommatidia. ISH staining was also seen at the anterior midline of the protocerebrum where extraretinal photoreceptors have been reported. The data presented here show a close correlation of opsin expression within the retinular cells of the ommatidia and the formation of the very early rhabdoms, similar to Drosophila. The results will be discussed in relation to recent studies in Drosophila that suggest rhodopsin plays a role in effecting the organization of the terminal web-like cytoskeleton at the base of the developing rhabdom microvilli. 相似文献
6.
7.
M. D. Robertson M. F. Hernandez S. R. Midway C. T. Hasler C. D. Suski 《Aquatic Ecology》2018,52(2-3):225-233
Carbon dioxide has been found to produce various negative consequences for a number of aquatic species and is projected to rise in the future for many aquatic ecosystems. Crayfish act as indicators of water quality and function as keystone species in aquatic food webs; however, there is a paucity of research on how crayfish may respond to elevated CO2. This study quantified how shelter-seeking behavior in freshwater crayfish (e.g., family Cambaridae), a behavior critical for survival and fitness, may change following exposure to elevated pCO2. Red Swamp crayfish (Procambarus clarkii, Girard, 1852) were exposed to one of three potential levels of dissolved CO2 that could be found in freshwater basins currently or under future climate change conditions: Control (100 μatm), Low (6853?±?1206 μatm), and High (19,086?±?2043 μatm) for 8 days, and were then transferred to a treatment aquarium with the same CO2 levels as the respective initial exposure. The treatment aquarium contained a shelter and was divided into three equal sections based on proximity to that shelter. Crayfish proximity to the shelter (defined by the tank sections) in the treatment aquarium was monitored every 5 s for a 2-min trial. Crayfish spent differing amounts of time in differing zones of the experiment and had different levels of activity, depending on their pCO2 exposure; crayfish acclimated to High pCO2 increased their time spent hiding and decreased their overall activity when compared to the Low pCO2 and Control treatments. Augmented shelter-seeking behavior may affect crayfish social hierarchies, feeding, mating, and mortality, which could generate cascading effects on the ecology of many freshwater ecosystems. 相似文献
8.
9.
The introduction of some crustacean species has produced alterations of freshwater environments and declines of native species worldwide. The red swamp crayfish, Procambarus clarkii Girard, was introduced in the Southwest Iberian Peninsula in the 1970’s, producing severe impacts on rice agriculture and on native biota such as macrophytes, gastropods, native crayfish and amphibians. We studied the distribution of P. clarkii in two areas of SW Iberian Peninsula: the Sado River basin (SW Portugal), an area colonized by this species around 1990, and the Parque Natural del Entorno de Doñana (SW Spain), colonized soon after its introduction in the Iberian Peninsula, in the 1970’s. Our main goal was to determine which factors limit crayfish distribution, which could help to identify the most effective management practices to contain its spread. Procambarus clarkii was found in most types of water bodies, including small and shallow ones. Distance to a crayfish source was the single predictor variable explaining crayfish occurrence in most types of habitats and in both areas. The only exception was for the Sado permanent stream points, where crayfish presence was negatively affected by an interaction between elevation and flow velocity. Other habitat characteristics have apparently little or no importance for its successful colonization. Moreover, this study indicated that overland dispersal is apparently a frequent phenomenon in this species. Our findings can be used to determine which habitats are most likely to be colonized by the crayfish and to develop practical measures which may limit its spread and minimize its impacts. 相似文献
10.
Impacts of invasive species may manifest most strongly if these organisms are highly distinct functionally from the native species they often replace. Yet, should we expect functional differences between native and invasive species of generalist organisms like freshwater crayfish? Some existing evidence has pointed to native and invasive crayfish species as ecologically equivalent. We contribute to this literature by comparing the trophic niches of the globally invasive crayfishes Pacifastacus leniusculus and Procambarus clarkii, by applying carbon and nitrogen stable isotope analyses to replicated allopatric (alone) and sympatric (together) lake populations in western Washington State, USA, where P. clarkii has been recently introduced and P. leniusculus is presumed native. Our study corrected for potential inherent differences in lake food webs as a consequence of lake abiotic or biotic characteristics using random effects in linear mixed effects models. We found that although overall trophic niche size or area of these species was not significantly different, P. leniusculus was significantly higher in trophic position than P. clarkii when also accounting for the effects of body size, sex, and lakes as random effects. This pattern of increased trophic position of P. leniusculus over P. clarkii was conserved over time in one sympatric lake for which we had data over multiple years. Cumulatively, our findings point to trophic differences between the globally cosmopolitan crayfishes P. leniusculus and P. clarkii, particularly when accounting for the ways that ecosystem context can affect food web structure of communities and the trophic resources available to these consumers. 相似文献
11.
Laura Aquiloni Sara Brusconi Elena Cecchinelli Elena Tricarico Giuseppe Mazza Annalisa Paglianti Francesca Gherardi 《Biological invasions》2010,12(11):3817-3824
The red swamp crayfish, Procambarus clarkii, is a paradigmatic invader of freshwater systems. Several attempts have been made to mitigate its multiple impacts but none
was successful. Among the different methods proposed, the use of the European eel (Anguilla anguilla) as an indigenous predator is promising but the available information about its predatory ability on crayfish is to date
scanty. To fill this gap in knowledge, we ran three experiments in wetlands and irrigation ditches in Italy. The first experiment,
in the laboratory, was aimed at quantifying the extent of predation by eels on crayfish, the second, in enclosures, the size
classes of crayfish mainly preyed and the possible effect of the eels on P. clarkii behaviour, and the third, in the field, its ability to effectively reduce crayfish populations. Results showed that eels
prey on small-sized or soft crayfish, attacking them from the back; an indirect effect was to reduce crayfish trophic activity,
which in turn might increases crayfish mortality due to starvation and decreases impact on the community. However, as shown
in the field, the use of eels should be appropriately calibrated to the context of application. Taken together, our results
show that eels might be used as a complement to the traditional trapping method. However, additional studies are necessary
to understand the adequate number of eels to be introduced and to develop appropriate methods for quantifying such effects. 相似文献
12.
Specialist predators may respond strongly to sensory cues from preferred prey, but responses by generalist predators, although
predicted to be less specific, are poorly known. Among squamate reptiles, diet and strength of response to chemical prey cues
covary geographically in snakes that are specialist predators. There have been no previous studies of correspondence between
diet and chemosensory response in lizards that are prey generalists. Actively foraging lizards discriminate between prey chemicals
and control substances. It has been speculated that differential responses among prey species are unlikely in typical species
that are dietary generalists. We examined this relationship in Podarcis
lilfordi, an omnivorous lacertid that consumes a wide variety of animal prey. In experiments in which chemical stimuli were presented
on cotton swabs, lizards responded more strongly to chemicals from a broad spectrum of prey types than to deionized water,
an odorless control. These findings plus previous data showing that P.
lilfordi is capable of prey chemical discrimination suggest that P.
lilfordi can identify a wide range of potential prey using chemical cues. However, there was no evidence of differential response
to stimuli among prey species, even in comparisons of prey included in the natural diet and potential prey not in the diet.
The results, although limited to a single species, are consistent with the hypothesis that lizard species that are prey generalists
do not exhibit the differential response strengths to chemical prey cues observed in snakes that have more specialized diets.
Received in revised form: 17 July 2001
Electronic Publication 相似文献
13.
14.
Two repeated DNA sequences isolated from a partial genomic DNA library of Helianthus annuus, p HaS13 and p HaS211, were shown to represent portions of the int gene of a Ty3 /gypsy retroelement and of the RNase-Hgene of a Ty1 /copia retroelement, respectively. Southern blotting patterns obtained by hybridizing the two probes to BglII- or DraI-digested genomic DNA from different Helianthus species showed p HaS13 and p HaS211 were parts of dispersed repeats at least 8 and 7 kb in length, respectively, that were conserved in all species studied. Comparable hybridization patterns were obtained in all species with p HaS13. By contrast, the patterns obtained by hybridizing p HaS211 clearly differentiated annual species from perennials. The frequencies of p HaS13- and p HaS211-related sequences in different species were 4.3x10(4)-1.3x10(5) copies and 9.9x10(2)-8.1x10(3) copies per picogram of DNA, respectively. The frequency of p HaS13-related sequences varied widely within annual species, while no significant difference was observed among perennial species. Conversely, the frequency variation of p HaS211-related sequences was as large within annual species as within perennials. Sequences of both families were found to be dispersed along the length of all chromosomes in all species studied. However, Ty3 /gypsy-like sequences were localized preferentially at the centromeric regions, whereas Ty1/ copia-like sequences were less represented or absent around the centromeres and plentiful at the chromosome ends. These findings suggest that the two sequence families played a role in Helianthusgenome evolution and species divergence, evolved independently in the same genomic backgrounds and in annual or perennial species, and acquired different possible functions in the host genomes. 相似文献
15.
Noel H. Holmgren 《Brittonia》2018,70(1):115-139
A revision of Penstemon sect. Saccanthera subsect. Serrulati includes a new species (P. salmonensis), a new variety (P. triphyllus var. infernalis), and the elevation of a subspecies to species (P. curtiflorus), bringing the total number of species to eight, which are keyed and described, complete with nomenclature and type citations. 相似文献
16.
Inquilines, workerless social parasites, frequently show advanced adaptations to their parasitic life style that indicate
a long co-evolutionary history with their host. Ectatomma parasiticum, the first inquiline described in the poneromorph group, usurps established colonies of E. tuberculatum and produces only sexuals. In laboratory colonies, parasites were specifically attacked by the host workers, showing a failure
in their social integration. Social interactions were frequent between parasites and their hosts, especially antennation,
interpreted as attempts to promote colonial odor transfer. Inquilines destroyed eggs laid by the other queens (67 out of 209
eggs laid), including conspecific parasites, which is unusual. Such partial integration into the host colony and potential
parasite virulence argue for a recent evolution of social parasitism in E. tuberculatum. 相似文献
17.
A genetic transformation system has been developed for callus cells of Crataegus
aronia using Agrobacterium
tumefaciens. Callus culture was established from internodal stem segments incubated on Murashige and Skoog (MS) medium supplemented with
5 mg l−1 Indole-3-butyric acid (IBA) and 0.5 mg l−1 6-benzyladenine (BA). In order to optimize the callus culture system with respect to callus growth and coloration, different
types and concentrations of plant growth regulators were tested. Results indicated that the best average fresh weight of red
colored callus was obtained on MS medium supplemented with 2 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.5 mg l−1 kinetin (Kin) (callus maintenance medium). Callus cells were co-cultivated with Agrobacterium harboring the binary plasmid pCAMBIA1302 carrying the mgfp5 and hygromycin phosphotransferase (hptII) genes conferring green fluorescent protein (GFP) activity and hygromycin resistance, respectively. Putative transgenic calli
were obtained 4 weeks after incubation of the co-cultivated explants onto maintenance medium supplemented with 50 mg l−1 hygromycin. Molecular analysis confirmed the integration of the transgenes in transformed callus. To our knowledge, this
is the first time to report an Agrobacterium-mediated transformation system in Crataegus
aronia. 相似文献
18.
V. C. Dilukshi Fernando Wesam Al Khateeb Mark F. Belmonte Dana F. Schroeder 《Plant molecular biology》2018,97(1-2):149-163
Key message
Arabidopsis det1 mutants exhibit salt and osmotic stress resistant germination. This phenotype requires HY5, ABF1, ABF3, and ABF4.Abstract
While DE-ETIOLATED 1 (DET1) is well known as a negative regulator of light development, here we describe how det1 mutants also exhibit altered responses to salt and osmotic stress, specifically salt and mannitol resistant germination. LONG HYPOCOTYL 5 (HY5) positively regulates both light and abscisic acid (ABA) signalling. We found that hy5 suppressed the det1 salt and mannitol resistant germination phenotype, thus, det1 stress resistant germination requires HY5. We then queried publically available microarray datasets to identify genes downstream of HY5 that were differentially expressed in det1 mutants. Our analysis revealed that ABA regulated genes, including ABA RESPONSIVE ELEMENT BINDING FACTOR 3 (ABF3), are downregulated in det1 seedlings. We found that ABF3 is induced by salt in wildtype seeds, while homologues ABF4 and ABF1 are repressed, and all three genes are underexpressed in det1 seeds. We then investigated the role of ABF3, ABF4, and ABF1 in det1 phenotypes. Double mutant analysis showed that abf3, abf4, and abf1 all suppress the det1 salt/osmotic stress resistant germination phenotype. In addition, abf1 suppressed det1 rapid water loss and open stomata phenotypes. Thus interactions between ABF genes contribute to det1 salt/osmotic stress response phenotypes.19.
Studying Pneumocystis has proven to be a challenge from the perspective of propagating a significant amount of the pathogen in a facile manner.
The study of several fungal pathogens has been aided by the use of invertebrate model hosts. Our efforts to infect the invertebrate
larvae Galleria
mellonella with Pneumocystis proved futile since P. murina neither caused disease nor was able to proliferate within G. mellonella. It did, however, show that the pathogen could be rapidly cleared from the host. 相似文献
20.
New combinations are proposed in anticipation of the Polygonaceae treatment in the forthcoming volume of Intermountain Flora:
Polygonum kelloggii var. esotericum, P. kelloggii var. watsonii
,
Rumex densiflorus var. pycnanthus
,
R. salicifolius var. utahensis, and R. occidentalis var. tomentellus. Typifications are proposed to facilitate ongoing studies in Polygonaceae and to maintain current usage. 相似文献