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本文应用悬滴培养、燕麦-琼脂培养等方法及显微成像技术对绒泡菌科Physaraceae 4种黏菌(黄头绒泡菌Physarum flavicomum、淡黄绒泡菌Physarum melleum、垂头绒泡菌Physarum album、针箍菌Physarella oblonga)的个体发育特征进行比较研究,首次实现了垂头绒泡菌在实验室条件下的完整生活循环。4种黏菌孢子萌发均为V-型开裂,但萌发时间有所不同,黄头绒泡菌萌发所需时间最短,仅需5h,而针箍菌萌发所需时间最长,需要2d;针箍菌和淡黄绒泡菌形成的黏变形体均可转变为游动胞,而相同条件下黄头绒泡菌和垂头绒泡菌并未有游动胞形成;4种黏菌原生质团的生长速率不同,在光照刺激下针箍菌、淡黄绒泡菌、黄头绒泡菌和垂头绒泡菌的原生质团分别经过2-3d、2-3d、7d和13d,发育成子实体;完成孢子到孢子的整个生活史,针箍菌和淡黄绒泡菌需要25-30d,黄头绒泡菌和垂头绒泡菌需要40-45d。 相似文献
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国内外学者将改良CLSI M38-A方案应用于红色毛癣菌时进行了改进。这些改进包括红色毛癣菌培养基为燕麦培养基、马铃薯葡萄糖琼脂(PDA)、沙堡弱葡萄糖琼脂(SDA),接种物仅含小分生孢子,培养基为RPMI1640,接种物量为CFU/ml,培养温度为28℃,培养时间为7 d,MICs终点确定标准为MIC-0,质控菌株选择须癣毛癣菌MRL1957和红色毛癣菌MRL666。 相似文献
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在黏菌个体发育研究的培养过程中,经常发生原质团不能转变为孢子果的情况,使个体发育停留在营养生长阶段,利用液体发酵和有饲培养相结合的方法获得的绒泡菌属黏菌原质团进行了孢子果的诱导。在饥饿条件下,通过对光照和温度的调节,获得了绒泡菌属黏菌在不同培养条件下形成孢子果和菌核的最佳条件。在固体培养基和液体培养基中获得全白绒泡菌孢子果的最佳培养条件分别为:24℃、6,000lx光照和20℃、6,000lx光照;获得扁绒泡菌孢子果的最佳培养条件分别为:26℃、6,000lx光照和20℃、6,000lx光照。淡黄绒泡菌Physarum melleum在固体培养基上既可以获得孢子果也可以获得菌核,最佳培养条件分别为:26℃、6,000lx光照和22℃、3,000lx光照;在液体培养基中只能形成菌核,条件为:22℃、6,000lx光照。 相似文献
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Axenic culture of myxomycete plasmodia has been attempted from time to time by various authors, but with very little success. From over 500 known species of myxomycetes, fewer than 20 species have been reported in axenic culture to date, including axenic myxamoebal cultures. In these cultures, the plasmodia required either complex media, or a killed bacterial supplement for growth. Furthermore, the time required for attaining the axenic state varied from several months to years. In the present study, a simple, rapid procedure has been developed to render monoxenic plasmodial cultures axenic. This procedure is based on our discovery that plasmodia have certain unusual substrate preferences that are inhibitory to the associated bacteria using Physarella oblonga as a model. The presence or absence of the bacteria could be ascertained through incubation in four different bacteriological media and by the use of a differential staining technique. 相似文献
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Growth and differentiation of plant cell cultures was increased when media were gelled with corn starch instead of agar. Dry weight of tobacco and wild carrot cell cultures on media gelled with starch was more than three times that of cultures on media gelled with agar. Higher yield of anthocyanin and dry weight of embryos were found in wild carrot cultures grown on media gelled with corn starch. The starch-mediated increase in growth and differentiation of wild carrot cells was accompanied by an increase in density of the cultures shown by higher dry weight/fresh weight ratios. 相似文献
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The plasmodial slime molds is the largest group in the phylum Amoebozoa. Its life cycle includes the plasmodial trophic stage and the spore‐bearing fruiting bodies. However, only a few species have their complete life cycle known in details so far. This study is the first reporting the morphogenesis of Didymium laxifilum and Physarum album. Spores, from field‐collected sporangia, were incubated into hanging drop cultures for viewing germination and axenic oat agar plates for viewing plasmodial development and sporulation. The spores of D. laxifilum and P. album germinated by method of V‐shape split and minute pore, respectively. The amoeboflagellates, released from spores, were observed in water film. The phaneroplasmodia of two species developed into a number of sporangia by subhypothallic type on oat agar culture. The main interspecific difference of morphogenesis was also discussed. 相似文献
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Robert G. Franke 《American journal of botany》1967,54(10):1189-1197
Double-diffusion technique was used to investigate myxomycete relationships within the order Physarales. Extracts of plasmodia of 22 slime mold isolates were reacted with five antisera produced to Plasmodia of Didymium nigripes, Physarella oblonga, Physarum polycephalum, Physarum gyrosum and Fuligo septica. Two isolates of Fuligo septica tested alike. Four isolates of Physarum pusillum did not test alike, and no valid conclusion of the relationship of this species was possible. These isolates showed strong serological affinity: (1) Physarum gyrosum, Physarella oblonga, two isolates of Fuligo septica, and possibly two isolates of Physarum pusillum, and Physarum tenerum; (2) Physarum polycephalum and Physarum flavicomum; (3) Fuligo septica and many of the species tested; (4) Didymium nigripes and at least one isolate of Didymium iridis. In most cases serologial relationships among species tested did not coincide with current taxonomy based on morphology of fructification. 相似文献
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Myxomycetes (slime molds) are unique eukaryotic microorganisms with both characteristics of fungi and amoebae. Artificial cultures grown under controlled conditions were used to study the life cycle and morphogenesis. Physarum pusillum was collected from the field. Spores were inoculated and cultured with the hanging drop method. The complete life cycle was observed from spore to spore on agar without adding any solid nutrients or bacteria as food. Life cycle morphological characteristics were described for spore germination, myxamoebae, zygote, plasmodium and sporangia formation. 相似文献