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1.

Purpose

This study presents a life cycle assessment (LCA)-based sustainable and lightweight automotive engine hood design and compares the life cycle energy consumption and potential environmental impacts of a steel (baseline) automotive engine hood with three types of lightweight design: advanced high strength steel (AHSS), aluminum, and carbon fiber.

Methods

A “cradle-to-grave” LCA including the production, use, and end-of-life stages is conducted in accordance with the ISO 14040/14044 standards. Onsite data collected by Chinese automotive companies in 2015 are used in the assessment. The Cumulative Energy Demand v1.09 method is applied to evaluate cumulative energy demand (CED), and the International Panel on Climate Change 2013 100a method is used to estimate global warming potential (GWP 100a).

Results and discussion

Among the different lightweight designs for the engine hood, the aluminum design is the most sustainable and has the lowest CED and GWP (100a) from a life cycle perspective, which is based on a lifetime driving distance of approximately 150,000 km. In addition, the AHSS design is also sustainable and lightweight. The carbon fiber design results in higher CED and GWP (100a) values than the steel (baseline) design during the life cycle but results in the largest CED and GWP (100a) savings through waste material recycling. The AHSS design exhibits the best break-even distance based on CED and GWP (100a) within 150,000 km.

Conclusions

Sensitivity analysis results show that the lifetime driving distance and material recycling rate have the largest impacts on the overall CEDs and GWPs of the three lightweight designs.
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2.

Purpose

Carbon fibers have been widely used in composite materials, such as carbon fiber-reinforced polymer (CFRP). Therefore, a considerable amount of CFRP waste has been generated. Different recycling technologies have been proposed to treat the CFRP waste and recover carbon fibers for reuse in other applications. This study aims to perform a life cycle assessment (LCA) to evaluate the environmental impacts of recycling carbon fibers from CFRP waste by steam thermolysis, which is a recycling process developed in France.

Methods

The LCA is performed by comparing a scenario where the CFRP waste is recycled by steam-thermolysis with other where the CFRP waste is directly disposed in landfill and incineration. The functional unit set for this study is 2 kg of composite. The inventory analysis is established for the different phases of the two scenarios considered in the study, such as the manufacturing phase, the recycling phase, and the end-of-life phase. The input and output flows associated with each elementary process are standardized to the functional unit. The life cycle impact assessment (LCIA) is performed using the SimaPro software and the Ecoinvent 3 database by the implementation of the CML-IA baseline LCIA method and the ILCD 2011 midpoint LCIA method.

Results and discussion

Despite that the addition of recycling phase produces non-negligible environmental impacts, the impact assessment shows that, overall, the scenario with recycling is less impactful on the environment than the scenario without recycling. The recycling of CFRP waste reduces between 25 and 30% of the impacts and requires about 25% less energy. The two LCIA methods used, CML-IA baseline and ILCD 2011 midpoint, lead to similar results, allowing the verification of the robustness and reliability of the LCIA results.

Conclusions

The recycling of composite materials with recovery of carbon fibers brings evident advantages from an environmental point of view. Although this study presents some limitations, the LCA conducted allows the evaluation of potential environmental impacts of steam thermolysis recycling process in comparison with a scenario where the composites are directly sent to final disposal. The proposed approach can be scaled up to be used in other life cycle assessments, such as in industrial scales, and furthermore to compare the steam thermolysis to other recycling processes.
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3.

Purpose

Construction and demolition (C&D) waste recycling has been considered to be a valuable option not only for minimising C&D waste streams to landfills but also for mitigating primary mineral resource depletion. However, the potentially higher cement demand due to the larger surface of the coarse recycled aggregates challenges the environmental benefits of recycling concrete. Furthermore, it is unclear how the environmental impacts depend on concrete mixture, cement type, aggregates composition and transport distances.

Methods

We therefore analysed the life cycle impacts of 12 recycled concrete (RC) mixtures with two different cement types and compared it with corresponding conventional concretes (CC) for three structural applications. The RC mixtures were selected according to laws, standards and construction practice in Switzerland. We compared the environmental impacts of ready-for-use concrete on the construction site, assuming equal lifetimes for recycled and conventional concrete in a full life cycle assessment. System expansion and substitution are considered to achieve the same functionality for all systems.

Results and discussion

The results show clear (~30 %) environmental benefits for all RC options at endpoint level (ecoindicator 99 and ecological scarcity). The difference is mainly due to the avoided burdens associated to reinforcing steel recycling and avoided disposal of C&D waste. Regarding global warming potential (GWP), the results are more balanced and primarily depend on the additional amount of cement needed for RC. Above 22 to 40 kg additional cement per cubic metre of concrete, RC exhibits a GWP comparable to CC. Additional transport distances above 15 km for the RC options do result in environmental impacts higher than those for CC.

Conclusions

In summary, the current market mixtures of recycled concrete in Switzerland show significant environmental benefits compared to conventional concrete and cause similar GWP, if additional cement and transport for RC are limited.
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4.

Purpose

End-of-life (EoL) modelling in life cycle assessment has already been broadly discussed within several studies. However, no consensus has been achieved on how to model recycling in LCA, even though several approaches have been developed. Within this paper, results arising from the application of two new EoL formulas, the product environmental footprint (PEF) and the multi-recycling-approach (MRA) ones, are compared and discussed. Both formulas consider multiple EoL scenarios such as recycling, incineration and landfill.

Methods

The PEF formula has been developed within the PEF programme whose intent is to define a harmonized methodology to evaluate the environmental performance of products. The formula is based on a 50:50 allocation approach, as burdens and benefits associated with recycling are accounted for a 50% rate. The MRA formula has been developed to change focus from products to materials. Recycling cycles and material losses over time are considered with reference to material pools. Allocation between systems is no longer needed, as the actual number of potential life cycles for a certain material is included in the calculation. Both the approaches have been tested within two case studies.

Results and discussion

Methodological differences could thereof be determined, as well as applicability concerns, due to the type of data required for each formula. As far as the environmental performance is concerned, impacts delivered by MRA are lower than those delivered by PEF for aluminium, while the opposite happens for plastic and rubber due to the higher share of energy recovery accounted in PEF formula. Stainless steel impacts are almost the same.

Conclusions and recommendations

The application of the two formulas provides some inputs for the EoL dilemma in LCA. The use of a wider perspective, better reflecting material properties all over the material life cycle, is of substantial importance to properly represent recycling situations. In MRA, such properties are treated and less data are required compared to the PEF formula. On the contrary, the PEF model better accommodates the modelling of products whose materials, at end of life, can undertake the route of recycling or recovery (or landfill), depending on country-specific EoL management practices. However, its application requires more data.
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5.

Purpose

In an effort to reduce the environmental impacts of the furniture sector, this study aimed to diagnose the environmental performance of an office cabinet throughout its life cycle.

Methods

An attributional life cycle assessment (LCA) was used, based on the ISO 14044 Standard and ILCD Handbook. The scope of the study considered the entire supply chain, from cradle to grave, including the steps of pre-manufacturing, manufacturing, use, and post-use of the product. The impact assessment method was the International Reference Life Cycle Data System (ILCD) 2011 midpoint.

Results and discussion

The results identified that the most significant environmental impact of the furniture life cycle was due to the distances covered and production of the main raw material, wood medium-density particleboard (MDP). The evaluation of transport scenarios showed environmental tradeoffs for truck fuel switches and environmental gains for the distribution of MDP from closer suppliers by truck, as well as from current supplier by truck and ship in the major categories. Furthermore, evaluation of the office cabinet post-use options showed that reuse, recycling, or energy recovery from waste cause significant environmental gains in the major categories. Wooden furniture is a potential carbon sink if its life cycle does not emit more greenhouse gases than its materials can store. The impacts of substitution scenarios varied depending on the type of product avoided.

Conclusions

The LCA proved a powerful method to diagnose and manage environmental impacts in complex product systems. The sensitivity analysis showed that it is possible to reduce the environmental impacts and, at the same time, make the furniture industry increase its economic gains and net carbon stock in the anthroposphere.
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6.

Objectives

To improve its phosphate accumulating abilities for phosphate recycling from wastewater, a magnetotactic bacterium, Magnetospirillum gryphiswaldense, was genetically modified to over-express polyphosphate kinase.

Results

Polyphosphate kinase was over-expressed in the bacterium. The recombinant strain accumulated ninefold more polyphosphate from synthetic wastewater compared to original wild type. The magnetic property of the recombinant M. gryphiswaldense strain was retained.

Conclusions

The recombinant M. gryphiswaldense can be used for phosphate removal and recovery in bioremediation.
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7.

Purpose

The aim of the current study was to analyze the impacts of acrylic fiber manufacturing on the environment and to obtain information for assisting decision makers in improving relevant environmental protection measures for green field investments in developing countries especially in Africa and Middle East and North Africa (MENA) regions. The key research questions were as follows: what are the different impacts of acrylic fiber manufacturing on the environment and which base material has the highest impact?

Methods

The life cycle assessment (LCA) started from obtaining the raw material until the end of the production process (cradle to gate analysis). Focus was given on water consumption, energy utilization in acrylic fiber production, and generated waste from the industry. The input and output data for life cycle inventory was collected from an acrylic fiber manufacturing plant in Egypt. SimaPro software was used to calculate the inventory of twelve impact categories that were taken into consideration, including global warming potential (GWP), acidification potential (AP), eutrophication potential (EP), carcinogen potential (CP), ecotoxicity potential (ETP), respiratory inorganic formation potential (RIFP), respiratory organic formation potential (ROFP), radiation potential (RP), ozone layer depletion (OLD), mineral depletion (MD), land use (LU), and fossil fuel depletion (FFD).

Results and discussion

LCA results of acrylic fiber manufacturing on the environment show that 82.0 % of the impact is on fossil fuel depletion due to the high-energy requirement for acrylonitrile production, 15.9 % of the impact is on human health, and 2.1 % on ecosystem quality. No impacts were detected on radiation potential, ozone layer depletion, land use, mineral depletion, or human respiratory system due to organic substances.

Conclusions

Based on these study results, it is concluded that acrylic fiber manufacturing is a high-energy consumption industry with the highest impact to be found on fossil fuel depletion and human health. This study is based on modeling the environmental effects of the production of 1-kg acrylic fiber and can serve to estimate impacts of similar manufacturing facilities and accordingly use these results as an indicator for better decision-making.
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8.

Introduction

It is difficult to elucidate the metabolic and regulatory factors causing lipidome perturbations.

Objectives

This work simplifies this process.

Methods

A method has been developed to query an online holistic lipid metabolic network (of 7923 metabolites) to extract the pathways that connect the input list of lipids.

Results

The output enables pathway visualisation and the querying of other databases to identify potential regulators. When used to a study a plasma lipidome dataset of polycystic ovary syndrome, 14 enzymes were identified, of which 3 are linked to ELAVL1—an mRNA stabiliser.

Conclusion

This method provides a simplified approach to identifying potential regulators causing lipid-profile perturbations.
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9.

Purpose

A set of comparative life cycle assessment case studies were undertaken to explore key issues relating to the environmental impacts of building materials. The case studies explore modeling practice for long-life components by investigating (1) recycled content and end-of-life recycling scenarios and (2) service life and maintenance scenarios. The study uses a window unit frames as the object of comparison, allowing for exploration of multiple materials and assembly techniques.

Methods

Four window frame types were compared: aluminum, wood, aluminum-clad wood, and unplasticized PVC (PVCu). These used existing product life cycle inventory data which included primary frame material, coatings, weather stripping sealants, but not glazing. The functional unit was a window frame required to produce 1 m2 of visible glazing, with similar thermal performance over a building lifespan of 80 years. The frames were compared using both the end-of-life and recycled content methods for end-of-life scenarios. The models were also tested using custom-use scenarios.

Results and discussion

Well-maintained aluminum window frames proved to be the least impactful option across all categories, in large part due to the credits delivered from recycling and expectations of long-life. Wood window frames had the least variability associated with maintenance and durability. The global warming potential (GWP) of a moderately maintained aluminum assembly was found to be 68 % less than PVCu and 50 % less than aluminum-clad wood. Using a long-life scenario, wood windows were found to have a 7 % lower GWP than the long-life scenario for aluminum-clad woods. Moderately and well-maintained aluminum windows require less energy to be produced and maintained over their lifetime than any of the wood scenarios. Expectations of service life proved to be the most important factor in considering environmental impact of frame materials.

Conclusions

The research shows significant gaps in available data—such as average realized life expectancies of common building components—while further underscoring that recycling rates are a driving factor in the environmental impact of aluminum building products. A modeling shift from the recycled content method to the end-of-life recycling method should promote goals of material recovery over pursuit of material with high recycled content. Hybrid methods, such as the use of Module D, may bridge the divide between these two approaches by providing due credit for use of recycled material, while supporting a design for recycling ethos. Further research is needed on how design and construction decisions affect collection and recovery rates in practice.
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10.

Introduction

Untargeted metabolomics is a powerful tool for biological discoveries. To analyze the complex raw data, significant advances in computational approaches have been made, yet it is not clear how exhaustive and reliable the data analysis results are.

Objectives

Assessment of the quality of raw data processing in untargeted metabolomics.

Methods

Five published untargeted metabolomics studies, were reanalyzed.

Results

Omissions of at least 50 relevant compounds from the original results as well as examples of representative mistakes were reported for each study.

Conclusion

Incomplete raw data processing shows unexplored potential of current and legacy data.
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11.

Introduction

Prostate cancer (PCa) is one of the most common malignancies in men worldwide. Serum prostate specific antigen (PSA) level has been extensively used as a biomarker to detect PCa. However, PSA is not cancer-specific and various non-malignant conditions, including benign prostatic hyperplasia (BPH), can cause a rise in PSA blood levels, thus leading to many false positive results.

Objectives

In this study, we evaluated the potential of urinary metabolomic profiling for discriminating PCa from BPH.

Methods

Urine samples from 64 PCa patients and 51 individuals diagnosed with BPH were analysed using 1H nuclear magnetic resonance (1H-NMR). Comparative analysis of urinary metabolomic profiles was carried out using multivariate and univariate statistical approaches.

Results

The urine metabolomic profile of PCa patients is characterised by increased concentrations of branched-chain amino acids (BCAA), glutamate and pseudouridine, and decreased concentrations of glycine, dimethylglycine, fumarate and 4-imidazole-acetate compared with individuals diagnosed with BPH.

Conclusion

PCa patients have a specific urinary metabolomic profile. The results of our study underscore the clinical potential of metabolomic profiling to uncover metabolic changes that could be useful to discriminate PCa from BPH in a clinical context.
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12.

Introduction

Collecting feces is easy. It offers direct outcome to endogenous and microbial metabolites.

Objectives

In a context of lack of consensus about fecal sample preparation, especially in animal species, we developed a robust protocol allowing untargeted LC-HRMS fingerprinting.

Methods

The conditions of extraction (quantity, preparation, solvents, dilutions) were investigated in bovine feces.

Results

A rapid and simple protocol involving feces extraction with methanol (1/3, M/V) followed by centrifugation and a step filtration (10 kDa) was developed.

Conclusion

The workflow generated repeatable and informative fingerprints for robust metabolome characterization.
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13.

Purpose

The improper handling of industrial hazardous waste (IHW), which comprises large amounts of toxic chemicals, heavy metals, or irradiation substances, is a considerable threat to human health and the environment. This study aims to quantify the life cycle environmental impacts of IHW landfilling and incineration in China, to identify its key factors, to improve its potential effects, and to establish a hazardous waste disposal inventory.

Methods

Life cycle assessment was conducted using the ReCiPe model to estimate the environmental impact of IHW landfilling and incineration. The characterization factors for the human toxicity and freshwater ecotoxicity categories shown in the ReCiPe were updated based on the geographies, population, food intake, and environmental conditions in China.

Results and discussion

The overall environmental burden was mainly attributed to the carcinogen category. The national carcinogen burden in 2014 at 37.8 CTUh was dominated by diesel consumption, cement and sodium hydroxide production, direct emission, transportation, and electricity generation stages caused by direct mercury and arsenic emissions, as well as indirect chromium emission. Although the atmospheric mercury emission directly caused by IHW incineration was comparative with the emission levels of developed countries, the annual direct mercury emission accounted for approximately 0.1% of the national mercury emission.

Conclusions

The key factors contributing to the reduction of the national environmental burden include the increasing diesel and electricity consumption efficiency, the reduction of cement and sodium hydroxide use, the development of air pollutant controlling systems, the reduction of transport distance between IHW disposers to suppliers, and the improvement of IHW recycling and reuse technologies.
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14.

Introduction

Oil palm (E. guineensis), the most consumed vegetable oil in the world, is affected by fatal yellowing (FY), a condition that can lead to the plant’s death. Although studies have been performed since the 1980s, including investigations of biotic and abiotic factors, FY’s cause remains unknown and efforts in researches are still necessary.

Objectives

This work aims to investigate the metabolic expression in plants affected by FY using an untargeted metabolomics approach.

Method

Metabolic fingerprinting analysis of oil palm leaves was performed using ultra high liquid chromatography–electrospray ionization–mass spectrometry (UHPLC–ESI–MS). Chemometric analysis, using principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA), was applied to data analysis. Metabolites identification was performed by high resolution mass spectrometry (HRMS), MS/MS experiments and comparison with databases and literature.

Results

Metabolomics analysis based on MS detected more than 50 metabolites in oil palm leaf samples. PCA and PLS-DS analysis provided group segregation and classification of symptomatic and non-symptomatic FY samples, with a great external validation of the results. Nine differentially expressed metabolites were identified as glycerophosphorylcholine, arginine, asparagine, apigenin 6,8-di-C-hexose, tyramine, chlorophyllide, 1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine, proline and malvidin 3-glucoside-5-(6″-malonylglucoside). Metabolic pathways and biological importance of those metabolites were assigned.

Conclusion

Nine metabolites were detected in a higher concentration in non-symptomatic FY plants. Seven are related to stress factors i.e. plant defense and nutrient absorption, which can be affected by the metabolic depression of these compounds. Two of those metabolites (glycerophosphorylcholine and 1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine) are presented as potential biomarkers, since they have no known direct relation to plant stress.
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15.

Objective

To investigate the cellulose modification process on kraft pulp during recycling by mono-endoglucanase.

Results

Pichia pastoris expressing endoglucanase, EG1, was grown in a 10 l fermenter yielding a high carboxymethyl cellulase (CMCase) activity of 340 U mg?1. EG1-mediated modification of kraft pulp resulted in a paper sheet with the tensile index and burst index increased by 10 and 6.5 %, respectively. The kink index (indicating abrupt bends in fibres) of the enzyme-treated group decreased sharply by 45 % after the first recycling, compared with a reduction of only 1 % in the control group. Furthermore, EG1 treatment decreased the growth of crystallinity from 73.5 to 73.2 % and crystal size from 7.45 to 7.21 nm, which alleviated paper aging.

Conclusion

Endoglucanase EG1 modifies the interfacial properties of fibers, which affects fibre morphology during the recycling process and improves the technical properties of the resulting pulp and paper.
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16.

Purpose

This study aims to develop a valuation scheme for environmental impacts in Thailand. This would assist in integrating environmental concerns into economic assessment tools such as cost-benefit analysis (CBA), system of environmental-economic accounting (SEEA), etc., which could be used in policymaking.

Methods

The damage categories considered are human health and ecosystem quality. The value of a quality-adjusted life year (QALY) measuring damage to human health is estimated using the budget constraint approach. The value of a biodiversity-adjusted hectare year (BAHY) measuring damage to ecosystems is calculated relatively to the value of a QALY.

Results and discussion

Potential economic production per capita, which is considered as the willingness to pay (WTP) for a QALY, is in the range 458,000–566,000 Thai baht (THB) (equivalent to 14,000–18,000 USD where 1 USD ≈ 32 THB). The value of a BAHY, which is expressed in terms of the value of a QALY, is in the range 7900–9800 THB (or around 250–300 USD), based on the exchange rate between the protection targets for ecosystems and human well-being. These results are tested by applying to the monetization of potential environmental burdens from producing several biofuels and petroleum-based fuels in Thailand; the environmental externalities account for about 20–40 % of the retail price.

Conclusions

The results can benefit policymakers in terms of decision-making on environmental management. Besides, the pathways to derive the monetary value of environmental impacts could be useful for other countries.
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17.

Background

Cord blood lipids are potential disease biomarkers. We aimed to determine if their concentrations were affected by delayed blood processing.

Method

Refrigerated cord blood from six healthy newborns was centrifuged every 12 h for 4 days. Plasma lipids were analysed by liquid chromatography/mass spectroscopy.

Results

Of 262 lipids identified, only eight varied significantly over time. These comprised three dihexosylceramides, two phosphatidylserines and two phosphatidylethanolamines whose relative concentrations increased and one sphingomyelin that decreased.

Conclusion

Delay in separation of plasma from refrigerated cord blood has minimal effect overall on the plasma lipidome.
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18.

Objectives

To improve the quality of diesel fuel via removal of aromatic compounds using Pseudomonas sp.

Results

In the present study Pseudomonas sp. was able to remove 94% of fluorene, 59% of phenanthrene, 49% of anthracene, 52% of fluoranthene, 45% of pyrene and 75% carbazole present in diesel oil. Additionally, it also does not affect the aliphatic content of fuel thus maintaining the carbon backbone of the fuel.

Conclusions

Pseudomonas sp. is a potential biocatalyst that can be used in the refining industry.
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19.

Objectives

To explore potential effects of recombinant human fibroblast growth factor 20 (rhFGF20) in the growth of cultured mouse vibrissal follicles.

Results

The growth of cultured mouse vibrissal follicles was significantly induced by rhFGF20 in a dose dependent pattern in the in vitro vibrissal follicle organ culture model. However, too high concentration of rhFGF20 could inhibit the growth of vibrissal follicles. We further demonstrated that rhFGF20 stimulated the proliferation of hair matrix cells and activated Wnt/β-catenin signaling pathway.

Conclusions

The rhFGF20 might be a potential therapeutic agent to treat hair loss disorders.
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20.

Purpose

This paper seeks ways to address positive social impacts in social life cycle assessment (SLCA) and attempts to answer two questions: How can the SLCA methodology be improved in order to systematically identify all potential positive impacts in the supply chain? How can positive impacts be taken into consideration along with negative impacts in SLCA? In order for SLCA to be an attractive tool, it needs to provide users with the possibility to include positive impacts, not as variables stipulating lack of negative impacts but rather as fulfilment of positive potentials.

Methods

By scrutinising the social impacts addressed in the SLCA UNEP/SETAC Guidelines today and reviewing approaches for positive impacts in other research fields, a developed approach to capture and aggregate positive social impacts in SLCA is proposed. To exemplify the application, the case of vehicle fuels is used to investigate the possibilities of addressing positive impacts in SLCA. This includes a literature review on potential positive social impacts linked to vehicle fuels.

Results and discussion

The subcategories in the SLCA Guidelines are proposed to be divided into positive and negative impacts and complemented with some additional positive impacts. Related indicators are proposed. A draft approach for assessing positive impacts is developed where the proposed indicators are categorised in four different levels, from low to very high potential positive impact. The possibility to aggregate positive social impacts is discussed. Besides multi-criteria decision analysis (MCDA), few useful ideas for aggregating positive impacts in SLCA were found in the literature that mostly focused on surveys and monetarisation. Positive social impacts linked to vehicle fuels (fossil fuels and biofuels) are identified, and the proposed approach is schematically applied to vehicle fuels.

Conclusions

The SLCA methodology may be refined in order to better identify and assess positive impacts, and approaches developed for capturing and aggregating such impacts are proposed. Challenges of aggregating positive and negative social impacts still remain. The knowledge on social impacts from vehicle fuels could be improved by applying the proposed approach. However, the approach needs more development to be practically applicable.
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