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1.
Safety Evaluation of Transgenic Tilapia with Accelerated Growth 总被引:2,自引:0,他引:2
Isabel Guillén Jorge Berlanga Carmen M. Valenzuela Antonio Morales José Toledo Mario P. Estrada Pedro Puentes Orlando Hayes José de la Fuente 《Marine biotechnology (New York, N.Y.)》1999,1(1):2-14
Recent advances in modern marine biotechnology have permitted the generation of new strains of economically important fish
species through the transfer of growth hormone genes. These transgenic fish strains show improved growth performance and therefore
constitute a better alternative for aquaculture programs. Recently, we have obtained a transgenic tilapia line with accelerated
growth. However, before introducing this line into Cuban aquaculture, environmental and food safety assessment was required
by national authorities. Experiments were performed to evaluate the behavior of transgenic tilapia in comparison to wild tilapia
as a way to assess the environmental impact of introducing transgenic tilapia into Cuban aquaculture. Studies were also conducted
to evaluate, according to the principle of substantial equivalence, the safety of consuming transgenic tilapia as food. Behavior
studies showed that transgenic tilapia had a lower feeding motivation and dominance status than controls. Food safety assessment
indicated that tilapia growth hormone has no biological activity when administered to nonhuman primates. Furthermore, no effects
were detected in human healthy volunteers after the consumption of transgenic tilapia. These results showed, at least under
the conditions found in Cuba, no environmental implications for the introduction of this transgenic tilapia line and the safety
in the consumption of tiGH-transgenic tilapia as an alternative feeding source for humans. These results support the culture
and consumption of these transgenic tilapia.
Received: March 9, 1998; accepted June 25, 1998. 相似文献
2.
The gene transfer technique, transgenesis, has permitted the transfer of genes from one organism to another to create new lineages of organisms with improvement in traits important to aquaculture. Genetically modified organisms (GMOs), therefore, hold promise for producing genetic improvements, such as enhanced growth rate, increased production and efficiency, disease resistance and expanded ecological ranges. The basic procedure to generate transgenic fish for aquaculture includes: (1) design and construction of transgenic DNA; (2) transfer of the gene construct into fish germ cells; (3) screening for transgenic fish; (4) determination of transgene expression and phenotype; (5) study of inheritance; and (6) selection of stable lines of transgenics.GMOs offer economic benefits, but also pose environmental threats. Optimising the mix of benefits and risks is of fundamental importance. The potential economic benefits of transgenic technology to aquaculture are obvious. Transgenic fish production has the goal of producing food for human consumption; thus the design of genetic constructs must take into consideration the potential risks to consumer health, as well as marketing strategies and product acceptance in the market. 相似文献
3.
转基因鱼的研究进展与商业化前景 总被引:3,自引:0,他引:3
转基因技术为鱼类育种开辟了新的途径。目前已培育出转生长激素基因鲤、鲑和罗非鱼,转荧光蛋白基因斑马鱼与唐鱼等可稳定遗传的转基因鱼品系,其中快长转生长激素基因鱼的获得对于提高水产养殖的产量与养殖效益具有十分重要的意义。文章简要综述了转基因鱼应用研究的成就、相关技术及生态安全方面的研究进展。显微注射仍是目前基因转植的常用方法,应用转座酶或巨核酸酶介导的转基因新技术可提高基因转植效率与整合率。转基因元件的选择应尽量考虑"全鱼"基因或"自源"基因,以减少转基因鱼食用安全方面的顾虑同时也有利于转植基因的表达与生理功效的发挥。生态安全是转基因鱼商用化面临的最大问题。虽然有研究显示转基因鱼与传统的选育鱼类相比适合度较差,但由于环境与基因型间的相互作用,根据实验室获得的转基因鱼对生态影响的结果,难以预测转基因鱼一旦逃逸会对自然水生态环境产生怎样的影响。因此应建立高度自然化的环境以获得可靠的数据客观评价生态风险,有效的物理拦截、不育化处理等生物学控制策略仍是保证转基因鱼安全应用的关键措施。 相似文献
4.
Recently, tilapia hepcidin (TH)1-5 was characterized, and its antimicrobial functions against several pathogens were reported. The antimicrobial functions of another shrimp antimicrobial peptide (AMP), chelonianin, were also characterized using a recombinant chelonianin protein (rcf) that was expressed by a stably transfected Chinese hamster ovary (CHO) cell line against pathogen infections in fish. The function of the overexpression of both AMPs in zebrafish muscles was not examined in previous studies. Herein, we investigated the antimicrobial functions of TH1-5 and chelonianin against Vibrio vulnificus (204) and Streptococcus agalactiae (SA48) in transgenic TH1-5 zebrafish and transgenic chelonianin zebrafish. The presence of TH1-5 and chelonianin enhanced the inhibitory ability in transgenic AMP zebrafish against the two different bacterial infections. The bacterial number of either V. vulnificus (204) or S. agalactiae (SA48) had decreased at 96?h after injection into transgenic AMP zebrafish muscle compared to non-transgenic zebrafish muscle. Additionally, immune-related gene expressions analyzed by real-time PCR studies showed the modulation of several genes including interleukin (IL)-10, IL-22, IL-26, MyD88, Toll-like receptor (TLR)-1, TLR-3, TLR-4, nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α, and lysozyme, and significant differences were found between transgenic AMP zebrafish and wild-type zebrafish injected with PBS at 1-24?h. These results suggest that several immune-related gene expressions were induced in transgenic TH1-5 and chelonianin zebrafish which effectively inhibited bacterial growth. The survival rate dropped to 86.6% in transgenic chelonianin zebrafish after 28 days of infection compared of the 50% survival rate in transgenic TH1-5 zebrafish after 28 days of infection. Overall, these results indicate that TH1-5 and chelonianin possess the potential to be novel candidate genes for aquaculture applications to treat fish diseases. 相似文献
5.
Expression of a Novel Piscine Growth Hormone Gene Results in Growth Enhancement in Transgenic Tilapia (Oreochromis Niloticus) 总被引:6,自引:0,他引:6
Rahman M. Azizur Mak Rohan Ayad Hala Smith Alan Maclean Norman 《Transgenic research》1998,7(5):357-370
Several lines of transgenic G1 and G2 tilapia fish (Oreochromis niloticus) have been produced following egg injection with gene constructs carrying growth hormone coding sequences of fish origin. Using a construct in which an ocean pout antifreeze promoter drives a chinook salmon growth hormone gene, dramatic growth enhancement has been demonstrated, in which the mean weight of the 7 month old G2 transgenic fish is more than three fold that of their non transgenic siblings. Somewhat surprisingly G1 fish transgenic for a construct consisting of a sockeye salmon metallothionein promoter spliced to a sockeye salmon growth hormone gene exhibited no growth enhancement, although salmon transgenic for this construct do show greatly enhanced growth. The growth enhanced transgenic lines were also strongly positive in a radio-immuno assay for the specific hormone in their serum, whereas the non growth enhanced lines were negative. Attempts to induce expression from the metallo thionein promoter by exposing fish to increased levels of zinc were also unsuccessful.Homozygous transgenic fish have been produced from the ocean pout antifreeze/chinook salmon GH construct and preliminary trials suggest that their growth performance is similar to that of the hemizygous transgenics. No abnormalities were apparent in the growth enhanced fish, although minor changes to skull shape and reduced fertility were noted in some fish. There is also preliminary evidence for improved food conversion ratios when growth enhanced transgenic tilapia are compared to their non-transgenic siblings.The long term objective of this study is to produce lines of tilapia which are both growth enhanced and sterile, so offering improved strains of this important food fish for aquaculture. 相似文献
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M. E. Herkenhoff A. O. Ribeiro J. M. Costa A. C. Oliveira M. A. D. Dias R. V. Reis Neto A. W. S. Hilsdorf D. Pinhal 《Animal genetics》2020,51(4):611-616
The Nile tilapia (Oreochromis niloticus) is a prominent farmed fish in aquaculture worldwide. Crossbreeding has recently been carried out between the Red-Stirling and the wt Chitralada strains of Nile tilapia, producing a heterotic hybrid (7/8 Chitralada and 1/8 Red-Stirling) that combines the superior growth performance of the Chitralada with the reddish coloration of the Red-Stirling strain. While classical selective breeding and crossbreeding strategies are well known, the molecular mechanisms underlying the phenotypic expression of economically advantageous traits in tilapia remain largely unknown. Molecular investigations have shown that variable expression of growth hormone (gh), insulin-like growth factors (igf1 and 2) and somatolactin (smtla) – components of the growth hormone/insulin-like growth factor (GH/IGF) axis – and myostatin (mstn) genes can affect traits of economic relevance in farmed animals. The aim of this study was to assess and compare the gene expression signature among Chitralada, Red-Stirling and their backcross hybrid in order to gain insights into the effects of introgressive breeding in modulation of the GH/IGF axis. Gene expression analyses in distinct tissues showed that most genes of the GH/IGF axis were up-regulated and mstn was down-regulated in backcross animals in comparison with Red-Stirling and Chitralada animals. These gene expression profiles revealed that backcross animals displayed a distinctive expression signature, which attests to the effectiveness of the introgressive breeding technique. Our findings also suggest that the GH/IGF axis and mstn genes might be candidate markers for fish performance and prove useful within genetic improvement programs aimed at the production of superior-quality tilapia strains using introgressive breeding. 相似文献
8.
Triploid induction offers a way of considerably reducing fertility in fish, and could therefore be employed to help ensure
that any adverse environmental impact of transgenic fish was markedly less. In order to produce sterile growth-enhanced transgenic
fish, we have induced triploidy in two lines of transgenic tilapia. Growth performance and gonadal development were analyzed
following triploidization by heat shock. Ploidy status was confirmed by nuclear size measurement of erythrocytes. Erythrocytes
of triploids were found to be 1.5 times larger than diploids. Observations of growth enhancement and gonadal development were
made on diploids and triploids from both transgenic and nontransgenic full sibling batches. In both lines, transgenic diploids
were superior in growth performance, followed by transgenic triploids, nontransgenic diploids, and nontransgenic triploids.
Although the testes of transgenic triploids were significantly smaller than those of nontransgenic triploids and nontransgenic
diploids, histologically they did not show signs of gross deformation. There were also some spermatozoa present in the testes
of some triploids, which could be indicative of reproductive functionality. However, the ovaries were devoid of oocytes, underdeveloped,
and deformed in all triploids and were completely nonfunctional. Although the best growth performance was shown by the fertile
diploid transgenics, the triploid transgenic females could offer a good option for aquaculture purposes because they showed
superior growth performance over the normal wild-type tilapias with the advantage of sterility to ensure nonhybridization
and noncontamination with the local gene pool. However, careful monitoring of potential gene flow would be required prior
to commercial use.
Received December 1, 1998; accepted May 18, 1999. 相似文献
9.
Zebrafish eggs used as bioreactors for the production of bioactive tilapia insulin-like growth factors 总被引:1,自引:0,他引:1
Shao-Yang Hu Chia-Hsuan Liao Yi-Pei Lin Yen-Hsing Li Hong-Yi Gong Gen-Hwa Lin Koichi Kawakami Tzu-Hsuan Yang Jen-Leih Wu 《Transgenic research》2011,20(1):73-83
Multiple advantages-including the short generation time, large numbers of fertilized eggs, low cost of cultivation and easy
maintenance favor the use of fish as bioreactors for the production of pharmaceutical proteins. In the present study, zebrafish
eggs were used as bioreactors to produce mature tilapia insulin-like growth factors (IGFs) proteins using the oocyte-specific
zona pellucida (zp3) promoter. The chimeric expression plasmids, pT2-ZP-tIGFs-IRES-hrGFP, in which hrGFP was used as reporter of tilapia IGFs
expression, were designed to established Tg (ZP:tIGFs:hrGFP) transgenic lines for the expression of tilapia IGF-1 and IGF-2. Recombinant tilapia IGF-1 and IGF-2 were expressed as soluble
forms in cytoplasm of fertilized eggs. The content level of tilapia IGF-1 and IGF-2 were 6.5 and 5.0% of the soluble protein,
respectively. Using a simple Ni–NTA affinity chromatography purification process, 0.58 and 0.49 mg of purified tilapia IGF-1
and IGF-2 were obtained, respectively, from 650 fertilized eggs. The biological activity of the purified tilapia IGF-1 and
IGF-2 was confirmed via a colorimetric bioassay to monitor the growth stimulation of zebrafish embryonic cells (ZF4), tilapia
ovary cells (TO-2) and human osteosarcoma epithelial cells (U2OS). These results demonstrate that the use of zebrafish eggs
as bioreactors is a promising approach for the production of biological recombinant proteins. 相似文献
10.
Martínez R Juncal J Zaldívar C Arenal A Guillén I Morera V Carrillo O Estrada M Morales A Estrada MP 《Biochemical and biophysical research communications》2000,267(1):466-472
Growth hormone (GH) has been shown to have a profound impact on fish physiology and metabolism. However, detailed studies in transgenic fish have not been conducted. We have characterized the food conversion efficiency, protein profile, and biochemical correlates of growth rate in transgenic tilapia expressing the tilapia GH cDNA under the control of human cytomegalovirus regulatory sequences. Transgenic tilapia exhibited about 3.6-fold less food consumption than nontransgenic controls (P < 0.001). The food conversion efficiency was significantly (P < 0.05) higher (290%) in transgenic tilapia (2.3 +/- 0.4) than in the control group (0.8 +/- 0.2). Efficiency of growth, synthesis retention, anabolic stimulation, and average protein synthesis were higher in transgenic than in nontransgenic tilapia. Distinctive metabolic differences were found in transgenic juvenile tilapia. We had found differences in hepatic glucose, and in agreement with previous results we observed differences in the level of enzymatic activities in target organs. We conclude that GH-transgenic juvenile tilapia show altered physiological and metabolic conditions and are biologically more efficient. 相似文献
11.
A. H. Rengmark A. Slettan † W. J. Lee ‡ Ø. Lie ‡ F. Lingaas § 《Journal of fish biology》2007,71(SC):409-422
This paper describes the finding of genes displaying differential expression in Nile tilapia ( Oreochromis niloticus ) adapted to saltwater and discusses their potential involvement in saltwater tolerance. The availability of fresh water is severely limited in many countries; therefore, the use of brackish-water areas may present an opportunity to expand the tilapia aquaculture industry. Increased knowledge of genes involved in saltwater tolerance will facilitate selection for this trait in tilapia. A set of fish from four different families of Nile tilapia was divided into two freshwater containers, the different families equally represented in the two experimental groups, and salinity was gradually increased in one of them. A differential expression study identified a number of genes expressed differently in saltwater and fresh water. Based on these expression analyses and on a judgement of potential candidate genes probably to be involved in adaptation to changes in salinity, four genes were selected and their levels of expression further analysed by real-time polymerase chain reaction. The selected genes beta haemoglobin , Ca2+ transporting plasma membrane ATPase , pro-opiomelanocortin and beta-actin showed changes in expression. The genes were mapped and assigned to an already existing, high-resolution linkage map. 相似文献
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The paper emphasizes on the several attempts made to raise the brackish water tilapia species, Tilapia guineensis both on an experimental and production basis by researchers and fish farmers in Nigeria. Besides, the aquaculture potentials
of Tilapia guineensis have been reported by several authors. However, problems exist which are not found with the culture of other tilapia species.
Even under most favourable conditions (e.g. the monosex culture of Tilapia guineensis), a poor growth rate and a mediocre feed conversion do not presage profitable aquaculture exploitation. The present review
therefore throws light on areas of further research to enhance the growth performance of Tilapia guineensis with emphasis on fish larvae nutrition and first feeding (development of bio-encapsulated feed for larval fish based on nutritionally
enriched nematodes and Calanoid copepods), digestibibilty of the feed ingredients, elucidation of the dietary protein requirements,
improved culture technique through the use of the Recirculating Aquaculture Systems and the adoption of the most recent technology
(The YY Male technology) that produces Genetically Male Tilapia through genetic manipulation. Specific recommended areas for
further research are also proffered. 相似文献
15.
Growth and nutritional trials on transgenic Nile tilapia containing an exogenous fish growth hormone gene 总被引:4,自引:1,他引:3
M. A. Rahman A. Ronyai B. Z. Engidaw K. Jauncey G-L. Hwang A. Smith E. Roderick D. Penman L. Varadi N. Maclean 《Journal of fish biology》2001,59(1):62-78
In a long-term growth trial, transgenic tilapia Oreochromis niloticus L. showed a 2·5-fold increase in growth compared with non-transgenic siblings. At 7 months, mean mass of transgenic tilapia was 653 g compared with 260 g for non-transgenic siblings. A significant increase ( P >0·01) in head: total length ratio, viscera-somatic index and hepato-somatic index was observed in transgenic fish. Female gonado-somatic index ( I G) was found to be lower in transgenics than non-transgenic siblings in both mixed and separate culture conditions. Transgenic male I G values were found to be higher in mixed culture and lower in separate culture than that of their non-transgenic siblings. Food conversion efficiency was more than 20% greater in the transgenic fish. In a second shorter-term growth performance trial, the transgenic fish grew to about four times the size of their non-transgenic siblings. A digestibility trial suggested that transgenic tilapia were more efficient utilizers of protein, dry matter and energy. Apparent digestibility of protein and apparent energy digestibility were higher in the transgenic fish. 相似文献
16.
A co-injection strategy was employed to improve the efficiency of integration of a poorly integrating but commercially important growth hormone gene construct in tilapia. Its co-injection with a reporter gene construct of higher integration efficiency yielded a threefold increase in the integration efficiency of the growth hormone gene construct. In addition, out of 13 transgenic founder tilapia generated, three transmitted the transgenes to G1 and G2 progeny with expected Mendelian inheritance ratios in the G2 generation. We also observed expression of both constructs in a number of founder and G1/G2 individuals. Evidence is provided for the co-ligation of the two constructs and we suggest that this accounts for the increased integration efficiency of growth hormone gene construct and its successful transmission and expression, thus generating lines of novel growth hormone expressing tilapia 相似文献
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Transgene activity following somatic transgenesis in Nile tilapia Oreochromis niloticus 总被引:1,自引:1,他引:0
A detailed study was made of the persistence and expression of a plasmid-enclosed reporter gene construct after intramuscular injection into the somatic muscle tissue of juvenile Nile tilapia Oreochromis niloticus and also the effect of injecting a potentially growth-promoting gene construct. The plasmid-enclosed DNA proved stable at the site of injection, lasting in some cases for up to 6 months, and was, at a very low frequency, detected in gonad tissue, indicating occasional substantial movement from the injected muscle site. It was observed that the reporter gene and regulatory sequences were also functional within the somatic cells. In a comparison of expression levels by direct somatic injection, the 1·6 kb tilapia β-actin regulatory sequence (tiβAP) resulted in c. three-fold higher β-galactosidase activity than the 4·7 kb carp β-actin regulatory sequence (cβAP) when spliced to the lacZ gene. The enhancer element near the end of first intron in the tiβAP, when co-injected with tiβAP/lacZ plasmid at a 3:1 ratio, drove significantly higher reporter activity in somatic cells than the tiβAP/lacZ sequence alone. The introduction of a growth-promoting construct, the Nile tilapia growth hormone gene driven by a tiβAP, yielded no detectable growth enhancement. 相似文献
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Selective breeding of tilapia populations started in the early 1990s and over the past three decades tilapia has become one of the most important farmed freshwater species, being produced in more than 125 countries around the globe. Although genome assemblies have been available since 2011, most of the tilapia industry still depends on classical selection techniques using mass spawning or pedigree information to select for growth traits with reported genetic gains of up to 20% per generation. The involvement of international breeding companies and research institutions has resulted in the rapid development and application of genomic resources in the last few years. GWAS and genomic selection are expected to contribute to uncovering the genetic variants involved in economically relevant traits and increasing the genetic gain in selective breeding programs, respectively. Developments over the next few years will probably focus on achieving a deep understanding of genetic architecture of complex traits, as well as accelerating genetic progress in the selection for growth-, quality- and robustness-related traits. Novel phenotyping technologies (i.e. phenomics), lower-cost whole-genome sequencing approaches, functional genomics and gene editing tools will be crucial in future developments for the improvement of tilapia aquaculture. 相似文献