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1.
Malou N Tran TN Nappez C Signoli M Le Forestier C Castex D Drancourt M Raoult D 《PloS one》2012,7(2):e31744
Background
The cause of past plague pandemics was controversial but several research teams used PCR techniques and dental pulp as the primary material to reveal that they were caused by Yersinia pestis. However, the degradation of DNA limits the ability to detect ancient infections.Methods
We used for the first time immuno-PCR to detect Yersinia pestis antigens; it can detect protein concentrations 70 times lower than the standard ELISA. After determining the cut-off value, we tested 34 teeth that were obtained from mass graves of plague, and compared previous PCR results with ELISA and immuno-PCR results.Results
The immuno-PCR technique was the most sensitive (14 out of 34) followed by the PCR technique (10 out of 34) and ELISA (3 out of 34). The combination of these three methods identified 18 out of 34 (53%) teeth as presumably being from people with the plague.Conclusion
Immuno-PCR is specific (no false-positive samples were found) and more sensitive than the currently used method to detect antigens of ancient infections in dental pulp. The combination of three methods, ELISA, PCR and immuno-PCR, increased the capacity to identify ancient pathogens in dental pulp. 相似文献2.
To explore further the diversity of the microorganisms and their relationship with geothermal sinters, we examined the lipids preserved in six sinters associated with four different hot spring (58-82 degrees C) areas of the Taupo Volcanic Zone (TVZ), New Zealand. These sinters contain microbial remains, but the process of mineralization has rendered them largely unidentifiable. Dominant lipids include free fatty acids, 1,2-diacylglycerophospholipids, 1,2-di-O-alkylglycerols, glycerol dialkylglycerol tetraethers and 1-O-alkylglycerols. These confirm the presence and, in some cases, high abundances of bacteria in all six sinters and archaea in four of the six sinter samples; in addition, the presence of novel macrocyclic diethers and unusual distributions of monoethers and diethers suggest the presence of previously uncharacterized bacteria. The lipid distributions are also markedly dissimilar among the four sites; for example, novel macrocyclic diethers are restricted to the Rotokawa samples while particularly abundant monoethers occur only in the Orakei Korako sample. Thus, biomarkers can provide crucial insight into the complex community structure of thermophilic microorganisms, including both archaea and bacteria, involved with biogenic silica sinter formation. 相似文献
3.
The detection of ancient microbial DNA offers a new approach for the study of infectious diseases, their occurrence, frequency and host-pathogen interaction in historic times and populations. Moreover, data obtained from skeletal and mummified tissue may represent an important completion of contemporary phylogenetic analyses of pathogens. In the last few years, a variety of bacterial, protozoal and viral infections have been detected in ancient tissue samples by amplification and characterization of specific DNA fragments. This holds particularly true for the identification of the Mycobacterium tuberculosis complex, which seems to be more robust than other microbes due to its waxy, hydrophobic and lipid-rich cell wall. These observations provided useful information about the occurrence, but also the frequency of tuberculosis in former populations. Moreover, these studies suggest new evolutionary models and indicate the route of transmission between human and animals. Until now, other pathogens, such as Mycobacterium leprae, Yersinia pestis, Plasmodium falciparum and others, have occasionally been identified - mostly in single case studies or small sample sizes - as well, although much less information is available on these pathogens in ancient settings. The main reason therefore seems to be the degradation and modification of ancient DNA by progressive oxidative damage. Furthermore, the constant risk of contamination by recent DNA forces to take time and cost effective measures and renders the analysis of ancient microbes difficult. Nevertheless, the study of microbial ancient DNA significantly contributes to the understanding of transmission and spread of infectious diseases, and potentially to the evolution and phylogenetic pathways of pathogens. 相似文献
4.
Recently, microbiologists have established the existence of biogeographic patterns among a wide range of microorganisms. The focus of the field is now shifting to identifying the mechanisms that shape these patterns. Here, we propose that four processes - selection, drift, dispersal and mutation - create and maintain microbial biogeographic patterns on inseparable ecological and evolutionary scales. We consider how the interplay of these processes affects one biogeographic pattern, the distance-decay relationship, and review evidence from the published literature for the processes driving this pattern in microorganisms. Given the limitations of inferring processes from biogeographic patterns, we suggest that studies should focus on directly testing the underlying processes. 相似文献
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Rollo F Marota I 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》1999,354(1379):111-119
The analysis of the DNA of ancient micro-organisms in archaeological and palaeontological human remains can contribute to the understanding of issues as different as the spreading of a new disease, a mummification process or the effect of diets on historical human populations. The quest for this type of DNA, however, can represent a particularly demanding task. This is mainly due to the abundance and diffusion of bacteria, fungi, yeasts, algae and protozoans in the most diverse environments of the present-day biosphere and the resulting difficulty in distinguishing between ancient and modern DNA. Nevertheless, at least under some special circumstances, by using rigorous protocols, which include an archaeometric survey of the specimens and evaluation of the palaeoecological consistency of the results of DNA sequence analysis, glimpses of the composition of the original microbial flora (e.g. colonic flora) can be caught in ancient human remains. Potentials and pitfalls of this research field are illustrated by the results of research works performed on prehistoric, pre-Columbian and Renaissance human mummies. 相似文献
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10.
Assessing ancient DNA studies 总被引:17,自引:0,他引:17
The study of ancient DNA has the potential to make significant and unique contributions to ecology and evolution. However, the techniques used contain inherent problems, particularly with regards to the generation of authentic and useful data. The solution currently advocated to reduce contamination and artefactual results is to adopt criteria for authentication. Nevertheless, these criteria are not foolproof, and we believe that they have, in practice, replaced the use of thought and prudence when designing and executing ancient DNA studies. We argue here that researchers in this field must take a more cognitive and self-critical approach. Specifically, in place of checking criteria off lists, researchers must explain, in sufficient enough detail to dispel doubt, how the data were obtained, and why they should be believed to be authentic. 相似文献
11.
Robert-Jan den Tex Jesus E. Maldonado Richard Thorington Jennifer A. Leonard 《Genetica》2010,138(9-10):979-984
The application of ancient DNA techniques is subject to many problems caused primarily by low quality and by low quantity of DNA. For these reasons most studies employing ancient DNA rely on the characterization of mitochondrial DNA, which is present in many more copies per cell than nuclear DNA and hence more copies are likely to survive. We used universal and taxon specific mitochondrial primers to amplify DNA from museum specimens, and found many instances where the amplification of nuclear copies of the mitochondrial gene (numts) instead of the targeted mitochondrial fragment had occurred. Furthermore, the likelihood of amplifying numts increased dramatically when universal primers were utilized. Here we suggest that ancient DNA practitioners must consider the possibility that numts can be amplified at higher rates than previously thought. This is another complication for ancient DNA studies, but it also suggests that more extensive inclusion of nuclear markers in ancient DNA studies should be feasible. 相似文献
12.
Beyond host-pathogen interactions: microbial defense strategy in the host environment 总被引:1,自引:0,他引:1
Fialho AM Stevens FJ Das Gupta TK Chakrabarty AM 《Current opinion in biotechnology》2007,18(3):279-286
Many extracellular pathogenic bacteria colonize human or animal bodies through evasion of the host immune system, a process called host-pathogen interaction. What happens when other intruders try to invade the same host and try to establish themselves in the same niche is largely unknown. In one well-studied case, Pseudomonas aeruginosa is known to secrete the protein azurin as a weapon against such invaders as cancers, parasites and viruses. The production of such weapons by pathogenic bacteria could provide important insights into how a pathogen responds in the post-colonization state to impede other intruders for its own survival. Moreover, these molecules might find use in the pharmaceutical industry as next-generation therapeutics. 相似文献
13.
The survival of ancient DNA in specimens up to several thousands of years old is established. However, there have been several claims concerning the recovery of geologically ancient DNA from fossil material many millions of years old. The authenticity of these fossil DNA sequences is questionable on theoretical and empirical grounds, and the existence of authentic geologically ancient DNA remains to be proven. 相似文献
14.
We have sequenced and analyzed 8.3 kb of sequence adjacent and distal to the human ribosomal DNA (rDNA); this distal sequence
connects to the rDNA cluster just 4 kb upstream of the first promoter and is shared among the acrocentric chromosomes and,
at least in part, it is also present in other primates. The sequence differs in character from that of the rDNA intergenic
spacer (IGS) in that it does not contain long stretches of either polypyrimidine or polypurine. However, just like the IGS,
it contains numerous repetitive elements, including retroposed fragments of 28S rRNA and large pieces of the IGS. In addition,
we show that the rDNA clusters are not interrupted by other sequences and do not recombine with this distal segment.
Received: 9 September 1996; in revised form: 4 February 1997 / Accepted: 24 February 1997 相似文献
15.
We demonstrate quantitatively that, as predicted by evolutionary theory, sequences of homologous proteins from different species converge as we go further and further back in time. The converse, a non-evolutionary model can be expressed as probabilities, and the test works for chloroplast, nuclear and mitochondrial sequences, as well as for sequences that diverged at different time depths. Even on our conservative test, the probability that chance could produce the observed levels of ancestral convergence for just one of the eight datasets of 51 proteins is ≈1×10−19 and combined over 8 datasets is ≈1×10−132. By comparison, there are about 1080 protons in the universe, hence the probability that the sequences could have been produced by a process involving unrelated ancestral sequences is about 1050 lower than picking, among all protons, the same proton at random twice in a row. A non-evolutionary control model shows no convergence, and only a small number of parameters are required to account for the observations. It is time that that researchers insisted that doubters put up testable alternatives to evolution. 相似文献
16.
Contrary to the generally held notions about microbial survival, the recently published paper by Johnson et al., 'Ancient bacteria show evidence of DNA repair', presents evidence suggesting that non-spore-forming bacteria in ancient samples are apparently alive, as judged by intact DNA, and fare better than spores. The data presented in this work raise intriguing questions about the nature of bacteria in many of the ancient samples reported to date: are they spores, persisters, sessile vegetative cells or do they make up a slow-growing population? 相似文献
17.
Römpler H Dear PH Krause J Meyer M Rohland N Schöneberg T Spriggs H Stiller M Hofreiter M 《Nature protocols》2006,1(2):720-728
This method is designed to assemble long, continuous DNA sequences using minimal amounts of fragmented ancient DNA as template. This is achieved by a two-step approach. In the first step, multiple fragments are simultaneously amplified in a single multiplex reaction. Subsequently, each of the generated fragments is amplified individually using a single primer pair, in a standard simplex (monoplex) PCR. The ability to amplify multiple fragments simultaneously in the first step allows the generation of large amounts of sequence from rare template DNA, whereas the second nested step increases specificity and decreases amplification of contaminating DNA. In contrast to current protocols using many template-consuming simplex PCRs, the method described allows amplification of several kilobases of sequence in just one reaction. It thus combines optimal template usage with a high specificity and can be performed within a day. 相似文献
18.
Bardgett RD Richter A Bol R Garnett MH Bäumler R Xu X Lopez-Capel E Manning DA Hobbs PJ Hartley IR Wanek W 《Biology letters》2007,3(5):487-490
When glaciers retreat they expose barren substrates that become colonized by organisms, beginning the process of primary succession. Recent studies reveal that heterotrophic microbial communities occur in newly exposed glacial substrates before autotrophic succession begins. This raises questions about how heterotrophic microbial communities function in the absence of carbon inputs from autotrophs. We measured patterns of soil organic matter development and changes in microbial community composition and carbon use along a 150-year chronosequence of a retreating glacier in the Austrian Alps. We found that soil microbial communities of recently deglaciated terrain differed markedly from those of later successional stages, being of lower biomass and higher abundance of bacteria relative to fungi. Moreover, we found that these initial microbial communities used ancient and recalcitrant carbon as an energy source, along with modern carbon. Only after more than 50 years of organic matter accumulation did the soil microbial community change to one supported primarily by modern carbon, most likely from recent plant production. Our findings suggest the existence of an initial stage of heterotrophic microbial community development that precedes autotrophic community assembly and is sustained, in part, by ancient carbon. 相似文献
19.
We have shown, in a previous paper, that tandem repeating sequences, especially triplet repeats, play a very important role in gene evolution. This result led to the formulation of the following hypothesis: most of the genomic sequences evolved through everlasting acts of tandem repeat expansions with subsequent accumulation of changes. In order to estimate how much of the observed sequences have the repeat origin we describe the adaptation of a text segmentation algorithm, based on dynamic programming, to the mapping of the ancient expansion events. The algorithm maximizes the segmentation cost, calculated as the similarity of obtained fragments to the putative repeat sequence. In the first application of the algorithm to segmentations of genomic sequences, a significant difference between the natural sequences and the corresponding shuffled sequences is detected. The natural fragments are longer and more similar to the putative repeat sequences. As our analysis shows, the coding sequences allow for repeats only when the size of the repeated words is divisible by three. In contrast, in the non-coding sequences, all repeated word sizes are present. It was estimated, that in Escherichia coli K12 genome, about 35.5% of sequence can be detectably traced to original simple repeat ancestors. The results shed light on the genomic sequence organization, and strongly confirm the hypothesis about the crucial role of triplet expansions in gene origin and evolution. 相似文献
20.
P. Francalacci 《Human Evolution》1995,10(1):81-91
The recovery, amplification and sequencing of nucleic acids from ancient smaples opens new possibilities in many different fields, such as anthropology, archeaology, population genetics, animal and plant evolutionary studies, and forensic medicine. The sample processing for DNA extraction and PCR amplification represents the most delicate phase of ancient DNA analysis, with a major impact on the reproducibility and reliability of the results. In this paper some extraction protocols are reviewed and discussed, with particular reference to the removal of the inhibitory substances usually present in extract from ancient tissues. The effect of contamination from extraneous DNA, a possible source of misleading results, is discussed and guidelines to detect and circumvent the problem are given. 相似文献