共查询到20条相似文献,搜索用时 625 毫秒
1.
2.
Tu JM Kupka M Böhm S Plöscher M Eichacker L Zhao KH Scheer H 《Photosynthesis research》2008,95(2-3):163-168
The phycobilin: Cysteine-84-phycobiliprotein lyase, CpeS1, catalyzes phycocyanobilin (PCB) and phycoerythrobilin attachment
to nearly all cysteine-84 (consensus sequence) binding sites of phycoerythrin, phycoerythrocyanin, phycocyanin and allophycocyanin
(Zhao et al. (2007) Proc Natl Acad Sci 104:14300–14305). We now show that CpeS1 can bind PCB, as assayed by Ni2+ chelating affinity chromatography. Binding is rapid, and the chromophore is bound in an extended conformation similar to
that in phycobiliproteins but only poorly fluorescent. Upon addition of apo-biliproteins, the chromophore is transferred to
the latter much slower (∼1 h), indicating that chromophorylated CpeS1 is an intermediate in the enzymatic reaction. In addition,
imidazole is bound to PCB, as shown by mass spectroscopy of tryptic digests of the intermediate CpeS1–PCB complex. 相似文献
3.
4.
K. Tukamoto 《Journal of fish biology》1990,36(5):659-671
A total of 149 Anguilla japonica elvers collected at 10 locations along the Japanese coast were aged according to otolith daily increments. The interrelationships among age, birth date, body size, pigmentation stage, sampling location and the timing of recruitment were examined in order to determine the recruitment mechanism of elvers to coastal waters.
First catches of eels were earlier in the locations at lower latitudes. Age at recruitment was roughly constant, 218 ± 29 (mean ± s.d.) days old, disregarding the localities, but a weak positive correlation was obtained between age and sampling date or timing of recruitment. Body length at recruitment was also constant, 56.3 ± 2.3 mm s.l., and showed no significant correlation with either locality or recruitment timing. Estimated birth date ranged from April to November, the mean ± s.d. being 22 July 1982 ± 42 days, suggesting a peak season spawning in summer. Birth date was closely related with both the latitude of location and sampling time. Pigmentation developed more at lower latitude. Recruitment mechanism of the Japanese eel was summarized as follows: the earlier-born individual recruits earlier at lower latitude and at younger age, but at a constant body size. 相似文献
First catches of eels were earlier in the locations at lower latitudes. Age at recruitment was roughly constant, 218 ± 29 (mean ± s.d.) days old, disregarding the localities, but a weak positive correlation was obtained between age and sampling date or timing of recruitment. Body length at recruitment was also constant, 56.3 ± 2.3 mm s.l., and showed no significant correlation with either locality or recruitment timing. Estimated birth date ranged from April to November, the mean ± s.d. being 22 July 1982 ± 42 days, suggesting a peak season spawning in summer. Birth date was closely related with both the latitude of location and sampling time. Pigmentation developed more at lower latitude. Recruitment mechanism of the Japanese eel was summarized as follows: the earlier-born individual recruits earlier at lower latitude and at younger age, but at a constant body size. 相似文献
5.
ABSTRACT. Wild caught horseflies, Tabanus nigrovittatus Macq. (Diptera, Tabanidae), were presented solutions of 0.15 MNaCl at 37°C containing various concentrations of ATP, ADP, AMP, adenosine, phytic acid or 2,3-diphosphoglycerate in an artificial feeding apparatus. The insects fed upward through a Para-film M® membrane. ADP (ED50 35 μM) was more potent than ATP (ED50 112 μM) and AMP (ED50 382 mUM). All of these diets were deposited in the midgut, an indication that the flies were in the 'blood feeding' mode. Adenosine caused only 23% gorging at 1 mM. Phytic acid caused only 10% gorging at 1 mM and 2,3-diphosphoglyceric acid had no activity at 0.6 mM. Flies would feed only in highly reflective cages under high levels of light intensity (1200–1500 lux) at the membrane surface. 相似文献
6.
Motility and orientation has been studied in the unicellular photosynthetic flagellate, Euglena gracilis, using real time image analysis capable of tracking up to 200 cells simultaneously in the slow rotating centrifuge microscope (NIZEMI) which allows one to observe the cells' swimming behavior during centrifugation accelerations between 1 g and 5 g. At 1 g the cells show a weak negative gravitaxis, which increases significantly at higher accelerations up to about 3 g. Though most cells were capable of swimming even against an acceleration of 4.5 g, the degree of gravitaxis decreased and some of the cells were passively moved downward by the acceleration force; this is true for most cells at 5 g. The velocity of cells swimming against 1 g is about 10% lower than that of cells swimming in other directions. The velocity decreases even more drastically in cells swimming against higher acceleration forces than those at 1 g. The degree of gravitactic orientation drastically decreases after short exposure to artificial UV radiation which indicates that gravitaxis may be due to an active physiological perception rather than a physical effect such as an asymmetry of the center of gravity within the cell.
Offprint requests to: D.-P. Häder 相似文献
7.
Sina M. Adl David Bass Christopher E. Lane Julius Luke Conrad L. Schoch Alexey Smirnov Sabine Agatha Cedric Berney Matthew W. Brown Fabien Burki Paco Crdenas Ivan epi
ka Lyudmila Chistyakova Javier del Campo Micah Dunthorn Bente Edvardsen Yana Eglit Laure Guillou Vladimír Hampl Aaron A. Heiss Mona Hoppenrath Timothy Y. James Anna Karnkowska Sergey Karpov Eunsoo Kim Martin Kolisko Alexander Kudryavtsev Daniel J.G. Lahr Enrique Lara Line Le Gall Denis H. Lynn David G. Mann Ramon Massana Edward A.D. Mitchell Christine Morrow Jong Soo Park Jan W. Pawlowski Martha J. Powell Daniel J. Richter Sonja Rueckert Lora Shadwick Satoshi Shimano Frederick W. Spiegel Guifr Torruella Noha Youssef Vasily Zlatogursky Qianqian Zhang 《The Journal of eukaryotic microbiology》2019,66(1):4-119
8.
9.
10.
11.
12.
Nizin is produced by Str. lactis, strain MSU. During biosynthesis it is excreted into the fermentation broth and gradually adsorbed on the organism cells. This was confirmed by experiments with an inactive variant of Str. lactis IIa. The cells of this culture adsorbed nizin from "active" fermentation broth. Adsorption of nizin depended on pH of the medium; at pH 2,3 the cells did not adsorbe the antibiotic and at pH 6.6 the amount of the antibiotic adsorbed by the cells was maximum. 相似文献
13.
14.
Immature gerbils (4 weeks of age or less) proved to be more resistant to Babesia divergens infections than did mature animals (greater than 8 weeks old). Nutritional studies showed that a milk diet contributes little to this innate resistance. Similarly, the apparent predilection of B. divergens for mature erythrocytes, and also in vitro serum incubation studies, suggested that blood factors do not contribute to any major degree. Splenectomy of immature gerbils abolished their nonspecific resistance and studies with splenocyte incubations with the piroplasm imply that splenic integrity is necessary for the maintenance of this, apparently antibody-independent, resistance to infection with B. divergens. 相似文献
15.
Kenneth E. Lipson Guodong Liang Li Xia Xiaoxia Gai Michael B. Prystowsky Xianzhi Mao 《Journal of cellular biochemistry》1995,57(4):711-723
Mobility shift assays were used to examine protein binding to the human TK gene CCAAT boxes. Similar protein binding patterns were observed with probes containing either the proximal or distal CCAAT. However, probes containing both CCAAT boxes in which one of the CCAAT boxes was inactivated by mutation did not demonstrate identical binding patterns. One of the complexes formed with the longer probes was only observed when the distal CCAAT was intact. This species was not formed with probes that only contained an intact proximal CCAAT, and its formation could only be competed by oligonucleotides containing the distal CCAAT motif. This observation reveals the existence of a protein that can bind to the distal, but not to the proximal, CCAAT of the human TK promoter. This protein may account for the previous observation that the two CCAAT motifs are not functionally equivalent. The protein that binds to the distal, but not to the proximal, CCAAT (DTK-CBP) was also present in two human cell lines. Significantly more DTK-CBP was present in nuclear extracts of HepG2 and WI38 cells than in TK?ts13 cells. However, this protein was not observed in three different murine cell lines and one primary culture. Its abundance in some human cell lines suggests it might modulate the expression of human TK mRNA in cells that express this protein. 相似文献
16.
During host-specificity tests of the rust Uromyces heliotropii carried out in Europe against a range of cultivated and wild plants, two species of European heliotropes closely related to the summer annual weed, common heliotrope (Heliotropium europaeum) and one native to Australia, H. crispatum, were found to be susceptible. Detailed observations were made of the degree of infection, the climatic requirements, the attenuation of virulence over time and the low probability of spore transmission for infection. These results, in addition to the scattered, relatively uncommon occurrence of H. crispatum in the field, showed that the plant was unlikely to be adversely affected by the rust, which has been released in Australia to control common heliotrope infestations. 相似文献
17.
Abstract Movements of tui (Prosthetnadera novaeseelandiae) breeding on Tiritiri Matangi Island were followed and related to changes in nectar access. The island has limited nectar sources and birds must move to gain access to nectar year round. All tui leave the island, although the extent and timing of movement varies for different individuals. Tui return for breeding but again the timing of the return varies for individuals. Nectar sources were typically concentrated in both time and space, and access was related to individual asymmetries such as sex, residential status and age. These same asymmetries also relate to the timing of individuals movements, and where individuals nest relative to nectar sources. At concentrated food sources, access was determined by an absolute social hierarchy. Although tui defend exclusive feeding territories, breeding territories are not exclusive and access to nectar is determined by distance to nest site. Such variability in spatial organisation is related to a continuum concept of social organisation. 相似文献
18.
19.