共查询到20条相似文献,搜索用时 15 毫秒
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Anna N. Kondakova Olga V. Bystrova Sergey A. Ivanov Alexander S. Shashkov Andrey P. Anisimov 《Carbohydrate research》2009,344(4):531-534
The O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Yersinia pseudotuberculosis O:4a and studied by NMR spectroscopy, including 2D ROESY and 1H, 13C HMBC experiments. The following structure of the pentasaccharide repeating unit of the polysaccharide was established, which differs from the structure reported earlier [Gorshkova, R. P. et al., Bioorg. Khim. 1983, 9, 1401-1407] in the linkage modes between the monosaccharides: where Tyv stands for 3,6-dideoxy-d-arabino-hexose (tyvelose). The structure of the Y. pseudotuberculosis O:4a antigen resembles that of Y. pseudotuberculosis O:2c, which differs in the presence of abequose (3,6-dideoxy-d-xylo-hexose) in place of tyvelose only. 相似文献
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Nikolay P. Arbatsky Alexander S. Shashkov Lu Feng Yuriy A. Knirel Lei Wang 《Carbohydrate research》2010,345(14):2090-2594
Studies by sugar analysis and partial acid hydrolysis along with one- and two-dimensional 1H and 13C NMR spectroscopy and high-resolution ESI MS showed that the O-polysaccharide (O-antigen) Cronobacter sakazakii ATCC 29004 (serotype O2) possesses a branched hexasaccharide O-unit with a randomly mono-O-acetylated terminal rhamnose residue in the side chain and the following structure:A similar structure has been reported for the O-polysaccharide of C. sakazakii 767, which differs in the presence of an additional lateral α-d-Glcp residue on GlcNAc and the pattern of O-acetylation (Czerwicka, M., Forsythe, S. J.; Bychowska, A.; Dziadziuszko, H.; Kunikowska, D.; Stepnowski, P.; Kaczynski, Z. Carbohydr. Res.2010, 345, 908-913). 相似文献
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Kondakova AN Bystrova OV Shaikhutdinova RZ Ivanov SA Dentovskaya SV Shashkov AS Knirel YA Anisimov AP 《Carbohydrate research》2008,343(14):2486-2488
Structures of the O-antigens of Yersinia pseudotuberculosis O2c and O3 were reinvestigated by NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, (1)H,(13)C HSQC, and HMBC experiments. The following revised structure of the O2c tetrasaccharide repeating unit was established, which differs from the structure proposed earlier in the glycosylation pattern of the mannose residue at the branching point: where Abe stands for 3,6-dideoxy-d-xylo-hexose. The structure of the Y. pseudotuberculosis O3 antigen reported earlier was confirmed. 相似文献
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Angelina A. Parkhomchuk Nina A. Kocharova Alexander S. Shashkov Yuriy A. Knirel 《Carbohydrate research》2010,345(9):1235-1239
The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O12. Its structure was studied by sugar analysis using GLC of the alditol acetates and (S)-2-octyl glycosides, methylation analysis, Smith degradation, and 1H and 13C NMR spectroscopy, including 2D 1H-1H COSY, TOCSY, ROESY, 1H-13C HSQC, and HMBC experiments. It was found that the polymer is a neutral heteropolysaccharide and has a branched heptasaccharide repeating unit with the following structure: 相似文献
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Perepelov AV Liu B Senchenkova SN Shashkov AS Feng L Knirel YA Wang L 《Carbohydrate research》2007,342(17):2676-2681
The O-polysaccharide was isolated from the lipopolysaccharide of Escherichia coli O168 and studied by chemical analyses and Smith degradation along with (1)H and (13)C NMR spectroscopies. The following structure of the branched pentasaccharide repeating unit of the O-polysaccharide was established: [carbohydrate structure: see text] where 6-O-acetylation of GlcNAc is partial. Reinvestigation of the O-polysaccharide of Shigella dysenteriae type 4 established earlier showed it to have the same structure except for that the lateral Fuc residue is nonstoichiometrically O-acetylated at each position. 相似文献
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Enteric bacteria have developed various survival systems that protect against acid stress. In this study, an aspartate-dependent acid survival system is characterized in Yersinia pseudotuberculosis. The expression of aspartase (AspA) was confirmed to be increased at acidic pH by proteomic and lacZ fusion analyses. Addition of aspartate increased acid survival of the wild type but not the aspA knockout mutant. AspA increases acid survival by producing ammonia as demonstrated by mutation and in vitro enzyme activity analyses. This is the first demonstration that an enzyme involved in aspartate metabolism plays a role in acid survival in an enteric bacterium. 相似文献
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Ovchinnikova OG Kocharova NA Parkhomchuk AA Bialczak-Kokot M Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2011,346(2):377-380
An O-polysaccharide (O-antigen) was isolated by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O60 and studied by sugar and methylation analyses as well as 1H and 13C NMR spectroscopy, including 2D ROESY and 1H,13C HMBC experiments in D2O and a ROESY experiment in a 9:1 H2O–D2O mixture to reveal correlations for NH protons. It was found that the polysaccharide is built up of linear pentasaccharide repeating units containing an amide of d-glucuronic acid with l-serine and has the following structure:The O-antigen studied is structurally and serologically closely related to the O-antigen of Proteus vulgaris O44. 相似文献
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Ovchinnikova OG Bushmarinov IS Kocharova NA Toukach FV Wykrota M Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2007,342(8):1116-1121
The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide from Providencia alcalifaciens O27 and studied by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, H-detected (1)H,(13)C HSQC, and HMBC experiments. It was found that the polysaccharide is built up of linear partially O-acetylated tetrasaccharide repeating units and has the following structure: [structure: see text] where Qui4NFo stands for 4-formamido-4,6-dideoxyglucose (4-formamido-4-deoxyquinovose). The O-polysaccharide structure of Providencia stuartii O43 established earlier was revised with respect to the configuration of the constituent 4-amino-4,6-dideoxyhexose (from Rha4N to Qui4N). 相似文献
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The O-polysaccharide was isolated by mild acid hydrolysis of the lipopolysaccharide of Rahnella aquatilis 1-95 and studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including NOESY and 1H,13C HSQC experiments for linkage and sequence analysis. The following structure of the branched trisaccharide repeating unit of the O-polysaccharide was established: [carbohydrate structure: see text]. 相似文献
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Ovchinnikova OG Kocharova NA Bakinovskiy LV Torzewska A Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2004,339(15):2621-2626
The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia stuartii O47:H4, strain 3646/51. Studies by sugar and methylation analyses along with Smith degradation and 1H and 13C NMR spectroscopy, including two-dimensional 1H,1H COSY, TOCSY, ROESY and H-detected 1H,13C HSQC and HMBC experiments, showed that the polysaccharide has a branched hexasaccharide repeating unit with the following structure: [carbohydrate structure: see text] 相似文献
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The O-polysaccharide from Vibrio cholerae O6 was isolated from the LPS by mild-acid hydrolysis and has been investigated by sugar and methylation analysis and NMR spectroscopy. The polysaccharide was also depolymerized with aqueous hydrofluoric acid to give the repeating unit and multiples thereof. The O-polysaccharide had the following tetrasaccharide repeating unit. Two O-acetyl groups are present, one of them making the GlcNAc residue fully substituted and the steric crowding considerable at the branching residue. 相似文献
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Katzenellenbogen E Kocharova NA Zatonsky GV Korzeniowska-Kowal A Shashkov AS Knirel YA 《Carbohydrate research》2003,338(20):2153-2158
An acidic O-polysaccharide isolated by mild acid hydrolysis from the lipopolysaccharide of Hafnia alvei PCM 1546 is composed of D-Gal, D-Glc, D-GlcA, D-GalNAc and O-acetyl groups in the ratios 1:1:1:2:1.6. On the basis of sugar and methylation analyses along with 1D and 2D 1H and 13C NMR spectroscopy, the following structure of the pentasaccharide repeating unit of the polysaccharide was established: [see equation in text]. 相似文献
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Kocharova NA Maszewska A Zatonsky GV Bystrova OV Ziolkowski A Torzewska A Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2003,338(13):1425-1430
The O-polysaccharide (O-antigen) of Providencia alcalifaciens O21 was obtained by mild acid degradation of the lipopolysaccharide and studied by chemical methods and NMR spectroscopy. It was found that the polysaccharide is built up of branched pentasaccharide repeating units with a terminal residue of 3-formamido-3,6-dideoxy-D-galactose (D-Fuc3NFo) and has the following structure: [structure: see text]. Anti-P. alcalifaciens O21 serum cross-reacted with the O-antigen of Proteus vulgaris O47, which contains a GalNAc trisaccharide similar to that present in the P. alcalifaciens O21 O-polysaccharide. 相似文献
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A similarity in the O-acetylation pattern of the O-antigens of Shigellaflexneri types 1a, 1b, and 2a
Andrei V. Perepelov Vyacheslav L. L’vov Sof’ya N. Senchenkova Alexander S. Shashkov Petr G. Aparin Yuriy A. Knirel 《Carbohydrate research》2009,344(5):687-692
Shigella flexneri type 2a is the first, and type 1b is the second, most prevalent isolates from patients with shigellosis in Russia. The O-specific polysaccharides (OPSs, O-antigens) of S. flexneri types 1-5 possess a common →2)-α-l-RhapIII-(1→2)-α-l-RhapII-(1→3)-α-l-RhapI-(1→3)-β-d-GlcpNAc-(1→ backbone and differ from each other in its glucosylation or/and O-acetylation at various positions, the modifications being responsible for various O-factors. It was suggested that O-factor 6 expressed by type 1b is associated with O-acetylation of RhaI at position 2 but more than one O-acetyl group has been detected in the type 1b OPS [Kenne, L. et al. Eur. J. Biochem.1978, 91, 279-284]. In this work, O-acetylation of RhapI in the type 1b OPS was confirmed by NMR spectroscopy and location of an additional O-acetyl group at position either 3 (major) or 4 (minor) of RhapIII was determined. Type 1a differs from type 1b in the lack of O-acetylation of RhapI only. In type 2a, in addition to two reported major O-acetyl groups at position 6 of GlcNAc and position 3 of RhapIII [Kubler-Kielb, J. et al. Carbohydr. Res.2007, 342, 643-647], a minor O-acetyl group was found at position 4 of RhaIII. Therefore, RhapIII is O-acetylated in the same manner in all three S. flexneri serotypes studied. 相似文献
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Perepelov AV Weintraub A Liu B Senchenkova SN Shashkov AS Feng L Wang L Widmalm G Knirel YA 《Carbohydrate research》2008,343(5):977-981
The O-antigen structure of Shigella dysenteriae type 2 was reinvestigated using chemical modifications along with high-resolution 2D (1)H and (13)C NMR spectroscopy. The O-antigen was found to contain a pyruvic acid acetal, which was overlooked in an early study, and the following revised structure of the pentasaccharide repeating unit was established: where approximately 70% GlcNAc residues bear an O-acetyl group at position 3. The O-antigen of Escherichia coli O112ac was found to have the same carbohydrate structure but to lack O-acetylation. 相似文献