共查询到20条相似文献,搜索用时 15 毫秒
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We have recently described Arabidopsis cell suspension cultures that can be effectively synchronised. Here, we describe procedures that allow clonal-transformed cell suspension lines to be produced using Agrobacterium-mediated transformation, and an optimised and straightforward procedure for the cryopreservation and recovery of both parental and transformed lines. Frozen cultures show 90% viability and rapid re-growth after recovery. We show that the cryopreservation procedure is equally applicable to the frequently used tobacco bright yellow (BY)2 cell suspension culture, and that cell cycle synchronisation capacity of parental lines is maintained after both transformation and recovery from cryopreservation. The techniques require no specialised equipment, and are suitable for routine laboratory use, greatly facilitating the handling and maintenance of cell cultures and providing security against both contamination and cumulative somaclonal variation. Finally, the ability to store easily large numbers of transformed lines opens the possibility of using Arabidopsis cell suspension cultures for high-throughput analysis. 相似文献
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We have been able to demonstrate that a fraction of DNA becomes crosslinked to nuclear lamina shells isolated from Ehrlich ascites tumour cells irradiated with UV light. Terminal labeling of short DNA fragments covalently attached to proteins reveals that DNA has become crosslinked to all three lamins and to a protein comigrating with vimentin. 相似文献
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ABO3, a WRKY transcription factor,mediates plant responses to abscisic acid and drought tolerance in Arabidopsis 总被引:1,自引:0,他引:1
Xiaozhi Ren Zhizhong Chen Yue Liu Hairong Zhang Min Zhang Qian Liu Xuhui Hong Jian‐Kang Zhu Zhizhong Gong 《The Plant journal : for cell and molecular biology》2010,63(3):417-429
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AtMyb7, a subgroup 4 R2R3 Myb,negatively regulates ABA‐induced inhibition of seed germination by blocking the expression of the bZIP transcription factor ABI5 下载免费PDF全文
JUN HYEOK KIM WOO YOUNG HYUN HOAI NGUYEN NGUYEN CHAN YOUNG JEONG LIMING XIONG SUK‐WHAN HONG HOJOUNG LEE 《Plant, cell & environment》2015,38(3):559-571
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The DOF transcription factor OBP1 is involved in cell cycle regulation in Arabidopsis thaliana 总被引:2,自引:0,他引:2
Skirycz A Radziejwoski A Busch W Hannah MA Czeszejko J Kwaśniewski M Zanor MI Lohmann JU De Veylder L Witt I Mueller-Roeber B 《The Plant journal : for cell and molecular biology》2008,56(5):779-792
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Zhenzhou Cheng Nan Zhang Le Chang Pengfei Qi Lin Zhang Lie Lin Yan Wang Weiwei Liu 《Journal of biophotonics》2023,16(2):e202200160
Myopia and keratoconus have become common corneal diseases that threaten the quality of human vision, and keratoconus is one of the most common indications for corneal transplantation worldwide. Collagen crosslinking (CXL) using riboflavin and ultraviolet A (UVA) light is an effective approach for treating ophthalmic disorders and has been shown clinically not only to arrest further progression of keratoconus but also to improve refractive power for cornea. However, CXL surgery irradiated by UVA has various potential risks such as surface damage and endothelial cell damage. Here, near-infrared femtosecond laser-based two-photon CXL was first applied to ex vivo human corneal stroma, operating at low photon energy with high precision and stability. After two-photon CXL, the corneal stiffness can be enhanced by 300% without significantly reducing corneal transparency. These findings illustrate the optimized direction that depositing high pulses energy in corneal focal volume (not exceeding damage threshold), and pave the way to 3D CXL of in vivo human cornea with higher safety, precision, and efficacy. 相似文献