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Immortalization of multipotent growth-factor dependent hemopoietic progenitors from mice transgenic for GATA-1 driven SV40 tsA58 gene. 总被引:2,自引:0,他引:2
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L A Cairns S Crotta M Minuzzo E Moroni F Granucci S Nicolis R Schiró L Pozzi B Giglioni P Ricciardi-Castagnoli et al. 《The EMBO journal》1994,13(19):4577-4586
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Erythropoietin stimulates proliferation and interferes with differentiation of myoblasts 总被引:15,自引:0,他引:15
Ogilvie M Yu X Nicolas-Metral V Pulido SM Liu C Ruegg UT Noguchi CT 《The Journal of biological chemistry》2000,275(50):39754-39761
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Phosphatidylinositol 3-kinase/Akt induced by erythropoietin renders the erythroid differentiation factor GATA-1 competent for TIMP-1 gene transactivation
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Kadri Z Maouche-Chretien L Rooke HM Orkin SH Romeo PH Mayeux P Leboulch P Chretien S 《Molecular and cellular biology》2005,25(17):7412-7422
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A multipotent immature myeloid cell population was produced from a basic fibroblast growth factor (bFGF)-dependent hematopoietic stem cell line, A-6, when cultured with stem cell factor (SCF) replacing bFGF. Those cells were positive for stem cell markers, c-kit and CD34, and a myeloid cell marker, F4/80. Some cell fractions were also positive for Mac-1, a macrophage marker or Gr-1, a granulocytic maker, but negative for an erythroid marker TER119. They also showed the expression of mRNA for the myeloid-specific PU.1 but did not that for the erythroid-specific GATA-1. Among various cytokines, interleukin-3 (IL-3) induced erythroid precursor cells that expressed the erythroid-specific GATA-1 and beta-major globin. The quantitative analysis showed that erythroid precursor cells were newly produced from the immature myeloid cells by cultivation with IL-3. SCF and IL-3 induced stepwise generation of erythroid precursor cells from an A-6 hematopoietic stem cell line. 相似文献
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