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1.
A tomato ( Lycopersicon esculentum Mill. cv. Pera) callus culture tolerant to NaCl was obtained by successive subcultures of NaCl-sensitive calli in medium supplemented with 50 m M NaCl. NaCl-tolerant calli grew better than NaCl-sensitive calli in media supplemented with 50 and 100 m M NaCl. Analysis of callus ion content showed a strong increase in Na+ and Cl− both in NaCl-tolerant and -sensitive calli grown in media containing NaCl for one subculture. Cells from NaCl-tolerant calli showed a higher H+ extrusion activity than those from NaCl-sensitive calli grown for one subculture in the presence of NaCl. The inhibition of H+ extrusion by NaCl-sensitive cells was correlated with an inhibition of microsomal vanadate-sensitive H+ -ATPase (EC 3.6.1.35) and ATP-dependent H+ transport, while the stimulation of H+ extrusion by cells tolerant to 50 m M NaCl was correlated with an increase in plasma membrane ATP-dependent H+ transport. The increase of ATP-dependent H+ extrusion in plasma membranes isolated from 50 m M NaCl-tolerant calli was not a result of stimulation of a vanadate-sensitive ATP hydrolytic activity or an increase in passive permeability to H+ . Relative to NaCl-sensitive calli, plasma membrane H+ -ATPase from calli tolerant to 50 m M NaCl showed a lower Km for Mg2+ -ATP. Our results indicate that tolerance of tomato calli to 50 m M NaCl increases the affinity of plasma membrane H+ -ATPase for the substrate ATP and stimulates the H+ -pumping activity of this enzyme without modifying its phosphohydrolytic activity. 相似文献
2.
Two tomato ( Lycopersicon esculentum Mill. cv. Pera) callus lines tolerant to NaCl were obtained by successive subcultures of NaCl-sensitive calli in 50 and 100 m M NaCl-supplemented medium. Growth and ion content, as well as plasma membrane lipid composition, fluidity and H+ -ATPase (EC 3.6.1.35) activity, were studied in both NaCl-sensitive and NaCl-tolerant calli. Although calli tolerant to 100 m M NaCl exhibited a reduced growth relative to calli sensitive to NaCl or tolerant to 50 m M NaCl, growth of calli tolerant to 100 m M NaCl was higher than that of NaCl-sensitive calli grown for one subculture in 100 m M NaCl. Growth in the presence of 100 m M NaCl provoked an increase of Na+ and Cl− content, but no significant changes in K+ and Ca2+ . As compared with NaCl-sensitive and 50 m M NaCl-tolerant calli, plasma membrane vesicles isolated from calli tolerant to 100 m M NaCl exhibited a higher phospholipid and sterol content as well as a lower phospholipid/free sterol ratio and a lower double bond index (DBI) of phospholipid fatty acids. The changes in plasma membrane lipid composition were correlated with a decrease of plasma membrane fluidity in calli tolerant to 100 m M NaCl, as indicated by fluorimetric studies using diphenylhexatriene (DPH) as probe. Plasma membrane-enriched vesicles isolated from calli tolerant to 100 m M NaCl showed lower ATP hydrolysis and ATP-dependent H+ -pumping activities, as well as a lower passive permeability to H+ than plasma membrane from NaCl-sensitive and 50 m M NaCl-tolerant calli. The involvement of the changes in plasma membrane lipid content and composition, fluidity and H+ -ATPase activity in salt tolerance of tomato calli is discussed. 相似文献
3.
The regulation of the H+ -ATPase of plasma membrane is a crucial point in the integration of transport processes at this membrane. In this work the regulation of H+ -ATPase activity induced by changes in turgor pressure was investigated and compared with the stimulating effect of fusicoccin (FC). The exposure of cultured cells of Arabidopsis thaliana L. (ecotype Landsberg 310–14-2) to media containing mannitol (0. 15 or 0. 3 M ) or polyethylene glycol 6000 (PEG) (15. 6% or 22% w/v) resulted in a decrease in the turgor pressure of the cells and in a strong stimulation of H+ extrusion in the incubation medium. The osmotica-induced H+ extrusion was (1) inhibited by the inhibitor of plasma membrane H+ -ATPase, erythrosin B (EB), (2) dependent on the external K+ concentration, (3) associated with a net K+ influx, and (4) lead to an increase of cellular malate content. These results show that the reduction of external osmotic potential stimulates the activity of plasma membrane H+ -ATPase
The effect of mannitol was only partially inhibited by treatments with cycloheximide (CH) and cordycepin, which block protein and mRNA synthesis, respectively. All the effects of osmotica were qualitatively and quantitatively similar to those induced by 5 μ M FC. However, when FC and mannitol (or PEG) were fed together, their effects on H+ extrusion appeared synergistic, irrespective of whether FC was present at suboptimal or optimal concentrations. This behaviour suggests that the modes of action of FC and of the osmotica on H+ -ATPase activity differ at least in some step(s) 相似文献
The effect of mannitol was only partially inhibited by treatments with cycloheximide (CH) and cordycepin, which block protein and mRNA synthesis, respectively. All the effects of osmotica were qualitatively and quantitatively similar to those induced by 5 μ M FC. However, when FC and mannitol (or PEG) were fed together, their effects on H
4.
As water and nutrient uptake should be related in the response of plants to salinity, the aim of this paper is to establish whether or not aquaporin functionality is related to H+ -ATPase activity in root cells of pepper ( Capsicum annuum L.) plants. Thus, H+ -ATPase activity was measured in plasma membrane vesicles isolated from roots and aquaporin functionality was measured using a cell pressure probe in intact roots. Salinity was applied as 60 m M NaCl or 60 m M KCl, to determine which ion (Na+ , K+ or Cl− ) is producing the effects. We also investigated whether the effects of both salts were ameliorated by Ca2+ . Similar results were obtained for cell hydraulic conductivity, Lpc , and H+ -ATPase activity, large reductions in the presence at NaCl or KCl and an ameliorative effect of Ca2+ . However, fusicoccin (an activator of H+ -ATPase) did not alter osmotic water permeability of protoplasts isolated from roots. Addition of Hg2+ inhibited both ATPase and aquaporins, but ATPase also contains Hg-binding sites. Therefore, the results indicate that H+ -ATPase and aquaporin activities may not be related in pepper plants. 相似文献
5.
In vivo and in vitro effects of boron on the plasma membrane proton pump of sunflower roots 总被引:3,自引:0,他引:3
Marta Roldán rés Belver Pilar Rodríguez-Rosales Nuria Ferrol Juan Pedro Donaire 《Physiologia plantarum》1992,84(1):49-54
The effect of boron excess and deficiency on H+ efflux from excised roots from sunflower ( Heliarahus annuus L. cv. Enano) seedlings and on plasma membrane H+ -ATPase (EC 3.6.1.35) in isolated KI-washed microsomes has been investigated. When seedlings were grown in media with toxic levels of H3 BO3 (5 m M ) or without added boron and exposed to light conditions, an inhibition of the capacity for external acidification by excised roots was observed as compared to roots from seedlings grown with optimal H3 BO3 concentration (0.25 m M ). Toxic and deficient boron conditions also inhibited the vanadate-sensitive H+ -ATPase of microsomes isolated from the roots. The mechanism of boron toxicity was investigated in vitro with microsorne vesicles. A strong effect of boron on the vanadate-sensitive, ATP-dependent H+ transport was found, but the vanadate-sensitive phospho-bydrolase activity was not affected. These results suggest that boron could exert an effect on the plasma membrane properties, directly or indirectly regulating, proton transport. 相似文献
6.
Lone Baunsgaard Kees Venema Kristian B. Axelsen José Manuel Villalba Annikki Welling Bernd Wollenweber Michael G. Palmgren 《The Plant journal : for cell and molecular biology》1996,10(3):451-458
Transport across the plasma membrane is driven by an electrochemical gradient of H+ ions generated by the plasma membrane proton pump (H+ -ATPase). Random mutants of Arabidopsis H+ -ATPase AHA1 were isolated by phenotypic selection of growth of transformed yeast cells in the absence of endogenous yeast H+ -ATPase (PMA1). A Trp-874-Leu substitution as well as a Trp-874 to Lys-935 deletion in the hydrophilic C-terminal domain of AHA1 conferred growth of yeast cells devoid of PMA1. A Trp-874-Phe substitution in AHA1 was produced by site-directed mutagenesis. The modified enzymes hydrolyzed ATP at 200–500% of wild-type level, had a sixfold increase in affinity for ATP (from 1.2 to 0.2 mM; pH 7.0), and had the acidic pH optimum shifted towards neutral pH. AHA1 did not contribute significantly to H+ extrusion by transformed yeast cells. The different species of aha1, however, displayed marked differences in initial rates of net H+ extrusion and in their ability to sustain an electrochemical H+ gradient. These results provide evidence that Trp-874 plays an important role in auto-inhibition of the plant H+ -ATPase and may be involved in controlling the degree of coupling between ATP hydrolysis and H+ pumping. Finally, these results demonstrate the usefulness of yeast as a generalized screening tool for isolating regulatory mutants of plants transporters. 相似文献
7.
Lobão FA Façanha AR Okorokov LA Dutra KR Okorokova-Façanha AL 《FEMS microbiology letters》2007,274(1):17-23
The effect of aluminum on dimorphic fungi Yarrowia lipolytica was investigated. High aluminum (0.5–1.0 mM AlK(SO4 )2 ) inhibits yeast–hypha transition. Both vanadate-sensitive H+ transport and ATPase activities were increased in total membranes isolated from aluminum-treated cells, indicating that a plasma membrane H+ pump was stimulated by aluminum. Furthermore, Al-treated cells showed a stronger H+ efflux in solid medium. The present results suggest that alterations in the plasma membrane H+ transport might underline a pH signaling required for yeast/hyphal development. The data point to the cell surface pH as a determinant of morphogenesis of Y. lipolytica and the plasma membrane H+ -ATPase as a key factor of this process. 相似文献
8.
Anja Thoe Fuglsang Grete Tulinius Na Cui Michael Gjedde Palmgren 《Physiologia plantarum》2006,128(2):334-340
The activity of the plant plasma membrane H+ -ATPase is tightly regulated via phosphorylation and binding of 14-3-3 protein to the C-terminus of the pump. Whereas the 14-3-3-binding mechanism has been described in detail, the identity of specific protein kinases and phosphatases involved in the control of 14-3-3 binding has remained elusive. Using the yeast two-hybrid system, GST pull-down assays and overlay experiments, we report that scaffolding subunit A of protein phosphatase 2A (PP2A-A) interacts with the C-terminus of the Arabidopsis plasma membrane H+ -ATPase isoform 2. PP2A-A binding is inhibited in the presence of 14-3-3 protein and fusicoccin, a fungal toxin which induces binding of 14-3-3 protein to the C-terminal end of the plasma membrane H+ -ATPase. This indicates that PP2A-A and 14-3-3 protein compete with each other for binding to the same region in the C-terminus of the H+ -ATPase. 相似文献
9.
10.
Marla L. Binzel 《Physiologia plantarum》1995,94(4):722-728
NaCl-induced changes in the accumulation of message for the 70 kDa subunit of the tonoplast H+ -ATPase and plasma membrane H+ -ATPase were studied in hydroponically grown plants of Lycopersicon esculentum Mill. cv. Large Cherry Red. There was increased accumulation of message for the 70 kDa (catalytic) subunit of the tonoplast H+ -ATPase in expanded leaves of tomato plants 24 h after final NaCl concentrations were attained. This was a tissue-specific response; levels of this message were not elevated in roots or in young, unexpanded leaves. The NaCl-induced accumulation of this message was transient in the expanded leaves and returned to control levels within 7 days. The temporal and spatial patterns of NaCl-induced accumulation of message for the plasma membrane H+ -ATPase differed from the patterns associated with the 70 kDa subunit of the tonoplast H+ -ATPase. NaCl-induced accumulation of the plasma membrane H+ -ATPase message occurred in both roots and expanded leaves. Initially accumulation of the plasma membrane H+ -ATPase message was greater in root tissue than in expanded leaves, but increased to higher levels in expanded leaves after 7 days. These results suggest that increased expression of the tonoplast H+ -ATPase is an early response to salinity stress and may be associated with survival mechanisms, rather than with long-term adaptive processes. 相似文献
11.
Fang-Sik Che Kunihiro Kasamo Naoyuki Fukuchi Akira Isogai Akinori Suzuki 《Physiologia plantarum》1992,86(4):518-524
Syringostatin is a newly discovered phytotoxin produced by a phytopathogenic bacterium Pseudomonas syringae pv. syringae lilac isolate. The effects of syringostatin and the similar phytotoxins, syringomycin and syringotoxin, on H− -ATPase activity were investigated using cultured mung bean ( Vigna radiata L. cv. Ryokuto) cells or plasma membrane vesicles isolated from mung bean hypocotyls. 31 P-NMR analysis of cultured cells treated with syringostatin revealed that the cytoplasmic pH was decreased. When plasma membrane was prepared by a two-step method (Dextran gradient followed by a sucrose gradient). syringostatin, syringomycin and syringotoxin inhibited the H+ -ATPase activity in a dose-dependent manner. In contrast, these toxins stimulated H+ -ATPase activity when plasma membrane was prepared by a one-step method (sucrose gradient). While these toxins inhibited the H+ -ATPase activity of inside-out plasma membrane vesicles, the H+ -ATPase activity of right-side-out vesicles was stimulated. The detergent. Triton X-100, abolished this stimulatory effect of the toxins on the H+ -ATPase of right-side-out vesicles and of one-step purified plasma membrane. The toxins also inhibited the activity of the plasma membrane H+ -ATPase solubilized with deoxycholate and Zwittergent 3–14. Taken together, these results indicate that these toxins exert their effects partly by a detergent-like action on the plasma membrane and partly by inhibition of the enzyme. 相似文献
12.
The involvement of a vanadate-sensitive ATPase in plasma membranes of a salt tolerant cyanobacterium
Rachel Gabbay-Azaria Uri Pick Gozal Ben-Hayyim Elisha Tel-Or 《Physiologia plantarum》1994,90(4):692-698
Plasma membranes of the marine cyanobacterium Spirulina subsalsa were tested for ATPase activity, and for involvement in salt stress. Transition of cells from saline to hypersaline medium enhances the respiratory activity associated with extrusion of Na+ and Cl− , and persisting salt stress induces synthesis of respiratory enzymes in the plasma membranes. The membranes possess an ATPase, specific for ATP and Mg2+ and sensitive to orthovanadate and dicyclohexylcarbodiimide. Immunoblot analysis of plasma membrane polypeptides from Spirulina subsalsa with anti- Arabidopsis H+ -ATPase serum identified a single polypeptide of 100 kDa, which cross-reacted with the antibodies. An unusual feature of this ATPase is a specific stimulation by Na+ ions. Prolonged adaptation of S. subsals cells to hypersaline conditions induced an increase in ATPase activity in subsequent plasma membrane preparations, as well as a higher content of the 100 kDa polypeptide. It is suggested that the ATPase investigated is an H+ -pump, which is involved in extrusion of Na+ and in conferring resistance to salt stress. 相似文献
13.
M. Marra C. Olivari Sabina Visconti Cristina Albumi Patrizia Aducci Maria Ida De Michelis 《Plant biology (Stuttgart, Germany)》2000,2(1):11-16
Abstract: A putative consensus domain for binding of 14-3-3 proteins to the plasma membrane (PM) H+ -ATPase was identified in the highly-conserved sequence RSR(p)SWSF [where (p)S is Ser776 of the maize isoform MHA2], localized in the cytosolic stretch connecting transmembrane segments 8 and 9. A 15 amino acid biotinylated phosphopeptide comprising this motif: i) bound a recombinant 14-3-3 protein, ii) inhibited fusicoccin-induced stimulation of the PM H+ -ATPase activity both in PM isolated from germinating radish ( Raphanus sativus L.) seedlings and in ER isolated from Saccharomyces cerevisiae expressing AHA1 (an isoform of Arabidopsis thaliana PM H+ -ATPase), and iii) inhibited fusicoccin binding to PM isolated from germinating radish seedlings. The corresponding non-phosphorylated peptide was inactive in all the performed assays. Together, these results suggest that the cytosolic strand connecting transmembrane segments 8 and 9 of the PM H+ -ATPase is a 14-3-3 binding site which might cooperate with the C-terminal domain of the'enzyme in generating a stable association between the H+ -ATPase and 14-3-3 protein. 相似文献
14.
Topology and target interaction of the fusicoccin-binding 14-3-3 homologs of Commelina communis 总被引:3,自引:0,他引:3
Claudia Oecking Markus Piotrowski Jens Hagemeier Klaus Hagemann 《The Plant journal : for cell and molecular biology》1997,12(2):441-453
Upon binding to a high-affinity plasma membrane (PM) protein (a member of the 14-3-3 family of regulatory proteins), the fungal phytotoxin fusicoccin (FC) activates the H+ - ATPase by hindering the inhibitory interaction of the enzyme's C-terminus with its catalytic site. Protease protection experiments carried out with sealed PM vesicles of different orientation proved that the FC-binding site faces the cytoplasmic surface of the membrane. The in vivo induced activation of the H+ -ATPase by FC was retained during solubilization of PM proteins. Two-dimensional gel systems combining a native separation of membrane protein complexes with a denaturing dimension as well as high-performance anion-exchange chromatography proved the existence of a labile ATPase:14-3-3 complex in plasma membranes. Stabilization of this complex could be achieved by FC treatment in vivo or in vitro . Mild proteolytic removal of the C-terminal auto-inhibitory domain of the H+ ATPase liberated apparent hydrophobic 14-3-3 isoforms from the membrane in soluble form. During size exclusion chromatography of the proteolytically released proteins, co-elution of 14-3-3 dimers, protein-bound FC and the C-terminus of the H+ ATPase was observed. Moreover, the data suggest that 14-3-3 dimers themselves are not able to bind FC. Based on these results, it is proposed that the 'FC receptor' is represented by a labile complex between a 14-3-3 dimer and the H+ -ATPase whose formation is part of a mechanism regulating ATPase-activity under physiological conditions. In our working model, binding of FC stabilizes this labile complex, thus leading to a strong and persistent activation of the H+ -ATPase in vivo . The possibility that the C-terminus of the enzyme represents the binding domain for 14-3-3 homologs is discussed. 相似文献
15.
YIYONG ZHU TINGJUN DI GUOHUA XU XI CHEN HOUQING ZENG FENG YAN & QIRONG SHEN 《Plant, cell & environment》2009,32(10):1428-1440
The preference of paddy rice for NH4 + rather than NO3 - is associated with its tolerance to low pH since a rhizosphere acidification occurs during NH4 + absorption. However, the adaptation of rice root to low pH has not been fully elucidated. This study investigated the acclimation of plasma membrane H+ -ATPase of rice root to low pH. Rice seedlings were grown either with NH4 + or NO3 - . For both nitrogen forms, the pH value of nutrient solutions was gradually adjusted to pH 6.5 or 3.0. After 4 d cultivation, hydrolytic H+ -ATPase activity, V max , K m , H+ -pumping activity, H+ permeability and pH gradient across the plasma membrane were significantly higher in rice roots grown at pH 3.0 than at 6.5, irrespective of the nitrogen forms supplied. The higher activity of plasma membrane H+ -ATPase of adapted rice roots was attributed to the increase in expression of OSA1, OSA3, OSA7, OSA8 and OSA9 genes, which resulted in an increase of H+ -ATPase protein concentration. In conclusion, a high regulation of various plasma membrane H+ -ATPase genes is responsible for the adaptation of rice roots to low pH. This mechanism may be partly responsible for the preference of rice plants to NH4 + nutrition. 相似文献
16.
M. C. Bonza T. Fusca U. Homann G. Thiel & M. I. De Michelis 《Plant biology (Stuttgart, Germany)》2009,11(6):869-877
PPI1 (proton pump interactor isoform 1) is a novel protein able to interact with the C-terminal autoinhibitory domain of the Arabidopsis thaliana plasma membrane (PM) H+ -ATPase. In vitro, PPI1 binds the PM H+ -ATPase in a site different from the known 14-3-3 binding site and stimulates its activity. In this study, we analysed the intracellular localisation of PPI1. The intracellular distribution was monitored in A. thaliana cultured cells by immunolocalisation using an antiserum against the PPI1 N-terminus and in Vicia faba guard cells and epidermal cells by transient expression of a GFP::PPI1 fusion. The results indicate that the bulk of PPI1 is localised at the endoplasmic reticulum, from which it might be recruited to the PM for interaction with the H+ -ATPase in response to as yet unidentified signals. 相似文献
17.
18.
Marina S. Trofimova Irina N. Smolenskaya Artem V. Drabkin Alexander V. Galkin Alexey V. Babakov 《Physiologia plantarum》1997,99(2):221-226
Sugar beet ( Beta vulgaris L.) root suspension-cultured cells were converted to protoplasts which responded to fusicoccin (FC) by a rise in cytoplasmic pH (pHcyt ) averaging 0.25 units in the fluorimetric assay. This effect was blocked by erythrosin B, a specific inhibitor of the plasma membrane H+ -ATPase. A protein kinase inhibitor, staurosporine also caused cytosolic alkalinization that was sensitive to H+ -ATPase inhibitors. Most strikingly, the effect of staurosporine was suppressed by fusicoccin and vice versa. Addition of okadaic acid, entailing overall protein phosphorylation, also led to H+ -ATPase activation, whereupon fusicoccin lost its effect on proton transport. In parallel, kinetic and inhibitor analyses demonstrated that FC binding to the protoplast plasma membrane involved two sites with dissociation constants of 1 n M and 0.2 μ M and was indifferent to phosphorylation and dephosphorylation inhibitors. Thus, it could be concluded that (1) the effect of FC on cytoplasmic pH probably depends on the phosphorylation state of plasma membrane proteins and may have either sign; (2) the activation of H+ -ATPase by FC most likely proceeds directly through conformational receptor-enzyme interaction. 相似文献
19.
Inhibition of Glutamate Uptake and Proton Pumping in Synaptic Vesicles by S-Nitrosylation 总被引:1,自引:1,他引:0
Herman Wolosker Marcelo Reis Jamil Assreuy Leopoldo de Meis 《Journal of neurochemistry》1996,66(5):1943-1948
Abstract: Nitric oxide (NO; including NO• , NO+ , and NO− ) was found to inhibit glutamate uptake by isolated synaptic vesicles of rat brain. This was observed when two unrelated NO donors, S -nitrosogluthathione and S -nitroso- N -acetylpenicillamine, were used. The primary target of NO is the H+ -ATPase found in the synaptic vesicles, which leads to dissipation of the electrochemical proton gradient and inhibition of glutamate uptake. Oxyhemoglobin (12 µ M ) and, to a much lesser extent, methemoglobin protected the vacuolar H+ -ATPase from inhibition. Inhibition of H+ pumping by NO was reversed by addition of 0.5 m M dithiothreitol. The results indicate that the vacuolar H+ -ATPase from synaptic vesicles is inhibited by NO by a mechanism that involves S -nitrosylation of critical sulfhydryl groups in the enzyme. The interaction of NO with synaptic vesicles might be of importance for the understanding of the multiple effects of NO in neurotransmission. 相似文献
20.
In embryos of the sea urchin, Hemicentrotus pulcherrimus , as well as in cultured cells derived from isolated micromeres, spicule formation was inhibited by allylisothiocyanate, an inhibitor of H+ , K+ -ATPase, at above 0.5 μM and was almost completely blocked at above 10 μM. Amiloride, an inhibitor of Na+ , H+ antiporter, at above 100 μM exerted only slight inhibitory effect, if any, on spicule formation. Intravesicular acidification, determined using [ dimethylamine -14 C]-aminopyrine as a pH probe, was observed in the presence of ATP and 200 mM KCl in microsome fraction obtained from embryos at the post gastrula stage, at which embryos underwent spicule calcification. Intravesicular acidification and K+ -dependent ATPase activity were almost completely inhibited by allylisothiocyanate at 10 μM. Allylisothiocyanate-sensitive ATPase activity was found mainly in the mesenchyme cells with spicules isolated from prisms. H+ , K+ -ATPase, an H+ pump, probably mediates H+ release to accelerate CaCO3 deposition from Ca2+ , CO2 and H2 O in the primary mesenchyme cells. Intravesicular acidification was stimulated by valinomycin at the late gastrula and the prism stages but not at the pluteus stage. K+ permeability probably increases after the prism stage to activate H+ release. 相似文献