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1.
Germ line and somatic instability of a white mutation in Drosophila mauritiana due to a transposable genetic element 总被引:3,自引:0,他引:3
A spontaneous white mutation recovered in Drosophila mauritiana is unstable and reverts to normal eye color at a frequency greater than 4 per 1,000 X-chromosomes. Germ line reversion occurs at a high rate in D. mauritiana males and in interspecific hybrid females, while the rate is depressed in D. mauritiana females. These events are not restricted to the germ line, as cases of variegated patterns of eye pigmentation, indicating somatic reversion, are recovered at a frequency comparable to that of the male germ line reversion rate. Germ line reversion events are genetically stable, while the somatic variegation patterns are not heritable. The patterns of eye pigment variegation produced suggests that reversion events are occurring throughout development. Whole genome DNA digests blotted and probed with the cloned D. melanogaster white gene indicate that this unstable white mutation in D. mauritiana is associated with an insertion of DNA that is lost upon reversion to wild type, indicating that this DNA insert is in fact a transposable element. 相似文献
2.
Summary We transplanted pole cells betweenDrosophila melanogaster, D. mauritiana andD. ananassae to investigate the ability of germ cells to develop in the gonad of a heterospecific host, and to study the interaction between somatic follicle cells and the cells of the germ line in producing the species-specific chorion. FemaleD. mauritiana germ cells in aD. melanogaster ovary produced functional eggs with normal development potential. The same is true for the reciprocal combination. FemaleD. ananassae pole cells in aD. melanogaster host only developed to a very early stage and degenerated afterwards. None of the interspecific combinations of male pole cells led to functional sperm. We could not determine at what stage the transplanted male pole cells were arrested. The cooperation of follicle cells and the oocyte-nurse cell complex in producing the chorion was studied using the germ-line-dependent mutationfs(1) K10 ofD. melanogaster, which causes fused respiratory appendages and an abnormal chorion morphology. Wild-type femaleD. mauritiana germ cells in a mutantfs(1) K10 D. melanogaster ovary led to the production of wild-type eggs withD. melanogaster-specific, short respiratory appendages. On the other hand,D. melanogaster fs(1) K10 germ cells in aD. mauritiana ovary induced the formation of eggs with mutant fused appendages which were, however, typicallyD. mauritiana in length. When.D. mauritiana pole cells developed in aD. melanogaster ovary, the chorion exhibited a new imprint pattern that differs from both species-specific patterns. 相似文献
3.
Pedro Santamaria 《Development genes and evolution》1977,182(4):305-310
Summary The females produced in the crossesD. melanogaster×D. simulans andD. melanogaster×D. mauritiana are sterile and have reduced ovaries.Normal and fertile ovaries were produced when genetically marked pole cells ofD. melanogaster were transplanted into eggs which gave rise to the hybrid females.These results eliminate the possibility that the sterility of these hybrids is due to the somatic component, i.e. the follicular cells of the ovaries, or to other physiological causes. The results also suggest that the control of gonadal morphogenesis is dependent mainly on the germ line. 相似文献
4.
Defined mutant alleles with resident transposons display characteristic patterns of germinal and somatic reversion, and heritable
changes in the timing and frequency of reversions, which have been termed “change of state” by McClintock, constantly arise.
Several mechanisms were proposed to account for these changes. They may be ascribed to the structure and composition of the
elements themselves (composition hypothesis) or to their location (position hypothesis). In the current study, insertion positions
were determined for three autonomous En-controlled mutable alleles of the A2 locus in maize that show different somatic reversion patterns. A relationship was observed between En insertion positions in the single coding region of the intronless A2 gene and anthocyanin variegation patterns in the aleurone. An insertion in the 5′ region of the coding sequence produced
a very late somatic variegation pattern, whereas two early variegation patterns were caused by En insertions in the 3′ region of the coding sequence. 相似文献
5.
M. A. Voloshina Yu. A. Koromyslov O. V. Vaulin I. K. Zakharov 《Russian Journal of Genetics》2017,53(12):1299-1310
Mutations in the white locus emerged in highly mutable isofemale Drosophila melanogaster lines from the populations of Novosibirsk 2013 (NS3 line), Nalchik 2014 (N119 line), and Sakhalin Island 2014 (S46 line). A single white-eyed male found in the NS3 line was sterile. Phenotypically mutant derivatives (white gene alleles) differing in eye color (pure white, different shades of yellow (honey), orange (apricot), cherry, and red (wild type)) emerged during the N119 and S46 line breeding in the laboratory. Molecular genetic study of the structure of wild type white locus in initial lines and white-mutant derivatives de novo emerging from them, as well as other white lines from the fund of the Laboratory of Population Genetics of the Institute of Cytology and Genetics (Siberian Branch, Russian Academy of Sciences), was conducted. The pairs of primers flanking different white gene regions were selected. Six such pairs overlapped the coding part of the gene. Molecular genetic analysis demonstrated that most DNA defects were limited to the region which includes the first exon (34 lines). Among them, four mutant events were accompanied by an insertion of DNA fragments of approximately 800 bp; one mutation event was accompanied by a deletion of approximately 200 bp; in 29 cases, no PCR product was obtained (this can indicate that as a minimum one of the primer binding sites is damaged). The inserted DNA fragments have no homology with known D. melanogaster sequences presented in the NCBI database. The complete white gene deletion with the manifestation of mutant “white eyes” phenotype was registered in four cases (and only in the N119 line derivatives). Normal PCR product was obtained in 22 cases for all six DNA fragments. Among them, there are both alleles phenotypically mutant by the eye color (white, cherry, or orange) and revertants to the wild type (red). The abundance of defects in the beginning of the gene can indicate a multiplicity of mobile genetic element insertion sites in this part of the white gene in D. melanogaster. 相似文献
6.
7.
The pink-eyed unstable (p(un)) mutation is the result of a 70kb tandem duplication within the murine p gene. Homologous deletion/recombination of the locus to wild-type occurs spontaneously in embryos and results in pigmented spots in the fur and eye that persist for life. Such deletion events are also inducible by a variety of DNA damaging agents, as we have observed previously with the fur spot assay. Here, we describe the use of the retinal pigment epithelium (RPE) of the eye to detect reversion events induced with two differently acting agents. Benzo(a)pyrene (B(a)P) induces a high frequency, and X-ray exposure a more modest increase, of p(un) reversion in both the fur and the eye. The eye-spot assay requires fewer mice for significant results than the fur spot assay. Previous work had elucidated the cell proliferation pattern in the RPE and a position effect variegation phenotype in the pattern of p(un) reversions, which we have confirmed. Acute exposure to B(a)P or X-rays resulted in an increased frequency of reversion events. The majority of the spontaneous reversions lie toward the periphery of the RPE whereas induced events are found more centrally, closer to the optic nerve head. The induced distribution corresponds to the major sites of cell proliferation in the RPE at the time of exposure, and further advocates the proposal that dividing cells are at highest risk to develop deletions. 相似文献
8.
9.
Germ line transposition rates of the retrotransposon copia were directly measured in males and females of an inbred Drosophila melanogaster line, 2b3, which is highly polymorphic for copia insertion sites. The elevated germ line transposition rate of copia in this line (3–8 × 10−3 per generation per element) is confined to males, with transposition in females being undetectable under the conditions of
the experiment but at most 50-fold lower than the rate for males. To determine the molecular basis of this effect, copia RNA levels were measured in whole bodies and germ lines of male and female flies of both the unstable 2b3 line and a stable
line, Oregon RC-iso, which shows normal rates of copia transposition. Both male and female 2b3 flies contain much more copia RNA than flies of the stable line. However, 2b3 male germinal tissues contain much higher levels of copia RNA than the equivalent female tissues. The highest copia expression is detected in maturing primary spermatocytes. Our data show that high rates of germ line copia transposition are restricted to males by tissue-specific control of RNA levels and suggest that transposition of copia only occurs in fly tissues containing more than a relatively high threshold level of copia RNA.
Received: 8 October 1996 / Accepted: 7 January 1997 相似文献
10.
G. Gavazzi 《Molecular & general genetics : MGG》1967,99(2):151-164
Summary
R
st is an unstable allele of R that exhibits a mosaic phenotype in the aleurone, consisting of heavily pigmented spots on a colorless background. This variagated phenotype is presumably caused by the frequent somatic reversion of R from an inactive to an active form. Data are presented showing that such a reversion can take place at different times during the plant ontogenesis. Various stippled derivatives have been selected that differ in the number and size of dots formed in the endosperm. The reversion rate of R
st and derivatives toward self-colored (R
sc) has been estimated in the germinal and somatic tissues. This analysis indicates that some of the stippled derivatives differ in their capacity to revert toward R
sc in both the somatic and germ cells. The effect of internal factors on the R
st reversion rate has been measured. On the basis of these data the possible mechanism causing genetic instability of R
st are briefly discussed.This investigation has been partially supported by Consiglio Nazionale delle Ricerche, Roma. 相似文献
11.
Ayaka Taguchi Miki Tak ii Minako Motoishi Hidefumi Or ii Makoto Moch ii Kenji Watanabe 《Development, growth & differentiation》2012,54(8):767-776
Germ plasm is found in germ‐line cells of Xenopus and thought to include the determinant of primordial germ cells (PGCs). As mitochondria is abundant in germ plasm, vital staining of mitochondria was used to analyze the movement and function of germ plasm; however, its application was limited in early cleavage embryos. We made transgenic Xenopus, harboring enhanced green fluorescent protein (EGFP) fused to the mitochondria transport signal (Dria‐line). Germ plasm with EGFP‐labeled mitochondria was clearly distinguishable from the other cytoplasm, and retained mostly during one generation of germ‐line cells in Dria‐line females. Using the Dria‐line, we show that germ plasm is reorganized from near the cell membrane to the perinuclear space at St. 9, dependent on the microtubule system. 相似文献
12.
Shiraishi K Shimura T Taga M Uematsu N Gondo Y Ohtaki M Kominami R Niwa O 《Radiation research》2002,157(6):661-667
Untargeted mutation and delayed mutation are features of radiation-induced genomic instability and have been studied extensively in tissue culture cells. The mouse pink-eyed unstable (p(un)) mutation is due to an intragenic duplication of the pink-eyed dilution locus and frequently reverts back to the wild type in germ cells as well as in somatic cells. The reversion event can be detected in the retinal pigment epithelium as a cluster of pigmented cells (eye spot). We have investigated the reversion p(um) in F1 mice born to irradiated males. Spermatogonia-stage irradiation did not affect the frequency of the reversion in F1 mice. However, 6 Gy irradiation at the spermatozoa stage resulted in an approximately twofold increase in the number of eye spots in the retinal pigment epithelium of F1 mice. Somatic reversion occurred for the paternally derived p(un) alleles. In addition, the reversion also occurred for the maternally derived, unirradiated p(un) alleles at a frequency equal to that for the paternally derived allele. Detailed analyses of the number of pigmented cells per eye spot indicated that the frequency of reversion was persistently elevated during the proliferation cycle of the cells in the retinal pigment epithelium when the male parents were irradiated at the spermatozoa stage. The present study demonstrates the presence of a long-lasting memory of DNA damage and the persistent up-regulation of recombinogenic activity in the retinal pigment epithelium of the developing fetus. 相似文献
13.
Amalia Rosner Elizabeth Moiseeva Yuval Rinkevich Ziva Lapidot Baruch Rinkevich 《Developmental biology》2009,331(2):113-61
Germ cell sequestering in Animalia is enlightened by either, launching true germ line along epigenetic or preformistic modes of development, or by somatic embryogenesis, where no true germ line is set aside. The research on germ line-somatic tissue segregation is of special relevancy to colonial organisms like botryllid ascidians that reconstruct, on a weekly basis, completely new sets of male and female gonads in newly formed somatic tissues. By sequencing and evaluating expression patterns of BS-Vasa, the Botryllus schlosseri orthologue of Vasa, in sexually mature and asexual colonies during blastogenesis, we have demonstrated that the BS-Vasa mRNA and protein are not expressed exclusively in germ cell lineages, but appeared in cells repeatedly emerging de novo in the colony, independently of its sexual state. In addition, we recorded an immediate Vasa response to cellular stress (UV irradiation) indicating additional functions to its germ line assignments. To confirm germ lineage exclusivity, we examined the expression of three more stem cell markers (BS-Pl10, Bl-piwi and Oct4). Vasa co-expression with Pl10 and Oct4 was detected in germ line derivatives and with Bl-piwi in somatic tissues. Presumptive primordial germ cells (PGC-like cells), that are Vasa+/Pl10+/Oct4+ and 6-12 μm in diameter, were first detected in wrapped-tail embryos, in oozooids, in sexual/asexual colonies, within a newly identified PGC niche termed as ‘budlet niche’, and in circulating blood borne cells, indicating epigenetic embryogenesis. Alternatively, BS-Vasa co-expression with piwi orthologue, an omnipresent bona fide stemness flag, in non germ line cell populations, may indicate germ cell neogenesis (somatic embryogenesis) in B. schlosseri. Both alternatives are not necessarily mutually exclusive. 相似文献
14.
Loss of CMS-specific mitochondrial DNA arrangement in fertile segregants of Petunia hybrids 总被引:2,自引:0,他引:2
The progeny of somatic hybrid Petunia plants derived from the fusion of a male-fertile line and a cytoplasmic male-sterile (cms) line were examined. Male-fertile progeny derived from three different male-sterile somatic hybrid plants did not exhibit the mitochondrial DNA (mtDNA) arrangement which has previously been correlated with cms in Petunia. The cms-associated mtDNA arrangement was present in the male-sterile predecessors of these fertile revertants. Thus, it is concluded that the loss of this mtDNA arrangement is associated with reversion to fertility in the progeny of the unstable somatic hybrid petunia plants. 相似文献
15.
Using the newly isolated, germ line-dependent dominant female-sterile mutation Fs(1)K1237, we have characterized the germ line or somatic line dependence of 25 X-linked recessive female-sterile mutations. Since Fs(1)K1237/+ females fail to lay eggs, only germ line cells which lose Fs(1)K1237 as a result of X-ray-induced mitotic recombination are capable of producing eggs. Such recombination events will render genes on the homologous chromosome homozygous. If this chromosome carries a recessive female-sterile mutation, the fertility will be restored only if the altered function is not required in the germ line. Using this test, we have classified 25 recessive female-sterile mutations: 12 affect germ line function, 12 affect somatic line function, and one gave an ambiguous result for which an explanation is proposed. For a few of the somatic line-dependent mutants, we found that some eggs derived from germ line clones showed the same phenotype as eggs laid by females homozygous for the recessive female-sterile mutation. These results are discussed in terms of a coincident production of clones in the follicle cells. 相似文献
16.
Human Matrix Attachment Regions Insulate Transgene Expression from Chromosomal Position Effects in Drosophila melanogaster 总被引:6,自引:0,他引:6
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Stephanie J. Namciu Karen B. Blochlinger R. E. K. Fournier 《Molecular and cellular biology》1998,18(4):2382-2391
Germ line transformation of white− Drosophila embryos with P-element vectors containing white expression cassettes results in flies with different eye color phenotypes due to position effects at the sites of transgene insertion. These position effects can be cured by specific DNA elements, such as the Drosophila scs and scs′ elements, that have insulator activity in vivo. We have used this system to determine whether human matrix attachment regions (MARs) can function as insulator elements in vivo. Two different human MARs, from the apolipoprotein B and α1-antitrypsin loci, insulated white transgene expression from position effects in Drosophila melanogaster. Both elements reduced variability in transgene expression without enhancing levels of white gene expression. In contrast, expression of white transgenes containing human DNA segments without matrix-binding activity was highly variable in Drosophila transformants. These data indicate that human MARs can function as insulator elements in vivo. 相似文献
17.
Summary The deficiency of the 38B-40 region containing histone genes in one of the 2nd chromosomes of D. melanogaster triploid intersexes increases the template activity of X-chromosomes both in vivo and in vitro without noticeably affecting autosome activity. This deficiency in the heterozygous state inhibits the variegated position effect of the white gene in the T(1;3)w
vcotranslocation in diploid females and males, but not affect their rate of development. The variegation suppressor Su(var)hg-1 not only suppress the gene position effect in diploid flies, but also increases the template activity of X-chromosomes in triploid intersexes.The results are discussed with respect to the dependence of gene activity on the structure of chromosomes (density of DNP packing). 相似文献
18.
Maintenance of genome integrity is crucial for the germline, and this is reflected by lower mutation rates in gametes than somatic cells. Germ cells at different stages employ different DNA damage response (DDR) mechanisms. In response to certain DNA repair defects, primordial germ cells (PGCs) either undergo apoptosis or delayed proliferation, although little is known about the underlying mechanisms that govern these outcomes. Here, we report genetic studies of DDR pathways that underlie germ cell depletion in mice mutant for minichromosome maintenance 9 (Mcm9), a gene that plays a role in homologous recombination repair (HRR). Germ cell depletion in these mice is a result of reduced PGC numbers both before and after they arrive in the primitive gonads. This reduction was attributable to reduced proliferation, not apoptosis, and this response was independent of ATM‐CHK2‐TRP53‐P21 signaling. This mechanism of PGC depletion differs from that in Fancm mutants, which also display reduced PGC depletion that is partially orchestrated by the ATM‐TRP53‐P21 pathway. Germ cell depletion in mice doubly deficient for FANCM and MCM9 was additive, indicating that the damage caused by each mutation triggers different DDR pathways to slow the cell cycle as a means to preserve genomic integrity. genesis 53:678–684, 2015. © 2015 Wiley Periodicals, Inc. 相似文献
19.
J. F. Eisses D. Lafoe L.-A. Scott C. F. Weil 《Molecular & general genetics : MGG》1997,256(2):158-168
Plants form their gametes late in somatic development and, as a result, often pass somatic mutations on to their progeny.
Classic examples of this process are the germinal revertants of unstable, Ac/Ds transposon-induced kernel mutations in maize: frequent and early reversion events during somatic development are generally
correlated with a high frequency of revertant gametes. We have characterized a Ds allele of the maize waxy(wx) gene, wx-m5:CS7, for which the correlation between somatic and germinal reversion frequencies no longer holds. The ability of wx-m5:CS7 (CS7) to produce revertant gametes is suppressed ∼100-fold in comparison with a second Ds allele, wx-m5:CS8 (CS8), which has an identical insertion at Wx and the same frequent and early somatic reversion pattern in endosperm. The excision of Ds from wx is not reduced 100-fold in the somatic tissues of CS7 plants as compared with CS8 plants. Suppressed formation of CS7 revertant gametes is independent of the Ac transposase source and is heritably passed to the embryos of progeny kernels; however, frequent and early somatic reversion
is observed again in endosperms of these progeny kernels. This suppression appears to be caused by a dominant mutation in
a trans-acting product that can suppress the germinal reversion of other Ds-induced alleles as well; the mutation is tightly linked to Wx but is not in the CS7 Ds itself. Taken together, the data suggest a novel mode of developmental control of Ac/Ds elements by the host plant, suppressing element excision in the shoot meristem.
Received: 16 December 1996 / Accepted: 4 March 1997 相似文献
20.
L. A. Elkonin G. A. Gerashchenkov M. I. Tsvetova N. A. Rozhnova 《Russian Journal of Genetics》2010,46(7):808-818
Ethidium bromide treatment (15 mg/l, 26°C, 18 h) of a sorghum Zheltozernoe 10 callus culture yielded line Zh10-br1 displaying
multiple genetic instability. The line was characterized by a broad variety of mutations, which were identified in consecutive
generations obtained from one initial regenerant via self-pollination. The mutations caused male sterility (male sterility, generation R1), a low plant height (dwarfness, R2), a reduced awn length (awnless, R3), yellow leaves in seedlings (xantha, R6), leaf variegation (leaf variegation, R6), leaf bleaching (virescence, R6), etc. In some cases, segregation in families suggested a monogenic recessive inheritance for the induced mutations. Male
sterility was due to a range of defects that affected microsporogenesis and microgametogenesis and were probably caused by
mutations of several genes. Leaf variegation was due to the appearance of green sectors in originally albino seedling leaves;
the reversion occurred only in somatic tissues without affecting male and female gametes. In male-sterile and variegated mutants,
sequence-specific amplified polymorphism analysis with primers to the Isaak transposon revealed new DNA fragments, which were absent from the original line. The results supported the hypothesis that
the mutations isolated in line Zh10-br1 result from transposon mobilization induced by ethidium bromide and/or in vitro culture conditions. 相似文献