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1.
Summary The effects of acetic acid and specific growth rate on acetic acid tolerance and trehalose content of Saccharomyces cerevisiae CBS 2806 were studied using anaerobic chemostat cultures. Cells grown in the presence of acetic acid at a defined specific growth rate showed a higher acetic acid tolerance and a slightly lower trehalose content. Cells grown at a low specific growth rate showed a lower energy demand, a higher acetic acid tolerance, and a higher trehalose content. These results indicate that trehalose plays a growth rate dependent role in the tolerance of S. cerevisiae to acetic acid. 相似文献
2.
Dae Haeng Cho Soo-Jeong Shin Yong Hwan Kim 《Biotechnology and Bioprocess Engineering》2012,17(2):270-275
The effect of acetic acid and formic acid on acetone-butanol-ethanol (ABE) production by solventogenic Clostridia was investigated.
The ABE concentration in Clostridium acetobutylicum was found to have increased slightly on addition of 3.7 ∼ 9.7 g/L acetic acid, but was found to have drastically reduced
in the presence of 11.7 g/L acetic acid. However, the solvent production of C. beijerinckii was not affected by addition of acetic acid in the range of 3.7 ∼ 11.7 g/L. C. acetobutylicum was more vulnerable to formic acid than C. beijerinckii. In C. acetobutylicum, the total ABE production decreased to 77% on addition of 0.4 g/L formic acid and 25% with 1.0 g/L formic acid. The total
ABE production by C. acetobutylicum was also noted to have decreased from 15.1 to 8.6 g/L when 8.7 g/L acetic acid and 0.4 g/L formic acid co-existed. The solvent
production by C. beijerinckii was not affected at all under the tested concentration range of formic acid (0.0 ∼ 1.0 g/L) and co-presence of acetic acid
and formic acid. Therefore, C. beijerinckii is more favorable than C. acetobutylicum when the ABE is produced using lignocellulosic hydrolysate containing acetic and formic acid. 相似文献
3.
Shrimp farming accounts for more than 40% of the world shrimp production. Luminous vibriosis is a shrimp disease that causes
major economic losses in the shrimp industry as a result of massive shrimp kills due to infection. Some farms in the South
Asia use antibiotics to control Vibrio harveyi, a responsible pathogen for luminous vibriosis. However, the antibiotic-resistant strain was found recently in many shrimp
farms, which makes it necessary to develop alternative pathogen control methods. Short-chain fatty acids are metabolic products
of organisms, and they have been used as food preservatives for a long time. Organic acids are also commonly added in feeds
in animal husbandry, but not in aquaculture. In this study, growth inhibitory effects of short-chain fatty acids, namely formic
acid, acetic acid, propionic acid, and butyric acid, on V. harveyi were investigated. Among four acids, formic acid showed the strongest inhibitory effect followed by acetic acid, propionic
acid, and butyric acid. The minimum inhibitory concentration (MIC) of 0.035% formic acid suppressed growth of V. harveyi. The major inhibitory mechanism seems to be the pH effect of organic acids. The effective concentration 50 (EC50) values at 96 h inoculation for all organic acids were determined to be 0.023, 0.041, 0.03, and 0.066% for formic, acetic,
propionic, and butyric acid, respectively. The laboratory study results are encouraging to formulate shrimp feeds with organic
acids to control vibrio infection in shrimp aquaculture farms. 相似文献
4.
Summary The growth rate of the yeastCandida blankii in carbon-limited chemostat culture on a mixture of D-xylose and acetic acid as carbon sources was determined not only by the acetic acid concentration in the feed, but also by the ratio of xylose to acetic acid. The hypothesis is put forward that the inhibitory effect of acetic acid on the growth rate is determined, in part, by the specific rate of acetic acid metabolism. 相似文献
5.
Yahara GA Javier MA Tulio MJ Javier GR Guadalupe AU 《Bioprocess and biosystems engineering》2007,30(6):389-395
Glucose utilization by Brettanomyces bruxellensis at different acetic acid concentrations under aerobic and anaerobic conditions was investigated. The presence of the organic
acid disturbs the growth and fermentative activity of the yeast when its concentration exceeds 2 g l−1. A mathematical model is proposed for the kinetic behavior analysis of yeast growing in batch culture. A Matlab algorithm
was used for estimation of model parameters, whose confidence intervals were also calculated at a 0.95 probability level using
a t-Student distribution for f degrees of freedom. The model successfully simulated the batch kinetics observed at different concentrations of acetic acid
under both oxygen conditions. 相似文献
6.
Summary It is well known that Acetobacter is extremely sensitive in high total concentrations (GK)1 of ethanol and acetic acid. In the acetator, at a total concentration (GK) of 13%, ATP pool and growth show reverse behaviour. During the stationary, acidifying phase, the extracellular adenylate concentration amounts to 70% of the total edenylate pool (AN=ATP+ADP+AMP). In this range, the average value of the intracellular energy charge [EC=(ATP+1/2ADP)/(ATP+ADP+AMP)] is 0.82.After 45 s of interruption of aeration, the EC of the total culture dropped to a value of 0.58. After several weeks of storage, the EC of the inoculum amounted to 0.50. 相似文献
7.
Effects of acetic acid and lactic acid on the growth of Saccharomyces cerevisiae in a minimal medium 总被引:1,自引:0,他引:1
N V Narendranath K C Thomas W M Ingledew 《Journal of industrial microbiology & biotechnology》2001,26(3):171-177
Specific growth rates (μ) of two strains of Saccharomyces cerevisiae decreased exponentially (R
2>0.9) as the concentrations of acetic acid or lactic acid were increased in minimal media at 30°C. Moreover, the length of
the lag phase of each growth curve (h) increased exponentially as increasing concentrations of acetic or lactic acid were
added to the media. The minimum inhibitory concentration (MIC) of acetic acid for yeast growth was 0.6% w/v (100 mM) and that
of lactic acid was 2.5% w/v (278 mM) for both strains of yeast. However, acetic acid at concentrations as low as 0.05–0.1%
w/v and lactic acid at concentrations of 0.2–0.8% w/v begin to stress the yeasts as seen by reduced growth rates and decreased
rates of glucose consumption and ethanol production as the concentration of acetic or lactic acid in the media was raised.
In the presence of increasing acetic acid, all the glucose in the medium was eventually consumed even though the rates of
consumption differed. However, this was not observed in the presence of increasing lactic acid where glucose consumption was
extremely protracted even at a concentration of 0.6% w/v (66 mM). A response surface central composite design was used to
evaluate the interaction between acetic and lactic acids on the specific growth rate of both yeast strains at 30C. The data
were analysed using the General Linear Models (GLM) procedure. From the analysis, the interaction between acetic acid and
lactic acid was statistically significant (P≤0.001), i.e., the inhibitory effect of the two acids present together in a medium is highly synergistic. Journal of Industrial Microbiology & Biotechnology (2001) 26, 171–177.
Received 06 June 2000/ Accepted in revised form 21 September 2000 相似文献
8.
Yong Soo Park Kiyoshi Toda Masahiro Fukaya Hajime Okumura Yoshiya Kawamura 《Applied microbiology and biotechnology》1991,35(2):149-153
Summary The acetic acid concentration in a batch culture of Acetobacter aceti M23 increased up to 90 g/l by adding ethanol intermittently. Although the bacterial cells ceased growth at about 60 g acetic acid/l, non-viable cells still preserved ethanol oxidation activity. Cell recycling by filtration in a repeated fed-batch culture increased the overall acetic acid production rate 2.84-fold compared to that without cell recycling for the purpose of obtaining an acetic acid concentration of 80.8 g/l. Repeated fed-batch cultivation with cell recycle was effective for increasing the production rate of acetic acid and obtaining high amounts close to a lethal concentration (90 g/l).Offprint requests to: Kiyoshi Toda 相似文献
9.
Lina Lindahl Samuel Genheden Leif A. Eriksson Lisbeth Olsson Maurizio Bettiga 《Biotechnology and bioengineering》2016,113(4):744-753
Lignocellulosic raw material plays a crucial role in the development of sustainable processes for the production of fuels and chemicals. Weak acids such as acetic acid and formic acid are troublesome inhibitors restricting efficient microbial conversion of the biomass to desired products. To improve our understanding of weak acid inhibition and to identify engineering strategies to reduce acetic acid toxicity, the highly acetic‐acid‐tolerant yeast Zygosaccharomyces bailii was studied. The impact of acetic acid membrane permeability on acetic acid tolerance in Z. bailii was investigated with particular focus on how the previously demonstrated high sphingolipid content in the plasma membrane influences acetic acid tolerance and membrane permeability. Through molecular dynamics simulations, we concluded that membranes with a high content of sphingolipids are thicker and more dense, increasing the free energy barrier for the permeation of acetic acid through the membrane. Z. bailii cultured with the drug myriocin, known to decrease cellular sphingolipid levels, exhibited significant growth inhibition in the presence of acetic acid, while growth in medium without acetic acid was unaffected by the myriocin addition. Furthermore, following an acetic acid pulse, the intracellular pH decreased more in myriocin‐treated cells than in control cells. This indicates a higher inflow rate of acetic acid and confirms that the reduction in growth of cells cultured with myriocin in the medium with acetic acid was due to an increase in membrane permeability, thereby demonstrating the importance of a high fraction of sphingolipids in the membrane of Z. bailii to facilitate acetic acid resistance; a property potentially transferable to desired production organisms suffering from weak acid stress. Biotechnol. Bioeng. 2016;113: 744–753. © 2015 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. 相似文献
10.
11.
Rogério S. Pereira Solange I. Mussatto Inês C. Roberto 《Journal of industrial microbiology & biotechnology》2011,38(1):71-78
The inhibitory action of acetic acid, ferulic acid, and syringaldehyde on metabolism of Candida guilliermondii yeast during xylose to xylitol bioconversion was evaluated. Assays were performed in buffered and nonbuffered semidefined
medium containing xylose as main sugar (80.0 g/l), supplemented or not with acetic acid (0.8–2.6 g/l), ferulic acid (0.2–0.6 g/l),
and/or syringaldehyde (0.3–0.8 g/l), according to a 23 full factorial design. Since only individual effects of the variables were observed, assays were performed in a next step
in semidefined medium containing different concentrations of each toxic compound individually, for better understanding of
their maximum concentration that can be present in the fermentation medium without affecting yeast metabolism. It was concluded
that acetic acid, ferulic acid, and syringaldehyde are compounds that may affect Candida guilliermondii metabolism (mainly cell growth) during bioconversion of xylose to xylitol. Such results are of interest and reveal that complete
removal of toxic compounds from the fermentation medium is not necessary to obtain efficient conversion of xylose to xylitol
by Candida guilliermondii. Fermentation in buffered medium was also considered as an alternative to overcome the inhibition caused by these toxic compounds,
mainly by acetic acid. 相似文献
12.
The possible interaction of the plant hormones auxin and ethylene and the role of 1-aminocyclopropane-1-carboxylate (ACC)
deaminase containing bacteria on ethylene production in canola (Brassica campestris) in the presence of inhibitory concentrations of growth regulators were investigated. The effects of auxin (indole-3-acetic
acid and 2,4-dichlorophenoxy acetic acid), auxin transport inhibitor 2-(p-chlorophenoxy)-2-methylpropionic acid, ethylene precursor 1-aminocyclopropane-1-carboxylate and ethylene synthesis inhibitor
l-α-(2-aminoethoxyvinyl)glycine hydrochloride on root elongation were concentration dependent. Exogenous addition of growth
regulators influences the enzyme activities of ethylene production and we have presented here evidences that support the hypothesis
that inhibitory effects of auxin on root elongation are independent of ethylene. Additionally, we have proved that inoculation
of ACC deaminase containing Methylobacterium
oryzae sequester ACC exuded from roots and hydrolyze them lowering the concentration of ACC in root exudates. However, the inhibitory
actions of exogenous additions of auxins could not be ameliorated by bacterial inoculation that reduces ethylene concentration
in canola seedlings. 相似文献
13.
Correlation between acetic acid resistance and characteristics of PQQ-dependent ADH in acetic acid bacteria 总被引:7,自引:0,他引:7
Trcek J Toyama H Czuba J Misiewicz A Matsushita K 《Applied microbiology and biotechnology》2006,70(3):366-373
In this study, we compared the growth properties and molecular characteristics of pyrroloquinoline quinone (PQQ)-dependent alcohol dehydrogenase (ADH) among highly acetic acid-resistant strains of acetic acid bacteria. Ga. europaeus exhibited the highest resistance to acetic acid (10%), whereas Ga. intermedius and Acetobacter pasteurianus resisted up to 6% of acetic acid. In media with different concentrations of acetic acid, the maximal acetic acid production rate of Ga. europaeus slowly increased, but specific growth rates decreased concomitant with increased concentration of acetic acid in medium. The lag phase of A. pasteurianus was twice and four times longer in comparison to the lag phases of Ga. europaeus and Ga. intermedius, respectively. PQQ-dependent ADH activity was twice as high in Ga. europaeus and Ga. intermedius as in A. pasteurinus. The purified enzymes showed almost the same specific activity to each other, but in the presence of acetic acid, the enzyme activity decreased faster in A. pasteurianus and Ga. intermedius than in Ga. europaeus. These results suggest that high ADH activity in the Ga. europaeus cells and high acetic acid stability of the purified enzyme represent two of the unique features that enable this species to grow and stay metabolically active at extremely high concentrations of acetic acid. 相似文献
14.
On the concentration of acetic acid in straw and soil 总被引:5,自引:0,他引:5
Summary Freshly harvested wheat straw contained 0.096 g water g–1 dry straw and 180 mM acetic acid. The straw absorbed water more rapidly from wet soil. The concentration of acetic acid fell to about 10 mM within 6 h of incorporation of straw in the soil and then remained relatively constant for a period of 12 days, irrespective of soil moisture content. In soil at its maximum water holding capacity after gravitational drainage, the decline in acetic acid concentration (c) with distance (d) from wheat or barley straw was exponential, with c=co e–nd where co is the concentration of acetic acid at the straw surface and n is a constant (0.46 for barley and 0.42 for wheat straw). The presence of acetic acid seems to be a major cause of poor establishment and growth when seeds and seedling roots come into contact with straw. 相似文献
15.
J. C. du Preez M. Immelman J. L. F. Kock S. G. Kilian 《World journal of microbiology & biotechnology》1997,13(1):81-87
The effect of different initial acetic acid concentrations on the growth of and lipid and gamma-linolenic acid (GLA) production
byMucor circinelloides CBS 203.28 was determined in a 14 litre stirred tank reactor operated in a fedbatch, pH-stat mode with acetic acid as carbon
source and pH titrant. Increased acetic acid concentrations in the culture resulted in a significant increase in the crude
oil content of the biomass. By contrast, all the other parameters such as the biomass concentration, GLA and oil yield on
acetic acid, the GLA content of the biomass and oil, the growth rate and volumetric rate of GLA production decreased with
an increase in acetic acid concentration. The best results were obtained with acetic acid at 2 g/1, which gave 39.8 mg GLA/g
biomass and 15.6% GLA in the neutral lipid fraction, amounting to 340 mg GLA/1 culture. A decrease in the glyco- and phospho-lipid
fractions during the cultivation coincided with an increase in the neutral lipid fraction. The GLA content of the biomass
remained within rather narrow limits of 3.5% to 4% of the biomass, irrespective of the oil content of the biomass. The fatty
acid profile was not greatly affected by the acetic acid concentration. The hyphae of the fungus were characterized by the
accumulation of large intracellular oil droplets and some septa delimited the hyphae. 相似文献
16.
17.
The FPS1 gene coding for the Fps1p aquaglyceroporin protein of an industrial strain of Saccharomyces cerevisiae was disrupted by inserting CUP1 gene. Wild-type strain, CE25, could only grow on YPD medium containing less than 0.45% (v/v) acetic acid, while recombinant
strain T12 with FPS1 disruption could grow on YPD medium with 0.6% (v/v) acetic acid. Under 0.4% (v/v) acetic acid stress (pH 4.26), ethanol production
and cell growth rates of T12 were 1.7 ± 0.1 and 0.061 ± 0.003 g/l h, while those of CE25 were 1.2 ± 0.1 and 0.048 ± 0.003 g/l h,
respectively. FPS1 gene disruption in an industrial ethanologenic yeast thus increases cell growth and ethanol yield under acetic acid stress,
which suggests the potential utility of FPS1 gene disruption for bioethanol production from renewable resources such as lignocelluloses. 相似文献
18.
Georgia Pacheco Rachel Fatima Gagliardi Leonardo Alves Carneiro José Francisco Montenegro Valls Elisabeth Mansur 《In vitro cellular & developmental biology. Plant》2008,44(1):14-17
Seed explants of A. stenosperma were cultured on MS medium supplemented with 6-benzylaminopurine with the aim of rescuing nonviable accessions stored in
seed bank conditions. The regeneration potential of leaf explants from in vitro plants derived from embryonic axes was studied by using whole leaflets and leaflet segments. Explants were cultured on Murashige
and Skoog (MS) medium supplemented with different concentrations of 6-benzylaminopurine and naphthalene acetic acid. Indirect
organogenesis was observed in response to 6-benzylaminopurine, either alone or in association with naphthalene acetic acid,
in both explant types. Media supplemented with naphthalene acetic acid as the sole growth regulator induced rhizogenesis in
whole leaflets and leaflet segments, with subsequent shoot production directly from the roots. 相似文献
19.
Dao-Qiong Zheng Xue-Chang Wu Pin-Mei Wang Xiao-Qin Chi Xiang-Lin Tao Ping Li Xin-Hang Jiang Yu-Hua Zhao 《Journal of industrial microbiology & biotechnology》2011,38(3):415-422
Acetic acid existing in a culture medium is one of the most limiting constraints in yeast growth and viability during ethanol
fermentation. To improve acetic acid tolerance in Saccharomyces cerevisiae strains, a drug resistance marker-aided genome shuffling approach with higher screen efficiency of shuffled mutants was developed
in this work. Through two rounds of genome shuffling of ultraviolet mutants derived from the original strain 308, we obtained
a shuffled strain YZ2, which shows significantly faster growth and higher cell viability under acetic acid stress. Ethanol
production of YZ2 (within 60 h) was 21.6% higher than that of 308 when 0.5% (v/v) acetic acid was added to fermentation medium.
Membrane integrity, higher in vivo activity of the H+-ATPase, and lower oxidative damage after acetic acid treatment are the possible reasons for the acetic acid-tolerance phenotype
of YZ2. These results indicated that this novel genome shuffling approach is powerful to rapidly improve the complex traits
of industrial yeast strains. 相似文献
20.
Graves T Narendranath NV Dawson K Power R 《Applied microbiology and biotechnology》2007,73(5):1190-1196
The combined effects of lactic acid and acetic acid on ethanol production by S. cerevisiae in corn mash, as influenced by temperature, were examined. Duplicate full factorial experiments (three lactic acid concentrations
× three acetic acid concentrations) were performed to evaluate the interaction between lactic and acetic acids on the ethanol
production of yeast at each of the three temperatures, 30, 34, and 37°C. Corn mash at 30% dry solids adjusted to pH 4 after
lactic and acetic acid addition was used as the substrate. Ethanol production rates and final ethanol concentrations decreased
(P<0.001) progressively as the concentration of combined lactic and acetic acids in the corn mash increased and the temperature
was raised from 30 to 37°C. At 30°C, essentially no ethanol was produced after 96 h when 0.5% w/v acetic acid was present
in the mash (with 0.5, 2, and 4% w/v lactic acid). At 34 and 37°C, the final concentrations of ethanol produced by the yeast
were noticeably reduced by the presence of 0.3% w/v acetic acid and ≥2% w/v lactic acid. It can be concluded that, as in previous
studies with defined media, lactic acid and acetic acid act synergistically to reduce ethanol production by yeast in corn
mash. In addition, the inhibitory effects of combined lactic and acetic acid in corn mash were more apparent at elevated temperatures. 相似文献