Effect of the antioxidant dibunol and its combination with phenazepam on the behavior of rats in a conflict situation

The influence of dibunol, phenazepam used alone and combined on rat conflict behavior and rat blood and brain malonic dialdehyde content was studied. It was shown that dibunol exerts an unmarked anticonflict action that can be removed by bicuculline. Combined administration of dibunol and phenazepam potentiates appreciably the anticonflict effect. This permits reducing the doses of the drugs. The anxiolytic effect of dibunol alone and combined with phenazepam is attended by a decrease in the content of malonic dialdehyde in rat blood and brain, evidence of the reduction of the lipid peroxidation intensity.     

New paths in the pathogenetic correction of hemic hypoxia]

It is stated that prophylactic administration of ional (dibunol) and taurine to rats exerts an antihypoxic effect in case of acute hemic hypoxia. It is expressed in a decrease of methemoglobin level in blood, increase of pO2, in the skeletal muscles, normalization of the structure of hematoparenchymatous barriers, prevention or decrease in a fall of the rate of oxygen consumption by tissues.     

Effect of the antioxidant dibunol on the electron paramagnetic resonance signals in the tissues of rats of different ages

Changes in the intensity of EPR signals from the skeletal and heart muscles, liver, kidneys, thyroid, adrenal cortex, and blood were studied in relation to the animals age and the effect of the antioxidant dibunol. Essential changes were revealed in metabolic activity of the majority of the tissues under study as early as within the first 6 hours after antioxidant administration.     

House-dust mites allergen for specific immunotherapy by oral administration: experimental study

The oral medicinal form of house-dust allergen has been prepared with the use of new technology. To produce the preparation, the standard water-saline extract obtained from house-dust mites (Dermatophagoides farinae) allergen has been used. This preparation is chemically stable and does not lose its biological activity for 2 - 3 years of storage. The microbiological purity of the allergen meets the requirements for oral preparations. The allergen has proved to be safe in cases of prolonged administration and has no chronic toxicity. As shown with the use of immunochemical methods, the preparation has high immunogenicity and lower allergenicity. This preparation may be recommended for clinical trials on a limited circle of subjects.     

Limited area of coronary-occlusion myocardial infarct in rats undergoing antioxidant therapy

The synthetic antioxidant dibunol, (ionol. 2,6-ditret-butyl-4-methylphenol) produces the limitation of the zone of the coronaro-occlusion myocardial infarction in rats by 15.8 and 24.2% on day 7 during daily oral administration in doses of 80 and 120 mg/kg, respectively. In the doses used, dibunol reduces the activity of glutathione peroxidase but does not change the activity of glutathione-S-transferase and superoxide dismutase in the infarction zone of the myocardium. It is concluded that free radical products play an important role in ischemic and infarction damage to the myocardium.     

Antioxidant ionol suppresses ACTH-dependent secretion of corticosterone

It has been shown that the preliminary administration of a synthetic antioxidant ionol (dibunol, BHT) prevented to a considerable extent the increase in the concentration of corticosterone in the adrenal glands and blood plasma induced by ACTH administration or emotional-painful stress.     

Significance of sarcolemma damage in the pathogenesis of myocardial ischemia induced by pituitrin-isadrin and its correction using the antioxidant dibunol

The elevation of cardiomyocyte membrane permeability has been demonstrated during pituitrin-isadrin-induced myocardial ischemia. Preventive 7-day oral administration of an antioxidant dibunol (30 and 120 mg/kg) preserved sarcolemmal integrity, decreased myocardial membrane permeability to sulfacetamide sodium, and reduced peroxide and mechanical erythrocyte hemolysis. Inhibition of lipid peroxidation with an antioxidant dibunol improved myocardial injury and decreased the death rate of animals with catecholamine-induced myocardial ischemia. These data suggest the involvement of lipid peroxidation in the development of ischemic myocardial injury.     

Radiosensitizing activity and metabolism of nitrofuran derivatives. Preparation M-106

It was shown that the preparation of a nitrofuran M-106 series exerts a radiosensitizing effect on tumors with contact application. The observed effect is practically absent after noncontact administration of the preparation. On the basis of the data obtained from studies of M-106 metabolism in microsomes of liver and Ehrlich ascites tumor cells of mice it is concluded that the intact form of the preparation is responsible for the radiosensitizing effect, and that the absence of this effect with the noncontact administration is due to its low concentration in the tumor because of the active metabolic transformation of M-106 in the animal liver.     

Effect of dibunol and isoptin on the creatine kinase and myoglobin content of the blood serum in dogs undergoing postischemic coronary reperfusion

The effect of an antioxidant dibunol and calcium antagonist verapamil on postperfusion release of myoglobin (Mb) and MB-creatine kinase (MB-CK) has been assessed in 30 dogs with experimental coronary occlusive myocardial infarction. It has been shown that reperfusion after 3-hour ischemia does not only accelerate the release of intracellular proteins, but also leads to pronounced myoglobinemia and blood enzymes. In postischemic blood flow recovery with combined dibunol and verapamil preliminary injections, an almost threefold decrease in MB-CK and Mb blood content, as compared to "reperfusion" indexes, was observed by the 10th minute of reperfusion.     

Study of the pharmacological properties and safety of a solution of levomycetin in hexamethylene tetramine for intravenous administration]

Pharmacological properties of 2 per cent levomycetin solution in 40 percent hexamethylentetramine solution, as a new pharmaceutical form of levomycetin for intravenous administration prepared at drug-stores were studied. The maximum tolerating doses of the drug for mice, rabbits, and dogs were 26-47 times higher than the therapeutic ones with respect to the content of levomycetin and hexamethylentetramine. No increase in the toxicity of levomycetin and hexamethylentetramine in the preparation was observed. The drug in the doses 16 times higher than the therapeutic ones by the content of levomycetin did not almost change the arterial pressure and the drug in the doses 3.7 times higher than the therapeutic ones did not affect the blood coagulation either in acute experiments, or on its prolong intravenous infusion. Repeated administrations of the drug to rats and rabbits for 15-18 days in doses 3.7-4.8 times higher than the therapeutic ones by the content of levomycetin were innocuous for the animals. Absorption, circulation in the blood, distribution in the tissues and excretion with the urine of levomycetin used in the above pharmaceutical form did not differ from circulation of the antibiotic on its intravenous and oral administration. The drug is recommended for use in medical practice.     

Evaluation on monkeys of reactogenicity and effectiveness of the complex immunoglobulin preparation formulation

Experiments on 75 monkeys, 20--healthy and 75--with diarrhea, showed that ready medicinal forms of the complex immunoglobulin preparation in tablets, capsules or in a dried form (vials) were safe, well tolerated, clinically and bacteriologically effective. When used simultaneously with antiparasitic treatment, this preparation, irrespective of the kind of its ready medicinal form, showed high curative effect (80-90%). The diarrhea ceasing was accompanied by the gradual normalization of intestinal microbiocenosis (an increased concentrations of Escherichia coli and Bifidobacterium, a decreased amount of opportunistic bacteria). The complex method of the treatment of diarrhea in monkeys (antibiotics, trichopol, phage and probiotics) made it possible to achieve curative effect only in 60% of cases, which required the additional course of treatment, namely the use of rehydration therapy.     

Effect of chronic alcoholism on the state of the microcirculatory bed of the myocardium

Ultrastructure of myocardial capillaries of rats was studied in cases of chronic alcohol intoxication, experimental alcoholic cardiomyopathy (ACM) and its correction with antioxidants (vitamin E, dibunol). Alterations in the microcirculatory bed were similar in all groups of animals irrespective of disturbances in cardiomyocytes. Cardiomyocyte ultrastructure was improved after treatment with antioxidants, but capillary bed was the same as in untreated animals. High dibunol doses caused the onset of perivascular sclerosis. Disturbances in the microcirculatory bed are, probably, the first step in the determination of ACM pathogenesis and therapy of ACM must be directed at the correction of both alterations of cardiomyocytes and capillary bed.     

Acridonacetic acid: pharmacological properties and clinical use

The summarized data on the biological activity of acridoneacetic acid, a low-molecular interferon inducer are presented: the amount and type of induced interferon, producer cells, interaction with viruses; the antiviral, antitumoral and anti-inflammatory activity of the preparation has been demonstrated. The specific features of interferon induction by virus (a biological inducer), in contrast to acridoneacetic acid (a chemical inducer) have been noted. The possible mechanisms of interferon induction by low-molecular interferonogen are considered. A number of interferonogens, salts of acridoneacetic acid, proposed as medicinal preparations are considered. The results of the clinical use of Cycloferon, a medicinal form of acridoneacetic acid, are presented.     

A review on ethno-medicinal plants used in traditional medicine in the Kingdom of Saudi Arabia

The traditional medicine based on medicinal plants in the Kingdom of Arabia Saudia presents a strong relationship belonging to natural remedies, health, diet, and folk healing practice recognized by a specific culture. The aim of the current study is to carry out an ethnobotanical review on medicinal plants used in traditional medicine in the Kingdom of Arabia Saudia including information on plant species, used parts, preparation method as well as medical uses. Earlier published data in journals, textbooks, periodicals, websites, and databases written in pharmacological evidence of Suadi medicinal plants were based on gathering information. The present review work reported that 96 species belonging to 47 families have been used in Saudi Pharmacopeia. Amaranthaceae has the highest number of plant species (7) Followed by Asteraceae, Apocynaceae, and Fabaceae with 5 plant species in each. The inventoried plant species in the current work are frequently used for the treatment of various illnesses and to ensure the medication safety of Saudi people. The biological analysis of plant form used in Saudi natural remedies showed the dominance of herb and subshrub form with a percentage of 43% and 30% respectively. The most used preparation method of plant drugs, which used in Saudi Alternative medicine was decoction and infusion. The whole plant, leaves, seeds, and aerial parts were the most useful plant parts in natural preparation in Saudi traditional medicine with a percentage of 29%, 28%, 7%, and 5% respectively as reported in the present review work. The present review work gives big data about medicinal plants used in traditional medicine in the Kingdom of Saudi Arabia including data about plant species, used parts, preparation method as well as medical uses.     

Effect of stress and the antioxidant ionol on the biosynthesis of catecholamines and the content of dopamine in the heart and adrenal glands

The effects of a synthetic antioxidant ionol (dibunol) on the biosynthesis and content of catecholamines in the heart and adrenal glands were studied. It was shown that in stress a mobilization of catecholamine reserve is combined with a considerable increase in dopamine concentration. In conditions of physiological rest, ionol did not affect the studied indices of adrenal catecholamine biosynthesis, while in the heart it enhanced the dopamine synthesis and content. With ionol administration, stress did not suppress but, on the contrary, increased the neuronal uptake and noradrenaline biosynthesis, catecholamine concentration remaining practically unchanged. Simultaneously, a manyfold increase in the biosynthesis along with a considerable increase in the concentration of dopamine developed in both organs. The data obtained suggest that ionol realizes its stress-defensive effect to a great extent due to the activation of catecholamine biosynthesis and to a concomitant increase in dopamine accumulation.     

Traditional usage of medicinal plants among the local communities of Peshawar valley,Pakistan

Peshawar has a miscellaneous range of population that consequently brought their traditional knowledge of plants to the valley. Aims of the study were to report and document the traditional knowledge of medicinal plants resources of the Peshawar valley. The main objective of the study was to assess and document the ethnobotanical uses of medicinal plants in the study area. Data collection was carried out from March 2017 to December 2017. About 250 informants of different ages were interviewed; most of them were aged people between 60 and 70 years. The interviews were conducted using structured questioner composed of various questions about the ethnomedicinal uses of plants. The data was quantitatively analyzed using quantitative indices like frequency citation FC, use value (UV), use report (UR), relative frequency citation (RFC) Fidelity level (FL) and Jaccard index (JI). A comparison with previous ethnomedicinal studies in order to report some novel uses. A total of 71 plants species belonging to 59 genera and 31 families were being used for various ailment treatments. Out of 31 families, Asteraceae and Amaranthaceae (7 species each) were found the dominant families. Regarding plant habit, herbs 63% were the leading growth form. Leaves were the most frequently used part of the plant in the preparation of medicines. The most frequent mode of preparation was decoction while the oral route of administration was the preferred mode of administration. A review of the active compounds of the reported plants was also documented to authenticate the data. Medicinal plants for high ranked diseases to be to screen for further ethnopharmacological and phytochemical studies. The medicinal flora of the valley is facing improper collection, overgrazing, browsing, deforestation, industrialization, construction of roads and buildings. Therefore proper conservation strategies may be adapted to promote the sustainable use of medicinal plants.     

Mechanism of the antimutagenic action of dibunol

In experiments with cultured V-79 Chinese hamster cells a study was made of the influence of dibunol on the level of gamma-radiation-induced micronuclei and sedimentation properties of cell nucleoids during ultracentrifugation in a neutral sucrose gradient. Protection of DNA molecule against the effects of both primary and secondary damages was found to be involved in the mechanism of the protective action of dibunol.     

Plutonium-239 distribution in the body in relation to the rhythm of administration of an iron preparation

A study was made of the dependence of plutonium distribution among organs and tissues on the time of administration of iron preparation. Iron decreased the share of plutonium in bone tissues, somewhat increased it in soft tissues, and enhanced the excretion of the radionuclide from the organism. The protective efficiency of the preparation in relation to bone tissue was associated with the time of the administration thereof.     

Effect of synthetic phenol antioxidants on the parietal pH value of the gastrointestinal tract in intact and vagotomized rats

The influence of synthetic phenol antioxidants dibunol and BH-3 on the value of parietal pH has been studied in the stomach, duodenum, jejunum, ileum, caecum and rectum of intact and vagotomized rats 1, 7, 14, 30 and 60 days after treatment. It was shown that vagotomy leads to an increase in the stomach pH and to a decrease in pH in other parts of the gastrointestinal tract. Maximum BH-3 doses lead to pH increase in the stomach of intact rats. Chronic administration of therapeutic drug doses does not change pH value in control and vagotomized animals.