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1.
Monoclonal antibody characterisation of slime sheath: the extracellular matrix of Dictyostelium discoideum 总被引:4,自引:0,他引:4
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Proteins can be extracted from the slime sheath of Dictyostelium discoideum slugs by denaturing agents. A subset of these proteins is also released by cellulase digestion of the sheath, implying that protein-protein and protein-cellulose interactions are involved in sheath protein retention. It seems probable that the cellulose-associated sheath proteins are also associated with the cellulose of mature stalk cells. Monoclonal antibodies directed against sheath demonstrate extensive sharing of antigenic determinants between sheath proteins and a limited degree of antigenic sharing between sheath and slug cell proteins. All of the proteins recognised by these monoclonal antibodies are developmentally regulated. These results are discussed in terms of the structure of the sheath and its possible role(s) in D. discoideum development. 相似文献
2.
Maria G. Abad Yicheng Long R. Dimitri Kinchen Elinor T. Schindel Michael W. Gray Jane E. Jackman 《The Journal of biological chemistry》2014,289(22):15155-15165
Mitochondrial tRNA (mt-tRNA) 5′-editing was first described more than 20 years ago; however, the first candidates for 5′-editing enzymes were only recently identified in a eukaryotic microbe (protist), the slime mold Dictyostelium discoideum. In this organism, eight of 18 mt-tRNAs are predicted to be edited based on the presence of genomically encoded mismatched nucleotides in their aminoacyl-acceptor stem sequences. Here, we demonstrate that mt-tRNA 5′-editing occurs at all predicted sites in D. discoideum as evidenced by changes in the sequences of isolated mt-tRNAs compared with the expected sequences encoded by the mitochondrial genome. We also identify two previously unpredicted editing events in which G-U base pairs are edited in the absence of any other genomically encoded mismatches. A comparison of 5′-editing in D. discoideum with 5′-editing in another slime mold, Polysphondylium pallidum, suggests organism-specific idiosyncrasies in the treatment of U-G/G-U pairs. In vitro activities of putative D. discoideum editing enzymes are consistent with the observed editing reactions and suggest an overall lack of tRNA substrate specificity exhibited by the repair component of the editing enzyme. Although the presence of terminal mismatches in mt-tRNA sequences is highly predictive of the occurrence of mt-tRNA 5′-editing, the variability in treatment of U-G/G-U base pairs observed here indicates that direct experimental evidence of 5′-editing must be obtained to understand the complete spectrum of mt-tRNA editing events in any species. 相似文献
3.
Sawarkar R Roy N Rao S Raman S Venketesh S Suguna K Tatu U 《Journal of molecular biology》2008,383(1):24-35
Cytosolic heat shock protein 90 (Hsp90) has been implicated in diverse biological processes such as protein folding, cell cycle control, signal transduction, development, and morphological evolution. Model systems available for studying Hsp90 function either allow ease of manipulation for biochemical studies or facilitate a phenomenological study of its role in influencing phenotype. In this work, we have explored the use of the cellular slime mold Dictyostelium discoideum to examine cellular functions of Hsp90 in relation to its multicellular development. In addition to cloning, purification, biochemical characterization, and examination of its crystal structure, our studies, using a pharmacological inhibitor of Hsp90, demonstrate a role for the cytoplasmic isoform (HspD) in D. discoideum development. Inhibition of HspD function using geldanamycin (GA) resulted in delayed aggregation and arrest of D. discoideum development at the ‘mound’ stage. Crystal structure of the amino-terminal domain of HspD showed a binding pocket similar to that described for yeast Hsp90. Fluorescence spectroscopy, as well as GA-coupled beads affinity pulldown, confirmed a specific interaction between HspD and GA. The results presented here provide an important insight into the function of HspD in D. discoideum development and emphasize the potential of the cellular slime mold to serve as an effective model for studying the many roles of Hsp90 at cellular and organismal levels. 相似文献
4.
Control of phototactic migration in Dictyostelium discoideum 总被引:4,自引:0,他引:4
Phototactic migration of pseudoplasmodia of the cellular slime mold, Dictyostelium discoideum, is directed by a response at the anterior tip. Horizontal light appears to be focused by refraction at the surface of the pseudoplasmodium such that it acts preferentially on the distal cells. We have been able to show that light stimulates the rate of pseudoplasmodial movement up to 80%. This increase is dependent on the intensity of the incident light. Thus it appears that light can control the direction of migration by increasing the rate of movement on the distal side. The anterior cells are then turned toward the light by cohesion to the more slowly moving proximal side. Migration rate in the dark may be limited by the rate of synthesis or deposition of the surface sheath surrounding the pseudoplasmodium. It is suggested that light increases the rate of migration by stimulating the formation of the surface sheath. Localized stimulation would then result in a turning response. 相似文献
5.
Blacklock BJ Kelley D Patel S 《Biochemical and biophysical research communications》2008,374(2):226-230
Fatty acid elongation was examined in the cellular slime mold, Dictyostelium discoideum. Profiling of the total fatty acid content of D. discoideum indicated that fatty acid elongation is active. Orthologs of the fatty acid elongase ELO family were identified in the D. discoideum genome and the cDNA for one, eloA, was cloned and functionally characterized by expression in yeast. EloA is a highly active ELO with strict substrate specificity for monounsaturated fatty acids, in particular 16:1Δ9 to produce the unusual 18:1Δ11 fatty acid. This is the first report on fatty acid elongation in a cellular slime mold. 相似文献
6.
The Dictyostelium discoideum lectins, discoidin I and discoidin II, and the endogenous ligands to which they bind were immunohistochemically localized in sections of this organism at successive stages of development. For these studies, an axenic strain, AX3, was grown in a macromolecule-depleted medium rather than on bacteria, which themselves contain discoidin-binding ligands. Discoidin I-binding sites (endogenous ligands) in sections of D. discoideum were concentrated in the slime coat around aggregates, whereas discoidin II-binding sites were observed in a vesicle-like distribution in prespore cells and also in spore coats. In contrast, discoidin II did not bind to the slime coat and discoidin I bound relatively poorly to prespore cells and spore coats. The distributions of the endogenous lectins themselves were the same in axenically grown cells as previously reported for cells raised on bacteria. Discoidin I was concentrated in the slime coat and around stalk cells, and discoidin II was prominent in and around prespore cells. The congruent localization of each lectin with its endogenous ligand suggests that discoidin I normally functions in association with glycoconjugates in the slime around aggregates, and discoidin II with the galactose-rich spore coat polysaccharide. 相似文献
7.
An ultrastructural study has been made of the life cycle of the cellular slime mold Dictyostelium minutum. The development of D. minutum is rather simple if compared with Dictyostelium discoideum. After 2 hr of starvation, amoebas move in a nonpulsatile manner towards an acrasin-secreting founder cell. The chemotactic signal is not relayed by the amoebas and stream formation toward primary aggregation centers does not occur. Usually, more than one fruiting body arises from one pseudoplasmodium. No migration of the pseudoplasmodium takes place. The first signs of spore differentiation are found in late aggregates, where prespore cells can be distinguished from the surrounding undifferentiated cells by the increased electron density of their cytoplasm. Vacuoles comparable with the prespore vacuole of D. discoideum appear in both cell types; they fuse with the plasma membrane during sporulation of electron-dense cells and are lysed in electron-light cells, which eventually form the stalk. In contrast with D. discoideum no spatial separation between prespore and prestalk cells is found until very late in fruiting body development. 相似文献
8.
A mathematical continuum model of the slime mold Dictyostelium discoideum in the morphogenetic development stage is presented, which represents the amoebae as a fluid with surface tension, acted upon by a body force due to the presence of a chemical attractant. Assuming that the amoebae at a given time are in quasi-equilibrium, and that the shape of the organism is prescribed, it is possible to calculate from the model plausible concentration and source-sink distributions that are consistent with the given shape. Typical observed shapes are accounted for by the presence of very large concentrations and concentration gradients of the chemical attractant at the top of the structure. 相似文献
9.
Extracts of cohesive cells of four species of cellular slime mold, D. mucoroides, D. purpureum, D. rosarium and P. violaceum agglutinate erythrocytes in a manner that is similar to that previously observed with extracts of D. discoideum and P. pallidum. We determined inhibitory activity of a series of sugars on the agglutination activity of each of these extracts, using both semiquantitative and quantitative agglutination assays. The inhibitory potency of this series of sugars was distinct for each extract, although only slight differences were found between several species, especially D. discoideum and P. violaceum. A possible role of these agglutinins in species-specific cell cohesion is considered. 相似文献
10.
The intercellular water present in pellets of centrifuged cell suspensions of the slime mold Dictyostelium discoideum was measured at four stages of differentiation. 相似文献
11.
Plants and fungi possess an outwardly directed plasma membrane proton pump that may regulate intracellular pH. We provide the first demonstration that amoebae of the slime mould Dictyostelium discoideum also possess a similar proton pump. It can be assayed either as an ATPase activity in highly purified plasma membranes or as a proton pump, after solubilization and reconstruction into liposomes. The pump is inhibited by vanadate, diethylstilbestrol (DES) and miconazole but not by azide or ouabain. The proton pump described here may represent the target for the action of DES and miconazole, both of which have previously been shown to induce stalk cell formation during the in vitro development of Dictyostelium. 相似文献
12.
Complex I, a key component of the mitochondrial electron transport system, is thought to have evolved from at least two separate enzyme systems prior to the evolution of mitochondria from a bacterial endosymbiont, but the genes for one of the enzyme systems are thought to have subsequently been transferred to the nuclear DNA. We demonstrated that the cellular slime mold Dictyostelium discoideum retains the ancestral characteristic of having mitochondria encoding at least one gene (80-kDa subunit) that is nuclear encoded in other eukaryotes. This is consistent with the cellular slime molds of the family Dictyosteliaceae having diverged from other eukaryotes at an early stage prior to the loss of the mitochondrial gene in the lineage giving rise to plants and animals. The D. discoideum mitochondrially encoded 80-kDa subunit of complex I exhibits a twofold-higher mutation rate compared with the homologous chromosomal gene in other eukaryotes, making it the most divergent eukaryotic form of this protein.Correspondence to: K.L. Williams 相似文献
13.
Background
The life-cycle of cellular slime molds comprises chronobiologically regulated processes. During the growth phase, the amoeboid cells proliferate at a definite rate. Upon starvation, they synthesize cAMP as both first and second messengers in signalling pathways and form aggregates, migrating slugs, and fruiting bodies, consisting of spores and stalk cells, within 24 h. In Dictyostelium discoideum, because most growth-specific events cease during development, proliferative and heterochronic mutations are not considered to be interrelated and no genetic factor governing the entire life-cycle duration has ever been identified.Methodology/Principal Findings
Using yeast 2-hybrid library screening, we isolated a Dictyostelium discoideum RabGAP, Dd Rbg-3, as a candidate molecule by which the Dictyostelium Gα2 subunit directs its effects. Rab GTPase-activating protein, RabGAP, acts as a negative regulator of Rab small GTPases, which orchestrate the intracellular membrane trafficking involved in cell proliferation. Deletion mutants of Dd rbg-3 exhibited an increased growth rate and a shortened developmental period, while an overexpression mutant demonstrated the opposite effects. We also show that Dd Rbg-3 interacts with 2 Gα subunits in an activity-dependent manner in vitro. Furthermore, both human and Caenorhabditis elegans rbg-3 homologs complemented the Dd rbg-3–deletion phenotype in D. discoideum, indicating that similar pathways may be generally conserved in multicellular organisms.Conclusions/Significance
Our findings suggest that Dd Rbg-3 acts as a key element regulating the duration of D. discoideum life-span potentially via trimeric G-protein cascades. 相似文献14.
In migrating pseudoplasmodia of the cellular slime mold Dictyostelium discoideum, cells in approximately the anterior quarter of the structure give rise to stalk cells, while the remainder produce spore cells. Certain biochemical and structural components have been found to differ between cells occupying these two positions, indicating that some differentiation has already occurred by this stage. To evaluate the degree of this differentiation we have compared the proteins being synthesized in different regions of the pseudoplasmodia. Migrating pseudoplasmodia were labeled with [35S]methionine and cut into five segments, and the labeled proteins were resolved by two-dimensional gel electrophoresis and visualized by autofluorography. Of 500 polypeptides detected, 57 showed regional variations in labeling. Nearly all of these differences in labeling occurred between the anterior fifth and posterior four-fifths of the pseudoplasmodia, indicating that by this stage a marked degree of differentiation has occurred between the two cell types. 相似文献
15.
Serological investigations of cell adhesion in the slime molds,Dictyostelium discoideum,Dictyostelium purpureum,and Polysphondylium violaceum
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GREGG JH 《The Journal of general physiology》1956,39(5):813-820
1. Antibodies to slime molds were produced by injecting D. discoideum and D. purpureum amebas from 48 hour cultures into rabbits. 2. Anti-D. discoideum and anti-D. purpureum sera caused agglutination of homologous amebas from 24 to 26 hour cultures, agglutination of certain heterologous amebas from 30 to 36 hour cultures, and agglutination of all heterologous amebas from 43 to 48 hour cultures. 3. The data show that new surface antigens are formed in cultures after 26 hours and it is suggested that the new antigens are concerned with cell adhesion. 4. The probable role of surface antigens in the interaction of cells of different species of slime molds was discussed. 相似文献
16.
The cell cycle and its relationship to aggregation in the cellular slime mold, Dictyostelium discoideum 总被引:1,自引:0,他引:1
Vegetative amoebae of the cellular slime mold Dictyostelium discoideum were synchronized by the use of a temperature-sensitive mutant. The synchronized population was then used to analyze the cell cycle in Dictyostelium discoideum. This in turn enabled us to study the relationship between specific stages of the cell cycle and the initiation of aggregation. It was shown that all cells are at the same position (midway in G2) at the time of aggregation. Synchronous cells starved at all points in the cell cycle, however, took the same length of time to aggregate. This suggests that the limiting step in the aggregation process is starvation, which is independent of the position of the cells in the cell cycle. 相似文献
17.
Escherichia coli and the cellular slime mold Dictyostelium discoideum form stable viscous symbiotic colonies in the laboratory. To examine changes in E. coli gene expression during establishment of this symbiotic relationship, cells of symbiotic co-cultures and monocultures at various time points were subjected to microarrays analysis. Genes changed significantly over time compared to the initial gene expression level were determined as characteristics of GO function categories. The categories that appeared significantly at the same sampling time points between the two cultures were also identified. Up-regulation of genes from several GO categories associated with polysaccharide synthesis, cell wall degradation, and iron acquisition as well as down-regulation of genes from GO categories associated with biosynthesis through starvation response were observed in co-cultures, indicating exchange of molecules between the two organisms. Up-regulation of genes from several GO categories associated with anaerobic respiration and flagella biosynthesis were also observed, indicating that the environment inside symbiotic colonies was similar to that in developed biofilms. Up-regulation of genes associated with energy-generating systems indicated that E. coli prolonged survival within the symbiotic colony. Thus, E. coli showed not only molecule exchange but also altered expression of various genes in symbiosis with D. discoideum. 相似文献
18.
The slime mold Dictyostelium discoideum is one of the model systems of biological pattern formation. One of the most successful answers to the challenge of establishing a spiral wave pattern in a colony of homogeneously distributed D. discoideum cells has been the suggestion of a developmental path the cells follow (Lauzeral and coworkers). This is a well-defined change in properties each cell undergoes on a longer time scale than the typical dynamics of the cell. Here we show that this concept leads to an inhomogeneous and systematic spatial distribution of spiral waves, which can be predicted from the distribution of cells on the developmental path. We propose specific experiments for checking whether such systematics are also found in data and thus, indirectly, provide evidence of a developmental path. 相似文献
19.
Inhibition by Actinomycin D of Uridine Diphosphoglucose Synthetase Activity During Differentiation of Dictyostelium discoideum 总被引:1,自引:0,他引:1
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Actinomycin D inhibits the increase in specific activity of uridine diphosphoglucose synthetase during differentiation of the cellular slime mold, Dictyostelium discoideum. The degree to which this inhibition occurs is strictly correlated with, and may result from, the general inhibition of morphogenesis by actinomycin D. 相似文献
20.
Edmund V. Crean Edward F. Rossomando 《Biochimica et Biophysica Acta (BBA)/General Subjects》1977,498(2):439-441
A membrane fraction isolated from the cellular slime mold Dictyostelium discoideum was incubated with GDP-[14C]mannose and found to catalyze the incorporation of [14C]mannose into an endogenous acceptor to yield a product with the chemical and chromatographic properties of a polyprenol phosphate sugar derivative. These result suggest that D. discoideum can synthesize a mannosyl phosphoryl polyprenol. 相似文献