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1.
17α-ethinylestradiol cometabolism by bacteria degrading estrone, 17β-estradiol and estriol 总被引:2,自引:0,他引:2
Pauwels B Wille K Noppe H De Brabander H Van de Wiele T Verstraete W Boon N 《Biodegradation》2008,19(5):683-693
17alpha-ethinylestradiol (EE2), the active compound of the contraceptive pill, is a recalcitrant estrogen, which is encountered at ng/l levels in wastewater treatment plant (WWTP) effluents and rivers and can cause feminization of aquatic organisms. The aim of this study was to isolate micro-organisms that could remove such low EE2 concentrations. In this study, six bacterial strains were isolated from compost that cometabolize EE2 when metabolizing estrone (E1), 17beta-estradiol (E2) and estriol (E3). The strains belong to the alpha, beta and gamma-Proteobacteria. All six strains metabolize E2 over E1, at mug/l to ng/l concentrations. In 4 days, initial concentrations of 0.5 mug E2/l and 0.6 mug EE2/l were degraded to 1.8 +/- 0.4 ng E2/l and 85 +/- 16 ng EE2/l, respectively. No other metabolites besides E1, E2, E3 or EE2 were detected, suggesting that total degradation and cleavage of the aromatic ring occurred. This is the first study describing that bacteria able to metabolize E2, can subsequently cometabolize EE2 at low mug/l levels. 相似文献
2.
Estrone (E1), 17β-estradiol (E2) and 17α-ethynylestradiol (EE2) discharged from sewage treatment plants (STPs) into surface
waters, are seen as a threat effecting aquatic life by its estrogenic character. Therefore, much research is conducted on
the fate and removal of these compounds. Since these compounds are present in influents and effluents in the ng/l range, methods
for detection deserve special attention. Most important processes that play a role in the removal of estrogens are: adsorption,
aerobic degradation, anaerobic degradation, anoxic biodegradation and photolytic degradation. Halflifes tend to vary and are
remarkably shorter when low initial concentrations are applied. In general anaerobic conditions result in longer halflifes
then aerobic conditions. EE2 shows far most persistence of the compounds, thereby also the estrogenic effect in vitro is about 2–3-fold higher compared to E2. The three compounds show a higher affinity to sorb to sludge compared to other tested
adsorption materials like sediment. Aerobic degradation is far the most efficient in removing these compounds, but adsorption
seems to play a significant role in retaining the estrogens inside full-scale STPs. Removal rates in full scale plants depend
on the HRT, SRT and loading rates, but lack of information on the exact dependency so far prevents an optimal design able
to fully eliminate estrogens from wastewater. 相似文献
3.
Silvana Casati 《Steroids》2009,74(2):250-130
Boldenone is an androgenic anabolic steroid intensively used for growth promoting purposes in animals destined for meat production and as a performance enhancer in athletics. Therefore its use is officially banned either in animals intended for consumption or in humans. Because most anabolic steroids are completely metabolized and usually no parent steroid is excreted, metabolite identification is crucial to detect the illegal use of anabolic steroids either in humans or in livestock. 17α- and 17β-boldenone 17-glucuronides were synthesized, purified and characterized in order to provide suitable standards for the identification and quantification of these metabolites. 相似文献
4.
Th17 cells, not IL-17+ γδ T cells, drive arthritic bone destruction in mice and humans 总被引:1,自引:0,他引:1
Pöllinger B Junt T Metzler B Walker UA Tyndall A Allard C Bay S Keller R Raulf F Di Padova F O'Reilly T Horwood NJ Patel DD Littlewood-Evans A 《Journal of immunology (Baltimore, Md. : 1950)》2011,186(4):2602-2612
The mechanism whereby IL-17 drives rheumatoid arthritis remains incompletely understood. We demonstrate that anti-IL-17 therapy in collagen-induced arthritis ameliorates bone damage by reducing the number of osteoclasts in joints. We found equal numbers of CD4(+) Th17 and IL-17 producing γδ T cells in the joints of arthritic mice, and in vitro, both populations similarly induced osteoclastogenesis. However, individual depletion and adoptive transfer studies revealed that in vivo, Th17 cells dominated with regard to bone destruction. Unlike γδ T cells, Th17 cells were found in apposition to tartrate-resistant acid phosphatase positive osteoclasts in subchondral areas of inflamed joints, a pattern reproduced in patient biopsies. This localization was caused by Ag-specific retention, because OVA-primed Th17 cells showed a γδ T cell-like diffuse distribution. Because IL-23, as produced by osteoclasts, enhanced T cell-mediated osteoclastogenesis, we propose that Ag-specific juxtaposition is key to foster the molecular cross talk of Th17 cells and osteoclasts, thus driving arthritic bone destruction. 相似文献
5.
Petra Persson Sigurdur Hilmir Johannsson Yasuaki Takagi Björn Thrandur Björnsson 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1997,167(7):468-473
The effects of estradiol-17β (E2) on bone resorption and formation as well as its effects on scale resorption were investigated in rainbow trout in order
to elucidate the role of the hormone in calcium mobilization from calcified tissues, and to clarify the importance of scale
and bone as calcium reserves during sexual maturation. Furthermore, the effects of nutritional status on calcified tissues
and E2-induced calcium mobilization were studied. In fed as well as fasted rainbow trout, E2 treatment increased scale osteoclastic activity measured as tartrate-resistant acid phosphatase activity, and reduced scale
calcium content, suggesting that E2 increases scale resorption in both the fed and fasted fish. Using histomorphometry, E2 treatment was found to decrease pharyngeal bone resorption in fed, but not in fasted rainbow trout. The E2 effect on rainbow trout bone is consistent with its physiological role in mammals and birds where E2 has been reported to decrease bone resorption. It appears therefore that rainbow trout protect their skeleton and instead
use scales as a source of calcium during E2-induced calcium mobilization. The formation of pharyngeal bone was decreased by fasting, and the importance of the nutritional
status for the activity of the bone cells in rainbow trout is therefore emphasized.
Accepted: 8 May 1997 相似文献
6.
《Bioscience, biotechnology, and biochemistry》2013,77(3):411-414
Structure 1 is proposed for the Inagami-Tamura endogenous digitalis-like factor (EDLF), and (14β,17α)-14-hydroxy- and (14β, 17α)-2,14-dihydroxyestradiols (2 and 3) were synthesized as models for studies on 1. The latter compound was remarkably potent in inducing a contractile response in isolated rat aorta and guinea pig left atrium. 相似文献
7.
The present study evaluated protein oxidation, alteration in hydroxysteroid dehydrogenases (3β- and 17β HSD) in testes and serum hormonal profiles of dietary zinc deficient Wistar rats. Pre-pubertal rats were divided into three groups: zinc control (ZC), pairfed (PF), and zinc deficient (ZD) and fed 100 ppm (ZC and PF groups) and 1.0 ppm (ZD group) zinc diet for 2- and 4-weeks. The testes from zinc deficient groups exhibited significant increase in total protein (2 weeks) and protein carbonyl (2- and 4-weeks) concentration as well as 3β- and 17β-hydroxysteroid dehydrogenase activities (4 weeks), whereas a significant decrease was recorded in total protein (testes 4 weeks; serum 2- and 4-weeks), total zinc (testes and serum 2- and 4-weeks), 3β- and 17β-hydroxysteroid dehydrogenase activities (testes 2 weeks), and serum hormonal profiles (FSH and testosterone 2- and 4-weeks). However, LH was below the detectable limits. These results reflect that zinc deficiency during pre-pubertal period affected total protein and zinc status, elevates protein oxidation, and causes dysregulation of the hydroxysteroid dehydrogenases. Low level of zinc attenuated the gonadal physiology which indicates that the metabolic regulation of testes is mediated by combined effects of a specific response (caused by decreased zinc concentration) and a nonspecific response (inhibition of gonadotrophin secretion). All these contribute to testicular dysfunction. 相似文献
8.
Announcement
Capture, mark and re-capture workshop Hannover 16–17 March 2006 相似文献9.
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Theonellamide A, a bicyclic peptide isolated from a Theonella sponge, was fixed on hydrazide-containing gel beads and screened for its binding proteins from rabbit liver tissues. Analysis
by sodium dodecyl sulfate–polyacrylamide gel electrophoresis revealed that two major proteins of 80 kDa and 55 kDa interacted
with theonellamide A. The interaction between theonellamide A and two proteins was confirmed by competition experiments in
which these two proteins failed to bind to theonellamide A–conjugated gel beads in the presence of theonellamide A or F. Amino-terminal
amino acid sequence analysis of peptide fragments derived from the binding proteins by lysylendopeptidase digestion demonstrated
that the 80-kDa and 55-kDa proteins were 17β-hydroxysteroid dehydrogenase IV and glutamate dehydrogenase, respectively. In
an in vitro assay system, amination of α-ketoglutarate by glutamate dehydrogenase was activated with theonellamide F, although
this effect was weaker than that with adenosine diphosphate, a well-known activator.
Received October 15, 1999; accepted January 4, 2000. 相似文献
12.
Douglas C. McFarland Jane E. Pesall Cynthia S. Coy Sandra G. Velleman 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2013,164(4):565-571
The hypothesis of this study was that 17β-estradiol (estradiol) stimulates turkey skeletal muscle growth by influencing myogenic satellite cell proliferation, differentiation, and the gene expression of selected proteins important in regulating growth and development. Increasing levels of estradiol were administered in basal medium containing additional nutrients. Female-derived pectoralis major (PM) satellite cell proliferation was stimulated by estradiol at a level of 10? 9 M following 4 days of treatment. Male PM and biceps femoris (BF) satellite cell proliferation was increased at 10? 12 M estradiol. Turkey embryonic myoblast proliferation, however, decreased with 10? 9 M and 10? 5 M estradiol following 3 days under these conditions. Estradiol had no effect on the differentiation of any of the 4 groups of cells. Likewise, glypican-1 expression was unaffected by estradiol treatment. MyoD expression decreased in male PM but not BF cells. MyoD expression in female PM cells and embryonic myoblasts were also unaffected by estradiol administration. Estradiol decreased myogenin expression in male satellite cells, but had no effect on female cells. There was a slight decrease in myogenin expression in embryonic myoblasts. The results demonstrate a direct effect of estradiol on avian satellite cell proliferation independent of glypican-1, and decreased expression of MyoD and myogenin in some myogenic cells, coinciding with increased cellular proliferation. 相似文献
13.
Mahmoud Basant Abdel-Moneim Adel Negeem Zinab Nabil Ahmed 《Molecular biology reports》2022,49(5):3803-3809
Molecular Biology Reports - Diabetic nephropathy (DN) is among the main complications of diabetes mellitus and has been a major factor of renal failure. This study was designed to address the... 相似文献
14.
《Animal reproduction science》1987,14(3):205-218
Concentrations of luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin (PRL) were measured in jugular blood and those of oestradiol-17β (E217β) in utero-ovarian blood. Samples were taken from five intact gilts every 15 min for 108 h starting between day 15 and day 18 of the oestrous cycle. In the late luteal/early follicular phase, high pulsatile LH secretion, close to one pulse per hour, was observed. This could be the stimulus necessary for the final maturation of the ovarian follicles.Thereafter, frequency and amplitude of pulses, and the baseline value, decreased and were low at least between 36 and 12 h before the preovulatory LH surge. PRL and FSH concentrations also declined. This was probably due to the increase of oestrogen secretion. As E217β concentrations were still high, the surge of LH which was accompanied by increase in FSH and PRL, occurred for approximately 13 to 20 h. While LH and PRL mean levels decreased, FSH concentrations continued to increase. Peaks of PRL were observed during the late luteal/early follicular phase and during the LH discharge. During the period of estrus, each exposure to the boar was immediately followed by one of these peaks, which could play a role in the sexual behavior of the gilt. 相似文献
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16.
Thermal and hydrothermal biochars were characterized, and adsorption of bisphenol A (BPA), 17α-ethinyl estradiol (EE2) and phenanthrene (Phen) was determined to investigate the sorption characteristic difference between the two types of biochars. Thermal biochars were composed mostly of aromatic moieties, with low H/C and O/C ratios as compared to hydrothermal ones having diverse functional groups. Single-point organic carbon-normalized distribution coefficients (logKOC) of EE2 and BPA of hydrothermal biochars were higher than thermal biochars, while Phen logKOC values were comparable among them. X-ray diffraction and solid state nuclear magnetic resonance results suggested that hydrothermal biochars consisted of more amorphous aliphatic-C, possibly being responsible for their high sorption capacity of Phen. This study demonstrated that hydrothermal biochars could adsorb a wider spectrum of both polar and nonpolar organic contaminants than thermally produced biochars, suggesting that hydrothermal biochar derived from poultry and animal waste is a potential sorbent for agricultural and environmental applications. 相似文献
17.
Xiaojing Xu Shaoyong Su Vernon A. Barnes Carmen De Miguel Jennifer Pollock Dennis Ownby Huidong Shi Haidong Zhu Harold Snieder Xiaoling Wang 《Epigenetics》2013,8(5):522-523
Keystone Symposia kicked off the start of 2012 with two joint meetings on Epigenomics and Chromatin Dynamics and a star-studded list of speakers. Held in Keystone, CO, January 17–22, and organized by Steven Jacobsen and Steven Henikoff and by Bradley Cairns and Geneviève Almouzni, respectively, there was plenty happening in these sessions that it did not seem to matter that the ski-slope conditions were not ideal. 相似文献
18.
Marchais-Oberwinkler S Henn C Möller G Klein T Negri M Oster A Spadaro A Werth R Wetzel M Xu K Frotscher M Hartmann RW Adamski J 《The Journal of steroid biochemistry and molecular biology》2011,125(1-2):66-82
17β-Hydroxysteroid dehydrogenases (17β-HSDs) are oxidoreductases, which play a key role in estrogen and androgen steroid metabolism by catalyzing final steps of the steroid biosynthesis. Up to now, 14 different subtypes have been identified in mammals, which catalyze NAD(P)H or NAD(P)(+) dependent reductions/oxidations at the 17-position of the steroid. Depending on their reductive or oxidative activities, they modulate the intracellular concentration of inactive and active steroids. As the genomic mechanism of steroid action involves binding to a steroid nuclear receptor, 17β-HSDs act like pre-receptor molecular switches. 17β-HSDs are thus key enzymes implicated in the different functions of the reproductive tissues in both males and females. The crucial role of estrogens and androgens in the genesis and development of hormone dependent diseases is well recognized. Considering the pivotal role of 17β-HSDs in steroid hormone modulation and their substrate specificity, these proteins are promising therapeutic targets for diseases like breast cancer, endometriosis, osteoporosis, and prostate cancer. The selective inhibition of the concerned enzymes might provide an effective treatment and a good alternative to the existing endocrine therapies. Herein, we give an overview of functional and structural aspects for the different 17β-HSDs. We focus on steroidal and non-steroidal inhibitors recently published for each subtype and report on existing animal models for the different 17β-HSDs and the respective diseases. Article from the Special issue on Targeted Inhibitors. 相似文献
19.
Smirnov A. N. Pokrovskaya E. V. Shevchenko V. P. Nagaev I. Yu. Myasoedov N. F. Levina I. S. Kulikova L. E. Kamernitsky A. V. 《Russian Journal of Bioorganic Chemistry》2002,28(3):224-230
The binding of [3H]progesterone and [3H]16,17-cycloalkanoprogesterones to proteins from rat, rabbit, and human uteri and other organs was studied. We found that 16,17-cycloalkanoprogesterone derivatives display affinities for the uterine progesterone receptors comparable with that of the natural hormone and no substantial species differences in the affinity. Rabbit uterus was found to have no proteins distinct from the progesterone receptor that specifically bind [3H]16,17-cycloalkanoprogesterones. At the same time, in the human uterus, we found another protein that binds some of these progesterone derivatives; it turned out to be similar to the protein from rat uterus. A similar protein with the same selectivity and affinity for steroids was also found in rat and human kidneys. Blood serum, liver, lung, and a number of other tissues were found to contain a protein of the third type that binds the same 16,17-cycloalkanoprogesterones and exhibits submicromolar K
d values for these steroids and a very low affinity for progesterone. We speculated that the introduction of a bulky substituent adjacently to the 17-side chain of progesterone could result in a change in the general biodynamics of the derivative including its transport, uptake, and accumulation in tissues, which may determine the selectivity of its effect. 相似文献
20.
Samira Osati Hasrat Ali Fernanda Marques Michel Paquette Simon Beaudoin Brigitte Guerin Jeffrey V. Leyton Johan E. van Lier 《Bioorganic & medicinal chemistry letters》2017,27(3):443-446
In vivo imaging of estrogen receptor (ER) densities in human breast cancer is a potential tool to stage disease, guide treatment protocols and follow-up on treatment outcome. Both positron emission tomography (PET) and fluorescence imaging have received ample attention to detect ligand-ER interaction. In this study we prepared BODIPY-estradiol conjugates using 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) as fluorescent probe and estradiol derivatives as ligand and established their relative binding affinity (RBA) for the ERα. The synthesis of the conjugates involves attachment of a BODIPY moiety to the C17α-position of estradiol using Sonogashira or click reactions of iodo-BODIPY or aza-BODIPY with various 17α-ethynylestradiol (EE2) derivatives. The highest RBA for the ERα was observed with the EE2-BODIPY conjugate (7) featuring a linear eight carbon spacer chain. Cell uptake studies and in vivo imaging experiments in an ER-positive mouse tumor model are in progress. 相似文献